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Geminivirus Replication Origins Have a Modular
Geminivirus Replication Origins Have a Modular

... within the stem-loop sequence (Stenger et al., 1991). This possiblity is further supported by studies of African cassava mosaic virus (ACMV) and wheat dwarf virus (Etessami et al., 1989; Heyraud et al., 1993). Second, the intracellular DNAforms that accumulate during ACMV infection correspond to rol ...
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PDF

... of magnetic poles. Such modifications, however, often come at a price. Bigger beads, for example, generate greater drag and reduce, therefore, the temporal resolution of the experiment [24]. Likewise, the use of microfabricated devices [18] can produce forces in nN range due to a dramatic decrease i ...
Pearson science 10 Teaching Program 3–4 weeks Chapter 1 DNA
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... In this chapter, students will learn about DNA and be able to describe its role in controlling the characteristics of organisms. They will use models and diagrams to represent relationships between DNA, genes and chromosomes. They will be able to explain the role of meiosis and fertilisation in the ...
Spatial Relationship between Transcription Sites and Chromosome
Spatial Relationship between Transcription Sites and Chromosome

... mammals, one X chromosome is transcriptionally inactivated during early development, while the other remains active (Lyon, 1961; Gartler and Goldman, 1994; Rastan, 1994; Heard et al., 1997). The two X chromosome territories in somatic female cell nuclei provide an excellent model system to study the ...
A unique pattern of intrastrand anomalies in base
A unique pattern of intrastrand anomalies in base

... function in O.nova in Table 1 the leaders and trailers have average lengths of 299 bp (range, 82 to 1153) and 228 bp (range, 91 to 446), respectively. Forty-one molecules from Euplotes species (Table 2) serve as a comparison group. Although Euplotes is a hypotrich, it is very distantly related to th ...
The β-Globin LCR is Not Necessary for an Open Chromatin
The β-Globin LCR is Not Necessary for an Open Chromatin

... All five HSs form when chromosome 11 is transferred to ...
Accepted Version - CSIRO Research Publications Repository
Accepted Version - CSIRO Research Publications Repository

... phenotypic variation in organisms and is an important factor in natural variation. Gene expression regulatory networks are comprised of cis- and trans-acting factors, and differences in gene expression are attributable to genetic variation. In eukaryotes, the genome is compacted into chromatin, and ...
Mechanisms of Unidirectional Translocation & Unwinding
Mechanisms of Unidirectional Translocation & Unwinding

... blue) and Mg++ (green, space filling). The helicase conserved motifs are shown in the same color as in (B). The subunits are colored the same as in (A). Amino acid residue R522 is shown. ...
insulin history
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A novel gene encoding a 54 kDa polypeptide is

... samples collected from oilfields. Three of them have been identified as Rhodococcus sp. IMT35, Pseudomonas sp. IMT37 and Pseudomonas sp. IMT40. SDS-PAGE analysis of the membrane of Rhodococcus sp. IMT35 revealed the presence of at least four polypeptides induced by propane. Polyclonal antibody raise ...
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... a racA mutation do not produce an axial filament and frequently fail to trap DNA inside the forespore (BenYehuda et al., 2003a; Wu and Errington, 2003). Cytological experiments show that the product of racA (RacA) is concentrated at the extreme poles of the sporangium and also exhibits a general, no ...
Real-time Quantification of HER2/neu Gene Amplification by
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... PCR approaches published so far have used reference genes which are not localized on chromosome 17. Therefore it is not possible to distinguish whether a small region of the chromosome or the whole chromosome is amplified. But chromosome aneuploidy, including loss and gain of chromosome 17, is seen ...
DNA barcoding parasite organisms found in terrestrial
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... biodiversity at a study site (Monis and Andrews 2002). There is also further interest in DNA barcoding of parasite biodiversity because of its noninvasive nature and its capability for early detection of invasive species, non-native species which may offset the balance of a pre-established ecosystem ...
The WD40-Repeat Proteins NFC101 and NFC102
The WD40-Repeat Proteins NFC101 and NFC102

... produced by these regions. All direct nfc101/nfc102 targets showed histone modification patterns linked to active chromatin in nfc101/nfc102 downregulation lines. However, different mechanisms may be involved because NFC101/NFC102 proteins mediate HDAC recruitment at id1 and TE repeats but not at ZCN ...
Educator's Resource Guide 4226  Biology 1 s 4-5
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... will occur. Probability predicts the recombination of alleles: ▶ Of an allele pair, the probability of each allele in a gamete is ½, or 50 percent. ▶ When F1 hybrid individuals are crossed, the probability of • two recessive alleles is ¼. • two dominant alleles is ¼. • one dominant allele and one re ...
What Are Chromosomes?
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... Figure 4-34. A cyclic mechanism for nucleosome disruption and re-formation. According to this model, different chromatin remodeling complexes disrupt and re-form nucleosomes, although, in principle, the same complex might catalyze both reactions. The DNA-binding proteins could function in gene expre ...
foreign
foreign

... Pistil accepts right type pollen , pollen grain germinates to produce pollen tube that grows and reaches the ovary , male gametes enter the ovule through micropyle , one male gamete fuses with nucleus of egg cell to form diploid zygote , other male gamete fuses with two polar nuclei forming primary ...
Crystal structures of -[Ru(phen)2dppz]2+ 1 with oligonucleotides
Crystal structures of -[Ru(phen)2dppz]2+ 1 with oligonucleotides

... This self-complementary sequence crystallizes to give a symmetrical duplex with a stoichiometry of three cations of 1 per duplex, or 1.5 cations of 1 per decamer strand. All the nucleic acid strands are equivalent in the crystal lattice, with the packing shown in Figure 1a. The conformation of a sin ...
DNA - CS.Duke
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...  hemizygous is when there is only 1 member of a chromosome segment rather than the usual 2 ...
Identification and removal of colanic acid from plasmid DNA
Identification and removal of colanic acid from plasmid DNA

... solvents such as ethanol (EtOH) and by polyethylene glycol. Because polysaccharides are anionic, they co-purify with DNA by anion-exchange chromatography. The commercially available laboratory scale purification methods, including Qiagen, use anionexchange chromatography for at least one major step ...
The promiscuous primase
The promiscuous primase

... particular, the arrangement of metal coordinating acidic residues shows tight conservation within a region of b sheet. Both ORF904 and Pyrococcus enzymes have zinc-binding structural elements adjacent to the active centre of the enzyme. Surprisingly, however, these motifs are found in unrelated posi ...
- Wiley Online Library
- Wiley Online Library

... elements perform discrete functions which can be tested with isolated DNA fragments does not mean that these elements act alone in their native loci. In fact, several recent reports suggest that interactions between cis-regulatory elements are commonplace, and may play relevant roles for the functio ...
Aberrant replication timing induces defective chromosome
Aberrant replication timing induces defective chromosome

... sister chromatids, suggested that euchromatin may be more prone to defective condensation than heterochromatin. This could be explained if condensation defects were a consequence of aberrant DNA replication. For example, alterations in the replication timing of particular loci might lead to changes ...
Module 7 – Microbial Molecular Biology and Genetics
Module 7 – Microbial Molecular Biology and Genetics

... like transcription factors that can bind to specific sequences in double-stranded DNA usually make contacts to the sides of the bases exposed in the major groove. In a DNA double helix, each type of nucleobase on one strand normally interacts with just one type of nucleobase on the other strand. Thi ...
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Nucleosome



A nucleosome is a basic unit of DNA packaging in eukaryotes, consisting of a segment of DNA wound in sequence around eight histone protein cores. This structure is often compared to thread wrapped around a spool.Nucleosomes form the fundamental repeating units of eukaryotic chromatin, which is used to pack the large eukaryotic genomes into the nucleus while still ensuring appropriate access to it (in mammalian cells approximately 2 m of linear DNA have to be packed into a nucleus of roughly 10 µm diameter). Nucleosomes are folded through a series of successively higher order structures to eventually form a chromosome; this both compacts DNA and creates an added layer of regulatory control, which ensures correct gene expression. Nucleosomes are thought to carry epigenetically inherited information in the form of covalent modifications of their core histones.Nucleosomes were observed as particles in the electron microscope by Don and Ada Olins and their existence and structure (as histone octamers surrounded by approximately 200 base pairs of DNA) were proposed by Roger Kornberg. The role of the nucleosome as a general gene repressor was demonstrated by Lorch et al. in vitro and by Han and Grunstein in vivo.The nucleosome core particle consists of approximately 147 base pairs of DNA wrapped in 1.67 left-handed superhelical turns around a histone octamer consisting of 2 copies each of the core histones H2A, H2B, H3, and H4. Core particles are connected by stretches of ""linker DNA"", which can be up to about 80 bp long. Technically, a nucleosome is defined as the core particle plus one of these linker regions; however the word is often synonymous with the core particle. Genome-wide nucleosome positioning maps are now available for many model organisms including mouse liver and brain.Linker histones such as H1 and its isoforms are involved in chromatin compaction and sit at the base of the nucleosome near the DNA entry and exit binding to the linker region of the DNA. Non-condensed nucleosomes without the linker histone resemble ""beads on a string of DNA"" under an electron microscope.In contrast to most eukaryotic cells, mature sperm cells largely use protamines to package their genomic DNA, most likely to achieve an even higher packaging ratio. Histone equivalents and a simplified chromatin structure have also been found in Archea, suggesting that eukaryotes are not the only organisms that use nucleosomes.
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