RAD50, an SMC family member with multiple roles in DNA break
... (NTP)-binding motifs at their amino- and carboxyterminal ends, respectively. These motifs are separated by long stretches of amino acids that form an extended coiled-coil structure. The coiled coils fold back on themselves to form intramolecular association of the ATPase domains at one end and a so- ...
... (NTP)-binding motifs at their amino- and carboxyterminal ends, respectively. These motifs are separated by long stretches of amino acids that form an extended coiled-coil structure. The coiled coils fold back on themselves to form intramolecular association of the ATPase domains at one end and a so- ...
Physical interaction between proliferating cell nuclear antigen and
... Furuedai, Suita, Osaka 565-0874, Japan ...
... Furuedai, Suita, Osaka 565-0874, Japan ...
Database homology searching
... • Search UniProt to know what the hits are • Search dbEST to know if your sequence is expressed • UniProt90: no seq is more than 90% ident to any other (for an uncluttered tree) also UniProt50 ...
... • Search UniProt to know what the hits are • Search dbEST to know if your sequence is expressed • UniProt90: no seq is more than 90% ident to any other (for an uncluttered tree) also UniProt50 ...
Preparation of MyoD mRNA for the differentiation of stem cells into
... from ϕ2.5 promoters. Different RNA polymerases require different conditions for optimal enzymatic activity. T7 RNA polymerase requires a reducing environment, since it contains a sulfhydryl group that is essential for its enzymatic activity11. ...
... from ϕ2.5 promoters. Different RNA polymerases require different conditions for optimal enzymatic activity. T7 RNA polymerase requires a reducing environment, since it contains a sulfhydryl group that is essential for its enzymatic activity11. ...
PTC Genetics Lab Student Worksheet
... Electrophoresis is a technique used in many areas of science to analyze and separate samples by applying a constant electric field. Biologists or forensic scientists can use this technology to separate mixtures of DNA or dyes into each component based on size and electrical charge. The gel in gel el ...
... Electrophoresis is a technique used in many areas of science to analyze and separate samples by applying a constant electric field. Biologists or forensic scientists can use this technology to separate mixtures of DNA or dyes into each component based on size and electrical charge. The gel in gel el ...
University of Debrecen - DEA
... and photosynthesis, respectively. The key processes of genetic information flow are partitioned in the eukaryotic cells: DNA replication and transcription (RNA synthesis) occur in the nucleus while translation (protein synthesis) occurs in the cytoplasm. In contrast to eukaryotic cells, prokaryotic ...
... and photosynthesis, respectively. The key processes of genetic information flow are partitioned in the eukaryotic cells: DNA replication and transcription (RNA synthesis) occur in the nucleus while translation (protein synthesis) occurs in the cytoplasm. In contrast to eukaryotic cells, prokaryotic ...
Effect of defects on thermal denaturation of DNA Oligomers
... used in the case of homogeneous chain is no longer valid. Attempts have, however, been made to use the model Hamiltonian of Eq.(1) for heterogeneous chains either by modelling the heterogeneity with quenched disorder [6] or by properly choosing basis sets of orthonormal functions for the kernels ap ...
... used in the case of homogeneous chain is no longer valid. Attempts have, however, been made to use the model Hamiltonian of Eq.(1) for heterogeneous chains either by modelling the heterogeneity with quenched disorder [6] or by properly choosing basis sets of orthonormal functions for the kernels ap ...
Chapter 20
... electrophoresis with nucleic acid hybridization, allowing researchers to find a specific human gene. – Specific DNA fragments can be identified by Southern blotting, using labeled probes that hybridize to the DNA immobilized on a “blot” of gel – This technique is specific enough to find differences ...
... electrophoresis with nucleic acid hybridization, allowing researchers to find a specific human gene. – Specific DNA fragments can be identified by Southern blotting, using labeled probes that hybridize to the DNA immobilized on a “blot” of gel – This technique is specific enough to find differences ...
Question Paper Code 57/3
... The replication begins in definite regions which are called the origin of replication , Replication occurs within a small opening of the DNA referred to as Y shaped replication fork (uncoiling of DNA is by some enzymes eg Helicase and topoisomerase), Polymerisation of the nucleotides are catalysed b ...
... The replication begins in definite regions which are called the origin of replication , Replication occurs within a small opening of the DNA referred to as Y shaped replication fork (uncoiling of DNA is by some enzymes eg Helicase and topoisomerase), Polymerisation of the nucleotides are catalysed b ...
This article appeared in a journal published by Elsevier. The
... Several researches in vaccine development against A. pleuropneumoniae have been stimulated in the past years due to the economic impact of this disease in swine industry. The state-ofthe-art of A. pleuropneumoniae vaccines was reviewed and showed that it is promising in vaccine research and developm ...
... Several researches in vaccine development against A. pleuropneumoniae have been stimulated in the past years due to the economic impact of this disease in swine industry. The state-ofthe-art of A. pleuropneumoniae vaccines was reviewed and showed that it is promising in vaccine research and developm ...
Analysis of Similarities/Dissimilarities of DNA Sequences Based on a
... -501that most of the 11 sequences are rich in dinucleotide of TG, while lack of TA and CG, information of other dinucleotides can also be inspected intuitively according to the colorbar. In addition, we can see that Gorilla and Chimpanzee have the most similar compositions and densities of dinucleo ...
... -501that most of the 11 sequences are rich in dinucleotide of TG, while lack of TA and CG, information of other dinucleotides can also be inspected intuitively according to the colorbar. In addition, we can see that Gorilla and Chimpanzee have the most similar compositions and densities of dinucleo ...
The Modular Structure and Function of the Wheat HI Promoter with S
... phobic 'central globular domain', and Lys-rich carboxy terminal domain. Amino acid sequence comparisons of the wheat TH315 protein with other plant His showed that similarity was rather restricted to the central globular domain (Fig. 3A), ranging from 80% for wcHl-1 (Yang et al. 1991) to 50% for Ara ...
... phobic 'central globular domain', and Lys-rich carboxy terminal domain. Amino acid sequence comparisons of the wheat TH315 protein with other plant His showed that similarity was rather restricted to the central globular domain (Fig. 3A), ranging from 80% for wcHl-1 (Yang et al. 1991) to 50% for Ara ...
PcrA Helicase Tightly Couples ATP Hydrolysis to Unwinding Double
... In practice, the biphasic nature of these traces and small signal amplitudes made full analysis difficult, in particular obtaining accurate rate information for the first phase. To overcome this, experiments were repeated using similar experimental conditions as those described above, but rapidly mi ...
... In practice, the biphasic nature of these traces and small signal amplitudes made full analysis difficult, in particular obtaining accurate rate information for the first phase. To overcome this, experiments were repeated using similar experimental conditions as those described above, but rapidly mi ...
Chapter 19: DNA Ligases - DNA Replication and Human
... that contains only the latter, leaky mutation expressed in hemizygous or homozygous form. 46BR cells show inefficient joining of Okazaki fragments and anomalous gap filling during excision repair (Prigent et al. 1994). However, V(D)J joining seems to proceed normally in 46BR cells (Hsieh et al. 1993 ...
... that contains only the latter, leaky mutation expressed in hemizygous or homozygous form. 46BR cells show inefficient joining of Okazaki fragments and anomalous gap filling during excision repair (Prigent et al. 1994). However, V(D)J joining seems to proceed normally in 46BR cells (Hsieh et al. 1993 ...
... b) Compare and contrast the structure of the mainchain (backbone) and sidechain groups in these two structures (a labeled drawing is fine) (5 pts). In an α-helix the mainchain follow a helical pattern with the sidechains pointing out. ( 2 ½ pts) In a β -sheet the strands are arranged in a parallel ( ...
Protocol
... chemical synthesis (for siRNA) and vector-based expression (for shRNA) [8-12]. While effective in triggering RNAi, the synthetic siRNAs are expensive and only mediate transient knockdown effect. In contrast, the promoter driven expression of short hairpin RNAs (shRNAs) in cells is more cost-effectiv ...
... chemical synthesis (for siRNA) and vector-based expression (for shRNA) [8-12]. While effective in triggering RNAi, the synthetic siRNAs are expensive and only mediate transient knockdown effect. In contrast, the promoter driven expression of short hairpin RNAs (shRNAs) in cells is more cost-effectiv ...
Sterile, 24-well tissue culture plates are filled with melted minimal ... 1.0 ml per well using a repeating syringe. After the...
... The latter is presumed to be a duplication bearing class, while the unpigmented ascospores probably represent the corresponding segregants carrying a deficiency. The wild type did not segregate. This suggests that mts(MN9) is involved in a translocation and is linked with cpc-1. Since further mappin ...
... The latter is presumed to be a duplication bearing class, while the unpigmented ascospores probably represent the corresponding segregants carrying a deficiency. The wild type did not segregate. This suggests that mts(MN9) is involved in a translocation and is linked with cpc-1. Since further mappin ...
An Introduction to Genetic Analysis Chapter 16 Mechanisms of Gene
... Large deletions (more than a few base pairs) constitute a sizable fraction of sponta-neous mutations, as shown in Figure 16-5. The majority, although not all, of the deletions occur at repeated sequences. Figure 16-6 shows the results for the first 12 deletions analyzed at the DNA sequence level, pr ...
... Large deletions (more than a few base pairs) constitute a sizable fraction of sponta-neous mutations, as shown in Figure 16-5. The majority, although not all, of the deletions occur at repeated sequences. Figure 16-6 shows the results for the first 12 deletions analyzed at the DNA sequence level, pr ...
sex chromosomes
... • For most genes it is a reasonable assumption that a specific allele will have the same effect regardless of whether it was inherited from the mother or father. • However, for some traits in mammals, it does depend on which parent passed along the alleles for those traits. – The genes involved may ...
... • For most genes it is a reasonable assumption that a specific allele will have the same effect regardless of whether it was inherited from the mother or father. • However, for some traits in mammals, it does depend on which parent passed along the alleles for those traits. – The genes involved may ...
... 3. Please do four of the following five choices. Read each choice carefully, some ask you to discuss two items while others give you a choice between one item or another. (20 pts) (Two choices are on the next page) Choice A: Discuss the role of the hydrophobic effect in the formation of the folded s ...
Activity Name - Science4Inquiry.com
... DNA Replication Assessment 1. Which of the following accurately reflects the correct order of DNA replication? a. Primase creates an RNA primer, helicase unzips the DNA, DNA polymerase adds nucleotides and creates new DNA, DNA polymerase fills in the gaps, DNA ligase seals the fragments of DNA, exon ...
... DNA Replication Assessment 1. Which of the following accurately reflects the correct order of DNA replication? a. Primase creates an RNA primer, helicase unzips the DNA, DNA polymerase adds nucleotides and creates new DNA, DNA polymerase fills in the gaps, DNA ligase seals the fragments of DNA, exon ...
What Makes the “Blue” in Blueberries?
... • Localizes to recently replicated DNA in mitotically cycling and endocycling cells ...
... • Localizes to recently replicated DNA in mitotically cycling and endocycling cells ...
Nucleosome
A nucleosome is a basic unit of DNA packaging in eukaryotes, consisting of a segment of DNA wound in sequence around eight histone protein cores. This structure is often compared to thread wrapped around a spool.Nucleosomes form the fundamental repeating units of eukaryotic chromatin, which is used to pack the large eukaryotic genomes into the nucleus while still ensuring appropriate access to it (in mammalian cells approximately 2 m of linear DNA have to be packed into a nucleus of roughly 10 µm diameter). Nucleosomes are folded through a series of successively higher order structures to eventually form a chromosome; this both compacts DNA and creates an added layer of regulatory control, which ensures correct gene expression. Nucleosomes are thought to carry epigenetically inherited information in the form of covalent modifications of their core histones.Nucleosomes were observed as particles in the electron microscope by Don and Ada Olins and their existence and structure (as histone octamers surrounded by approximately 200 base pairs of DNA) were proposed by Roger Kornberg. The role of the nucleosome as a general gene repressor was demonstrated by Lorch et al. in vitro and by Han and Grunstein in vivo.The nucleosome core particle consists of approximately 147 base pairs of DNA wrapped in 1.67 left-handed superhelical turns around a histone octamer consisting of 2 copies each of the core histones H2A, H2B, H3, and H4. Core particles are connected by stretches of ""linker DNA"", which can be up to about 80 bp long. Technically, a nucleosome is defined as the core particle plus one of these linker regions; however the word is often synonymous with the core particle. Genome-wide nucleosome positioning maps are now available for many model organisms including mouse liver and brain.Linker histones such as H1 and its isoforms are involved in chromatin compaction and sit at the base of the nucleosome near the DNA entry and exit binding to the linker region of the DNA. Non-condensed nucleosomes without the linker histone resemble ""beads on a string of DNA"" under an electron microscope.In contrast to most eukaryotic cells, mature sperm cells largely use protamines to package their genomic DNA, most likely to achieve an even higher packaging ratio. Histone equivalents and a simplified chromatin structure have also been found in Archea, suggesting that eukaryotes are not the only organisms that use nucleosomes.