DNA-free CRISPR-Cas9 genome engineering in
... The CRISPR-Cas9 system permits researchers to quickly edit genes for functional protein knockout in mammalian, fish and plant genomes, among others, and consequently has dramatically transformed biological research. The CRISPR-Cas9 system requires exogenous Cas9 nuclease to be delivered into the cel ...
... The CRISPR-Cas9 system permits researchers to quickly edit genes for functional protein knockout in mammalian, fish and plant genomes, among others, and consequently has dramatically transformed biological research. The CRISPR-Cas9 system requires exogenous Cas9 nuclease to be delivered into the cel ...
lac
... Regulation of the lac operon involves: Negative control Positive control Cis-acting DNA sequence Trans-acting protein factors ...
... Regulation of the lac operon involves: Negative control Positive control Cis-acting DNA sequence Trans-acting protein factors ...
Summary 121 Summary The Hox genes form a subset of the
... number of genes present in a cluster varies between animal species; the number of clusters in each species also varies. The Hox clusters are thought to have arisen by tandem duplication of a single gene, followed, in vertebrates, by duplication of the cluster itself. As a consequence, Hox genes occu ...
... number of genes present in a cluster varies between animal species; the number of clusters in each species also varies. The Hox clusters are thought to have arisen by tandem duplication of a single gene, followed, in vertebrates, by duplication of the cluster itself. As a consequence, Hox genes occu ...
RNA Processing
... – Is an important factor in determining the protein synthesis in a cell – Is determined in part by sequences in the leader and trailer regions ...
... – Is an important factor in determining the protein synthesis in a cell – Is determined in part by sequences in the leader and trailer regions ...
Journal of Biotechnology
... gaps revealed that most of the gaps (25 of 34) between scaffolded contigs were relatively small (3 ± 10 bp). They seem to be due to inverted repeats forming stable secondary structures which might result in no or poor amplification during the various PCR steps involved in library preparation and NGS ...
... gaps revealed that most of the gaps (25 of 34) between scaffolded contigs were relatively small (3 ± 10 bp). They seem to be due to inverted repeats forming stable secondary structures which might result in no or poor amplification during the various PCR steps involved in library preparation and NGS ...
Complete genome sequence of Roseophage vB_DshP
... E. coli N4, Roseophage DSS3P2 and vB_DshP-R1 were present, which suggested that they were strongly homologous. Based on the alignment of the DNA pol amino acid sequences, phage vB_DshP-R1 closely clustered with the four representative N4 Roseophages (~80% identity) described above (Figure 2). Analys ...
... E. coli N4, Roseophage DSS3P2 and vB_DshP-R1 were present, which suggested that they were strongly homologous. Based on the alignment of the DNA pol amino acid sequences, phage vB_DshP-R1 closely clustered with the four representative N4 Roseophages (~80% identity) described above (Figure 2). Analys ...
Complete genome sequence of Roseophage vB_DshP
... E. coli N4, Roseophage DSS3P2 and vB_DshP-R1 were present, which suggested that they were strongly homologous. Based on the alignment of the DNA pol amino acid sequences, phage vB_DshP-R1 closely clustered with the four representative N4 Roseophages (~80% identity) described above (Figure 2). Analys ...
... E. coli N4, Roseophage DSS3P2 and vB_DshP-R1 were present, which suggested that they were strongly homologous. Based on the alignment of the DNA pol amino acid sequences, phage vB_DshP-R1 closely clustered with the four representative N4 Roseophages (~80% identity) described above (Figure 2). Analys ...
Modulation of CTCF Insulator Function by
... CTCF plays diverse roles in the regulation of eukaryotic genes. A new study by Lefevre et al. in a recent issue of Molecular Cell reveals a novel mechanism in which noncoding RNA transcription and nucleosome repositioning evicts CTCF from a regulatory element to facilitate induction of a nearby gene ...
... CTCF plays diverse roles in the regulation of eukaryotic genes. A new study by Lefevre et al. in a recent issue of Molecular Cell reveals a novel mechanism in which noncoding RNA transcription and nucleosome repositioning evicts CTCF from a regulatory element to facilitate induction of a nearby gene ...
Structural organization of the malaria mosquito heterochromatin
... portion of the malaria mosquito Anopheles gambiae genome. Methods and Algorithms: Imunostaining of An. gambiae polytene chromosomes was performed using antibodies against Heterochromatin Protein 1 (HP1) and lamin Dm0 Drosophila melanogaster. Physical mapping was done by Cy3, Cy5 labeled PCR amplifie ...
... portion of the malaria mosquito Anopheles gambiae genome. Methods and Algorithms: Imunostaining of An. gambiae polytene chromosomes was performed using antibodies against Heterochromatin Protein 1 (HP1) and lamin Dm0 Drosophila melanogaster. Physical mapping was done by Cy3, Cy5 labeled PCR amplifie ...
Table 7. Summary statistics for the consensus gene set of Haliotis
... from the distinctive patterns of repeat element expansion between the two species and the increased genome size of H.discus hannai may be associated with the non-LTR elements (especially LINE/I) contribution, in parallel to the human genome[23]. Genes were predicted through three different algorithm ...
... from the distinctive patterns of repeat element expansion between the two species and the increased genome size of H.discus hannai may be associated with the non-LTR elements (especially LINE/I) contribution, in parallel to the human genome[23]. Genes were predicted through three different algorithm ...
The Non-LTR Retrotransposon Rex3 from the Fish Xiphophorus is
... and 17, depending on the copy). The stop codon that terminates the C-terminal-domain-encoding sequence is located at a distance of only 3 nt upstream of the first GAA repeat. This indicates that the 39 untranslated region (UTR) of Rex3 is extremely short. Small target sequence duplications of variou ...
... and 17, depending on the copy). The stop codon that terminates the C-terminal-domain-encoding sequence is located at a distance of only 3 nt upstream of the first GAA repeat. This indicates that the 39 untranslated region (UTR) of Rex3 is extremely short. Small target sequence duplications of variou ...
"What is a gene, in the post-ENCODE era?"
... It was the solution of the three-dimensional structure of DNA by Watson and Crick in 1953 (Watson and Crick 1953) that explained how DNA could function as the molecule of heredity. Base pairing explained how genetic information could be copied, and the existence of two strands explained how occasion ...
... It was the solution of the three-dimensional structure of DNA by Watson and Crick in 1953 (Watson and Crick 1953) that explained how DNA could function as the molecule of heredity. Base pairing explained how genetic information could be copied, and the existence of two strands explained how occasion ...
a real-time quantitative polymerase chain reaction protocol for symb
... isolation using a high-salt precipitation step involving the addition of 250 μL of a salt solution (0.8 m Na citrate, 1.2 m NaCl) before the addition of 250 μL of isopropanol. The RNA was eluted in 30 μL DEPC-treated water. After the aqueous phase was removed from each sample and processed for RNA ( ...
... isolation using a high-salt precipitation step involving the addition of 250 μL of a salt solution (0.8 m Na citrate, 1.2 m NaCl) before the addition of 250 μL of isopropanol. The RNA was eluted in 30 μL DEPC-treated water. After the aqueous phase was removed from each sample and processed for RNA ( ...
Functional gene groups are concentrated within chromosomes
... organization of co-functioning genes is found in prokaryotes, where genes, usually from the same functional family, are often arranged in operons (1,2). Genes in an operon reside consecutively along the genome and are governed by a common promoter. In contrast, most studied eukaryotes lack operons, ...
... organization of co-functioning genes is found in prokaryotes, where genes, usually from the same functional family, are often arranged in operons (1,2). Genes in an operon reside consecutively along the genome and are governed by a common promoter. In contrast, most studied eukaryotes lack operons, ...
What is a gene, post-ENCODE? History and updated definition
... It was the solution of the three-dimensional structure of DNA by Watson and Crick in 1953 (Watson and Crick 1953) that explained how DNA could function as the molecule of heredity. Base pairing explained how genetic information could be copied, and the existence of two strands explained how occasion ...
... It was the solution of the three-dimensional structure of DNA by Watson and Crick in 1953 (Watson and Crick 1953) that explained how DNA could function as the molecule of heredity. Base pairing explained how genetic information could be copied, and the existence of two strands explained how occasion ...
TNFα signals through specialized factories where responsive coding
... the approach correctly, is based on sequencing just ~80 clones and produces something like 30 "contacts". The high throughput sequencing approach in contrast has millions of reads, yet produces only a few fold more "contacts". Why? Does this mean that the number of true contacts is relatively small? ...
... the approach correctly, is based on sequencing just ~80 clones and produces something like 30 "contacts". The high throughput sequencing approach in contrast has millions of reads, yet produces only a few fold more "contacts". Why? Does this mean that the number of true contacts is relatively small? ...
Operon Comparison Chart
... the repressor to activate it), which turns the operon OFF (so no more tryptophan is made) ...
... the repressor to activate it), which turns the operon OFF (so no more tryptophan is made) ...
MAK, a computational tool kit for automated MITE
... elements and the anchored elements. In the automated anchor finding process, a BLASTN is carried out at very low stringency (with high E_value, lowest word size, high hitlist_size, low gapcosts and a specified organism) for shortest hits possible. All HSPs that match either end of the query sequence ...
... elements and the anchored elements. In the automated anchor finding process, a BLASTN is carried out at very low stringency (with high E_value, lowest word size, high hitlist_size, low gapcosts and a specified organism) for shortest hits possible. All HSPs that match either end of the query sequence ...
A conserved microRNA module exerts homeotic control over
... translation are expected to be moderate in this case and are not easily quantifiable, in particular if target gene expression is under feedback regulation by the protein products of these very genes16. Nevertheless, quantitative RT-PCR, a more sensitive means of studying steady-state transcript leve ...
... translation are expected to be moderate in this case and are not easily quantifiable, in particular if target gene expression is under feedback regulation by the protein products of these very genes16. Nevertheless, quantitative RT-PCR, a more sensitive means of studying steady-state transcript leve ...
El Proyecto Genoma Humano
... • BioProject is an administrative object (defined by goal, target, funding, collaboration) • Genome is a biological object defining an organism at molecular level • Genome assembly is a complex data structure that defines the structure, relative position (scaffold) and chromosome placement of DNA se ...
... • BioProject is an administrative object (defined by goal, target, funding, collaboration) • Genome is a biological object defining an organism at molecular level • Genome assembly is a complex data structure that defines the structure, relative position (scaffold) and chromosome placement of DNA se ...
What is a gene, post-ENCODE? History and updated
... It was the solution of the three-dimensional structure of DNA by Watson and Crick in 1953 (Watson and Crick 1953) that explained how DNA could function as the molecule of heredity. Base pairing explained how genetic information could be copied, and the existence of two strands explained how occasion ...
... It was the solution of the three-dimensional structure of DNA by Watson and Crick in 1953 (Watson and Crick 1953) that explained how DNA could function as the molecule of heredity. Base pairing explained how genetic information could be copied, and the existence of two strands explained how occasion ...
Click
... Transcript expression method MLE is not an accurate expression estim Parameters: Transcript relative abundance improves the robustness of expression ...
... Transcript expression method MLE is not an accurate expression estim Parameters: Transcript relative abundance improves the robustness of expression ...
View Full PDF
... control of differential gene expression [1]. Repressor proteins can affect transcription by multiple mechanisms. Repression may occur by directly targeting components of the RNA polymerase II (RNAPII) core transcription machinery to block the formation of the pre-initiation complex (PIC) [2–4]. The ...
... control of differential gene expression [1]. Repressor proteins can affect transcription by multiple mechanisms. Repression may occur by directly targeting components of the RNA polymerase II (RNAPII) core transcription machinery to block the formation of the pre-initiation complex (PIC) [2–4]. The ...
The nuclear envelope in genome organization, expression and
... its periphery 28. By suggesting that a gene-dense chromosome is internal and a gene-light chromosome is perinuclear, early studies relying on radial DNA positioning launched the quest to determine whether the position of a gene affects its function28. Since then, many questions have been answered bu ...
... its periphery 28. By suggesting that a gene-dense chromosome is internal and a gene-light chromosome is perinuclear, early studies relying on radial DNA positioning launched the quest to determine whether the position of a gene affects its function28. Since then, many questions have been answered bu ...
A Sunflower Helianthinin Gene Upstream Sequence
... competed by an excess of unlabeled EP-1 OSSsDr (compare lanes 3 and 4) and to a lesser extent by a similar excess of the helianthinin fragment D-404SHp (compare lanes 2 to 5 and 6) but not by fragments from vector DNA (compare lanes 2 and 3). These results indicate that the tested helianthinin and p ...
... competed by an excess of unlabeled EP-1 OSSsDr (compare lanes 3 and 4) and to a lesser extent by a similar excess of the helianthinin fragment D-404SHp (compare lanes 2 to 5 and 6) but not by fragments from vector DNA (compare lanes 2 and 3). These results indicate that the tested helianthinin and p ...