Repressing Integrase attachment site operation
... between two heterotypic DNA “attachment” sites (attP and attB) [4]. Without the presence of a cognate directionality factor, the integrase will not allow the reaction to proceed backwards, between the recombination products of those sites (attL and attR) [5]. Serine integrases are also notable for n ...
... between two heterotypic DNA “attachment” sites (attP and attB) [4]. Without the presence of a cognate directionality factor, the integrase will not allow the reaction to proceed backwards, between the recombination products of those sites (attL and attR) [5]. Serine integrases are also notable for n ...
Isolation and characterization of an RNA that binds with high affinity
... from the CMV early promoter was greatly reduced by the addition of 100 pmole of authentic TAR RNA (lanes 3 and 4). Quantification of the results of four independent transcription experiments in the presence of TAR RNA revealed that transcription was inhibited by 60-70% (F i g . 4B). By contrast, aft ...
... from the CMV early promoter was greatly reduced by the addition of 100 pmole of authentic TAR RNA (lanes 3 and 4). Quantification of the results of four independent transcription experiments in the presence of TAR RNA revealed that transcription was inhibited by 60-70% (F i g . 4B). By contrast, aft ...
What is a protein
... Cell structure – Cellular material is 80% composed of proteins Cellular processes – hormones and enzymes Membrane channel proteins for transport of materials in and out of the cells Cell identification To initiate responses. ...
... Cell structure – Cellular material is 80% composed of proteins Cellular processes – hormones and enzymes Membrane channel proteins for transport of materials in and out of the cells Cell identification To initiate responses. ...
C2005/F2401 Key to Exam #3
... determines which strand is transcribed. If the enzyme Z gene can be transcribed (successfully) in either orientation, then the fragment itself must contain the promoter of the Z gene as well as the coding region for the enzyme. Therefore, the gene is always in the same orientation to its promoter no ...
... determines which strand is transcribed. If the enzyme Z gene can be transcribed (successfully) in either orientation, then the fragment itself must contain the promoter of the Z gene as well as the coding region for the enzyme. Therefore, the gene is always in the same orientation to its promoter no ...
TruSeq™ Sample Preparation Best Practices and Troubleshooting
... (dsDNA). These can be viscous and not evenly dispersed, resulting in aliquot measurements that are not representative of the true concentration of the solution. ` To minimize pipetting errors, especially with small volume enzyme additions, prepare the reagents for multiple samples simultaneously. ...
... (dsDNA). These can be viscous and not evenly dispersed, resulting in aliquot measurements that are not representative of the true concentration of the solution. ` To minimize pipetting errors, especially with small volume enzyme additions, prepare the reagents for multiple samples simultaneously. ...
Antisense derivatives of U7 small nuclear RNA as
... For any cellular assay system that is amenable to DNA transfection by means of lipid agents or calcium phosphate precipitation (e.g. splicing reporter genes in HeLa cells or in other easily transfectable cell types), we directly introduce the pSP64-derived U7 Sm OPT plasmid by this route. The splic ...
... For any cellular assay system that is amenable to DNA transfection by means of lipid agents or calcium phosphate precipitation (e.g. splicing reporter genes in HeLa cells or in other easily transfectable cell types), we directly introduce the pSP64-derived U7 Sm OPT plasmid by this route. The splic ...
Transcription Coactivator Family Proteins
... location and order of the variously indicated transcription factor-binding sites is only diagrammatic and not indicative as being typical of all eukaryotic mRNA genes. There exists a vast array of different transcription factors that regulate the transcription of all 3 classes of eukaryotic gene enc ...
... location and order of the variously indicated transcription factor-binding sites is only diagrammatic and not indicative as being typical of all eukaryotic mRNA genes. There exists a vast array of different transcription factors that regulate the transcription of all 3 classes of eukaryotic gene enc ...
PSI Notebook in PDF format
... trillions of copies are made from one original cell. But when cells reproduce, they must replicate (or copy) their DNA. The structure of DNA reveals how trillions of copies of the DNA in one of your cells can be made, and be almost exactly the same each time. ...
... trillions of copies are made from one original cell. But when cells reproduce, they must replicate (or copy) their DNA. The structure of DNA reveals how trillions of copies of the DNA in one of your cells can be made, and be almost exactly the same each time. ...
B Supplementary Figure 2
... respectively. FLK is a positive regulator of flowering via suppressing the expression of another flowering gene FLC, while the role of FLC is to repress flowering. (MiHye Lim et al 2004, Scott D. Michaels and Richard M. Amasino 1999). Actin (ACT2) is used as the internal control, so each tissue’s tr ...
... respectively. FLK is a positive regulator of flowering via suppressing the expression of another flowering gene FLC, while the role of FLC is to repress flowering. (MiHye Lim et al 2004, Scott D. Michaels and Richard M. Amasino 1999). Actin (ACT2) is used as the internal control, so each tissue’s tr ...
Soma-germline asymmetry in the distributions of
... division, however, we observed two distinct distribution patterns. A subset of maternal RNAs (8/14) continued to show a uniform distribution in all cells. We refer to these RNAs as class I maternal RNAs. A second class of maternal RNAs in our survey (6/14 ; designated class II maternal RNAs) showed ...
... division, however, we observed two distinct distribution patterns. A subset of maternal RNAs (8/14) continued to show a uniform distribution in all cells. We refer to these RNAs as class I maternal RNAs. A second class of maternal RNAs in our survey (6/14 ; designated class II maternal RNAs) showed ...
Paper
... Li et al. (Research Articles, 1 July 2011, p. 53; published online 19 May 2011) reported more than 10,000 mismatches between messenger RNA and DNA sequences from the same individuals, which they attributed to previously unrecognized mechanisms of gene regulation. We found that at least 88% of these ...
... Li et al. (Research Articles, 1 July 2011, p. 53; published online 19 May 2011) reported more than 10,000 mismatches between messenger RNA and DNA sequences from the same individuals, which they attributed to previously unrecognized mechanisms of gene regulation. We found that at least 88% of these ...
Coordination of Genomic RNA Packaging with Viral Assembly in HIV-1
... require specific interactions with viral RNA, since replacement of the domain with dimerizing leucine zipper motif restores the assembly function [9], however following capture of gRNA it is likely that the viral genome is involved. In summary, upon entering the cytoplasm following transcription and ...
... require specific interactions with viral RNA, since replacement of the domain with dimerizing leucine zipper motif restores the assembly function [9], however following capture of gRNA it is likely that the viral genome is involved. In summary, upon entering the cytoplasm following transcription and ...
Ch. 17 PPT
... Working with the mold Neurospora crassa, George Beadle and Edward Tatum had isolated mutants requiring arginine in their growth medium and had shown genetically that these mutants fell into three classes, each defective in a different gene. From other considerations, they suspected that the metaboli ...
... Working with the mold Neurospora crassa, George Beadle and Edward Tatum had isolated mutants requiring arginine in their growth medium and had shown genetically that these mutants fell into three classes, each defective in a different gene. From other considerations, they suspected that the metaboli ...
video slide - Fayetteville State University
... Working with the mold Neurospora crassa, George Beadle and Edward Tatum had isolated mutants requiring arginine in their growth medium and had shown genetically that these mutants fell into three classes, each defective in a different gene. From other considerations, they suspected that the metaboli ...
... Working with the mold Neurospora crassa, George Beadle and Edward Tatum had isolated mutants requiring arginine in their growth medium and had shown genetically that these mutants fell into three classes, each defective in a different gene. From other considerations, they suspected that the metaboli ...
Chapter 17 - Gene to Protein
... Working with the mold Neurospora crassa, George Beadle and Edward Tatum had isolated mutants requiring arginine in their growth medium and had shown genetically that these mutants fell into three classes, each defective in a different gene. From other considerations, they suspected that the metaboli ...
... Working with the mold Neurospora crassa, George Beadle and Edward Tatum had isolated mutants requiring arginine in their growth medium and had shown genetically that these mutants fell into three classes, each defective in a different gene. From other considerations, they suspected that the metaboli ...
video slide - Biology at Mott
... The discovery of ribozymes rendered obsolete the belief that all biological catalysts were proteins ...
... The discovery of ribozymes rendered obsolete the belief that all biological catalysts were proteins ...
The use of glycogen and GlycoBlue reagent in Qubit DNA and RNA
... (glycogen covalently linked to a blue dye; Cat. No. AM9515) are reagents commonly used to facilitate nucleic acid precipitation without adding extraneous nucleic acids to the sample. This study was carried out to determine whether glycogen or GlycoBlue Coprecipitant affects the accuracy of nucleic a ...
... (glycogen covalently linked to a blue dye; Cat. No. AM9515) are reagents commonly used to facilitate nucleic acid precipitation without adding extraneous nucleic acids to the sample. This study was carried out to determine whether glycogen or GlycoBlue Coprecipitant affects the accuracy of nucleic a ...
GeneMATRIX Universal DNA/RNA/Protein Purification Kit
... Note 1: This kit is designed for isolation of genomic DNA, total RNA, and total protein simultaneously from a single biological sample. Note 2: The kit is designed to purify DNA/RNA/Protein from a bacteria, tissue, plant, yeast or cell culture. Note 3: DNA binding capacity is 20 µg per spin-column. ...
... Note 1: This kit is designed for isolation of genomic DNA, total RNA, and total protein simultaneously from a single biological sample. Note 2: The kit is designed to purify DNA/RNA/Protein from a bacteria, tissue, plant, yeast or cell culture. Note 3: DNA binding capacity is 20 µg per spin-column. ...
Transcription and RNA processing
... nascent transcript. The C-terminal domain (CTD) of the largest subunit is then phosphorylated (P) at specific serine residues. Such modification is the signal for binding of the capping enzyme to the CTD and capping (blue) if the nascent RNA. ...
... nascent transcript. The C-terminal domain (CTD) of the largest subunit is then phosphorylated (P) at specific serine residues. Such modification is the signal for binding of the capping enzyme to the CTD and capping (blue) if the nascent RNA. ...
Interaction of Sesbania Mosaic Virus Movement Protein
... mostly confined to a single cell. Infection spreads to adjacent cells with the help of viral encoded specialised proteins, called movement proteins (MPs) [1]. The cell to cell movement of viruses is a complex and dynamic process which involves functional contribution from many proteins of viral and ...
... mostly confined to a single cell. Infection spreads to adjacent cells with the help of viral encoded specialised proteins, called movement proteins (MPs) [1]. The cell to cell movement of viruses is a complex and dynamic process which involves functional contribution from many proteins of viral and ...
Testing Gene Expression by Reverse Transcriptase PCR (rt
... from various types of retrovirus species). Once the cDNA is made it can be used like any other DNA as a template in PCR reactions with primers for specific genes. After a given number of cycles, the more abundant transcripts from highly transcribed genes (now in the form of cDNA) will yield more pro ...
... from various types of retrovirus species). Once the cDNA is made it can be used like any other DNA as a template in PCR reactions with primers for specific genes. After a given number of cycles, the more abundant transcripts from highly transcribed genes (now in the form of cDNA) will yield more pro ...