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... Replication, DNA organization, and mismatch repair (MMR) can influence recombination. We examined the effects of altered replication due to a mutation in the polymerase d gene, long inverted repeats (LIRs) in motifs similar to those in higher eukaryotes, and MMR on intrachromosomal recombination bet ...
... Replication, DNA organization, and mismatch repair (MMR) can influence recombination. We examined the effects of altered replication due to a mutation in the polymerase d gene, long inverted repeats (LIRs) in motifs similar to those in higher eukaryotes, and MMR on intrachromosomal recombination bet ...
Are Restriction Enzymes Recognition Sites Underrepresented in the
... enzymes are sequence specific DNA cutters but the DNA sequence sometimes is not enzyme specific rendering a certain group of restriction enzymes that are ischizomers; restriction enzymes that belong to different or same class of the restriction enzymes and recognize and cleave at the same restrictio ...
... enzymes are sequence specific DNA cutters but the DNA sequence sometimes is not enzyme specific rendering a certain group of restriction enzymes that are ischizomers; restriction enzymes that belong to different or same class of the restriction enzymes and recognize and cleave at the same restrictio ...
Resolving Individuals Contributing Trace Amounts of DNA to Highly
... components of the mixture [8]. Nevertheless, these methods based on STRs expectedly suffer from limited power when using severely degraded DNA [8,9]. Mitochondrial DNA (mtDNA) based on hypervariable region sequencing is useful when analyzing degraded DNA due to its high copy number and improved stab ...
... components of the mixture [8]. Nevertheless, these methods based on STRs expectedly suffer from limited power when using severely degraded DNA [8,9]. Mitochondrial DNA (mtDNA) based on hypervariable region sequencing is useful when analyzing degraded DNA due to its high copy number and improved stab ...
Optimal estimation of diffusion coefficients from single
... on DNA from time-lapse measurements. The data have previously been analyzed using MSD-based methods [21]. We measure diffusion coefficients in the range 0.1–0.5 μm2 /s. We show that the DNA fluctuations induce a bias in the estimates of diffusion coefficients of up to 0.25 μm2 /s; i.e., we may overe ...
... on DNA from time-lapse measurements. The data have previously been analyzed using MSD-based methods [21]. We measure diffusion coefficients in the range 0.1–0.5 μm2 /s. We show that the DNA fluctuations induce a bias in the estimates of diffusion coefficients of up to 0.25 μm2 /s; i.e., we may overe ...
Primer on Molecular Biology
... • basic physical and functional units of heredity. • specific sequences of DNA bases that encode instructions on how to make proteins. • Proteins ...
... • basic physical and functional units of heredity. • specific sequences of DNA bases that encode instructions on how to make proteins. • Proteins ...
UCSD_PevznerMolecula.. - Purdue University :: Computer Science
... • basic physical and functional units of heredity. • specific sequences of DNA bases that encode instructions on how to make proteins. • Proteins ...
... • basic physical and functional units of heredity. • specific sequences of DNA bases that encode instructions on how to make proteins. • Proteins ...
Primer on Molecular Biology
... • basic physical and functional units of heredity. • specific sequences of DNA bases that encode instructions on how to make proteins. • Proteins ...
... • basic physical and functional units of heredity. • specific sequences of DNA bases that encode instructions on how to make proteins. • Proteins ...
The effecT of chlorinaTion of nucleoTide bases on The
... the normal vital functioning of the bacteria and its ability to divide normally, but it was found that these investigated organisms were not capable to transfer the modified fragments of DNA to the bacteria with natural DNA (containing thymine), in other words they cannot change the normal organisms ...
... the normal vital functioning of the bacteria and its ability to divide normally, but it was found that these investigated organisms were not capable to transfer the modified fragments of DNA to the bacteria with natural DNA (containing thymine), in other words they cannot change the normal organisms ...
COMPUTATIONAL MODELING OF CHARGE TRANSFER IN NUCLEOBASE-AROMATIC AMINO ACID COMPLEXES Cristina BUTCHOSA ROBLES
... Oxidative damage on DNA produces radical cation states. These states are also called electron ”holes”. Electron holes can migrate long distances through the nucleobases stack, due to conductivity properties of DNA. Finally, the cationic charge could be trapped and most probably a mutagenic lesion wi ...
... Oxidative damage on DNA produces radical cation states. These states are also called electron ”holes”. Electron holes can migrate long distances through the nucleobases stack, due to conductivity properties of DNA. Finally, the cationic charge could be trapped and most probably a mutagenic lesion wi ...
Human RIF1 and protein phosphatase 1 stimulate DNA replication
... Overall, the authors fill two gaps in the literature with this paper: 1. They confirm in humans much of which has been observed in yeast, which lends to RIF1's conserved and important role in genome regulation. 2. They observe a new role for RIF1 in origin stabilization, which adds another chapter t ...
... Overall, the authors fill two gaps in the literature with this paper: 1. They confirm in humans much of which has been observed in yeast, which lends to RIF1's conserved and important role in genome regulation. 2. They observe a new role for RIF1 in origin stabilization, which adds another chapter t ...
World Index of BioMolecular Visualization Resources
... A lecture-oriented chime 'slide show' about alkaline phosphatase. Buttons show particular views (these must usually be played in order) and a caption appears in the lowest frame. This shows ...
... A lecture-oriented chime 'slide show' about alkaline phosphatase. Buttons show particular views (these must usually be played in order) and a caption appears in the lowest frame. This shows ...
Fibrinogen Bern I: Substitution y 337 Asn + Lys Is
... Bern I, was detected in a clinically asymptomatic woman. Thrombin and reptilase clotting times were prolonged. Fibrinogen concentration was decreased when measured by the functional assay but was normal by the heat precipitation method (Table 1). Family study confirmed the hereditary nature of this ...
... Bern I, was detected in a clinically asymptomatic woman. Thrombin and reptilase clotting times were prolonged. Fibrinogen concentration was decreased when measured by the functional assay but was normal by the heat precipitation method (Table 1). Family study confirmed the hereditary nature of this ...
Downloaded - Laboratoire de Chimie Théorique
... are either potent mutagens or inhibitors. We used structural and topological analyses based on ab initio QM/MM calculations to study human DNA polymerase l (Poll) with different metals in the active site. Our results indicate a slightly longer O3 0 –Pa distance (B3.6 Å) for most inhibitor cations co ...
... are either potent mutagens or inhibitors. We used structural and topological analyses based on ab initio QM/MM calculations to study human DNA polymerase l (Poll) with different metals in the active site. Our results indicate a slightly longer O3 0 –Pa distance (B3.6 Å) for most inhibitor cations co ...
Susan Viggiano and Michael S. Cosgrove
... Although MLL1 has served as a paradigm for the mechanism of action of human SET1 family enzymes, several recent reports suggest different family members are controlled by distinct regulatory mechanisms. For example, although the MLL1 core complex has predominantly mono- and dimethyltransferase activ ...
... Although MLL1 has served as a paradigm for the mechanism of action of human SET1 family enzymes, several recent reports suggest different family members are controlled by distinct regulatory mechanisms. For example, although the MLL1 core complex has predominantly mono- and dimethyltransferase activ ...
Supplementary Materials and methods (doc 154K)
... assessed. We used the protocol previously described by Itoh et al. for the mobilization of plasmid pNI111 by RP4 (Itoh et al 1991). P. aeruginosa PAO1-GFP (resistant to gentamycin and chloramphenicol) was used as a recipient strain. Mating experiments were performed three independent times on LB aga ...
... assessed. We used the protocol previously described by Itoh et al. for the mobilization of plasmid pNI111 by RP4 (Itoh et al 1991). P. aeruginosa PAO1-GFP (resistant to gentamycin and chloramphenicol) was used as a recipient strain. Mating experiments were performed three independent times on LB aga ...
The DNA Binding Properties of Saccharomyces cerevisiae Rad51
... only at pH values lower than 6.8; in this case, the apparent binding stoichiometry covers the range of 1 protein monomer per 6 –9 nucleotides or base pairs. Therefore, Rad51 protein displays two distinct modes of DNA binding. These binding modes are not inter-convertible; however, their initial sele ...
... only at pH values lower than 6.8; in this case, the apparent binding stoichiometry covers the range of 1 protein monomer per 6 –9 nucleotides or base pairs. Therefore, Rad51 protein displays two distinct modes of DNA binding. These binding modes are not inter-convertible; however, their initial sele ...
Taxonomic characterization of Ochrobactrum sp. isolates from soil
... cluster, a separate DNA–DNA reassociation group and showed, as a biological particularity, a strict preference for the rhizoplane as habitat. Diagnostic phenotypic characters were found. This indicated that REP group J strains represent a further new species, although phylogenetic analyses using 16S ...
... cluster, a separate DNA–DNA reassociation group and showed, as a biological particularity, a strict preference for the rhizoplane as habitat. Diagnostic phenotypic characters were found. This indicated that REP group J strains represent a further new species, although phylogenetic analyses using 16S ...
arXiv:0708.2724v1 [cond-mat.other] 20 Aug 2007
... chemical techniques and physical differences of strands of DNA. Most importantly, these proposals challenge our understanding of, and ability to manipulate and probe, physical processes at the interface between solids, liquids, and biomolecules down to the nanometer scale regime (Di Ventra et al., 2 ...
... chemical techniques and physical differences of strands of DNA. Most importantly, these proposals challenge our understanding of, and ability to manipulate and probe, physical processes at the interface between solids, liquids, and biomolecules down to the nanometer scale regime (Di Ventra et al., 2 ...
Bridging the transgenerational gap with epigenetic memory
... in mammals during the 1980s provided the first indication that epigenetic modifications, such as DNA methylation, were not entirely erased between generations and could underlie transgenerational epigenetic inheritance [6–11]. It was also around this time that several cases of transgenerational epig ...
... in mammals during the 1980s provided the first indication that epigenetic modifications, such as DNA methylation, were not entirely erased between generations and could underlie transgenerational epigenetic inheritance [6–11]. It was also around this time that several cases of transgenerational epig ...
Replication of damaged DNA in vitro is blocked
... interactions of PAb421 with the p53 C-terminus can in some cases stimulate its ability to bind to DNA (41). It has also been shown that p53 puri®ed in the absence of reducing agents (HA±p53 [¯u, ±DTT]) is inert in DNA binding (39,42,43). In contrast, as is well-documented, p53 lacking the C-terminus ...
... interactions of PAb421 with the p53 C-terminus can in some cases stimulate its ability to bind to DNA (41). It has also been shown that p53 puri®ed in the absence of reducing agents (HA±p53 [¯u, ±DTT]) is inert in DNA binding (39,42,43). In contrast, as is well-documented, p53 lacking the C-terminus ...
1 MCM10 MEDIATES THE INTERACTION BETWEEN DNA
... defect both at the telomere (Figure 1A) as well as at the HMR (Figure 1B). This observation raised the possibility that some pre-RC proteins may interact with silencing factors. Indeed, when tested in a two-hybrid system using LexA binding domain (BTM) and Gal4 activation domain (GAD) fusion protein ...
... defect both at the telomere (Figure 1A) as well as at the HMR (Figure 1B). This observation raised the possibility that some pre-RC proteins may interact with silencing factors. Indeed, when tested in a two-hybrid system using LexA binding domain (BTM) and Gal4 activation domain (GAD) fusion protein ...
Nucleosome
A nucleosome is a basic unit of DNA packaging in eukaryotes, consisting of a segment of DNA wound in sequence around eight histone protein cores. This structure is often compared to thread wrapped around a spool.Nucleosomes form the fundamental repeating units of eukaryotic chromatin, which is used to pack the large eukaryotic genomes into the nucleus while still ensuring appropriate access to it (in mammalian cells approximately 2 m of linear DNA have to be packed into a nucleus of roughly 10 µm diameter). Nucleosomes are folded through a series of successively higher order structures to eventually form a chromosome; this both compacts DNA and creates an added layer of regulatory control, which ensures correct gene expression. Nucleosomes are thought to carry epigenetically inherited information in the form of covalent modifications of their core histones.Nucleosomes were observed as particles in the electron microscope by Don and Ada Olins and their existence and structure (as histone octamers surrounded by approximately 200 base pairs of DNA) were proposed by Roger Kornberg. The role of the nucleosome as a general gene repressor was demonstrated by Lorch et al. in vitro and by Han and Grunstein in vivo.The nucleosome core particle consists of approximately 147 base pairs of DNA wrapped in 1.67 left-handed superhelical turns around a histone octamer consisting of 2 copies each of the core histones H2A, H2B, H3, and H4. Core particles are connected by stretches of ""linker DNA"", which can be up to about 80 bp long. Technically, a nucleosome is defined as the core particle plus one of these linker regions; however the word is often synonymous with the core particle. Genome-wide nucleosome positioning maps are now available for many model organisms including mouse liver and brain.Linker histones such as H1 and its isoforms are involved in chromatin compaction and sit at the base of the nucleosome near the DNA entry and exit binding to the linker region of the DNA. Non-condensed nucleosomes without the linker histone resemble ""beads on a string of DNA"" under an electron microscope.In contrast to most eukaryotic cells, mature sperm cells largely use protamines to package their genomic DNA, most likely to achieve an even higher packaging ratio. Histone equivalents and a simplified chromatin structure have also been found in Archea, suggesting that eukaryotes are not the only organisms that use nucleosomes.