Mutagenesis identifies the critical amino acid residues of human
... critical amino acid residues of EndoG and proposed the catalytic mechanism of EndoG. Methods: To identify the critical amino acid residues of human EndoG, we replaced the conserved histidine, asparagine, and arginine residues with alanine. The catalytic efficacies of Escherichia coliexpressed EndoG ...
... critical amino acid residues of EndoG and proposed the catalytic mechanism of EndoG. Methods: To identify the critical amino acid residues of human EndoG, we replaced the conserved histidine, asparagine, and arginine residues with alanine. The catalytic efficacies of Escherichia coliexpressed EndoG ...
p53 regulation and function in normal cells and tumors
... and chemical carcinogens. Mechanisms to protect the integrity of inherited genetic information from the consequences of such exposure include the pathway of the tumor suppressor p53 protein, a guardian of the genome. p53 is a DNA binding protein; attention has focused on its specific binding to a co ...
... and chemical carcinogens. Mechanisms to protect the integrity of inherited genetic information from the consequences of such exposure include the pathway of the tumor suppressor p53 protein, a guardian of the genome. p53 is a DNA binding protein; attention has focused on its specific binding to a co ...
Diversity of DNA methyltransferases that recognize asymmetric
... and structural studies are required to confirm the relevance of this dimerization. The extent of subunit contacts observed in the crystal structure of M.MboIIA suggested that the two molecules in the asymmetric unit represent a biologically relevant dimer. In M.AhdI, two copies of the AhdIS subunit ...
... and structural studies are required to confirm the relevance of this dimerization. The extent of subunit contacts observed in the crystal structure of M.MboIIA suggested that the two molecules in the asymmetric unit represent a biologically relevant dimer. In M.AhdI, two copies of the AhdIS subunit ...
Single Cell Analysis of RNA-mediated Histone H3.3 Transcription Site
... regulates H3.3 assembly at MyoD and myogenic regulatory sequences and is also required for differentiation (18). The diversity of RI H3.3 chromatin assembly factors and the incorporation of H3.3 into both active and silent loci indicate that H3.3 regulates a wide array of genomic events, but they do ...
... regulates H3.3 assembly at MyoD and myogenic regulatory sequences and is also required for differentiation (18). The diversity of RI H3.3 chromatin assembly factors and the incorporation of H3.3 into both active and silent loci indicate that H3.3 regulates a wide array of genomic events, but they do ...
DAPI: a DMA-Specific Fluorescent Probe
... RNA and proteins (Kapuscinski and Skoczylas 1977) and cellular homogenates (Brunk et al. 1979). The sensitivity of this method reaches 0.5 ng DNA/ml. The assay can not be performed in the presence of a n anionic detergent, such as sodium docasyl sulfate (SDS), which enhances the fluorescence of the ...
... RNA and proteins (Kapuscinski and Skoczylas 1977) and cellular homogenates (Brunk et al. 1979). The sensitivity of this method reaches 0.5 ng DNA/ml. The assay can not be performed in the presence of a n anionic detergent, such as sodium docasyl sulfate (SDS), which enhances the fluorescence of the ...
Biotechnology
... 1. Which of the following are important features for transcription? a. promoter b. RNA polymerase c. 5′and 3′UTRs d. ORF e. all of the above 2. For which of the following nitrogenous bases does DNA substitute thymine? a. uracil b. adenine c. guanine d. cytosine e. inosine 3. Which of the following ...
... 1. Which of the following are important features for transcription? a. promoter b. RNA polymerase c. 5′and 3′UTRs d. ORF e. all of the above 2. For which of the following nitrogenous bases does DNA substitute thymine? a. uracil b. adenine c. guanine d. cytosine e. inosine 3. Which of the following ...
Recruitment of Drosophila Polycomb Group proteins to
... The Mini-Fab construct (93bp) was also unable to induce variegation and PSS, or to recruit PcG proteins (Fig. 1a and Ref. 2). This narrowed down the additional PcG recruiter DNA motifs to three blocks of Ab-Fab DNA that are located outside Mini-Fab and do not represent PHO, GAF or Zeste consensus mo ...
... The Mini-Fab construct (93bp) was also unable to induce variegation and PSS, or to recruit PcG proteins (Fig. 1a and Ref. 2). This narrowed down the additional PcG recruiter DNA motifs to three blocks of Ab-Fab DNA that are located outside Mini-Fab and do not represent PHO, GAF or Zeste consensus mo ...
Magnusiomyces capitatus (de Hoog et al.) de Hoog et Smith
... 1. Fungal genomic DNA is provided in a dried form. Store at +2°C to 8°C upon receipt. Store at 20°C if stored for more than 6 months). Note: Do not store in freezers with a defrost cycle. This will expose the product to increased temperatures. 2. Concentration by PicoGreen® measurement was foun ...
... 1. Fungal genomic DNA is provided in a dried form. Store at +2°C to 8°C upon receipt. Store at 20°C if stored for more than 6 months). Note: Do not store in freezers with a defrost cycle. This will expose the product to increased temperatures. 2. Concentration by PicoGreen® measurement was foun ...
Comparison of DNA Replication in Cells from Prokarya and Eukarya
... recently been characterized, and in addition to the initiator proteinbinding sites that are analogous to the DnaA initiator protein-binding sites, within the E. coli OriC replicator, Cori contains essential transcriptional promoter elements that are only active in the stalked cell (Marczynski et al. ...
... recently been characterized, and in addition to the initiator proteinbinding sites that are analogous to the DnaA initiator protein-binding sites, within the E. coli OriC replicator, Cori contains essential transcriptional promoter elements that are only active in the stalked cell (Marczynski et al. ...
Major City Chiefs Position Paper on Sworn vs
... than a written test. Forensic providers must provide robust and documented training programs to qualify forensic scientists. These programs must consist of an appropriate amount of training and education in the specific scientific discipline, practical application of the techniques, testimony skills ...
... than a written test. Forensic providers must provide robust and documented training programs to qualify forensic scientists. These programs must consist of an appropriate amount of training and education in the specific scientific discipline, practical application of the techniques, testimony skills ...
A DNA Polymerase ε Mutant That Specifically Causes 1
... Screen for A12 homonucleotide run mutators: The mutagenized plasmid DNA was digested with AgeI or PimAI and transformed into cells, which were plated to uracil drop-out media. After 3 days of growth at 30⬚ colonies were replica plated to uracil and to lysine drop-out media. After 3 days of growth, c ...
... Screen for A12 homonucleotide run mutators: The mutagenized plasmid DNA was digested with AgeI or PimAI and transformed into cells, which were plated to uracil drop-out media. After 3 days of growth at 30⬚ colonies were replica plated to uracil and to lysine drop-out media. After 3 days of growth, c ...
Raven/Johnson Biology 8e Chapter 17 Answers 1. A recombinant
... d. a heat-resistant DNA polymerase The correct answer is d— A. Answer a is incorrect. Restriction endonucleases are used to break up DNA. They are not part of the polymerase chain reaction. The correct answer is d— B. Answer b is incorrect. PCR generates DNA fragments, not RNA. The correct answer is ...
... d. a heat-resistant DNA polymerase The correct answer is d— A. Answer a is incorrect. Restriction endonucleases are used to break up DNA. They are not part of the polymerase chain reaction. The correct answer is d— B. Answer b is incorrect. PCR generates DNA fragments, not RNA. The correct answer is ...
Protein–DNA Interactions: Amino Acid Conservation and the Effects
... where binding is independent of DNA sequence; (ii) highly specific, where binding is specific and all members of the family target the same DNA sequence; and (iii) multi-specific, where binding is also specific, but individual family members target different DNA sequences. Overall, protein residues ...
... where binding is independent of DNA sequence; (ii) highly specific, where binding is specific and all members of the family target the same DNA sequence; and (iii) multi-specific, where binding is also specific, but individual family members target different DNA sequences. Overall, protein residues ...
Amplification and partial sequencing of Ixodes Scapularis Shaker
... The National Institute of Allergy and Infectious Diseases has begun funding the DNA sequencing of the deer tick genome in hopes of understanding the role ticks play in passing pathogens to humans that cause lyme disease, rocky mountain spotted fever and tularemia (9). The research project undertaken ...
... The National Institute of Allergy and Infectious Diseases has begun funding the DNA sequencing of the deer tick genome in hopes of understanding the role ticks play in passing pathogens to humans that cause lyme disease, rocky mountain spotted fever and tularemia (9). The research project undertaken ...
PDF
... evident in the confocal images in Fig. 2A. Furthermore, on immunoblots of protein extracts from third instar larval salivary glands, H3S10ph labeling was greatly reduced in JIL-1 null mutant backgrounds confirming that the antibodies recognized the H3S10ph epitope. However, it should be noted that t ...
... evident in the confocal images in Fig. 2A. Furthermore, on immunoblots of protein extracts from third instar larval salivary glands, H3S10ph labeling was greatly reduced in JIL-1 null mutant backgrounds confirming that the antibodies recognized the H3S10ph epitope. However, it should be noted that t ...
Trawling DNA Databases For Partial Matches: What Is The FBI
... WHAT IS THE FBI AFRAID OF? David H. Kaye * DNA evidence is often presented as the “gold standard” for forensic science. But this was not always the case. For years, eminent scientists complained that the estimates of the tiny frequencies of DNA types were unfounded. It took scores of research papers ...
... WHAT IS THE FBI AFRAID OF? David H. Kaye * DNA evidence is often presented as the “gold standard” for forensic science. But this was not always the case. For years, eminent scientists complained that the estimates of the tiny frequencies of DNA types were unfounded. It took scores of research papers ...
Thermodynamic analysis of DNA binding by a Bacillus single
... ssDNA-binding domain to the functional form. The eukaryotic RPA is composed of three subunits (RPA70, RPA32, and RPA14) and functions as a heterotrimer through the use of four ssDNA-binding domains [2,3,18]. Unlike E. coli SSB, single stranded DNA binding protein from bacteriophage T4, the gene 32 p ...
... ssDNA-binding domain to the functional form. The eukaryotic RPA is composed of three subunits (RPA70, RPA32, and RPA14) and functions as a heterotrimer through the use of four ssDNA-binding domains [2,3,18]. Unlike E. coli SSB, single stranded DNA binding protein from bacteriophage T4, the gene 32 p ...
The Two Faces of Higher Eukaryotic DNA Replication Origins
... Earlier this year, Vaughn et al. (1990) described convincing evidence for the surprising conclusion that the DNA replication origin located downstream of the dihydrofolate reductase (DHFR) gene in Chinese hamster cells consists of a broad initiation zone extending over 26 kb. However, the data prese ...
... Earlier this year, Vaughn et al. (1990) described convincing evidence for the surprising conclusion that the DNA replication origin located downstream of the dihydrofolate reductase (DHFR) gene in Chinese hamster cells consists of a broad initiation zone extending over 26 kb. However, the data prese ...
Automated Constraint-Based Nucleotide Sequence Selection for
... To simplify the design of our computational systems, we have developed a tool for constraint-based selection of nucleotide sequences. This tool incorporates domain knowledge that has proven to be important in our experimental process. However, we have also formulated a framework for systematically s ...
... To simplify the design of our computational systems, we have developed a tool for constraint-based selection of nucleotide sequences. This tool incorporates domain knowledge that has proven to be important in our experimental process. However, we have also formulated a framework for systematically s ...
Screening of RYR1 genotypes in swine population by a rapid and
... discriminate between different genotypes. A good reaction optimization and an appropriate assay design are crucial points that can increase the amplitude of the profile difference and make sequence discrimination easier. However, the primers used for HRM must generate short amplicons. According to t ...
... discriminate between different genotypes. A good reaction optimization and an appropriate assay design are crucial points that can increase the amplitude of the profile difference and make sequence discrimination easier. However, the primers used for HRM must generate short amplicons. According to t ...
Electron transfer from aromatic amino acids to guanine and adenine
... Similar redox properties of the natural nucleobases and aromatic amino acids make it possible for electron transfer (ET) to occur between these sites in protein–nucleic acid complexes. Using DFT calculations, we estimate the ET rate from aromatic amino acid X (X = Phe, His, Tyr and Trp) to radical c ...
... Similar redox properties of the natural nucleobases and aromatic amino acids make it possible for electron transfer (ET) to occur between these sites in protein–nucleic acid complexes. Using DFT calculations, we estimate the ET rate from aromatic amino acid X (X = Phe, His, Tyr and Trp) to radical c ...
Resolvase OsGEN1 Mediates DNA Repair by
... Yen1/GEN1 are canonical Holliday junction resolvases that belong to the RAD2/XPG family. In eukaryotes, such as budding yeast, mice, worms, and humans, Yen1/GEN1 work together with Mus81-Mms4/MUS81-EME1 and Slx1-Slx4/SLX1-SLX4 in DNA repair by homologous recombination to maintain genome stability. I ...
... Yen1/GEN1 are canonical Holliday junction resolvases that belong to the RAD2/XPG family. In eukaryotes, such as budding yeast, mice, worms, and humans, Yen1/GEN1 work together with Mus81-Mms4/MUS81-EME1 and Slx1-Slx4/SLX1-SLX4 in DNA repair by homologous recombination to maintain genome stability. I ...
CtrA mediates a DNA replication checkpoint that prevents cell
... elements are located between ±24 and +7. Together, the results of the promoter deletion experiments indicate that essential promoter elements are located downstream of ±24 and upstream of +11. This region contains sequences similar to those of promoters that are positively regulated by the response ...
... elements are located between ±24 and +7. Together, the results of the promoter deletion experiments indicate that essential promoter elements are located downstream of ±24 and upstream of +11. This region contains sequences similar to those of promoters that are positively regulated by the response ...
Nucleosome
A nucleosome is a basic unit of DNA packaging in eukaryotes, consisting of a segment of DNA wound in sequence around eight histone protein cores. This structure is often compared to thread wrapped around a spool.Nucleosomes form the fundamental repeating units of eukaryotic chromatin, which is used to pack the large eukaryotic genomes into the nucleus while still ensuring appropriate access to it (in mammalian cells approximately 2 m of linear DNA have to be packed into a nucleus of roughly 10 µm diameter). Nucleosomes are folded through a series of successively higher order structures to eventually form a chromosome; this both compacts DNA and creates an added layer of regulatory control, which ensures correct gene expression. Nucleosomes are thought to carry epigenetically inherited information in the form of covalent modifications of their core histones.Nucleosomes were observed as particles in the electron microscope by Don and Ada Olins and their existence and structure (as histone octamers surrounded by approximately 200 base pairs of DNA) were proposed by Roger Kornberg. The role of the nucleosome as a general gene repressor was demonstrated by Lorch et al. in vitro and by Han and Grunstein in vivo.The nucleosome core particle consists of approximately 147 base pairs of DNA wrapped in 1.67 left-handed superhelical turns around a histone octamer consisting of 2 copies each of the core histones H2A, H2B, H3, and H4. Core particles are connected by stretches of ""linker DNA"", which can be up to about 80 bp long. Technically, a nucleosome is defined as the core particle plus one of these linker regions; however the word is often synonymous with the core particle. Genome-wide nucleosome positioning maps are now available for many model organisms including mouse liver and brain.Linker histones such as H1 and its isoforms are involved in chromatin compaction and sit at the base of the nucleosome near the DNA entry and exit binding to the linker region of the DNA. Non-condensed nucleosomes without the linker histone resemble ""beads on a string of DNA"" under an electron microscope.In contrast to most eukaryotic cells, mature sperm cells largely use protamines to package their genomic DNA, most likely to achieve an even higher packaging ratio. Histone equivalents and a simplified chromatin structure have also been found in Archea, suggesting that eukaryotes are not the only organisms that use nucleosomes.