RT2 Profiler PCR Arrays: Pathway-focused gene
... This format allows for the characterization of multiple biological ...
... This format allows for the characterization of multiple biological ...
The slime strain was grown in Nelson B medium... for 22 hours. HCH stereoisomers were dissolved in 83% ethanol...
... The slime strain was grown in Nelson B medium in submerged culture (100 rpm at 27°C for 22 hours. HCH stereoisomers were dissolved in 83% ethanol (1.0 mg.ml) and were added to the medium between 0-8 ug/ml. Ethanol in <0.6% concentration does not influence the growth of the strain and MIPS synthesis ...
... The slime strain was grown in Nelson B medium in submerged culture (100 rpm at 27°C for 22 hours. HCH stereoisomers were dissolved in 83% ethanol (1.0 mg.ml) and were added to the medium between 0-8 ug/ml. Ethanol in <0.6% concentration does not influence the growth of the strain and MIPS synthesis ...
Efficient expression of ribozyme and reduction of stromelysin mRNA
... MMPs normally play an important role in embryogenesis, wound healing, and tissue remodeling ...
... MMPs normally play an important role in embryogenesis, wound healing, and tissue remodeling ...
The Interplay of Temperature and Genotype on Patterns
... ABSTRACT Alternative splicing is the highly regulated process of variation in the removal of introns from premessenger-RNA transcripts. The consequences of alternative splicing on the phenotype are well documented, but the impact of the environment on alternative splicing is not yet clear. We studie ...
... ABSTRACT Alternative splicing is the highly regulated process of variation in the removal of introns from premessenger-RNA transcripts. The consequences of alternative splicing on the phenotype are well documented, but the impact of the environment on alternative splicing is not yet clear. We studie ...
Neonatal Hyperbilirubinemia and Organic Anion Transporting
... for confirmation. Nonphysiological hyperbilirubinemia was defined as having 1 mg/dL of serum total bilirubin above the 95th percentile value (high-risk zone).17 All newborns with hyperbilirubinemia were investigated for the following reasons: congenital anomalies, increased hepatic enzyme levels, ...
... for confirmation. Nonphysiological hyperbilirubinemia was defined as having 1 mg/dL of serum total bilirubin above the 95th percentile value (high-risk zone).17 All newborns with hyperbilirubinemia were investigated for the following reasons: congenital anomalies, increased hepatic enzyme levels, ...
Identification of Full and Partial Class Relevant Genes
... classification problems. Signal-to-noise ratio [1], t-test [2], between-groups to within-groups ratio [3], support vector machine (SVM) based on recursive feature elimination method [4], and least squares bound method [5], etc. are among those applicable mainly to two-class problems. Microarray data ...
... classification problems. Signal-to-noise ratio [1], t-test [2], between-groups to within-groups ratio [3], support vector machine (SVM) based on recursive feature elimination method [4], and least squares bound method [5], etc. are among those applicable mainly to two-class problems. Microarray data ...
YEAST GENETICS AND MOLECULAR BIOLOGY
... There is unfortunately no real text book on yeast genetics and molecular biology Genetic Techniques for Biological Research by Corinne Michels gives a brief overview on yeast genetics and summarises genetic approaches Yeast Gene Analysis by Brown and Tuite is a book about methods There are excellent ...
... There is unfortunately no real text book on yeast genetics and molecular biology Genetic Techniques for Biological Research by Corinne Michels gives a brief overview on yeast genetics and summarises genetic approaches Yeast Gene Analysis by Brown and Tuite is a book about methods There are excellent ...
Highly specific radioimmunoassay for human insulin
... both in the circulation (6, 7) and in human islets (8). In the beta cell and in the circulation, it is generally accepted that the major conversion intermediates found are des 31,32 and des 64,65 proinsulins and not split proinsulins (9), because of the rapid cleavage of the newly exposed C-terminal ...
... both in the circulation (6, 7) and in human islets (8). In the beta cell and in the circulation, it is generally accepted that the major conversion intermediates found are des 31,32 and des 64,65 proinsulins and not split proinsulins (9), because of the rapid cleavage of the newly exposed C-terminal ...
Genetics of Hemophilia
... hemophilia should have their factor (VIII or IX) levels checked to ensure they have adequate levels for normal blood clotting. See the “Hemophilia Carrier Fact Sheet” for additional information regarding women who are carriers or at risk to be carriers of hemophilia. »» We encourage our patients and ...
... hemophilia should have their factor (VIII or IX) levels checked to ensure they have adequate levels for normal blood clotting. See the “Hemophilia Carrier Fact Sheet” for additional information regarding women who are carriers or at risk to be carriers of hemophilia. »» We encourage our patients and ...
Messenger RNA reprogramming by spliceosome-mediated
... exon (iv). In addition to the trans-splicing domain, PTMs include a binding domain responsible for specific targeting of the PTM and a coding domain that includes the new or modified genetic information that will reprogram the target (Figure 6). A more extensive discussion of PTM structure by Garcia ...
... exon (iv). In addition to the trans-splicing domain, PTMs include a binding domain responsible for specific targeting of the PTM and a coding domain that includes the new or modified genetic information that will reprogram the target (Figure 6). A more extensive discussion of PTM structure by Garcia ...
Transgenic Plastids in Basic Research and Plant Biotechnology
... to effective inhibition of plastid protein biosynthesis by spectinomycin (an aminoglycoside antibiotic speci®cally blocking translation on prokaryotic-type 70 S ribosomes). A successfully transformed chloroplast expresses the spectinomycin-resistance gene, aadA, thus allowing for continued cell and ...
... to effective inhibition of plastid protein biosynthesis by spectinomycin (an aminoglycoside antibiotic speci®cally blocking translation on prokaryotic-type 70 S ribosomes). A successfully transformed chloroplast expresses the spectinomycin-resistance gene, aadA, thus allowing for continued cell and ...
Chapter_012 - IHMC Public Cmaps (2)
... Epigenetic changes coupled with genetic changes and environmental-lifestyle factors cause the development of cancer ...
... Epigenetic changes coupled with genetic changes and environmental-lifestyle factors cause the development of cancer ...
1 Tuning of recombinant protein expression in Escherichia
... polymerases on DNA and of ribosomes on mRNA are known to depend on the transcription and translation initiation rates, which, in turn, are determined by the strengths of the promoter and of the ribosome binding site (RBS). For example, it was shown that the use of a strong RBS with a high initiation ...
... polymerases on DNA and of ribosomes on mRNA are known to depend on the transcription and translation initiation rates, which, in turn, are determined by the strengths of the promoter and of the ribosome binding site (RBS). For example, it was shown that the use of a strong RBS with a high initiation ...
Prep1.1 has essential genetic functions in hindbrain development
... form a subgroup of Meinox proteins that share ~80% overall amino acid sequence identity. By contrast, the Meis and Prep proteins share high amino acid sequence conservation only in specific domains (Fognani et al., 2002). An additional difference between Prep and Meis might lie in Hox proteins bindi ...
... form a subgroup of Meinox proteins that share ~80% overall amino acid sequence identity. By contrast, the Meis and Prep proteins share high amino acid sequence conservation only in specific domains (Fognani et al., 2002). An additional difference between Prep and Meis might lie in Hox proteins bindi ...
Prep1.1 has essential genetic functions in hindbrain development
... form a subgroup of Meinox proteins that share ~80% overall amino acid sequence identity. By contrast, the Meis and Prep proteins share high amino acid sequence conservation only in specific domains (Fognani et al., 2002). An additional difference between Prep and Meis might lie in Hox proteins bindi ...
... form a subgroup of Meinox proteins that share ~80% overall amino acid sequence identity. By contrast, the Meis and Prep proteins share high amino acid sequence conservation only in specific domains (Fognani et al., 2002). An additional difference between Prep and Meis might lie in Hox proteins bindi ...
Multiple Less Common Genetic Variants Explain the Association of
... lower HDL-C (7,8). TaqIB and HDL-C ⴝ high-density other variants of CETP have lipoprotein cholesterol been associated with reduced LD ⴝ linkage disequilibrium CETP activity. However, despite LDL-C ⴝ low-density the consistent association these lipoprotein cholesterol variants have with higher HDL-C ...
... lower HDL-C (7,8). TaqIB and HDL-C ⴝ high-density other variants of CETP have lipoprotein cholesterol been associated with reduced LD ⴝ linkage disequilibrium CETP activity. However, despite LDL-C ⴝ low-density the consistent association these lipoprotein cholesterol variants have with higher HDL-C ...
RESISTANCE AS A RESPONSE TO STRESS
... lelochemicals. Each of these stresses can affect insects differently, but all must be adequately dealt with for continued survival. Resistance has been defined as the development of the ability in a strain of an organism to tolerate doses of a toxicant which would prove lethal to the majority of ind ...
... lelochemicals. Each of these stresses can affect insects differently, but all must be adequately dealt with for continued survival. Resistance has been defined as the development of the ability in a strain of an organism to tolerate doses of a toxicant which would prove lethal to the majority of ind ...
Patterns of Inheritance
... c) Codominance: ¾ neither allele is dominant and both alleles are expressed in heterozygous individuals ¾ we will examine an example of codominance when we discuss human ABO blood types ...
... c) Codominance: ¾ neither allele is dominant and both alleles are expressed in heterozygous individuals ¾ we will examine an example of codominance when we discuss human ABO blood types ...
Unexpected Complexity of Poly(A)-Binding Protein Gene Families in
... phylogenetic tree from the alignable amino acid sequences. Bootstrap analysis (10,000 replicates) was run in branch-andbound mode. Expression analyses: Arabidopsis plants (cv. Columbia) were transformed (Clough and Bent 1998) with pDB414, made by subcloning the BclI fragment that includes ⵑ2000 bp o ...
... phylogenetic tree from the alignable amino acid sequences. Bootstrap analysis (10,000 replicates) was run in branch-andbound mode. Expression analyses: Arabidopsis plants (cv. Columbia) were transformed (Clough and Bent 1998) with pDB414, made by subcloning the BclI fragment that includes ⵑ2000 bp o ...
Association of Apolipoprotein E Alleles with Susceptibility to Age
... membrane deposits (lipids) and apolipo proteins (Apo E and B), it could be hypothesized that the variant allele affects the lipid metabolism and so contributes to the pathogenesis of this disease. In fact, since ε2, ε3, and ε4 alleles encode a segment in apo E receptor-binding region, they affect th ...
... membrane deposits (lipids) and apolipo proteins (Apo E and B), it could be hypothesized that the variant allele affects the lipid metabolism and so contributes to the pathogenesis of this disease. In fact, since ε2, ε3, and ε4 alleles encode a segment in apo E receptor-binding region, they affect th ...
Lampetra fluviatilis Neurotrophin Homolog, Descendant of a
... recommendations. cRNA probes were labeled with 32P-UTP (Amersham, Buckinghamshire, UK) by in vitro transcription and hybridized to 10 mg of total RNA. After hybridization the samples were treated with ribonuclease, and the protected cRNA fragments were analyzed on a 5% denaturing polyacrylamide gel ...
... recommendations. cRNA probes were labeled with 32P-UTP (Amersham, Buckinghamshire, UK) by in vitro transcription and hybridized to 10 mg of total RNA. After hybridization the samples were treated with ribonuclease, and the protected cRNA fragments were analyzed on a 5% denaturing polyacrylamide gel ...
PDF
... snail-Am is first detected as the disc-shaped Acropora embryo starts to shrink in circumference and the edges start to thicken, creating a concavity (Fig. 2A 18 h, B, C). At this stage, snail-Am expression is restricted to the cells on the concave side (Fig. 2F, J). Later, as the concavity deepens a ...
... snail-Am is first detected as the disc-shaped Acropora embryo starts to shrink in circumference and the edges start to thicken, creating a concavity (Fig. 2A 18 h, B, C). At this stage, snail-Am expression is restricted to the cells on the concave side (Fig. 2F, J). Later, as the concavity deepens a ...
BBSI Final Report
... site, in the middle of orf11. This mutation was detected after purified pMEF2 was tested by restriction with Pst I. The two pieces created by this cut will be inserted into pPV72 and the resultant plasmids used to test interference. The exact nature of this mutation will have to be determined using ...
... site, in the middle of orf11. This mutation was detected after purified pMEF2 was tested by restriction with Pst I. The two pieces created by this cut will be inserted into pPV72 and the resultant plasmids used to test interference. The exact nature of this mutation will have to be determined using ...
WOX4 - Durham Research Online
... radial axis of the stem. These divisions result in highly distinctive cell files that are characteristic of the xylem in most dicots. The cell files that are generated are also essential for maintaining the spatial separation of xylem and phloem (Caño-Delgado et al., 2010). These properties make vas ...
... radial axis of the stem. These divisions result in highly distinctive cell files that are characteristic of the xylem in most dicots. The cell files that are generated are also essential for maintaining the spatial separation of xylem and phloem (Caño-Delgado et al., 2010). These properties make vas ...
The dystrophin / utrophin homologues in Drosophila and in sea urchin
... sequence identity with dystrophin were characterized. Utrophin (DRP1) is encoded by an autosomal gene and consists of all four domains of dystrophin. At the amino acid level the two proteins show ,51% identity. The exon/intron structures of the two genes are also very similar (Love et al., 1989; Pea ...
... sequence identity with dystrophin were characterized. Utrophin (DRP1) is encoded by an autosomal gene and consists of all four domains of dystrophin. At the amino acid level the two proteins show ,51% identity. The exon/intron structures of the two genes are also very similar (Love et al., 1989; Pea ...