Conservation, relocation and duplication in genome evolution
... genes from the X chromosome to the autosomes is expected only for dominant gain-of-function mutations [38], and it is difficult to argue that alleles favoring males are dominant in worms and flies but recessive in mammals. Alternative explanations on the basis of dosage compensation [41] and X-inact ...
... genes from the X chromosome to the autosomes is expected only for dominant gain-of-function mutations [38], and it is difficult to argue that alleles favoring males are dominant in worms and flies but recessive in mammals. Alternative explanations on the basis of dosage compensation [41] and X-inact ...
Slides
... to do. Science, 291(5507), 1186. – Technology of the time permitted 500 bp per day per person. – Move from radioactively labeled sequencing to fuorescent sequencing permitted complete automation up to 1 gigabyte per year. ...
... to do. Science, 291(5507), 1186. – Technology of the time permitted 500 bp per day per person. – Move from radioactively labeled sequencing to fuorescent sequencing permitted complete automation up to 1 gigabyte per year. ...
Normalization of gene expression measurements in tumor tissues
... (Ct) value. A measure of 10 ml of cDNA of each tissue sample (n ¼ 80) was added to 315 ml H2O and 325 ml Universal Master Mix (Applied Biosystems). This mixture was distributed over a row of 12 wells, with 50 ml mixture in each well. Consequently, 11 housekeeping genes and the APC sequence were ampl ...
... (Ct) value. A measure of 10 ml of cDNA of each tissue sample (n ¼ 80) was added to 315 ml H2O and 325 ml Universal Master Mix (Applied Biosystems). This mixture was distributed over a row of 12 wells, with 50 ml mixture in each well. Consequently, 11 housekeeping genes and the APC sequence were ampl ...
Slide 1
... sequence the fern genome of your 2. Could maintaining large amounts of choice. Which would you choose and why? physical genetic material be What methods would you use? disadvantageous for fern evolution? Could it be related to slow speciation 2. Why is the fate of most duplicate genes to rates, comp ...
... sequence the fern genome of your 2. Could maintaining large amounts of choice. Which would you choose and why? physical genetic material be What methods would you use? disadvantageous for fern evolution? Could it be related to slow speciation 2. Why is the fate of most duplicate genes to rates, comp ...
The new genes of rice: a closer look
... detail, with the help of experiments, to trap coding DNA and identify the non-coding DNA [21]. Second, in some cases, higher scores were given to rice gene candidates if the same prediction had already been made via a different method or by a different group. Such strategies could initiate a snowbal ...
... detail, with the help of experiments, to trap coding DNA and identify the non-coding DNA [21]. Second, in some cases, higher scores were given to rice gene candidates if the same prediction had already been made via a different method or by a different group. Such strategies could initiate a snowbal ...
Microarray experiment guidelines
... organism/cell/tissue – whether it be mutational studies (monitoring the effects of gene expression by knocking out/in a particular gene), conditional (monitoring the effects on gene expression when presenting the organism/cell to a particular environment/stress) and/or comparative (compare the prese ...
... organism/cell/tissue – whether it be mutational studies (monitoring the effects of gene expression by knocking out/in a particular gene), conditional (monitoring the effects on gene expression when presenting the organism/cell to a particular environment/stress) and/or comparative (compare the prese ...
Androgenic control of nucleic acid and protein synthesis in male
... to believe that the effects of androgens on protein synthesis in male accessory glands are in a n y large measure dictated by actions of these hormones on either the active transport of amino acids, or on the enzymatic synthesis of transfer RNAamino acids. Recent reviews by Riggs ('64) and Tomkins a ...
... to believe that the effects of androgens on protein synthesis in male accessory glands are in a n y large measure dictated by actions of these hormones on either the active transport of amino acids, or on the enzymatic synthesis of transfer RNAamino acids. Recent reviews by Riggs ('64) and Tomkins a ...
molecularevolution.rnaseq
... Goal: Identify regions enriched in the chromatin mark of interest Challenge: As we saw, Chromatin marks come in very different forms. ...
... Goal: Identify regions enriched in the chromatin mark of interest Challenge: As we saw, Chromatin marks come in very different forms. ...
HYBRID PETRI NET REPRESENTATION OF GENE
... equation models, including both mRNA and protein levels. They showed theoretically how to solve the problem of estimating the parameters on the models using linear algebra and Fourier transforms. Novak et al. 6 constructed a mathematical model of ssion yeast growth and division that encompasses al ...
... equation models, including both mRNA and protein levels. They showed theoretically how to solve the problem of estimating the parameters on the models using linear algebra and Fourier transforms. Novak et al. 6 constructed a mathematical model of ssion yeast growth and division that encompasses al ...
Lab 9: Regulation of lactose metabolism
... Lab 9: Regulation of lactose metabolism There are clearly different means and levels of regulating gene products, but the first comprehensive description of regulation of genes was that of the enzymes necessary for lactose metabolism in the bacterium, Escherichia coli. It has been known since the ea ...
... Lab 9: Regulation of lactose metabolism There are clearly different means and levels of regulating gene products, but the first comprehensive description of regulation of genes was that of the enzymes necessary for lactose metabolism in the bacterium, Escherichia coli. It has been known since the ea ...
Homeotic selector genes
... What is the mechanism that retains positional memory? – one good mechanism is positive feedback • once a gene product is expressed, it stimulates its own expression • many HOM-C genes have autoregulatory binding sites in their promoters – but positive feedback, in itself, is insufficient to maintain ...
... What is the mechanism that retains positional memory? – one good mechanism is positive feedback • once a gene product is expressed, it stimulates its own expression • many HOM-C genes have autoregulatory binding sites in their promoters – but positive feedback, in itself, is insufficient to maintain ...
Whole genome sequencing and assembly of an avian genome, the
... sequenced fragments and makes use of this information to place the different fragments with respect to each other. This method of genome assembly is known as ‘shotgun’ genome sequencing. In this project we tested the benefits of an alternative method for genome assembly called reduced representation ...
... sequenced fragments and makes use of this information to place the different fragments with respect to each other. This method of genome assembly is known as ‘shotgun’ genome sequencing. In this project we tested the benefits of an alternative method for genome assembly called reduced representation ...
Mixed questions
... mechanism is involved in the regulation of a biosynthetic pathway. 16. Explain the central dogma of molecular biology. 17. Gene expression can be regulated at several levels. Explain. 18. Gene expression can be regulated at the levels of transcription and translation. Discuss each with respect to en ...
... mechanism is involved in the regulation of a biosynthetic pathway. 16. Explain the central dogma of molecular biology. 17. Gene expression can be regulated at several levels. Explain. 18. Gene expression can be regulated at the levels of transcription and translation. Discuss each with respect to en ...
Developing a CRISPR/Cas9 System for Volvox Carteri
... targeted mutations. The Cas9/CRISPR system is simpler and more precise than previously developed genome editing systems. The high precision is due to the CRISPR associated (Cas) endonuclease’s ability to bind DNA via associated guide RNAs. Cas endonucleases can delete or add bases to the genome, whi ...
... targeted mutations. The Cas9/CRISPR system is simpler and more precise than previously developed genome editing systems. The high precision is due to the CRISPR associated (Cas) endonuclease’s ability to bind DNA via associated guide RNAs. Cas endonucleases can delete or add bases to the genome, whi ...
Bioinfo primer - part 6/6
... • DNA is a double-helix and can be separated by denaturing treatment into two strands. Each strand becomes ’sticky’ and attempts to renature with homologous single-strand sequences to form hybrids. • Single-strand DNA from all known genes of a given species can be attached to a matrix, then probed w ...
... • DNA is a double-helix and can be separated by denaturing treatment into two strands. Each strand becomes ’sticky’ and attempts to renature with homologous single-strand sequences to form hybrids. • Single-strand DNA from all known genes of a given species can be attached to a matrix, then probed w ...
Characterization of PIR1, a GATA family transcription factor involved
... the degradation of lignocellulose by this fungus (Kremer and Wood, 1992a,b; Backa et al., 1993; Wood, 1994; Henriksson et al., 1995; Tanaka et al., 1999). Fenton-based mechanisms have been suggested to be key in cellulose depolymerization by brown-rot fungi like Postia placenta (Baldrian and Valasko ...
... the degradation of lignocellulose by this fungus (Kremer and Wood, 1992a,b; Backa et al., 1993; Wood, 1994; Henriksson et al., 1995; Tanaka et al., 1999). Fenton-based mechanisms have been suggested to be key in cellulose depolymerization by brown-rot fungi like Postia placenta (Baldrian and Valasko ...
Why does the giraffe have such a long neck? Analysis zeroes in on
... Most of the genes Cavener and Agaba found help determine how the bodies of other animals form. This supports the idea that tweaking these genes could change an animal's physical qualities. For example, a small change in genes could make a donkey-like creature incredibly tall. The scientists found so ...
... Most of the genes Cavener and Agaba found help determine how the bodies of other animals form. This supports the idea that tweaking these genes could change an animal's physical qualities. For example, a small change in genes could make a donkey-like creature incredibly tall. The scientists found so ...
Transcription
... a general non-specific affinity for DNA, which is referred to as loose binding that is fairly stable. • The addition of s factor to the core enzyme markedly reduces the holoenzyme affinity for non-specific binding by 20 000-fold, and enhances the holoenzyme binding to correct promoter sites 100 time ...
... a general non-specific affinity for DNA, which is referred to as loose binding that is fairly stable. • The addition of s factor to the core enzyme markedly reduces the holoenzyme affinity for non-specific binding by 20 000-fold, and enhances the holoenzyme binding to correct promoter sites 100 time ...
A programme for the construction of a lambda phage
... necessary to activate transcription of most other phage genes. The cl gene codes for the lambda repressor, the regulatory protein that switches off transcription of prophage genes in the lysogenic state. The presence of the lambda repressor makes a A-lysogenic cell immune to superinfection by anothe ...
... necessary to activate transcription of most other phage genes. The cl gene codes for the lambda repressor, the regulatory protein that switches off transcription of prophage genes in the lysogenic state. The presence of the lambda repressor makes a A-lysogenic cell immune to superinfection by anothe ...
Biol115_2014_Lecture 10_Prokaryotic Gene Regulation
... Operons: The basic concept" • A cluster of functionally related genes can be under coordinated control by a single on-off “switch”" • An operon is the entire stretch of DNA that includes the operator, the promoter, and the genes that they control" • The regulatory “switch” is a segment of DNA ca ...
... Operons: The basic concept" • A cluster of functionally related genes can be under coordinated control by a single on-off “switch”" • An operon is the entire stretch of DNA that includes the operator, the promoter, and the genes that they control" • The regulatory “switch” is a segment of DNA ca ...
Role of microRNA in Skeleton Development
... the upregulation of osteogenic genes. In this context, it may be useful to look at the two roles played by the muscle-specific miR-133 in myocyte and osteoblast differentiation. In myocyte differentiation, miR-133 is up regulated downstream of the transcription factors myogenin, MyoD, SRF, and Mef2 ...
... the upregulation of osteogenic genes. In this context, it may be useful to look at the two roles played by the muscle-specific miR-133 in myocyte and osteoblast differentiation. In myocyte differentiation, miR-133 is up regulated downstream of the transcription factors myogenin, MyoD, SRF, and Mef2 ...
Nuclear Genes That Encode Mitochondrial Proteins
... sion of particular subgenomic DNA molecules to nearly undetectable levels during plant development. This process, first discovered in maize (Small et al., 1987), appears to be widespread in plants and may constitute a means of maintaining mitochondrial genetic variation in a silenced but retrievable ...
... sion of particular subgenomic DNA molecules to nearly undetectable levels during plant development. This process, first discovered in maize (Small et al., 1987), appears to be widespread in plants and may constitute a means of maintaining mitochondrial genetic variation in a silenced but retrievable ...
Analysis of GDSL lipase (GLIP) family genes in rice (Oryza sativa)
... Our analysis showed that 42 members were with high signal value (control group signal value > 100) for rice seedlings treated with different abiotic stresses (desiccation, salt and cold) as compared to mock-treated control (7-day-old seedlings) (Fig. 4), 8 were found to be up-regulated by more than ...
... Our analysis showed that 42 members were with high signal value (control group signal value > 100) for rice seedlings treated with different abiotic stresses (desiccation, salt and cold) as compared to mock-treated control (7-day-old seedlings) (Fig. 4), 8 were found to be up-regulated by more than ...