Separation of DNA Restriction Fragments by Ion
... (D) The fact that the two ion-exchange materials are made from the same basic support yet have differences should make possible comparisons between the relative importance ofthe basic support versus the charged substituents: Mono Q is a strong ion exchanger with a high density of quaternary amino gr ...
... (D) The fact that the two ion-exchange materials are made from the same basic support yet have differences should make possible comparisons between the relative importance ofthe basic support versus the charged substituents: Mono Q is a strong ion exchanger with a high density of quaternary amino gr ...
The plant genome`s methylation status and response to stress
... Differences in DNA methylation within a single genotype can also contribute to heritable trait differences that can be selected. In maize, alleles of B1 and the pericarp color gene P1, another transcriptional regulator of the flavonoid biosynthetic pathway, can have identical sequences but different ...
... Differences in DNA methylation within a single genotype can also contribute to heritable trait differences that can be selected. In maize, alleles of B1 and the pericarp color gene P1, another transcriptional regulator of the flavonoid biosynthetic pathway, can have identical sequences but different ...
Amplification of 16S rRNA Genes from Frankia Strains in Root
... (14, 15), Dryas spp. (23), Alnus spp., and Myrica spp. (17, 26, 27). With some exceptions, most procedures have required the use of relatively large quantities of nodule tissue for DNA isolation, and PCR amplification has usually been limited to short segments of 16S ribosomal DNA (rDNA). Two contin ...
... (14, 15), Dryas spp. (23), Alnus spp., and Myrica spp. (17, 26, 27). With some exceptions, most procedures have required the use of relatively large quantities of nodule tissue for DNA isolation, and PCR amplification has usually been limited to short segments of 16S ribosomal DNA (rDNA). Two contin ...
Discovery and characterization of chromatin states for Please share
... The first group of states, states 1–11, all had high enrichment for promoter regions: 40%– 89% of each state was within 2kb of a RefSeq transcription start site (TSS), compared with 2.7% genome-wide (P<10−200, for all states). These states accounted for 59% of all RefSeq TSS while covering only 1.3% ...
... The first group of states, states 1–11, all had high enrichment for promoter regions: 40%– 89% of each state was within 2kb of a RefSeq transcription start site (TSS), compared with 2.7% genome-wide (P<10−200, for all states). These states accounted for 59% of all RefSeq TSS while covering only 1.3% ...
Use of a novel cassette to label phenotypically a cryptic plasmid of
... insertion/deletion derivatives. We have identified the replication region as well as separate regions required for segregational and structural stability. The segregational mechanism is very efficient since it allows no detectable loss despite the fact that bacteria carrying the plasmid have a great ...
... insertion/deletion derivatives. We have identified the replication region as well as separate regions required for segregational and structural stability. The segregational mechanism is very efficient since it allows no detectable loss despite the fact that bacteria carrying the plasmid have a great ...
Maintenance of DNA Methylation during the Arabidopsis Life Cycle
... detected transcripts from active postzygotic transcription of the FIS2 maternal allele in the endosperm. A putative general paternal silencing has been observed for reporters of endospermexpressed genes for up to 4 DAP (Vielle-Calzada et al., 2000). It could be hypothesized that such a general mecha ...
... detected transcripts from active postzygotic transcription of the FIS2 maternal allele in the endosperm. A putative general paternal silencing has been observed for reporters of endospermexpressed genes for up to 4 DAP (Vielle-Calzada et al., 2000). It could be hypothesized that such a general mecha ...
Molecular insights into mitochondrial transcription and its
... triphosphate (ATP), through the process of oxidative phosphorylation. The mitochondrion contains its own genome, a small circular DNA molecule (mtDNA), encoding essential subunits of the oxidative phosphorylation system. Initiation of mitochondrial transcription involves three proteins, the mitochon ...
... triphosphate (ATP), through the process of oxidative phosphorylation. The mitochondrion contains its own genome, a small circular DNA molecule (mtDNA), encoding essential subunits of the oxidative phosphorylation system. Initiation of mitochondrial transcription involves three proteins, the mitochon ...
Demarcation of coding and non-coding regions of DNA using linear
... Deoxyribonucleic Acid (DNA) strand carries genetic information in the cell. A strand of DNA consists of nitrogenous molecules called nucleotides. Nucleotides triplets, or the codons, code for amino acids. There are two distinct regions in DNA, the gene and the intergenic DNA, or the junk DNA. Two re ...
... Deoxyribonucleic Acid (DNA) strand carries genetic information in the cell. A strand of DNA consists of nitrogenous molecules called nucleotides. Nucleotides triplets, or the codons, code for amino acids. There are two distinct regions in DNA, the gene and the intergenic DNA, or the junk DNA. Two re ...
Aberrant DNA methylation in cancer: potential clinical
... are comparatively rich in CpG nucleotides and are nearly always free of methylation. These CpG islands are frequently located within the promoter regions of human genes, and methylation within the islands has been shown to be associated with transcriptional inactivation of the corresponding gene. Al ...
... are comparatively rich in CpG nucleotides and are nearly always free of methylation. These CpG islands are frequently located within the promoter regions of human genes, and methylation within the islands has been shown to be associated with transcriptional inactivation of the corresponding gene. Al ...
CH Zinc Fingers As DNA Binding Domains
... to establish the binding to the target DNA.^^ The other classes, that is the triple, multiple-adjacent and separated-paired C2H2 fingers, bind to the specific DNA sequence without the aid of other domains. Both the triple C2H2 and the multiple-adjacent C2H2 zinc fingers bind to the cognate DNA at th ...
... to establish the binding to the target DNA.^^ The other classes, that is the triple, multiple-adjacent and separated-paired C2H2 fingers, bind to the specific DNA sequence without the aid of other domains. Both the triple C2H2 and the multiple-adjacent C2H2 zinc fingers bind to the cognate DNA at th ...
Analysis of Drosophila Species Genome Size and Satellite DNA
... 2000; Bensasson et al. 2001; Petrov 2001). Genome size in these examples clearly does not correlate with the number of genes found in each genome or with the complexity of the organism. It appears, instead, that the vast differences in genome size are a result of repetitive DNA sequences that litter ...
... 2000; Bensasson et al. 2001; Petrov 2001). Genome size in these examples clearly does not correlate with the number of genes found in each genome or with the complexity of the organism. It appears, instead, that the vast differences in genome size are a result of repetitive DNA sequences that litter ...
DNA Three Way Junction Core Decorated with Amino Acids
... functionalized by protein-like residues (imidazole, alcohol and carboxylic acid) at unpaired positions at the core is described. One 50 -C(S)-propargyl-thymidine nucleotide was specifically incorporated on each strand to react through a post synthetic CuACC reaction with either protected imidazolyl- ...
... functionalized by protein-like residues (imidazole, alcohol and carboxylic acid) at unpaired positions at the core is described. One 50 -C(S)-propargyl-thymidine nucleotide was specifically incorporated on each strand to react through a post synthetic CuACC reaction with either protected imidazolyl- ...
Ancient DNA Laboratory Guidelines
... room. This should be done every day that the aDNA Laboratory is used, even if projects require the use of the aDNA Laboratory over several days. Publishable aDNA Laboratory Results “Ancient” DNA studies over the past few years have almost become routine. However, risks of contamination are also now ...
... room. This should be done every day that the aDNA Laboratory is used, even if projects require the use of the aDNA Laboratory over several days. Publishable aDNA Laboratory Results “Ancient” DNA studies over the past few years have almost become routine. However, risks of contamination are also now ...
Point mutations in BCL6 DNA-binding domain reveal distinct
... We show here by point mutation analysis that the six ZFs of BCL6 are not equivalent in DNA-binding activity. Similar findings were reported for the POK proteins ZF5 and Kaiso [12 and 13]. The described experiments differed from our work though, as the ZF region of these two proteins was not examined ...
... We show here by point mutation analysis that the six ZFs of BCL6 are not equivalent in DNA-binding activity. Similar findings were reported for the POK proteins ZF5 and Kaiso [12 and 13]. The described experiments differed from our work though, as the ZF region of these two proteins was not examined ...
unit-2 genetics of prokaryotes and eukaryotic
... chromosome or for finding out distances of these genes from centromere, this technique is called chromosome mapping. If linkage groups are already established in an organism, trisomics can be effectively used for assigning these linkage groups to specific chromosomes. Since the segregation ratio for ...
... chromosome or for finding out distances of these genes from centromere, this technique is called chromosome mapping. If linkage groups are already established in an organism, trisomics can be effectively used for assigning these linkage groups to specific chromosomes. Since the segregation ratio for ...
Investigating the DNA-Binding Interactions of Small
... and other disorders. In this dissertation, the DNA-binding interactions of small organic molecules were investigated. We developed a highly sensitive methodology to determine the DNA-binding mode of small molecules unambiguously unlike other conventional techniques. Furthermore, we established struc ...
... and other disorders. In this dissertation, the DNA-binding interactions of small organic molecules were investigated. We developed a highly sensitive methodology to determine the DNA-binding mode of small molecules unambiguously unlike other conventional techniques. Furthermore, we established struc ...
Replication of plasmids with the p15A origin in Shewanella
... this possibility, we similarly replaced the 383-bp DraI-NcoI fragment of the Cmr gene of pACYC184 with a 224-bp DraINcoI fragment of an MR-1 genomic ORF that complements a previously isolated mutant deficient in the reduction of Fe(III), nitrate and fumarate (Myers and Myers 1993b). After purificati ...
... this possibility, we similarly replaced the 383-bp DraI-NcoI fragment of the Cmr gene of pACYC184 with a 224-bp DraINcoI fragment of an MR-1 genomic ORF that complements a previously isolated mutant deficient in the reduction of Fe(III), nitrate and fumarate (Myers and Myers 1993b). After purificati ...
Laboratory manual for the diagnosis of whooping cough caused by... pertussis/ Bordetella parapertussis
... BvgA/S genes, the vag are not expressed. Instead, a set of other genes called vir-repressed genes (vrg) are expressed. The function of the proteins encoded by the vrg and their role in the pathogenicity of B. pertussis are not yet known. In that case Bordetella species are called phase IV Bordetella ...
... BvgA/S genes, the vag are not expressed. Instead, a set of other genes called vir-repressed genes (vrg) are expressed. The function of the proteins encoded by the vrg and their role in the pathogenicity of B. pertussis are not yet known. In that case Bordetella species are called phase IV Bordetella ...
De Novo Nonsense Mutations in KAT6A, a Lysine Acetyl
... Histone-modifying enzymes play key roles in transcriptional regulation and control major cellular processes such as the cell cycle,1,2 stem cell maintenance, and differentiation.3,4 These enzymes function within multisubunit protein complexes that target acetyltransferases and deacetylases to specif ...
... Histone-modifying enzymes play key roles in transcriptional regulation and control major cellular processes such as the cell cycle,1,2 stem cell maintenance, and differentiation.3,4 These enzymes function within multisubunit protein complexes that target acetyltransferases and deacetylases to specif ...
Equilibrium Statistics of Channel-confined DNA
... of different experimental techniques [31, 33], showing that it is a good model of DNA, except when subjected to very high forces [34, 35], or in the presence of positive polyvalent ions [36]. The contour length can be directly computed from the lateral distance between base pairs as L = (Number of b ...
... of different experimental techniques [31, 33], showing that it is a good model of DNA, except when subjected to very high forces [34, 35], or in the presence of positive polyvalent ions [36]. The contour length can be directly computed from the lateral distance between base pairs as L = (Number of b ...
DNA phosphorothioation inStreptomyces lividans: mutational
... Most of the commonly found structural changes in DNA are due to methylation of selected bases. In some viral DNAs, certain bases may be hydroxymethylated or gluco- ...
... Most of the commonly found structural changes in DNA are due to methylation of selected bases. In some viral DNAs, certain bases may be hydroxymethylated or gluco- ...
Part III: Laboratory – Electrophoresis
... The laboratory is organized by part. Each part has notes for the instructor (when necessary), preparation instructions, and the experimental protocol for the lab. A separate Results and Discussion is also provided with some additional suggested analysis. The lab is set-up to have students work in pa ...
... The laboratory is organized by part. Each part has notes for the instructor (when necessary), preparation instructions, and the experimental protocol for the lab. A separate Results and Discussion is also provided with some additional suggested analysis. The lab is set-up to have students work in pa ...
Origins of DNA Replication - DNA Replication and Human Disease
... and origin recognition proteins, as well as differences in the mechanism by which ori-auxiliary components function. 2. The genetic origin is coincident with the functional origin. The functional origin is the site where DNA synthesis actually begins. It has been mapped to within +300 bp of the gene ...
... and origin recognition proteins, as well as differences in the mechanism by which ori-auxiliary components function. 2. The genetic origin is coincident with the functional origin. The functional origin is the site where DNA synthesis actually begins. It has been mapped to within +300 bp of the gene ...
Nucleosome
A nucleosome is a basic unit of DNA packaging in eukaryotes, consisting of a segment of DNA wound in sequence around eight histone protein cores. This structure is often compared to thread wrapped around a spool.Nucleosomes form the fundamental repeating units of eukaryotic chromatin, which is used to pack the large eukaryotic genomes into the nucleus while still ensuring appropriate access to it (in mammalian cells approximately 2 m of linear DNA have to be packed into a nucleus of roughly 10 µm diameter). Nucleosomes are folded through a series of successively higher order structures to eventually form a chromosome; this both compacts DNA and creates an added layer of regulatory control, which ensures correct gene expression. Nucleosomes are thought to carry epigenetically inherited information in the form of covalent modifications of their core histones.Nucleosomes were observed as particles in the electron microscope by Don and Ada Olins and their existence and structure (as histone octamers surrounded by approximately 200 base pairs of DNA) were proposed by Roger Kornberg. The role of the nucleosome as a general gene repressor was demonstrated by Lorch et al. in vitro and by Han and Grunstein in vivo.The nucleosome core particle consists of approximately 147 base pairs of DNA wrapped in 1.67 left-handed superhelical turns around a histone octamer consisting of 2 copies each of the core histones H2A, H2B, H3, and H4. Core particles are connected by stretches of ""linker DNA"", which can be up to about 80 bp long. Technically, a nucleosome is defined as the core particle plus one of these linker regions; however the word is often synonymous with the core particle. Genome-wide nucleosome positioning maps are now available for many model organisms including mouse liver and brain.Linker histones such as H1 and its isoforms are involved in chromatin compaction and sit at the base of the nucleosome near the DNA entry and exit binding to the linker region of the DNA. Non-condensed nucleosomes without the linker histone resemble ""beads on a string of DNA"" under an electron microscope.In contrast to most eukaryotic cells, mature sperm cells largely use protamines to package their genomic DNA, most likely to achieve an even higher packaging ratio. Histone equivalents and a simplified chromatin structure have also been found in Archea, suggesting that eukaryotes are not the only organisms that use nucleosomes.