Dangerous exercise: lessons learned from dysregulated
... The usual balance between the pro- and anti-inflammatory exercise responses is occasionally upset, however, and when it is, disease may result. In the following, we review several examples of failed homeostatic inflammatory responses to exercise. Injury and overuse. Musculoskeletal sports injuries r ...
... The usual balance between the pro- and anti-inflammatory exercise responses is occasionally upset, however, and when it is, disease may result. In the following, we review several examples of failed homeostatic inflammatory responses to exercise. Injury and overuse. Musculoskeletal sports injuries r ...
Significance of the MHC Significance of the MHC
... MHC and immune responsiveness: In many cases, the ability of an inbred mouse strain to respond to a given antigen will depend on which alleles the strain carries at its MHC loci. The reason is that if an antigen cannot bind to an MHC molecule, it cannot be presented to T cells and therefore an immun ...
... MHC and immune responsiveness: In many cases, the ability of an inbred mouse strain to respond to a given antigen will depend on which alleles the strain carries at its MHC loci. The reason is that if an antigen cannot bind to an MHC molecule, it cannot be presented to T cells and therefore an immun ...
Protein splicing elements: inteins and exteins
... The protein sequences that flank the intein, and that are ligated to form the mature product, are defined as the exteins. Exteins are analogous to RNA exons. This nomenclature should replace that used in previous publications, where exteins were called 'external protein sequences' or 'EPSs'. We furt ...
... The protein sequences that flank the intein, and that are ligated to form the mature product, are defined as the exteins. Exteins are analogous to RNA exons. This nomenclature should replace that used in previous publications, where exteins were called 'external protein sequences' or 'EPSs'. We furt ...
Why does drug resistance readily evolve but vaccine resistance
... and life-history modifications [31,32]. Nevertheless, vaccine resistance is relatively rare, and when it does emerge, it tends to take longer than antimicrobial resistance (figure 1). It is well known that evolutionary trajectories are influenced by system-specific details. But there is a generality ...
... and life-history modifications [31,32]. Nevertheless, vaccine resistance is relatively rare, and when it does emerge, it tends to take longer than antimicrobial resistance (figure 1). It is well known that evolutionary trajectories are influenced by system-specific details. But there is a generality ...
ThermalAce™ DNA Polymerase
... those officers, employees and students of your institution who need access to perform the above-described internal research. You must inform each such officer, employee and student of the provisions of this license agreement and require them to agree to be bound by the provisions of this license agr ...
... those officers, employees and students of your institution who need access to perform the above-described internal research. You must inform each such officer, employee and student of the provisions of this license agreement and require them to agree to be bound by the provisions of this license agr ...
Cell-Mediated Immunity to Bordetella pertussis: Role of Thl
... A murine respiratory infection model was used to study the mechanism of protective immunity to BordeteUla pertussis. We found that nude mice, which are deficient in T cells, developed a persistent infection and failed to clear the bacteria after aerosol inoculation. In contrast, normal adult nonimmu ...
... A murine respiratory infection model was used to study the mechanism of protective immunity to BordeteUla pertussis. We found that nude mice, which are deficient in T cells, developed a persistent infection and failed to clear the bacteria after aerosol inoculation. In contrast, normal adult nonimmu ...
Detection of genetically modified cotton seeds using PCR
... products. Thermocycling was performed in a final volume of 25 µL (10.5 µL of water, 0.2 µM of each primer, 1 µL of genomic DNA and 12.5 µL of 2X iQ SYBR Green Supermix; Bio-Rad). The real-time PCR analysis was performed with the 5, 1 and 0.1% GMO dilution DNA standards along with the genomic DNA of ...
... products. Thermocycling was performed in a final volume of 25 µL (10.5 µL of water, 0.2 µM of each primer, 1 µL of genomic DNA and 12.5 µL of 2X iQ SYBR Green Supermix; Bio-Rad). The real-time PCR analysis was performed with the 5, 1 and 0.1% GMO dilution DNA standards along with the genomic DNA of ...
DpnII - Inv. PCR of miniMos for distribution
... protocol because we changed and optimized several parameters which in our hands improve the reliability of inverse PCR reactions. It is the protocol that we currently (December 2012) use in the lab. Use aerosol resistant tips for all steps!! Contamination is a real problem when doing two sequential ...
... protocol because we changed and optimized several parameters which in our hands improve the reliability of inverse PCR reactions. It is the protocol that we currently (December 2012) use in the lab. Use aerosol resistant tips for all steps!! Contamination is a real problem when doing two sequential ...
Plants` Epigenetic Secrets
... Although CHG methylation is symmetrical and thus could in principle use the same methylation maintenance pathway as CG methylation, it also relies on RNA-directed DNA methylation (not pictured below). In addition, this type of methylation is paired with methylation of lysine 9 on histone H3 (H3K9). ...
... Although CHG methylation is symmetrical and thus could in principle use the same methylation maintenance pathway as CG methylation, it also relies on RNA-directed DNA methylation (not pictured below). In addition, this type of methylation is paired with methylation of lysine 9 on histone H3 (H3K9). ...
PDF
... (Badenhorst et al., 2002; Badenhorst et al., 2005; Corona et al., 2007; Deuring et al., 2000; Hanai et al., 2008). In order to explore whether this was also true for ACF1 and to monitor its distribution at the single-cell level, we raised monoclonal antibodies and used them for both western blotting ...
... (Badenhorst et al., 2002; Badenhorst et al., 2005; Corona et al., 2007; Deuring et al., 2000; Hanai et al., 2008). In order to explore whether this was also true for ACF1 and to monitor its distribution at the single-cell level, we raised monoclonal antibodies and used them for both western blotting ...
MOLECULAR BIOLOGY AND BIOTECHNOLOGY
... o familiarize the students with the fundamental principles of Biotechnology, various developments in Biotechnology and its potential applications. Theory UNIT-I: History, scope and importance; DNA structure, function and metabolism. UNIT-II: DNA modifying enzymes and vectors; Methods of recombinant ...
... o familiarize the students with the fundamental principles of Biotechnology, various developments in Biotechnology and its potential applications. Theory UNIT-I: History, scope and importance; DNA structure, function and metabolism. UNIT-II: DNA modifying enzymes and vectors; Methods of recombinant ...
keyhole limpet haemocyanin, KLH
... when the antigen (protein) of interest is difficult or expensive to isolate in sufficient quantities, or when a single epitope of the protein has to be detected. Since a peptide molecule in itself is not immunogenic, it must be chemically coupled to a larger molecule (carrier) for successful immuniz ...
... when the antigen (protein) of interest is difficult or expensive to isolate in sufficient quantities, or when a single epitope of the protein has to be detected. Since a peptide molecule in itself is not immunogenic, it must be chemically coupled to a larger molecule (carrier) for successful immuniz ...
GIN Transposons: Genetic Elements Linking Retrotransposons and
... built including them and also sequences from Mdg1 retrotransposons, which were to be used as outgroups. The results are shown in figure 1, and the aligned sequences can be found in supplementary file 2 (Supplementary Material online). The detailed analysis of these new sequences totally changes our ...
... built including them and also sequences from Mdg1 retrotransposons, which were to be used as outgroups. The results are shown in figure 1, and the aligned sequences can be found in supplementary file 2 (Supplementary Material online). The detailed analysis of these new sequences totally changes our ...
Lectures prepared by Christine L. Case Chapter 8 Microbial Genetics
... event (less than 1%): very few cells in population are capable of exchanging and incorporating DNA. Three methods of prokaryotic gene transfer: Transformation (轉型) Conjugation (接合) ...
... event (less than 1%): very few cells in population are capable of exchanging and incorporating DNA. Three methods of prokaryotic gene transfer: Transformation (轉型) Conjugation (接合) ...
Program
... restrictions. In contrast, studies in mouse models can circumvent some of these limitations. Mouse models in immunology are a tool for understanding human diseases. Mice are the mainstay of in vivo immunological experimentation and in many respects they mirror human biology remarkably well. This con ...
... restrictions. In contrast, studies in mouse models can circumvent some of these limitations. Mouse models in immunology are a tool for understanding human diseases. Mice are the mainstay of in vivo immunological experimentation and in many respects they mirror human biology remarkably well. This con ...
Suppression of adaptive immunity to heterologous antigens during
... 5 years of age and causing 500 million clinical cases and up to 2.7 million deaths each year [1]. In addition to infectioninduced mortality, malaria is also associated with publichealth problems resulting from impairment of immune responses. Although this immunosuppression may have evolved as a mech ...
... 5 years of age and causing 500 million clinical cases and up to 2.7 million deaths each year [1]. In addition to infectioninduced mortality, malaria is also associated with publichealth problems resulting from impairment of immune responses. Although this immunosuppression may have evolved as a mech ...
... possible to use multiply biotinylated moieties (e.g., polybiotinylated enzymes) and avidin or streptavidin to create mixtures consisting of polymers of biotinylated moieties with avidin or streptavidin (3). These polymers could still have some free binding sites for biotin, thus becoming more-sensit ...
Peer-reviewed Article PDF
... bacteria S. thermophilus, which had just been sequenced, revealing an unusual CRISPR locus [4]. Although the CRISPR array was comparable to previously reported systems, it lacked some of the known Cas genes and instead contained unique Cas genes, including one encoding a large protein they predicted ...
... bacteria S. thermophilus, which had just been sequenced, revealing an unusual CRISPR locus [4]. Although the CRISPR array was comparable to previously reported systems, it lacked some of the known Cas genes and instead contained unique Cas genes, including one encoding a large protein they predicted ...
Introduction to Diagnostic and Therapeutic Monoclonal Antibodies
... Humoral immunity pertains to B-cells and their end products— memory B-cells and plasma cells (and their soluble secreted product, antibodies) which circulate in the humor, or extracellular fluids, such as plasma, the lymphatic system, and tissue fluids. B-cells die after about 6 months unless activa ...
... Humoral immunity pertains to B-cells and their end products— memory B-cells and plasma cells (and their soluble secreted product, antibodies) which circulate in the humor, or extracellular fluids, such as plasma, the lymphatic system, and tissue fluids. B-cells die after about 6 months unless activa ...
View PDF - OMICS International
... [1]. In the fall of 2001, B. anthracis spores were spread through letters mailed in the United States. Twenty two people were known to have been infected and five of those individuals died. A 92-page summary of evidence against the suspect, Bruce Ivins, was released by the FBI [2]. This most recent ...
... [1]. In the fall of 2001, B. anthracis spores were spread through letters mailed in the United States. Twenty two people were known to have been infected and five of those individuals died. A 92-page summary of evidence against the suspect, Bruce Ivins, was released by the FBI [2]. This most recent ...
Nucleic Acids: RNA and chemistry
... Note that we said there wasn’t much mRNA around at any given moment The amount synthesized is much greater because it has a much shorter lifetime than the others Ribonucleases act more avidly on it We need a mechanism for eliminating it because the cell wants to control concentrations of specific pr ...
... Note that we said there wasn’t much mRNA around at any given moment The amount synthesized is much greater because it has a much shorter lifetime than the others Ribonucleases act more avidly on it We need a mechanism for eliminating it because the cell wants to control concentrations of specific pr ...
A review of the human vs. porcine female genital tract
... challenge the health care systems worldwide. Animal models are essential for a deeper understanding of the diseases and the development of safe and protective vaccines. Currently a good predictive non-rodent model is needed for the study of genital chlamydia in women. The pig has become an increasin ...
... challenge the health care systems worldwide. Animal models are essential for a deeper understanding of the diseases and the development of safe and protective vaccines. Currently a good predictive non-rodent model is needed for the study of genital chlamydia in women. The pig has become an increasin ...
DNA vaccination
DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.