Mitochondria: an Unexpected Force in Innate Immunity
... signalling but they also take an active role in orchestrating the innate immune response to disruption of homeostasis. Furthermore, dysfunctional mitochondria can also act as activators of innate immunity, thus placing mitochondria squarely at the interface between cellular function and immune infla ...
... signalling but they also take an active role in orchestrating the innate immune response to disruption of homeostasis. Furthermore, dysfunctional mitochondria can also act as activators of innate immunity, thus placing mitochondria squarely at the interface between cellular function and immune infla ...
Chapter 13 - IARC Publications
... sufficient standardization to ensure comparability between methods and laboratories. In particular, quantitative measurements of cell receptor expression are often subject to considerable bias between methods (22–24). ...
... sufficient standardization to ensure comparability between methods and laboratories. In particular, quantitative measurements of cell receptor expression are often subject to considerable bias between methods (22–24). ...
Ends-out, or replacement, gene targeting in Drosophila
... to generate the extrachromosomal donor. To distinguish these possibilities we carried out a physical analysis of cutting at the I-SceI sites. Second and third instar larvae were heat shocked at 38°C for 1 h, and genomic DNA was prepared from samples of these larvae at various times after the heat sh ...
... to generate the extrachromosomal donor. To distinguish these possibilities we carried out a physical analysis of cutting at the I-SceI sites. Second and third instar larvae were heat shocked at 38°C for 1 h, and genomic DNA was prepared from samples of these larvae at various times after the heat sh ...
Genotyping BoLA-DRB3 alleles in Brazilian Dairy Gir cattle (Bos
... Avenue Pádua Dias 11, 13418-900 Piracicaba, SP, Brazil. Tel.: + 55 19 32494434; Fax: + 55 19 34294284; E-mail: [email protected] ...
... Avenue Pádua Dias 11, 13418-900 Piracicaba, SP, Brazil. Tel.: + 55 19 32494434; Fax: + 55 19 34294284; E-mail: [email protected] ...
Gene expression profile predicting the response to anti
... Microarray technology, a high-throughput genomic technology, can be used to identify predictive gene profiles in order to elucidate the complex networks and interactions in pathogenic processes and disease development [19]. Some researchers use the multiple gene microarray technology to identify gen ...
... Microarray technology, a high-throughput genomic technology, can be used to identify predictive gene profiles in order to elucidate the complex networks and interactions in pathogenic processes and disease development [19]. Some researchers use the multiple gene microarray technology to identify gen ...
functional analysis of chromatin assembly genes in tetrahymena
... would also like to acknowledge the contributions of the Natural Sciences and Engineering Research Council of Canada (NSERC) for funding this opportunity. This journey turned out to a memorable experience due to the laughter, stories, support and assistance that I was able to have with my fellow coll ...
... would also like to acknowledge the contributions of the Natural Sciences and Engineering Research Council of Canada (NSERC) for funding this opportunity. This journey turned out to a memorable experience due to the laughter, stories, support and assistance that I was able to have with my fellow coll ...
WHIP2015 book - Marine Biological Laboratory
... LEISHMANIA -‐-‐-‐ CHAIRs Phil Scott & Michael Grigg 8:30 Susmita Ghosh -‐-‐-‐ Leishmania and/or Host: Who drives the Immune Response during Indian Leishmaniasis? ...
... LEISHMANIA -‐-‐-‐ CHAIRs Phil Scott & Michael Grigg 8:30 Susmita Ghosh -‐-‐-‐ Leishmania and/or Host: Who drives the Immune Response during Indian Leishmaniasis? ...
Eliminating latent tuberculosis - Institute of Infectious Disease and
... Modern technologies, especially nucleic acid amplification, provide the opportunity to advance our detailed understanding of the status of bacteria present in tissue samples. Two interesting studies have demonstrated the presence of M. tuberculosis DNA outside both cells and granulomas in latently i ...
... Modern technologies, especially nucleic acid amplification, provide the opportunity to advance our detailed understanding of the status of bacteria present in tissue samples. Two interesting studies have demonstrated the presence of M. tuberculosis DNA outside both cells and granulomas in latently i ...
Protein aggregation complicates the development of baculovirus-expressed African horsesickness
... AHSV strains, particularly serotypes 1 and 6, in the vaccine-packaging section as a result of accidental breakages of bottles of freeze-dried vaccine. It is important, however, to stress that these infections occurred under specific circumstances with known encephalitogenic strains and there is no r ...
... AHSV strains, particularly serotypes 1 and 6, in the vaccine-packaging section as a result of accidental breakages of bottles of freeze-dried vaccine. It is important, however, to stress that these infections occurred under specific circumstances with known encephalitogenic strains and there is no r ...
T-cell intrinsic expression of MyD88 is required for sustained
... GP33–41 peptide in vitro (Fig. 1b). A markedly reduced response was also observed for NP396–404-specific CD8+ T cells and GP61–80-specific CD4+ T cells (Fig. 1b). The impaired T-cell response in MyD882/2 mice was evident by 6 days p.i. and became even more pronounced with time, eventually resulting ...
... GP33–41 peptide in vitro (Fig. 1b). A markedly reduced response was also observed for NP396–404-specific CD8+ T cells and GP61–80-specific CD4+ T cells (Fig. 1b). The impaired T-cell response in MyD882/2 mice was evident by 6 days p.i. and became even more pronounced with time, eventually resulting ...
Immunology, Nutrition, and the Athlete, Part II
... of need. Because of this, skeletal muscle and the glutamine it produces are thought to play a role in directly influencing the immune system. Decreased levels of plasma glutamine resulting from muscular overuse are thought to affect the function of lymphocytes.2 The “glutamine hypothesis” suggests ...
... of need. Because of this, skeletal muscle and the glutamine it produces are thought to play a role in directly influencing the immune system. Decreased levels of plasma glutamine resulting from muscular overuse are thought to affect the function of lymphocytes.2 The “glutamine hypothesis” suggests ...
FEMS Microbiology Letters
... G, similar to that in the comA null mutant (data not shown), indicating that the unphosphorylated form of ComA does not appear to have a role in the regulation of srfA. All of these results indicate that the majority of srfA expression during growth in DSM-G, in particular at late-growth phase, does ...
... G, similar to that in the comA null mutant (data not shown), indicating that the unphosphorylated form of ComA does not appear to have a role in the regulation of srfA. All of these results indicate that the majority of srfA expression during growth in DSM-G, in particular at late-growth phase, does ...
The Effects of varying pH on Plasmid Transfer
... integration is a very simple way to mobilize other genetic material. The F plasmid of Escherichia coli is an example of an episome. The F plasmid is not only conjugative but it can also integrate itself into the recipient’s chromosome. Strains of bacteria that possess an integrated F plasmid are cal ...
... integration is a very simple way to mobilize other genetic material. The F plasmid of Escherichia coli is an example of an episome. The F plasmid is not only conjugative but it can also integrate itself into the recipient’s chromosome. Strains of bacteria that possess an integrated F plasmid are cal ...
The energetic basis of the DNA double helix: a
... increase from the very beginning of heating, namely in the temperature range from 0 to 45◦ C, over which the duplex is generally regarded as still being fully folded. The observed rise in the heat capacity of the duplex on heating might result from increased fraying of its ends, in which case one wo ...
... increase from the very beginning of heating, namely in the temperature range from 0 to 45◦ C, over which the duplex is generally regarded as still being fully folded. The observed rise in the heat capacity of the duplex on heating might result from increased fraying of its ends, in which case one wo ...
Slide 1
... •Multiple alignment is an extension of pairwise alignment where multiple sequences are aligned •This alignment provides insights not possible in pairwise alignments, such as •Conserved sequence patterns •Conserved and functionally critical amino acid residues •Prerequisite for phylogenetic analyses ...
... •Multiple alignment is an extension of pairwise alignment where multiple sequences are aligned •This alignment provides insights not possible in pairwise alignments, such as •Conserved sequence patterns •Conserved and functionally critical amino acid residues •Prerequisite for phylogenetic analyses ...
Cytokine responses in human Lyme borreliosis
... midgut tissue but the proteins were not necessary for infection, dissemination or pathogenesis in mice (Yang et al. 2004). In contrast, OspC is important for infection shown by a OspC-deficient B. burgdorferi s. l. inability to infect mice (Grimm et al. 2004). The heterogeneity of the different prot ...
... midgut tissue but the proteins were not necessary for infection, dissemination or pathogenesis in mice (Yang et al. 2004). In contrast, OspC is important for infection shown by a OspC-deficient B. burgdorferi s. l. inability to infect mice (Grimm et al. 2004). The heterogeneity of the different prot ...
- Journal of Allergy and Clinical Immunology
... hygiene hypothesis is the skewing of the TH1/TH2 balance away from allergy-promoting TH2 cells toward TH1 cells.1 The link between the TH1/TH2 balance and allergic diseases is mediated in part by IgE: TH2 cells, by secreting IL-4 and IL-13, promote immunoglobulin class-switch recombination to IgE (f ...
... hygiene hypothesis is the skewing of the TH1/TH2 balance away from allergy-promoting TH2 cells toward TH1 cells.1 The link between the TH1/TH2 balance and allergic diseases is mediated in part by IgE: TH2 cells, by secreting IL-4 and IL-13, promote immunoglobulin class-switch recombination to IgE (f ...
Respiratory and systemic humoral and cellular immune responses
... The level of heterosubtypic immunity (Het-I) and the immune mechanisms stimulated by a heterosubtypic influenza virus infection were investigated in pigs. Pigs are natural hosts for influenza virus and, like humans, they host both subtypes H1N1 and H3N2. Marked Het-I was observed when pigs were infe ...
... The level of heterosubtypic immunity (Het-I) and the immune mechanisms stimulated by a heterosubtypic influenza virus infection were investigated in pigs. Pigs are natural hosts for influenza virus and, like humans, they host both subtypes H1N1 and H3N2. Marked Het-I was observed when pigs were infe ...
Factors affecting the antigen-antibody reaction (PDF
... Affinity maturation is the progressive increase in the equilibrium constant of antibodies produced during the immune response. Typically, early antibodies have equilibrium constants of around 105-106; those produced after a few months show 100-fold greater affinities24. The structural basis of affin ...
... Affinity maturation is the progressive increase in the equilibrium constant of antibodies produced during the immune response. Typically, early antibodies have equilibrium constants of around 105-106; those produced after a few months show 100-fold greater affinities24. The structural basis of affin ...
PPT - Altogen Biosystems
... Altogen Biosystems offers the MG-63 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The MG-63 Transfection Reagent is an advanced formulation of a lipid based reagent, and it has been developed to provide high transfection efficiency with the MG-63 cell line. ...
... Altogen Biosystems offers the MG-63 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits. The MG-63 Transfection Reagent is an advanced formulation of a lipid based reagent, and it has been developed to provide high transfection efficiency with the MG-63 cell line. ...
Oxidative Stress in Cardiovascular Inflammation: Its Involvement in
... implicated in loading of immunogenic peptides to major histocompatibility class (MHC) I and II molecules [41]. Besides being expressed in cells under physiological conditions, HSPs increase in response to many environmental stresses, including oxidative stress [42]. Under stress conditions, HSPs are ...
... implicated in loading of immunogenic peptides to major histocompatibility class (MHC) I and II molecules [41]. Besides being expressed in cells under physiological conditions, HSPs increase in response to many environmental stresses, including oxidative stress [42]. Under stress conditions, HSPs are ...
Directed Evolution of Polymerases To Accept Nucleotides with
... primers are, however, tagged with DNA sequences that contain multiple P’s. The small amounts of chimeric primers are rapidly consumed in the initial rounds of PCR, requiring the external primers to later “carry” the amplification. Because the external primers must be present at high concentrations fo ...
... primers are, however, tagged with DNA sequences that contain multiple P’s. The small amounts of chimeric primers are rapidly consumed in the initial rounds of PCR, requiring the external primers to later “carry” the amplification. Because the external primers must be present at high concentrations fo ...
Genetic background affects susceptibility in nonfatal pneumococcal bronchopneumonia J.A. Preston , K.W. Beagley
... beginning. Preliminary data suggest that neutrophil influx and increased tumour necrosis factor (TNF)-a in bronchoalveolar lavage (BAL) fluid and lung tissue are important in pneumococcal clearance [17, 18]. There is increasing evidence that links the outcome of infectious disease to variations in t ...
... beginning. Preliminary data suggest that neutrophil influx and increased tumour necrosis factor (TNF)-a in bronchoalveolar lavage (BAL) fluid and lung tissue are important in pneumococcal clearance [17, 18]. There is increasing evidence that links the outcome of infectious disease to variations in t ...
Expression of Dengue virus envelope glycoproteins using a
... epithelial cells (18). The virus amplification happens in other mosquito’s tissues and cells, which results in the distribution of the virus through the hemocele (the mosquito circulatory system). After the amplification of the virus, it will infect the epithelial cells of the salivary gland and th ...
... epithelial cells (18). The virus amplification happens in other mosquito’s tissues and cells, which results in the distribution of the virus through the hemocele (the mosquito circulatory system). After the amplification of the virus, it will infect the epithelial cells of the salivary gland and th ...
DNA vaccination
DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.