345 - Timstar
... THE POLYMERASE CHAIN REACTION (PCR) The PCR reaction is a DNA amplification technique that revolutionized almost all aspects of biological research. The procedure was invented by Dr. Kary Mullis while at Cetus Corporation in 1984. Dr. Mullis was awarded a Nobel Prize for his work in 1994. PCR amplif ...
... THE POLYMERASE CHAIN REACTION (PCR) The PCR reaction is a DNA amplification technique that revolutionized almost all aspects of biological research. The procedure was invented by Dr. Kary Mullis while at Cetus Corporation in 1984. Dr. Mullis was awarded a Nobel Prize for his work in 1994. PCR amplif ...
Transposable elements in Escherichia coli antimicrobial resistance
... Since they have the intrinsic ability to multiply during the transposition process, they are automatically amplified, with a large copy number, restricted only by the carrying capacity of their environment (i.e. the genome) [1]. Transposons often persist in genomes over millions of years. This requi ...
... Since they have the intrinsic ability to multiply during the transposition process, they are automatically amplified, with a large copy number, restricted only by the carrying capacity of their environment (i.e. the genome) [1]. Transposons often persist in genomes over millions of years. This requi ...
A novel arginine substitution mutation in 1A domain and a novel 27
... black, mutant sequences in red. Regions of duplication are in capital letters. Insertions of duplications into mutant sequences are boxed. (F) Amino acid sequences. Duplication of sequence first seen at codon position 400. ...
... black, mutant sequences in red. Regions of duplication are in capital letters. Insertions of duplications into mutant sequences are boxed. (F) Amino acid sequences. Duplication of sequence first seen at codon position 400. ...
JunctionSeq Package User Manual
... Under the default parameterization, JunctionSeq also builds upon and expands the basic design put forth by DEXSeq, providing (among other things) the ability to test for both differential exon usage and differential splice junction usage. These two types of analyses are complementary: Exons represen ...
... Under the default parameterization, JunctionSeq also builds upon and expands the basic design put forth by DEXSeq, providing (among other things) the ability to test for both differential exon usage and differential splice junction usage. These two types of analyses are complementary: Exons represen ...
Mutualism and asexual reproduction influence recognition genes in a fungal... M.A. van der Nest1, E.T. Steenkamp2, P.M.... Wingfield1 and B. Slippers1*
... costly than asexual reproduction (e.g., Barton and Charlesworth 1998; Otto 2003). This costly reproductive strategy could be maintained because recombination acts to provide advantageous genotypes necessary for adaptation to changing environments or because recombination acts to eliminate deleteriou ...
... costly than asexual reproduction (e.g., Barton and Charlesworth 1998; Otto 2003). This costly reproductive strategy could be maintained because recombination acts to provide advantageous genotypes necessary for adaptation to changing environments or because recombination acts to eliminate deleteriou ...
Identifying Common Genetic Variants by High
... detected variants (7, 8, 11, 13 ). Indeed, heteroduplex scanning methods as a rule detect sequence variants but do not identify or genotype those variants. Common sequence variants that do not cause disease occur at a frequency much greater than that of disease-causing variants. These common variant ...
... detected variants (7, 8, 11, 13 ). Indeed, heteroduplex scanning methods as a rule detect sequence variants but do not identify or genotype those variants. Common sequence variants that do not cause disease occur at a frequency much greater than that of disease-causing variants. These common variant ...
The first page should show the paper title, names and addresses of
... chromosomal rearrangements that might have occurred during karyotype evolution in Galliformes could be resolved using the FISH on lampbrush chromosomes as an investigative procedure? On the other hand, why should we still use time-consuming methods like comparative FISH mapping on LBCs when next-gen ...
... chromosomal rearrangements that might have occurred during karyotype evolution in Galliformes could be resolved using the FISH on lampbrush chromosomes as an investigative procedure? On the other hand, why should we still use time-consuming methods like comparative FISH mapping on LBCs when next-gen ...
Prediction and investigation of novel proteins in DNA double
... broken ends to close proximity and Dnl4/Lif1/Nej1 ligates the DNA ends. Protein-protein interaction (PPI) has been utilized in functional genomics studies to identify novel proteins involved in different pathways based on their PPI profiles. In this study we aim to screen for novel proteins involved ...
... broken ends to close proximity and Dnl4/Lif1/Nej1 ligates the DNA ends. Protein-protein interaction (PPI) has been utilized in functional genomics studies to identify novel proteins involved in different pathways based on their PPI profiles. In this study we aim to screen for novel proteins involved ...
A mixed group ll/group III twintron in the Euglena
... gracilis chloroplast ribosomal protein S3 gene (rps3) was examined by cDNA cloning and sequencing, and northern hybridization. Based on the characterization of a partially spliced pre-mRNA, the intron was characterized as a 'mixed' twintron, composed of a 311 nucleotide group II intron internal to a ...
... gracilis chloroplast ribosomal protein S3 gene (rps3) was examined by cDNA cloning and sequencing, and northern hybridization. Based on the characterization of a partially spliced pre-mRNA, the intron was characterized as a 'mixed' twintron, composed of a 311 nucleotide group II intron internal to a ...
Gene silencing in mammalian cells and the spread of DNA
... Aprt promoter and the methylation center is methylated on approximately 25% of alleles, and that this level persisted even when the cells were subcloned (Turker et al., 1989). These observations can best be explained by assuming that methylation for CpG sites on a given allele can be either gained o ...
... Aprt promoter and the methylation center is methylated on approximately 25% of alleles, and that this level persisted even when the cells were subcloned (Turker et al., 1989). These observations can best be explained by assuming that methylation for CpG sites on a given allele can be either gained o ...
Comparative Metagenomic Analysis Reveals Mechanisms for Stress
... from 45 ‘seed’ genes related to stress response obtained from the GeoChip database (Tu et al. 2014). Only genes related to stress response, including genes that belong to any one of five functional ‘stress’ categories: temperature, osmolarity, oxidative stress and oxygen limitation, nutrient limitat ...
... from 45 ‘seed’ genes related to stress response obtained from the GeoChip database (Tu et al. 2014). Only genes related to stress response, including genes that belong to any one of five functional ‘stress’ categories: temperature, osmolarity, oxidative stress and oxygen limitation, nutrient limitat ...
January 2007; Phenocore Findings; Vol 1, issue 1
... normal mouse phenotypes, and to new and established phenotyping capabilities in multiple disciplines. Phenotyping expertise and resources at JHMI will be emphasized. Lecture topics will include but are not limited to development of genetically engineered mice (and other animals), traditional and nov ...
... normal mouse phenotypes, and to new and established phenotyping capabilities in multiple disciplines. Phenotyping expertise and resources at JHMI will be emphasized. Lecture topics will include but are not limited to development of genetically engineered mice (and other animals), traditional and nov ...
Genome-wide analysis by SNP Array
... from one parent’s chromosome with silencing of the other parent’s chromosome. In the case of PWS, the paternal region is active, so that deletion or failure of inheritance of the paternal region causes the syndrome. PWS is characterised by mental retardation, hypogonadism, hypotonia, obesity, charac ...
... from one parent’s chromosome with silencing of the other parent’s chromosome. In the case of PWS, the paternal region is active, so that deletion or failure of inheritance of the paternal region causes the syndrome. PWS is characterised by mental retardation, hypogonadism, hypotonia, obesity, charac ...
Supplemental Material
... based on the sequence of the scaffolds and in situ hybridizations were performed. A unique approach was adopted for the Hawaiian species D. grimshawi. In this case, there were several prior localizations of individual genes, but these were done on Hawaiian species other than D. grimshawi (DAVIS et a ...
... based on the sequence of the scaffolds and in situ hybridizations were performed. A unique approach was adopted for the Hawaiian species D. grimshawi. In this case, there were several prior localizations of individual genes, but these were done on Hawaiian species other than D. grimshawi (DAVIS et a ...
Marine integrons containing novel integrase genes
... In order to understand the structure and biological significance of integrons and associated gene cassettes in marine polluted sediments, metagenomic DNAs were extracted from sites at Suez and Tokyo Bays. PCR amplicons containing new integrase genes, intI, linked with novel gene cassettes, were reco ...
... In order to understand the structure and biological significance of integrons and associated gene cassettes in marine polluted sediments, metagenomic DNAs were extracted from sites at Suez and Tokyo Bays. PCR amplicons containing new integrase genes, intI, linked with novel gene cassettes, were reco ...
Nixon Evidence
... encoding fermentation enzymes and related electron transport peptides (e.g., ferredoxins) in giardia organisms and amebae are hypothesized to be derived from either an ancient anaerobic eukaryote (amitochondriate fossil hypothesis), a mitochondrial endosymbiont (hydrogen hypothesis), or anaerobic ba ...
... encoding fermentation enzymes and related electron transport peptides (e.g., ferredoxins) in giardia organisms and amebae are hypothesized to be derived from either an ancient anaerobic eukaryote (amitochondriate fossil hypothesis), a mitochondrial endosymbiont (hydrogen hypothesis), or anaerobic ba ...
ADP Ribosylation Factor is an Essential Protein in Saccharomyces cerevisiae and is Encoded by Two Genes.
... certain restriction sites within the coding sequences are conserved, possibly explaining the failure to identify ARF2 in the experiments in which ARFI was originally cloned (39). The predicted ARF2 protein is identical in length to the ARF1 protein and 96% identical in protein sequence, differing at ...
... certain restriction sites within the coding sequences are conserved, possibly explaining the failure to identify ARF2 in the experiments in which ARFI was originally cloned (39). The predicted ARF2 protein is identical in length to the ARF1 protein and 96% identical in protein sequence, differing at ...
Physical map of the aromatic amine and m-toluate
... present in Pseudomonasputida UCC22, a derivativeof P.putida mt-2. The plasmid is 79 1kbp in size and can be divided into a restriction-site-deficient region of 51 f 1 kbp and a restriction-site-profuse region of 28 kbp which begins and ends with directly repeating sequences of at least 2 kbp in leng ...
... present in Pseudomonasputida UCC22, a derivativeof P.putida mt-2. The plasmid is 79 1kbp in size and can be divided into a restriction-site-deficient region of 51 f 1 kbp and a restriction-site-profuse region of 28 kbp which begins and ends with directly repeating sequences of at least 2 kbp in leng ...
Molecular cloning and biochemical characterization of a
... digested with EcoRI. After EcoRI digestion, high molecular weight DNA was fractionated on a sucrose gradient and used to prepare a genomic library in lambda EMBL 4 (Stratagene) according to the manufacturer's instructions. More than 2 105 phages were transferred to Hybond N nylon membrane (Amersha ...
... digested with EcoRI. After EcoRI digestion, high molecular weight DNA was fractionated on a sucrose gradient and used to prepare a genomic library in lambda EMBL 4 (Stratagene) according to the manufacturer's instructions. More than 2 105 phages were transferred to Hybond N nylon membrane (Amersha ...
induction of instability at selected loci in maize
... "INDUCTION OF INSTABILITY AT SELECTED LOCI IN MAIZE" Barbara McClintock ...
... "INDUCTION OF INSTABILITY AT SELECTED LOCI IN MAIZE" Barbara McClintock ...
GENOTYPE-PHENOTYPE CORRELATION USING
... Biological science has undergone a revolution in the past few decades. The successes of molecular and structural biology, biochemistry, and genetics have yielded large amounts of data that are increasingly quantitative in nature. This quantitative analysis of this data has attracted the use of techn ...
... Biological science has undergone a revolution in the past few decades. The successes of molecular and structural biology, biochemistry, and genetics have yielded large amounts of data that are increasingly quantitative in nature. This quantitative analysis of this data has attracted the use of techn ...
Gene conversion rapidly generates major histocompatibility complex
... code for cell surface glycoproteins that present pathogenic peptides to cells of the immune system, and so play a direct role in pathogen defence (Klein 1986). These genes display extraordinary levels of polymorphism, often with hundreds of alleles at individual loci (Garrigan & Hedrick 2003). Patte ...
... code for cell surface glycoproteins that present pathogenic peptides to cells of the immune system, and so play a direct role in pathogen defence (Klein 1986). These genes display extraordinary levels of polymorphism, often with hundreds of alleles at individual loci (Garrigan & Hedrick 2003). Patte ...
Transgenic approaches for optogenetics Tim Murphy UBC.
... sequences which flank an 8bp spacer region. The products of Cre-mediated recombination at loxP sites are dependent upon the location and relative orientation of the loxP sites. Two separate DNA species both containing loxP sites can undergo fusion as the result of Cre mediated recombination. DNA seq ...
... sequences which flank an 8bp spacer region. The products of Cre-mediated recombination at loxP sites are dependent upon the location and relative orientation of the loxP sites. Two separate DNA species both containing loxP sites can undergo fusion as the result of Cre mediated recombination. DNA seq ...
Standards and guidelines for the interpretation of sequence
... These Standards and Guidelines should not be considered inclusive of all proper procedures and tests or exclusive of other procedures and tests that are reasonably directed to obtaining the same results. In determining the propriety of any specific procedure or test, the clinical laboratory genetici ...
... These Standards and Guidelines should not be considered inclusive of all proper procedures and tests or exclusive of other procedures and tests that are reasonably directed to obtaining the same results. In determining the propriety of any specific procedure or test, the clinical laboratory genetici ...
GROW`N`GLOW: THE ACE1 TWO-HYBRID
... MoBiTec's ACE1-based Grow'n'Glow yeast two-hybrid system is a version of a system originally developed by Fields and Song1. The yeast two-hybrid system has proven to be a powerful tool for identifying proteins from an expression library which can interact with one's protein of interest. The technolo ...
... MoBiTec's ACE1-based Grow'n'Glow yeast two-hybrid system is a version of a system originally developed by Fields and Song1. The yeast two-hybrid system has proven to be a powerful tool for identifying proteins from an expression library which can interact with one's protein of interest. The technolo ...