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Foundations of Biology - Geoscience Research Institute
Foundations of Biology - Geoscience Research Institute

... 2 LacY - Codes for a permease that lets lactose across the cell membrane 3 LacA - Transacetylase - An enzyme whose function in lactose metabolism is uncertain 4 Repressor - A protein that works with the control region to control expression of the operon ...
Chapter 16: Gene Regulation in Bacteria
Chapter 16: Gene Regulation in Bacteria

... worked out. Our initial understanding of gene regulation can be traced back to the 1950s and the creative minds of two French scientists: François Jacob and Jacques Monod. They were interested in the phenomenon of enzyme adaptation, which refers to the observation that a particular enzyme appears wi ...
video slide - Course
video slide - Course

... – Is an important factor in determining the protein synthesis in a cell – Is determined in part by sequences in the leader and trailer regions ...
Eukaryotic Genomes
Eukaryotic Genomes

... – Is an important factor in determining the protein synthesis in a cell – Is determined in part by sequences in the leader and trailer regions ...
Comparative genomics and the evolution of prokaryotes
Comparative genomics and the evolution of prokaryotes

... they already had a homolog present in the recipient genome. Another study confirmed the dominant role of HGT over duplication to the evolution of the E. coli metabolic network [31]. However, the relative role of HGT and duplication might vary significantly among species: recent studies of two large ...
here
here

... Prevent their associated proteins from being produced • histone acetylation • methylation • small RNA interference Sexual reproduction has been suggested to play a role in reducing the number of deleterious transposable elements ...
Expression analysis of a chicory fructan 1‐exohydrolase gene
Expression analysis of a chicory fructan 1‐exohydrolase gene

... were uprooted, defoliated, and stored for 3 weeks at 1 °C. While in storage, root samples were taken on 10 and 16 November. For all the other results, chicory was grown in a greenhouse and 3month-old plants used for all the experiments. To test the in¯uence of cold storage, 3-month-old roots were up ...
XistAR write up
XistAR write up

... and sequencing RNA/DNA of male rats, since no inactivation is required in male rats, Tsix is only expressed from that mutant allele (Figure 1b). However, in the female versions of these rats, there appears to be an antisense transcript present, which can only be transcribed from the WT inactivated X ...
Dosage compensation: do birds do it as well?
Dosage compensation: do birds do it as well?

... obviously contains many genes. For one of the nine genes analysed by quantitative RT–PCR, ScII, expression levels were consistently twice as high in males as in females [4]. This might suggest that some avian Z-linked genes escape dosage compensation in a similar manner to about 10% of X-linked huma ...
Down Syndrome: Antonarakis et al. (2004)
Down Syndrome: Antonarakis et al. (2004)

... species show amino-acid differences. Indels within coding regions represent one of the main mechanisms that lead to protein diversity. An interesting approach to improving the functional annotation of HSA21 involves studying transcriptional activity of the entire chromosome. In one study, oligonucle ...
Notes
Notes

... 95% of its germline genome during development, including the elimination of all transposon DNA, through an exaggerated process of genome rearrangement). Science, Vol. 324. no. 5929, pp. 935 – 938, 2009 ...
Engineering of robust synthetic gene networks with the help of
Engineering of robust synthetic gene networks with the help of

... Our focus: methods to understand transcription regulatory networks. The main players are Transcription Factors (TFs) that regulate the expression of 100-1000 of genes when necessary. ...
High-throughput reverse genetics: RNAi screens in
High-throughput reverse genetics: RNAi screens in

... What are the future prospects outside the worm? Wholegenome RNAi screening should be practical for the fly and plant genomes, with comparable yields of data. The vertebrate genome may not be as accessible to RNAi - its track record is still limited - but similar technologies such as morpholinooligon ...
Homeotic genes
Homeotic genes

... proteins is the transcription factor Distal-less (Dll). • Dll is required for the formation of legs in thoracic segments, and its expression is negatively regulated in abdominal segments by the actions of Ubx and AbdA they bind to the enhancer and suppress it. ...
Bioinformatics 3 V7 * Function Annotation, Gene Regulation
Bioinformatics 3 V7 * Function Annotation, Gene Regulation

... Left: Protein – DNA contacts involve many arginine (R) and lysine (K) residues ...
Gen660_Lecture1B_sequencing_2014
Gen660_Lecture1B_sequencing_2014

... Use a ‘super matrix’ of variable sequence characters 2. Construct many separate trees, one for each gene, and then compare Often construct a ‘super tree’ that is built from all single trees 3. Incorporate non-sequence characters like synteny, intron structure, etc. ...
Protein Synthesis
Protein Synthesis

... Alternative Splicing (of Exons) • How is it possible that there are millions of human antibodies when there are only about 30,000 genes? • Alternative splicing refers to the different ways the exons of a gene may be combined, producing different forms of proteins within the same gene-coding region ...
Gene Expression Microarray Analysis of Archival FFPE Samples
Gene Expression Microarray Analysis of Archival FFPE Samples

... samples represent the largest source of archival biological material available for large retrospective prognostic studies of human cancer, with over 400 million FFPE samples estimated in existence. Challenging samples such as degraded or FFPE tissues have remained difficult for gene expression micro ...
Word file (37 KB )
Word file (37 KB )

... The average number of reporters from these 78 classifiers is 238+/-23. Figure S4 presents the frequency of the original 231 genes and the union of other genes found in these 78 classifiers. We found that the vast majority of the original 231 reporter genes is commonly shared by the 78 classifiers. I ...
Determinants of  mRNA  localization University
Determinants of mRNA localization University

... polarized fashion. Within the cytoplasm, these determinants may specify a few, or many, compartments. Families of nearly identical proteins more often differ in their 3’.UTRs than in their coding sequences (e.g. actin). The mRNAs for the different isoforms may denote different compartments where eac ...
Protein Synthesis - Napa Valley College
Protein Synthesis - Napa Valley College

... information for making a protein from the nucleus to the cytosol  Transfer RNA (tRNA) – single strand, folds back on itself. Each tRNA carries one specific amino acid and brings it to the ribosome  Ribosomal RNA (rRNA) – globular structure, forms the catalytic part of ribosomes. Catalyzes the form ...
activator
activator

... Promoter Genes of operon ...
Walk-thru of CAGE exercise
Walk-thru of CAGE exercise

... – Etc What if we want to compare two experiments? ...
DNA level results in a phenotype of the patient
DNA level results in a phenotype of the patient

... many of the downstream splicing problems that gave rise to multiple DM phenotypes. Another study by (Ho et al. 2004) showed decreased expression of MBNL1 in cultured cells caused aberrant splicing in both the cardiac troponin T (cTNT) and insulin receptor (IR) genes, which is consistent with DM1 in ...
Operon: Lac trp Is the metabolic pathway anabolic or catabolic
Operon: Lac trp Is the metabolic pathway anabolic or catabolic

... 1. Under what circumstances would the lac operon be “on” versus “off”? The trp operon? The lac operon would be off when there is no lactose in the cell. The lac operon would be on when lactose is present and there is little or no glucose in the cell. However, the lac operon would be off (or operatin ...
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Short interspersed nuclear elements (SINEs)

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