Inhibition of RNA Synthesis by Anthracycline Analogs
... and inactivate its template function essential for nucleic acid synthesis. Recently, several structural analogs of these antineoplastic agents have been synthesized and some of them were shown to be considerably more effective against experimental tumors than the parent compounds. The majority of re ...
... and inactivate its template function essential for nucleic acid synthesis. Recently, several structural analogs of these antineoplastic agents have been synthesized and some of them were shown to be considerably more effective against experimental tumors than the parent compounds. The majority of re ...
Molecular Biology and Applied Genetics
... health institutions. It can also be used in teaching any introductory course on medical Applied Genetics and Molecular Biology and as a reference material. This lecture note is specifically designed for medical laboratory technologists, and includes only those areas of molecular cell biology and App ...
... health institutions. It can also be used in teaching any introductory course on medical Applied Genetics and Molecular Biology and as a reference material. This lecture note is specifically designed for medical laboratory technologists, and includes only those areas of molecular cell biology and App ...
KingFisher Pure DNA Plant Kit
... of highly pure DNA from plant material using KingFisher magnetic particle processors. If the homogenization of the starting material is excluded, the approximate processing time is 40 minutes for the purification of 96 samples on the KingFisher Flex and 12 samples on the KingFisher Duo when the samp ...
... of highly pure DNA from plant material using KingFisher magnetic particle processors. If the homogenization of the starting material is excluded, the approximate processing time is 40 minutes for the purification of 96 samples on the KingFisher Flex and 12 samples on the KingFisher Duo when the samp ...
1 bio. hints of aipmt mt – viii
... generation was of hybrid or intermediate type only. In lac operon, the repressor is synthesised all the time. 34. Ans. (b) Hint: The cell envelope consists of outermost glycocalyx, followed by the cell wall and then the plasma membrane. Glycocalyx can be a loose sheath called the slime layer in some ...
... generation was of hybrid or intermediate type only. In lac operon, the repressor is synthesised all the time. 34. Ans. (b) Hint: The cell envelope consists of outermost glycocalyx, followed by the cell wall and then the plasma membrane. Glycocalyx can be a loose sheath called the slime layer in some ...
File
... present in the extract. In so doing, they found that the selective removal of DNA with deoxyribonuclease was the only treatment that resulted in the transforming activity being lost. In addition, they isolated pure DNA from heat-killed S cells and showed that it alone was capable of transforming R c ...
... present in the extract. In so doing, they found that the selective removal of DNA with deoxyribonuclease was the only treatment that resulted in the transforming activity being lost. In addition, they isolated pure DNA from heat-killed S cells and showed that it alone was capable of transforming R c ...
Harvey ras (H-ras) Point Mutations Are Induced by 4
... DMBA, and the promoter, TPA (12, 13). Continuous exposure of squamous cells to DMBA and TPA induced H-ras mutations on chromosome 7 in greater than 90% of mice (14). H-ras appeared to be activated by specific mutations which can be affected by the initiating carcinogen (15). Since tumors do not deve ...
... DMBA, and the promoter, TPA (12, 13). Continuous exposure of squamous cells to DMBA and TPA induced H-ras mutations on chromosome 7 in greater than 90% of mice (14). H-ras appeared to be activated by specific mutations which can be affected by the initiating carcinogen (15). Since tumors do not deve ...
Molecular mechanics of the interactions of spermine with DNA: DNA
... implies stabilization of double-stranded over single-stranded forms. It was found recently that polyamines also stabilize both the left-handed Z conformation (10,11) and the right-handed A conformation (12) of nucleic acids. Stabilization of specific DNA ...
... implies stabilization of double-stranded over single-stranded forms. It was found recently that polyamines also stabilize both the left-handed Z conformation (10,11) and the right-handed A conformation (12) of nucleic acids. Stabilization of specific DNA ...
Localization and structural analysis of the ribosomal RNA operons of
... post-transcriptional levels. The cellular content of specific mRNA and protein components unique to photosynthetic growth are either not detectable or present at extremely low levels in aerobically growing cells (3). Because of the apparent translational coupling between the synthesis of specific IC ...
... post-transcriptional levels. The cellular content of specific mRNA and protein components unique to photosynthetic growth are either not detectable or present at extremely low levels in aerobically growing cells (3). Because of the apparent translational coupling between the synthesis of specific IC ...
Gene Detection Systems Catalog
... your name from the dropdown USER name field. If you are a 'new' user, select NEW and enter your name, email address and telephone number. This information will be stored and your name will appear the next time you login. Order Changes and Cancellation All catalog product orders are routinely shipped ...
... your name from the dropdown USER name field. If you are a 'new' user, select NEW and enter your name, email address and telephone number. This information will be stored and your name will appear the next time you login. Order Changes and Cancellation All catalog product orders are routinely shipped ...
Isolation, Characterization, and Annotation: The Search for Novel
... to the host, penetrates the cell membrane and injects its DNA. The genetic information can then follow two potential paths. In the lysogenic cycle, the DNA is incorporated into the host genome as a prophage and remains a part of the host’s genome as long as conditions remain stable for the prophage. ...
... to the host, penetrates the cell membrane and injects its DNA. The genetic information can then follow two potential paths. In the lysogenic cycle, the DNA is incorporated into the host genome as a prophage and remains a part of the host’s genome as long as conditions remain stable for the prophage. ...
A Novel CpG Island Set Identifies Tissue-Specific
... sequences called ‘‘CpG islands’’ (CGIs). These sequences are found at over half of all human genes and can exist in either the active or silent state depending on the presence or absence of methyl groups on the DNA. We devised a method for purifying all CGIs and showed that, unexpectedly, only half ...
... sequences called ‘‘CpG islands’’ (CGIs). These sequences are found at over half of all human genes and can exist in either the active or silent state depending on the presence or absence of methyl groups on the DNA. We devised a method for purifying all CGIs and showed that, unexpectedly, only half ...
DNA Methylation Analysis
... Infinium Assay for Methylation The Infinium Assay for Methylation (Figure 4) is based on highly multiplexed genotyping of bisulfite-converted genomic DNA. First, genomic DNA is treated with a bisulfite conversion kit. The result is DNA that contains unconverted cytosines (if they were methylated) or ...
... Infinium Assay for Methylation The Infinium Assay for Methylation (Figure 4) is based on highly multiplexed genotyping of bisulfite-converted genomic DNA. First, genomic DNA is treated with a bisulfite conversion kit. The result is DNA that contains unconverted cytosines (if they were methylated) or ...
- Wiley Online Library
... SMC (Structural Maintenance of Chromosomes) protein family, which has members present in all kingdoms of life. All SMC proteins appear to regulate chromosome morphology in some way or another. They have been implicated in chromosome condensation, recombination, repair and gene dosage compensation, a ...
... SMC (Structural Maintenance of Chromosomes) protein family, which has members present in all kingdoms of life. All SMC proteins appear to regulate chromosome morphology in some way or another. They have been implicated in chromosome condensation, recombination, repair and gene dosage compensation, a ...
Quantitative Analysis of the Kinetics of End
... The monomer-monomer interfaces are presumably the same at either end (and everywhere else) in the ®lament. As pointed out by Wegner (1982), the KD for monomer addition to either ®lament end cannot be different unless an independent source of chemical energy is provided to affect the binding to one e ...
... The monomer-monomer interfaces are presumably the same at either end (and everywhere else) in the ®lament. As pointed out by Wegner (1982), the KD for monomer addition to either ®lament end cannot be different unless an independent source of chemical energy is provided to affect the binding to one e ...
Specialized Transduction by Bacteriophage P22 in Salmonella typhimurium: Genetic and Physical Structure of the Transducing Genomes and the Prophage Attachment Site.
... genomes can be produced and propagated.-A physical and genetic map of the P22 genome near the prophage attachment site was constructed which shows that the insertion from the R-factor in P22Tc-10 is not a t the attachment site: it is therefore unlikely that P22Tc-10 was formed in an abnormal prophag ...
... genomes can be produced and propagated.-A physical and genetic map of the P22 genome near the prophage attachment site was constructed which shows that the insertion from the R-factor in P22Tc-10 is not a t the attachment site: it is therefore unlikely that P22Tc-10 was formed in an abnormal prophag ...
Enzyme Mechanisms - Illinois Institute of Technology
... Major groove is wider and shallower; minor groove is narrower and deeper ...
... Major groove is wider and shallower; minor groove is narrower and deeper ...
Maintenance of genomic integrity by p53: complementary
... preventing ®xation of DNA damage as mutations. This function of p53 led to the now famous coining of p53 as the `guardian of the genome' by Lane (1992). Although the main features of p53's role in maintaining the integrity of the genome seem to be outlined, there is still a lot to be learned as to h ...
... preventing ®xation of DNA damage as mutations. This function of p53 led to the now famous coining of p53 as the `guardian of the genome' by Lane (1992). Although the main features of p53's role in maintaining the integrity of the genome seem to be outlined, there is still a lot to be learned as to h ...
DNA
... These experiments suggest that the gene for eye color must be linked or co-inherited with the genes that determine the sex of the fly. This means that the genes occur on the same chromosome; more specifically it was the X chromosome. ...
... These experiments suggest that the gene for eye color must be linked or co-inherited with the genes that determine the sex of the fly. This means that the genes occur on the same chromosome; more specifically it was the X chromosome. ...
pyrimidine
... Incorporates DNA replication as an analytical tool for determining sequence Uses short primer that attaches to the 3’ end of the ssDNA, after which a specially engineered DNA polymerase Each vial includes one dideoxyXTP and 3 ordinary dXTPs; the dideoxyXTP will be incorporated but will halt synthesi ...
... Incorporates DNA replication as an analytical tool for determining sequence Uses short primer that attaches to the 3’ end of the ssDNA, after which a specially engineered DNA polymerase Each vial includes one dideoxyXTP and 3 ordinary dXTPs; the dideoxyXTP will be incorporated but will halt synthesi ...
Role of the p21 Protein in the Fanconi Anemia Pathway
... • Treat HCT116 p21+/+ and HCT116 p21-/- cells with the DNAcrosslinking agent mitomycin C (MMC). • Allow cells to grow for about 10 days. • Stain with crystal violet to quantify cells that survived. • Observe whether cells lacking p21 (HCT116 p21-/-) are more sensitive to MMC than wild type cells (HC ...
... • Treat HCT116 p21+/+ and HCT116 p21-/- cells with the DNAcrosslinking agent mitomycin C (MMC). • Allow cells to grow for about 10 days. • Stain with crystal violet to quantify cells that survived. • Observe whether cells lacking p21 (HCT116 p21-/-) are more sensitive to MMC than wild type cells (HC ...
The Rad50 Signature Motif: Essential to ATP Binding and
... The Mre11 protein contains a nuclease domain that is highly conserved and related to the lambda phosphatase family of phosphoesterases. The crystal structure of an Mre11 homolog from Pyrococcus furiosus shows five conserved motifs within the N-terminal half of Mre11 that coordinate two manganese ion ...
... The Mre11 protein contains a nuclease domain that is highly conserved and related to the lambda phosphatase family of phosphoesterases. The crystal structure of an Mre11 homolog from Pyrococcus furiosus shows five conserved motifs within the N-terminal half of Mre11 that coordinate two manganese ion ...
Nucleosome
A nucleosome is a basic unit of DNA packaging in eukaryotes, consisting of a segment of DNA wound in sequence around eight histone protein cores. This structure is often compared to thread wrapped around a spool.Nucleosomes form the fundamental repeating units of eukaryotic chromatin, which is used to pack the large eukaryotic genomes into the nucleus while still ensuring appropriate access to it (in mammalian cells approximately 2 m of linear DNA have to be packed into a nucleus of roughly 10 µm diameter). Nucleosomes are folded through a series of successively higher order structures to eventually form a chromosome; this both compacts DNA and creates an added layer of regulatory control, which ensures correct gene expression. Nucleosomes are thought to carry epigenetically inherited information in the form of covalent modifications of their core histones.Nucleosomes were observed as particles in the electron microscope by Don and Ada Olins and their existence and structure (as histone octamers surrounded by approximately 200 base pairs of DNA) were proposed by Roger Kornberg. The role of the nucleosome as a general gene repressor was demonstrated by Lorch et al. in vitro and by Han and Grunstein in vivo.The nucleosome core particle consists of approximately 147 base pairs of DNA wrapped in 1.67 left-handed superhelical turns around a histone octamer consisting of 2 copies each of the core histones H2A, H2B, H3, and H4. Core particles are connected by stretches of ""linker DNA"", which can be up to about 80 bp long. Technically, a nucleosome is defined as the core particle plus one of these linker regions; however the word is often synonymous with the core particle. Genome-wide nucleosome positioning maps are now available for many model organisms including mouse liver and brain.Linker histones such as H1 and its isoforms are involved in chromatin compaction and sit at the base of the nucleosome near the DNA entry and exit binding to the linker region of the DNA. Non-condensed nucleosomes without the linker histone resemble ""beads on a string of DNA"" under an electron microscope.In contrast to most eukaryotic cells, mature sperm cells largely use protamines to package their genomic DNA, most likely to achieve an even higher packaging ratio. Histone equivalents and a simplified chromatin structure have also been found in Archea, suggesting that eukaryotes are not the only organisms that use nucleosomes.