Cellular DNA Polymerases - DNA Replication and Human Disease
... Bacillus phage29. The similarity between these conserved regions is
most striking in their linear spatial arrangement on each pol polypeptide.
The regions are designated I-VI by their extent of similarity, with region
I being the most conserved (Wong et al. 1988). Delarue et al. designated
the t ...
... Bacillus phage
Catabolic Alanine Racemase from Salmonella typhimurium: DNA Sequence, Enzyme Purification, and Characterization.
... Physical and Kinetic Characterization. Comparison of the denatured (39 000) and native (50000) molecular weights clearly indicates that the active enzyme, as isolated, is monomeric. By use of a molecular weight of 39000, one PLP molecule was bound per enzyme monomer, as determined by the fluorometri ...
... Physical and Kinetic Characterization. Comparison of the denatured (39 000) and native (50000) molecular weights clearly indicates that the active enzyme, as isolated, is monomeric. By use of a molecular weight of 39000, one PLP molecule was bound per enzyme monomer, as determined by the fluorometri ...
Fluoroquinolones
... • Inhibition of DNA gyrase prevents the relaxation of positively supercoiled DNA that is required for normal transcription and replication • Inhibition of topoisomerase IV interferes with separation of replicated chromosomal DNA into the respective daughter cells during cell division ...
... • Inhibition of DNA gyrase prevents the relaxation of positively supercoiled DNA that is required for normal transcription and replication • Inhibition of topoisomerase IV interferes with separation of replicated chromosomal DNA into the respective daughter cells during cell division ...
Insights into Protein–DNA Interactions through Structure
... Protein–DNA interactions are crucial for many cellular processes. Now with the increased availability of structures of protein–DNA complexes, gaining deeper insights into the nature of protein–DNA interactions has become possible. Earlier, investigations have characterized the interface properties b ...
... Protein–DNA interactions are crucial for many cellular processes. Now with the increased availability of structures of protein–DNA complexes, gaining deeper insights into the nature of protein–DNA interactions has become possible. Earlier, investigations have characterized the interface properties b ...
Replication origin plasticity, Taylor-made: inhibition vs
... independently derived cell lines in which the amplicons (segments of amplified chromosomal DNA) had different structures. Surprisingly, one of these cell lines (474) initiated replication at several origins in addition to the previously mapped origin that was preferentially used in two other lines ( ...
... independently derived cell lines in which the amplicons (segments of amplified chromosomal DNA) had different structures. Surprisingly, one of these cell lines (474) initiated replication at several origins in addition to the previously mapped origin that was preferentially used in two other lines ( ...
number of fifty human tumours
... the same mean DNA content per chromosome as for normal cells) was calculated. In this calculation, allowance has been made for the apparent difference in DNA content between normal epithelial cells and cells of mesenchymal origin: using the present microspectrophotometric technique, uterine epitheli ...
... the same mean DNA content per chromosome as for normal cells) was calculated. In this calculation, allowance has been made for the apparent difference in DNA content between normal epithelial cells and cells of mesenchymal origin: using the present microspectrophotometric technique, uterine epitheli ...
Chapter 1: The Genetic Approach to Biology Questions for Chapter 1
... Nucleotides are joined by weak hydrogen bonds that can be separated by DNA polymerase or helicase Nucleotides (ATGC) are joined to strand at sulfur and phosphorus Strong covalent bonds occur along each strand (sulfur and phosporus) 2. Diversity of Genes There are four kinds of nucleotide (ATGC) wit ...
... Nucleotides are joined by weak hydrogen bonds that can be separated by DNA polymerase or helicase Nucleotides (ATGC) are joined to strand at sulfur and phosphorus Strong covalent bonds occur along each strand (sulfur and phosporus) 2. Diversity of Genes There are four kinds of nucleotide (ATGC) wit ...
Gene Section CDT1 (chromatin licensing and DNA replication factor 1)
... Cyclin-binding motif is the target for phosphorylation by cyclin A-dependent kinases, which results in the binding of Cdt1 to the F-box protein Skp2 and subsequent degradation. Interaction with geminin, a small regulatory protein active during S, G2, and M phases of the cell cycle, protects CDT1 fro ...
... Cyclin-binding motif is the target for phosphorylation by cyclin A-dependent kinases, which results in the binding of Cdt1 to the F-box protein Skp2 and subsequent degradation. Interaction with geminin, a small regulatory protein active during S, G2, and M phases of the cell cycle, protects CDT1 fro ...
Chapter 8 Human Chromosomes
... consequence is that each mitochondrion has between 2 to 10 identical copies of each mitochondrial chromosome (Figure 8-9). ...
... consequence is that each mitochondrion has between 2 to 10 identical copies of each mitochondrial chromosome (Figure 8-9). ...
Trawling DNA Databases for Partial Matches: What is the FBI Afraid
... WHAT IS THE FBI AFRAID OF? David H. Kaye * DNA evidence is often presented as the "gold standard"for forensic science. But this was not always the case. For years, eminent scientists complained that the estimates of the tiny frequencies of DNA types were unfounded. It took scores of research papers, ...
... WHAT IS THE FBI AFRAID OF? David H. Kaye * DNA evidence is often presented as the "gold standard"for forensic science. But this was not always the case. For years, eminent scientists complained that the estimates of the tiny frequencies of DNA types were unfounded. It took scores of research papers, ...
Sequence-Specific Deoxyribonucleic Acid (DNA) Recognition by
... Bombyx mori; Dr, Danio rerio; Ce, Caenorhabditis elegans; Rn, Rattus norvegicus. C, The upstream promoter region of the mouse inhibin-" subunit gene encompassing the SBS and the cAMP response element (CRE). Numbering is relative to the transcriptional start site. The 15-bp sequence used for the NMR ...
... Bombyx mori; Dr, Danio rerio; Ce, Caenorhabditis elegans; Rn, Rattus norvegicus. C, The upstream promoter region of the mouse inhibin-" subunit gene encompassing the SBS and the cAMP response element (CRE). Numbering is relative to the transcriptional start site. The 15-bp sequence used for the NMR ...
lecture 1 File
... transformation is a bacterial adaptation for DNA transfer between two cells through the intervening medium. The uptake of donor DNA and its recombinational incorporation into the recipient chromosome depends on the expression of numerous bacterial genes whose products direct this process. In general ...
... transformation is a bacterial adaptation for DNA transfer between two cells through the intervening medium. The uptake of donor DNA and its recombinational incorporation into the recipient chromosome depends on the expression of numerous bacterial genes whose products direct this process. In general ...
Chromatin Fibers Observed In Situ in Frozen Hydrated Sections
... with chromatin fibers, and, in general, features seen in the epoxy sections have a similar morphology to those present in the frozen material. When cells were fixed with glutaraldehyde after Hepes-NaC1 and then frozen, they appeared identical to the unfixed cells (not shown). The two species of echi ...
... with chromatin fibers, and, in general, features seen in the epoxy sections have a similar morphology to those present in the frozen material. When cells were fixed with glutaraldehyde after Hepes-NaC1 and then frozen, they appeared identical to the unfixed cells (not shown). The two species of echi ...
A new FISH protocol with increased sensitivity for
... both on and out of the nuclei (Morais-Cecilio et al., 1997). Table 1, which shows the percentage of labelled nuclei, gives an estimation of the hybridization efficiency, that is between 45% and 70% depending on the probe and on the material. Table 2 shows the distribution of the number of spots per nu ...
... both on and out of the nuclei (Morais-Cecilio et al., 1997). Table 1, which shows the percentage of labelled nuclei, gives an estimation of the hybridization efficiency, that is between 45% and 70% depending on the probe and on the material. Table 2 shows the distribution of the number of spots per nu ...
View poster
... genome sequencing (WGS) or targeted enrichment using exome or gene panels. Copy number variation (CNV) of genomic segments is a large category of structural variation and has been implicated in many Mendelian diseases and complex traits. The impact of CNVs on gene expression is not limited to only t ...
... genome sequencing (WGS) or targeted enrichment using exome or gene panels. Copy number variation (CNV) of genomic segments is a large category of structural variation and has been implicated in many Mendelian diseases and complex traits. The impact of CNVs on gene expression is not limited to only t ...
187-192. Control of transcription by Pontin and Reptin
... Another complex containing both Pontin and Reptin was purified from vertebrate cells and called Uri1 complex (41). Besides Pontin and Reptin, this complex contains components of the E3ubiquitin ligase SCFSkp2, Rbp5 (a protein previously shown to associate with RNA Polymerases I, II, and III), and se ...
... Another complex containing both Pontin and Reptin was purified from vertebrate cells and called Uri1 complex (41). Besides Pontin and Reptin, this complex contains components of the E3ubiquitin ligase SCFSkp2, Rbp5 (a protein previously shown to associate with RNA Polymerases I, II, and III), and se ...
A Sex Chromosome Rearrangement in a Human XX
... paternal X, the paternal Y, and a maternal X chromosome). Those pseudoautosomal loci distal to U7 would be present in only two copies (one from the paternal Y chromosome and one from a maternal X chromosome). We therefore determined the copy number of several pseudoautosomal loci in CONlOl. To obtai ...
... paternal X, the paternal Y, and a maternal X chromosome). Those pseudoautosomal loci distal to U7 would be present in only two copies (one from the paternal Y chromosome and one from a maternal X chromosome). We therefore determined the copy number of several pseudoautosomal loci in CONlOl. To obtai ...
HS-SCI-APB-Unit 3 -- Chapter 16- Molecular Basis
... protein in one batch of T2 and a radioactive isotope of phosphorus to tag DNA in a second batch. Because protein, but not DNA, contains sulfur, radioactive sulfur atoms were incorporated only into the protein of the phage. In a similar way, the atoms of radioactive phosphorus labeled only the DNA, n ...
... protein in one batch of T2 and a radioactive isotope of phosphorus to tag DNA in a second batch. Because protein, but not DNA, contains sulfur, radioactive sulfur atoms were incorporated only into the protein of the phage. In a similar way, the atoms of radioactive phosphorus labeled only the DNA, n ...
Circadian Regulator CLOCK Is a Histone Acetyltransferase
... proteins (Figure 1B). It is also noteworthy that CLOCK and ACTR share a number of other structural features outside of the carboxy-terminal glutamine-rich region. These include the highly conserved bHLH-PAS domain at the amino termini, a NRID (nuclear receptor interaction domain), as well as serine- ...
... proteins (Figure 1B). It is also noteworthy that CLOCK and ACTR share a number of other structural features outside of the carboxy-terminal glutamine-rich region. These include the highly conserved bHLH-PAS domain at the amino termini, a NRID (nuclear receptor interaction domain), as well as serine- ...
Oncomedicine Base Excision Repair Manipulation in Breast
... vs sporadic occurrences. It is widely accepted that genetic alterations increase susceptibility due to inherited heterozygous gene defect in BRCA1, TP53 and PTEN [1, 3, 25]. The supporting evidences substantiate that gene factors such as tumor suppressor genes associated with genome fidelity as BRCA ...
... vs sporadic occurrences. It is widely accepted that genetic alterations increase susceptibility due to inherited heterozygous gene defect in BRCA1, TP53 and PTEN [1, 3, 25]. The supporting evidences substantiate that gene factors such as tumor suppressor genes associated with genome fidelity as BRCA ...
Multifractal analysis of DNA sequences using a novel chaos
... two of them on the 1=f spectrum of DNA sequences [3]. By mapping the sequence onto a (1D) walk, Peng and others have built a kind of interface, whose statistics were used to probe the range of correlation of the sequences [4,5]. Linguistic features were claimed to have been found in noncoding DNA s ...
... two of them on the 1=f spectrum of DNA sequences [3]. By mapping the sequence onto a (1D) walk, Peng and others have built a kind of interface, whose statistics were used to probe the range of correlation of the sequences [4,5]. Linguistic features were claimed to have been found in noncoding DNA s ...
Genome Biology - Department of Computer Science and
... hierarchy, and in a number of additional features, indicating possible differences in their transcriptional regulation mechanisms. ...
... hierarchy, and in a number of additional features, indicating possible differences in their transcriptional regulation mechanisms. ...
Sample pages 2 PDF
... Put simply, horizontal transfer of genetic information allows bacteria to evolve in ways that vertical transmission of random mutations from mother to daughter cells will not facilitate. Therefore, the study of these genetic exchange mechanisms will reveal a set of strategies that bacteria use to ev ...
... Put simply, horizontal transfer of genetic information allows bacteria to evolve in ways that vertical transmission of random mutations from mother to daughter cells will not facilitate. Therefore, the study of these genetic exchange mechanisms will reveal a set of strategies that bacteria use to ev ...
Nucleosome
A nucleosome is a basic unit of DNA packaging in eukaryotes, consisting of a segment of DNA wound in sequence around eight histone protein cores. This structure is often compared to thread wrapped around a spool.Nucleosomes form the fundamental repeating units of eukaryotic chromatin, which is used to pack the large eukaryotic genomes into the nucleus while still ensuring appropriate access to it (in mammalian cells approximately 2 m of linear DNA have to be packed into a nucleus of roughly 10 µm diameter). Nucleosomes are folded through a series of successively higher order structures to eventually form a chromosome; this both compacts DNA and creates an added layer of regulatory control, which ensures correct gene expression. Nucleosomes are thought to carry epigenetically inherited information in the form of covalent modifications of their core histones.Nucleosomes were observed as particles in the electron microscope by Don and Ada Olins and their existence and structure (as histone octamers surrounded by approximately 200 base pairs of DNA) were proposed by Roger Kornberg. The role of the nucleosome as a general gene repressor was demonstrated by Lorch et al. in vitro and by Han and Grunstein in vivo.The nucleosome core particle consists of approximately 147 base pairs of DNA wrapped in 1.67 left-handed superhelical turns around a histone octamer consisting of 2 copies each of the core histones H2A, H2B, H3, and H4. Core particles are connected by stretches of ""linker DNA"", which can be up to about 80 bp long. Technically, a nucleosome is defined as the core particle plus one of these linker regions; however the word is often synonymous with the core particle. Genome-wide nucleosome positioning maps are now available for many model organisms including mouse liver and brain.Linker histones such as H1 and its isoforms are involved in chromatin compaction and sit at the base of the nucleosome near the DNA entry and exit binding to the linker region of the DNA. Non-condensed nucleosomes without the linker histone resemble ""beads on a string of DNA"" under an electron microscope.In contrast to most eukaryotic cells, mature sperm cells largely use protamines to package their genomic DNA, most likely to achieve an even higher packaging ratio. Histone equivalents and a simplified chromatin structure have also been found in Archea, suggesting that eukaryotes are not the only organisms that use nucleosomes.