Recognition of Local DNA Structures by p53 Protein
... to cancer has been reviewed extensively [1–3]. The reason for such interest is obvious; more than 50% of all human tumors contain Tp53 mutations and inactivation of this gene plays a critical role in malignant transformation [1,3]. As a transcription factor, p53 regulates the expression of many down ...
... to cancer has been reviewed extensively [1–3]. The reason for such interest is obvious; more than 50% of all human tumors contain Tp53 mutations and inactivation of this gene plays a critical role in malignant transformation [1,3]. As a transcription factor, p53 regulates the expression of many down ...
Press release
... protocol and secondly also on the starting weight or volume. It also depends to a large extent on the sample material itself (leaf and seed material, animal tissue, hair, etc.). sbeadex - high-quality DNA preparations The oKtopure utilises the sbeadexTM extraction chemistry developed by LGC, which i ...
... protocol and secondly also on the starting weight or volume. It also depends to a large extent on the sample material itself (leaf and seed material, animal tissue, hair, etc.). sbeadex - high-quality DNA preparations The oKtopure utilises the sbeadexTM extraction chemistry developed by LGC, which i ...
Transcription
... • It is necessary to unwind the DNA so that the antisense strand to become accessible for base pairing and RNA synthesis. • Negative supercoiling enhances the transcription of many genes, since it facilitates unwinding. Some promoters are not. • Exceptional example: promters for the enzyme subunits ...
... • It is necessary to unwind the DNA so that the antisense strand to become accessible for base pairing and RNA synthesis. • Negative supercoiling enhances the transcription of many genes, since it facilitates unwinding. Some promoters are not. • Exceptional example: promters for the enzyme subunits ...
Structure and function of the GINS complex, a key component of the
... but two essential proteins, Sld2 and Sld3, have been shown to comprise the minimal set required for replication initiation [21,22]. Phosphorylation of Sld3 by S-CDK on Thr600 and Ser622 is essential for cell viability, substituting these two residues with non-phosphorylatable alanine residues blocks ...
... but two essential proteins, Sld2 and Sld3, have been shown to comprise the minimal set required for replication initiation [21,22]. Phosphorylation of Sld3 by S-CDK on Thr600 and Ser622 is essential for cell viability, substituting these two residues with non-phosphorylatable alanine residues blocks ...
Role of base-backbone and base-base interactions
... helical twist than the average (Table 1). In contrast, the YR steps in B-DNA and in the protein complexes are characterised with high standard deviations in roll, helical twist, and slide parameters, and thus these are more flexible than the others. The slide parameter at YR steps is positive on the ...
... helical twist than the average (Table 1). In contrast, the YR steps in B-DNA and in the protein complexes are characterised with high standard deviations in roll, helical twist, and slide parameters, and thus these are more flexible than the others. The slide parameter at YR steps is positive on the ...
Graph-based Methods for the Design of DNA - TUBdok
... silicon-based electronic units to perform calculations. The reliability of such computations strongly depends on the DNA sequences that represent units of information. Recently, the thermodynamic constraints, based on the free energy of hybridization between pairs of DNA single strands, are consider ...
... silicon-based electronic units to perform calculations. The reliability of such computations strongly depends on the DNA sequences that represent units of information. Recently, the thermodynamic constraints, based on the free energy of hybridization between pairs of DNA single strands, are consider ...
Epigenetic Regulation ofbdnfGene Transcription in the
... was used to determine the DNA methylation status of the bdnf gene. Methylation-specific PCR primers were designed using Methprimer software (http://www.urogene.org/methprimer/) (Li and Dahiya, 2002). CpG islands were found within promoters 1 and 2 and the exonic regions of the bdnf gene immediately ...
... was used to determine the DNA methylation status of the bdnf gene. Methylation-specific PCR primers were designed using Methprimer software (http://www.urogene.org/methprimer/) (Li and Dahiya, 2002). CpG islands were found within promoters 1 and 2 and the exonic regions of the bdnf gene immediately ...
BLOTTING.142
... To understand the basic concept of blotting techniques (Southern, northern, western) To know the main applications and advantages of each of the main types of blotting techniques To be familiar with the steps (in brief) for performing a blotting procedure To understand the major similarities ...
... To understand the basic concept of blotting techniques (Southern, northern, western) To know the main applications and advantages of each of the main types of blotting techniques To be familiar with the steps (in brief) for performing a blotting procedure To understand the major similarities ...
FLUORESCENT PROTEIN IN Escherichia coli
... paradigm to how infections were treated. Since then many other antiobiotics were developed, e.g. streptomycin against tuberculosis. This meant that the leading cause of death changed from being infections to being noninfectious diseases, i.e. cancer, stroke, etc. Meanwhile, extended use of antibioti ...
... paradigm to how infections were treated. Since then many other antiobiotics were developed, e.g. streptomycin against tuberculosis. This meant that the leading cause of death changed from being infections to being noninfectious diseases, i.e. cancer, stroke, etc. Meanwhile, extended use of antibioti ...
... Type 2 Diabetes Mellitus (T2DM) is a metabolic disorder influenced by interactions between genetic and environmental factors. Epigenetics conveys specific environmental influences into phenotypic traits through a variety of mechanisms that are often installed in early life, then persist in differenti ...
A genome-wide analysis of DNA methylation in buccal - VU-DARE
... restricting to the most variable CpG sites (for the top 10% CpGs of which methylation level varied most between subjects, the average heritability was 37%) 34. It was also found that gene body and intergenic regions showed higher average methylation levels, more variation between subjects, and highe ...
... restricting to the most variable CpG sites (for the top 10% CpGs of which methylation level varied most between subjects, the average heritability was 37%) 34. It was also found that gene body and intergenic regions showed higher average methylation levels, more variation between subjects, and highe ...
CYP2B6 NESTED PCR: A GOOD APPROACH FOR PATIENTS ON METHADONE Original Article
... block of ~112 kb in the middle of the CYP2A18P locus, between 19q12 and 19q13.2 [22,23-25]. CYP2B6 comprises nine exons encoding a protein of 491 amino acids [24]. In addition to the wildtype allele, several CYP2B6 allelic variants (C64T, C777A, A785G, C1459T, G516T, A785G, G516T, A785G, C1459T, A41 ...
... block of ~112 kb in the middle of the CYP2A18P locus, between 19q12 and 19q13.2 [22,23-25]. CYP2B6 comprises nine exons encoding a protein of 491 amino acids [24]. In addition to the wildtype allele, several CYP2B6 allelic variants (C64T, C777A, A785G, C1459T, G516T, A785G, G516T, A785G, C1459T, A41 ...
Bonus, a Drosophila TIF1 homologue, is a chromatin
... (GREWAL and MOAZED 2003). One of these families of proteins is the TIF1 family. The TIF1 family of proteins, TIF1α (LE DOUARIN et al. 1995), TIF1β [also called KAP-1 (FRIEDMAN et al. 1996) or KRIP-1 (KIM et al. 1996)], and TIF1γ (VENTURINI et al. 1999), are all structurally and functionally similar. ...
... (GREWAL and MOAZED 2003). One of these families of proteins is the TIF1 family. The TIF1 family of proteins, TIF1α (LE DOUARIN et al. 1995), TIF1β [also called KAP-1 (FRIEDMAN et al. 1996) or KRIP-1 (KIM et al. 1996)], and TIF1γ (VENTURINI et al. 1999), are all structurally and functionally similar. ...
Generation and analysis of mutated clonal scFv Jiya George
... monoclonal antibodies (mAbs) has the ability of to neutralize the virus. An epitope consisting of seven amino acids of the β2m protein designated as R7V produces antibodies that protect HIV infected people from progressing to AIDS. These protective antibodies, called anti-R7V antibodies, were able t ...
... monoclonal antibodies (mAbs) has the ability of to neutralize the virus. An epitope consisting of seven amino acids of the β2m protein designated as R7V produces antibodies that protect HIV infected people from progressing to AIDS. These protective antibodies, called anti-R7V antibodies, were able t ...
bacterial plasmids - Acta Medica Medianae
... Plasmids contain genes essential for plasmid function and their preservation in a host cell (the beginning and the control of replication). Some of them possess genes which control plasmid stability. There is a common opinion that plasmids are unnecessary for a growth of bacterial population and the ...
... Plasmids contain genes essential for plasmid function and their preservation in a host cell (the beginning and the control of replication). Some of them possess genes which control plasmid stability. There is a common opinion that plasmids are unnecessary for a growth of bacterial population and the ...
Plant centromeres: structure and control Eric J Richards and R Kelly
... repeats were localized to the centromere by chromosome in situ hybridization it is not possible to determine the position of the repeats relative to the functional centromere with certainty. Some corroborative evidence is available in Arabidopsis where the 180 bp repeat arrays map genetically to the ...
... repeats were localized to the centromere by chromosome in situ hybridization it is not possible to determine the position of the repeats relative to the functional centromere with certainty. Some corroborative evidence is available in Arabidopsis where the 180 bp repeat arrays map genetically to the ...
DNA Evolution 3.0 Administrator Guide
... The DNA Evolution Controller is software that runs on a networked Linux appliance. This software can run on a physical server (X Series, N Series) or on a virtual server (M Series). This core software component is included in all DNA Evolution products and is the foundation of the solution. The appl ...
... The DNA Evolution Controller is software that runs on a networked Linux appliance. This software can run on a physical server (X Series, N Series) or on a virtual server (M Series). This core software component is included in all DNA Evolution products and is the foundation of the solution. The appl ...
A pervasive role of histone acetyltransferases and deacetylases in
... or in combination to form the ‘histone code’, which is read by effector proteins to produce distinct biological outcomes [3]. Histone acetylation by histone acetyltransferases (HATs) is crucial for chromatin-compaction status and gene transcription (Box 2). Whereas increased histone acetylation prom ...
... or in combination to form the ‘histone code’, which is read by effector proteins to produce distinct biological outcomes [3]. Histone acetylation by histone acetyltransferases (HATs) is crucial for chromatin-compaction status and gene transcription (Box 2). Whereas increased histone acetylation prom ...
SNP
... Polymorphisms (SNPs) the variant sequence type has a frequency of at least 1% in the population. high frequency of SNPs in human genome: estimated ~1 SNP/Kb. ...
... Polymorphisms (SNPs) the variant sequence type has a frequency of at least 1% in the population. high frequency of SNPs in human genome: estimated ~1 SNP/Kb. ...
GROW`N`GLOW: THE ACE1 TWO-HYBRID
... valuable addition to the family of reporter genes for two-hybrid systems and makes a secondary screening of yeast colonies faster and more cost-effective than conventional LacZ assays. Detection of protein-protein interactions via the green fluorescent protein provides global screening of colonies w ...
... valuable addition to the family of reporter genes for two-hybrid systems and makes a secondary screening of yeast colonies faster and more cost-effective than conventional LacZ assays. Detection of protein-protein interactions via the green fluorescent protein provides global screening of colonies w ...
Defining characteristics of Tn5 Transposase non
... are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permission ...
... are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permission ...
The Large Loop Repair and Mismatch Repair Pathways
... in the RAD1-dependent LLR pathway springs from the known enzymatic roles of those proteins during mitotic DNA repair and the observed effects on meiotic recombination and DNA repair upon deletion of the LLR genes (Kirkpatrick and Petes 1997). Given the characterized activities of Rad1/10p and Msh2/3 ...
... in the RAD1-dependent LLR pathway springs from the known enzymatic roles of those proteins during mitotic DNA repair and the observed effects on meiotic recombination and DNA repair upon deletion of the LLR genes (Kirkpatrick and Petes 1997). Given the characterized activities of Rad1/10p and Msh2/3 ...
From the Department of Zoology, University of
... It is now generally agreed that chromosomes are multiple structures. Even the fight microscope has revealed two or even four units (chromonemata) in anaphase chromosomes. With the electron microscope these chromonemata are found to be still further subdivided. In the 200 A fibrils we seem to have re ...
... It is now generally agreed that chromosomes are multiple structures. Even the fight microscope has revealed two or even four units (chromonemata) in anaphase chromosomes. With the electron microscope these chromonemata are found to be still further subdivided. In the 200 A fibrils we seem to have re ...
Nucleosome
A nucleosome is a basic unit of DNA packaging in eukaryotes, consisting of a segment of DNA wound in sequence around eight histone protein cores. This structure is often compared to thread wrapped around a spool.Nucleosomes form the fundamental repeating units of eukaryotic chromatin, which is used to pack the large eukaryotic genomes into the nucleus while still ensuring appropriate access to it (in mammalian cells approximately 2 m of linear DNA have to be packed into a nucleus of roughly 10 µm diameter). Nucleosomes are folded through a series of successively higher order structures to eventually form a chromosome; this both compacts DNA and creates an added layer of regulatory control, which ensures correct gene expression. Nucleosomes are thought to carry epigenetically inherited information in the form of covalent modifications of their core histones.Nucleosomes were observed as particles in the electron microscope by Don and Ada Olins and their existence and structure (as histone octamers surrounded by approximately 200 base pairs of DNA) were proposed by Roger Kornberg. The role of the nucleosome as a general gene repressor was demonstrated by Lorch et al. in vitro and by Han and Grunstein in vivo.The nucleosome core particle consists of approximately 147 base pairs of DNA wrapped in 1.67 left-handed superhelical turns around a histone octamer consisting of 2 copies each of the core histones H2A, H2B, H3, and H4. Core particles are connected by stretches of ""linker DNA"", which can be up to about 80 bp long. Technically, a nucleosome is defined as the core particle plus one of these linker regions; however the word is often synonymous with the core particle. Genome-wide nucleosome positioning maps are now available for many model organisms including mouse liver and brain.Linker histones such as H1 and its isoforms are involved in chromatin compaction and sit at the base of the nucleosome near the DNA entry and exit binding to the linker region of the DNA. Non-condensed nucleosomes without the linker histone resemble ""beads on a string of DNA"" under an electron microscope.In contrast to most eukaryotic cells, mature sperm cells largely use protamines to package their genomic DNA, most likely to achieve an even higher packaging ratio. Histone equivalents and a simplified chromatin structure have also been found in Archea, suggesting that eukaryotes are not the only organisms that use nucleosomes.