A prophage-encoded actin-like protein required for efficient viral
... the excision of the viral DNA from the genome, replication, virion assembly and lysis of the host cell (3,4). Recent reports revealed that viral replication in prokaryotes appears to be organized at specific intracellular locations and this process relies on the action of cytoskeletal proteins (5). ...
... the excision of the viral DNA from the genome, replication, virion assembly and lysis of the host cell (3,4). Recent reports revealed that viral replication in prokaryotes appears to be organized at specific intracellular locations and this process relies on the action of cytoskeletal proteins (5). ...
Site-Specific Integration of Transgenes in
... All events were then evaluated by four constructspecific qPCR analyses (Fig. 1) to check for DNA recombination at the FRT1 site and the presence of the target, donor, and flp DNA (Table II), followed by five border-specific PCR analyses specific to each target line using the 5# border, 3# border, an ...
... All events were then evaluated by four constructspecific qPCR analyses (Fig. 1) to check for DNA recombination at the FRT1 site and the presence of the target, donor, and flp DNA (Table II), followed by five border-specific PCR analyses specific to each target line using the 5# border, 3# border, an ...
BlastLecture8
... • Search dbEST if you want to know if your sequence is expressed • UniProt90: no seq is more than 90% ident to any other (for an uncluttered tree) also UniProt50 ...
... • Search dbEST if you want to know if your sequence is expressed • UniProt90: no seq is more than 90% ident to any other (for an uncluttered tree) also UniProt50 ...
PDF
... The process of generating differentiated cell types performing specific effector functions from their respective undifferentiated precursors is dictated by extracellular signals, which alter the host cell’s capacity to perform cellular functions. One major mechanism for bringing about such changes i ...
... The process of generating differentiated cell types performing specific effector functions from their respective undifferentiated precursors is dictated by extracellular signals, which alter the host cell’s capacity to perform cellular functions. One major mechanism for bringing about such changes i ...
Isolation of the b-tubulin Gene From Yeast and Demonstration of its Essential Function in vivo.
... showed that these cDNAs do not hybridize to yeast DNA under conditions that give a strong signal against higher eucaryotic DNA (Cleveland et al., 1980). To find optimum hybridization conditions, the stringency of the hybridization reaction was changed by adding increasing amounts of formamide (0 to ...
... showed that these cDNAs do not hybridize to yeast DNA under conditions that give a strong signal against higher eucaryotic DNA (Cleveland et al., 1980). To find optimum hybridization conditions, the stringency of the hybridization reaction was changed by adding increasing amounts of formamide (0 to ...
MB206_fhs_lnt_001.1_AT_May09
... A typical chromosome is a few million base pairs in length. Most bacterial species contain a single type of chromosome, but it may be present in multiple copies. ...
... A typical chromosome is a few million base pairs in length. Most bacterial species contain a single type of chromosome, but it may be present in multiple copies. ...
Effects of Salt Concentrations and Bending Energy on the Extent of
... For any lower value of cPEG there is a pressure difference and hence a net force (outward) on the confining walls, because an insufficient amount of water has been drawn out of the DNA solution to lower its hydrostatic pressure to 1 atm. Let cPEG be such an intermediate value: 0,cPEG ,cPEG . Suppos ...
... For any lower value of cPEG there is a pressure difference and hence a net force (outward) on the confining walls, because an insufficient amount of water has been drawn out of the DNA solution to lower its hydrostatic pressure to 1 atm. Let cPEG be such an intermediate value: 0,cPEG ,cPEG . Suppos ...
Genetic Studies of Recombining DNA in
... the donor DNA template, followed by insertion in a chromosome and rejoining of broken ends, we could well understand why the intracellular concentration of opt-r genes would decrease during recombination. We have every reason to believe that the LE mutations in the amiA-r region behave as does the o ...
... the donor DNA template, followed by insertion in a chromosome and rejoining of broken ends, we could well understand why the intracellular concentration of opt-r genes would decrease during recombination. We have every reason to believe that the LE mutations in the amiA-r region behave as does the o ...
Protocols for 16S rDNA Array Analyses of Microbial
... KEY WORDS: rRNA, rDNA, DNA array, microbial community DOMAINS: methods and protocols There has been a tremendous development of DNA array-based applications[1] since the pioneer work by P. Brown[2]. Generally, DNA arrays are used either for global gene expression analyses or for screening of genes p ...
... KEY WORDS: rRNA, rDNA, DNA array, microbial community DOMAINS: methods and protocols There has been a tremendous development of DNA array-based applications[1] since the pioneer work by P. Brown[2]. Generally, DNA arrays are used either for global gene expression analyses or for screening of genes p ...
Document
... Biomolecular structure is determined by a combination of covalent and noncovalent bonds Covalent bonds are static entities which are little effected by environment Noncovalent bonds (hydrophobic interactions, hydrogen bonding, and electrostatic attraction) exist in a dynamic equilibrium, and thus ca ...
... Biomolecular structure is determined by a combination of covalent and noncovalent bonds Covalent bonds are static entities which are little effected by environment Noncovalent bonds (hydrophobic interactions, hydrogen bonding, and electrostatic attraction) exist in a dynamic equilibrium, and thus ca ...
Checkpoint responses to unusual structures formed by DNA repeats
... instability [2]. It is now widely believed that the instability phenomena associated with structureprone sequences such as TNRs, DNA palindromes, and certain common fragile sites involve the formation of an ADS in vivo. Although several experimentally supported hypotheses for ADS-mediated genomic in ...
... instability [2]. It is now widely believed that the instability phenomena associated with structureprone sequences such as TNRs, DNA palindromes, and certain common fragile sites involve the formation of an ADS in vivo. Although several experimentally supported hypotheses for ADS-mediated genomic in ...
PDF
... cus, and several eukaryotic genomes. ung gene was determined and is presented in this paper. The open reading frame of the gene, confirmed by N-terminal protein sequence analysis, codes for a protein of25,664 Da DNA glycosylases excise damaged or unconventional bases which contains a single cysteine ...
... cus, and several eukaryotic genomes. ung gene was determined and is presented in this paper. The open reading frame of the gene, confirmed by N-terminal protein sequence analysis, codes for a protein of25,664 Da DNA glycosylases excise damaged or unconventional bases which contains a single cysteine ...
Unit 4 Schedule
... Genetic material can change and may result in a form of a gene that was never present in a family appearing in the phenotype. Mutations change the instructions that are encoded in genes by changing the sequence of bases in DNA. Substances that can cause changes in DNA are known as mutagens. If an am ...
... Genetic material can change and may result in a form of a gene that was never present in a family appearing in the phenotype. Mutations change the instructions that are encoded in genes by changing the sequence of bases in DNA. Substances that can cause changes in DNA are known as mutagens. If an am ...
Crystal Structure of a 70 Subunit Fragment from
... The backbone trace is displayed as a white tube, showing the cluster of four helices comprising the conserved regions. A partially transparent, solvent-accessible surface encloses the structure. The large black labels denote the helices. The view is directly at the face containing helices 12a and 12 ...
... The backbone trace is displayed as a white tube, showing the cluster of four helices comprising the conserved regions. A partially transparent, solvent-accessible surface encloses the structure. The large black labels denote the helices. The view is directly at the face containing helices 12a and 12 ...
DNA mutation bracelets
... 1) What changes in the RNA sequence were caused by the changes in the DNA? When the DNA sequence was changed, the RNA reading frame was shifted. 2) What changes in amino acids were caused by the changes in the DNA? Different amino acid were coded after the deletion shifted the sequence. 3) What happ ...
... 1) What changes in the RNA sequence were caused by the changes in the DNA? When the DNA sequence was changed, the RNA reading frame was shifted. 2) What changes in amino acids were caused by the changes in the DNA? Different amino acid were coded after the deletion shifted the sequence. 3) What happ ...
LP - Columbia University
... Both sexes have 22 pairs of chromosomes that look the same regardless of sex, but the 23rd pair is not the same in both sexes. In females, the 23rd pair consists of 2 large chromosomes that look alike. In males the 23rd pair consists of a large and a small chromosome that do not look alike but act ...
... Both sexes have 22 pairs of chromosomes that look the same regardless of sex, but the 23rd pair is not the same in both sexes. In females, the 23rd pair consists of 2 large chromosomes that look alike. In males the 23rd pair consists of a large and a small chromosome that do not look alike but act ...
Genetics Images/plasmids.jpg - KSU Faculty Member websites
... replicated is inserted in a plasmid. These plasmids contain, in addition to the inserted gene, one or more genes capable of providing antibiotic resistance to the bacterium that harbors them. The plasmids are next inserted into bacteria by a process called transformation, which are then grown on spe ...
... replicated is inserted in a plasmid. These plasmids contain, in addition to the inserted gene, one or more genes capable of providing antibiotic resistance to the bacterium that harbors them. The plasmids are next inserted into bacteria by a process called transformation, which are then grown on spe ...
GENECLEAN® Kit
... When working with radio-labeled DNA, add 1 µl of Label Block to each 10 µl of GLASSMILK® and incubate for 5 minutes at room temperature before adding to the DNA/NaI solution in the next step. 5. Add GLASSMILK® to the DNA/NaI solution as calculated in Step 3. Vortex gently or stir with a pipet tip to ...
... When working with radio-labeled DNA, add 1 µl of Label Block to each 10 µl of GLASSMILK® and incubate for 5 minutes at room temperature before adding to the DNA/NaI solution in the next step. 5. Add GLASSMILK® to the DNA/NaI solution as calculated in Step 3. Vortex gently or stir with a pipet tip to ...
Duplication of Small Segments Within the Major
... probes, indicating translocation within the M-bcr. Bg/ II/ BumHl double-digested DNA from this group was screened for M-bcr rearrangement by Southern analysis with the 5’ and 3’ probes. These studies separated those cases with Mbcr translocations into three groups. The first group (9 patients) demon ...
... probes, indicating translocation within the M-bcr. Bg/ II/ BumHl double-digested DNA from this group was screened for M-bcr rearrangement by Southern analysis with the 5’ and 3’ probes. These studies separated those cases with Mbcr translocations into three groups. The first group (9 patients) demon ...
ISOLATE II PCR and Gel Kit
... The Binding Buffer CB is sufficiently buffered to maintain an optimal pH of 5.0–6.0. It will even bind small DNA fragments to the silica membrane of the ISOLATE II PCR and Gel Columns, for all standard PCR reaction buffers or agarose gel buffer systems. In addition, the colored binding buffer helps ...
... The Binding Buffer CB is sufficiently buffered to maintain an optimal pH of 5.0–6.0. It will even bind small DNA fragments to the silica membrane of the ISOLATE II PCR and Gel Columns, for all standard PCR reaction buffers or agarose gel buffer systems. In addition, the colored binding buffer helps ...
Chpt7_RepairDNA.doc
... degraded when they are no longer needed or damaged, and smaller molecules such as sugars are metabolized to compounds to make or store energy. In contrast, DNA is the most stable biological molecule known, befitting its role in storage of genetic information. The DNA is passed from one generation to ...
... degraded when they are no longer needed or damaged, and smaller molecules such as sugars are metabolized to compounds to make or store energy. In contrast, DNA is the most stable biological molecule known, befitting its role in storage of genetic information. The DNA is passed from one generation to ...
A Histone H3.3-like Gene Specifically Expressed in the Vegetative
... pollen and mature pollen, whose generative cells were spindle shaped and yellow (Fig. 4d, e). Thus, the YAH3 signal was only detected in generative cells from 75 mm buds and was never detected in vegetative cells during pollen development. Figure 5 shows the results of in situ hybridization with MPH ...
... pollen and mature pollen, whose generative cells were spindle shaped and yellow (Fig. 4d, e). Thus, the YAH3 signal was only detected in generative cells from 75 mm buds and was never detected in vegetative cells during pollen development. Figure 5 shows the results of in situ hybridization with MPH ...
Nucleosome
A nucleosome is a basic unit of DNA packaging in eukaryotes, consisting of a segment of DNA wound in sequence around eight histone protein cores. This structure is often compared to thread wrapped around a spool.Nucleosomes form the fundamental repeating units of eukaryotic chromatin, which is used to pack the large eukaryotic genomes into the nucleus while still ensuring appropriate access to it (in mammalian cells approximately 2 m of linear DNA have to be packed into a nucleus of roughly 10 µm diameter). Nucleosomes are folded through a series of successively higher order structures to eventually form a chromosome; this both compacts DNA and creates an added layer of regulatory control, which ensures correct gene expression. Nucleosomes are thought to carry epigenetically inherited information in the form of covalent modifications of their core histones.Nucleosomes were observed as particles in the electron microscope by Don and Ada Olins and their existence and structure (as histone octamers surrounded by approximately 200 base pairs of DNA) were proposed by Roger Kornberg. The role of the nucleosome as a general gene repressor was demonstrated by Lorch et al. in vitro and by Han and Grunstein in vivo.The nucleosome core particle consists of approximately 147 base pairs of DNA wrapped in 1.67 left-handed superhelical turns around a histone octamer consisting of 2 copies each of the core histones H2A, H2B, H3, and H4. Core particles are connected by stretches of ""linker DNA"", which can be up to about 80 bp long. Technically, a nucleosome is defined as the core particle plus one of these linker regions; however the word is often synonymous with the core particle. Genome-wide nucleosome positioning maps are now available for many model organisms including mouse liver and brain.Linker histones such as H1 and its isoforms are involved in chromatin compaction and sit at the base of the nucleosome near the DNA entry and exit binding to the linker region of the DNA. Non-condensed nucleosomes without the linker histone resemble ""beads on a string of DNA"" under an electron microscope.In contrast to most eukaryotic cells, mature sperm cells largely use protamines to package their genomic DNA, most likely to achieve an even higher packaging ratio. Histone equivalents and a simplified chromatin structure have also been found in Archea, suggesting that eukaryotes are not the only organisms that use nucleosomes.