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Chapter 23 Lecture PowerPoint
Chapter 23 Lecture PowerPoint

... Phage coat is made of protein Always has the same volume DNA is much denser than protein More DNA in phage, denser phage Extra DNAs that can inactivate a gene by inserting into the gene were the first transposons discovered in bacteria • These transposons are called insertion sequences (ISs) ...
RNA/DNA catalysts
RNA/DNA catalysts

... Know about transesterification & cleavage reactions Know four types of natural catalytic RNAs (group I introns, group II introns, RNase P, small self-cleaving), what reactions they perform, know basics of their secondary and tertiary structure, requirements for cofactors/metals/proteins/ATP Know det ...
What is BIOLOGY?
What is BIOLOGY?

... Which 4 MACROMOLECULES are important to living things? (carbohydrates, lipids, proteins, and nucleic acids) What subunits are used to make these? Be able to ID these in a picture and give examples. ...
overview
overview

... structure (both strands) ...
Nucleic Acid Isolation System
Nucleic Acid Isolation System

... Cartridge ...
Answers to End-of-Chapter Questions – Brooker et al ARIS site
Answers to End-of-Chapter Questions – Brooker et al ARIS site

SB2a Build DNA using the Nucleotides Then Print
SB2a Build DNA using the Nucleotides Then Print

... The scissors below represent the enzyme called DNA helicase. It is responsible for cutting the DNA molecule in half by breaking the hydrogen bonds between the bases so that transcription can occur. Copy and paste the nucleotides from a previous slide to show how transcription occurs. RNA nucleotide ...
Extended Materials and Methods
Extended Materials and Methods

... RT-PCR and qPCR detection of messenger and small RNAs Preparation of the cDNA libraries from the total RNAs isolated from multiple tissues and sorted SMCs (pooled from 4 mice) obtained by FACS from smDicer-/-;Cre-GFP/+ or the WT control mice, as well as qPCR analysis on cDNAs, were performed as prev ...
Gene expression (central dogma)
Gene expression (central dogma)

... A DNA molecule consists of functional units called genes. Each gene provides instructions for a functional product (a molecule needed to perform a job in the cell). In many cases, the functional product of a gene is a protein. The functional products of most known genes are proteins, or, more accura ...
Gene Section AF15q14 (ALL1 fused gene from 15q14) in Oncology and Haematology
Gene Section AF15q14 (ALL1 fused gene from 15q14) in Oncology and Haematology

... t(11;15)(q23;q14)/acute non lymphocytic leukemia (ANLL) --> MLL/AF15q14 ...
Protein Synthesis Notes
Protein Synthesis Notes

... Gene mutations: a change in the sequence of nucleotides within a gene. When do mutations occur? - most often during DNA replication. ...
GLP 019 - University of Newcastle
GLP 019 - University of Newcastle

... Risk Assessment: . This Risk Assessment is to be used as a general guide and as such, cannot accommodate all the varying factors that may be encountered when using this equipment. Therefore, personnel are requested to conduct their own Risk Assessment before using this equipment to include any extra ...
a instructions to the candidates
a instructions to the candidates

... characteristics of histones is FALSE? 1. They are highly basic proteins found in the nucleus 2. They form non-covalent bonds with eukaryotic DNA called nucleosomes 3. They are very highly conserved proteins 4. They are associated with both prokaryotic and eukaryotic DNA 56. Which of the following is ...
1. Overview of Gene Expression Overview of Gene Expression Chapter 10B:
1. Overview of Gene Expression Overview of Gene Expression Chapter 10B:

... • translation also involves tRNAs, each of which is attached to 1 of the 20 amino acids (AAs) • ribosomes match the right tRNA (via the anticodon) with the right codon in the mRNA, then add its AA to the growing protein ...
Genotyping of Mice to Study Role of Krüppel
Genotyping of Mice to Study Role of Krüppel

... β-like genes, which could serve as targets for KLF2 binding ...
Supporting Material Binary gene induction and protein expression in
Supporting Material Binary gene induction and protein expression in

... The same as k1f and k1b, respectively. Since in the model switching from active to inactive promoter is TA-independent, the simulation results are largely insensitive to these two parameters. Eukaryotic protein-encoding genes are rarely transcribed by more than one RNA polymerase II at a time [6].  ...
File
File

... 6) In the absence of its inducer the lac operon remains repressed because of the binding of lac repressor to the _____. a) Catabolic gene activator protein. b) Operator. c) Antagonists. d) Klingons. e) Promoters. 7) Molecules that bind to a receptor and activate a signal transduction pathway are cal ...
Dr Ishtiaq Regulation of gene expression
Dr Ishtiaq Regulation of gene expression

protein synthesis
protein synthesis

... Here is a video that shows the process in detail It is created using simulations of the molecules involved ...
DNA and RNA Part 2 Protein Synthesis
DNA and RNA Part 2 Protein Synthesis

... functions ...
Transcription. (Ms. Shivani Bhagwat)
Transcription. (Ms. Shivani Bhagwat)

... information of RNA is translated into proteins Transcription (RNS synthesis) is carried out by RNA polymerases RNA polymerases are large complexes of proteins, not single enzymes Transcription is more complex in eukaryotes than in prokaryotes, but basic mechanisms are very similar in all living cell ...
Transcription in prokaryotes Elongation and termination
Transcription in prokaryotes Elongation and termination

... synthesized in the living cell. Because it is always possible that the 3’ end of the molecule has been generated by cleavage of the primary transcript, and therefore does not represent the actual site at which pol was terminated. The best identification of the terminator sites is provided by in vitr ...
Enzymes - year13bio
Enzymes - year13bio

... If DNA damage is irreparable or cells get too old they self destruct, called apoptosis. If damage occurs in either of the 2 genes mentioned above the cell will grow at an uncontrolled rate, or become effectively immortal. These cells cease to carry out normal functioning. If the damage is not too se ...
The Major Transitions in Evolution
The Major Transitions in Evolution

... RNAi is conserved in all major eukaryotic lineages, independent loss in many unicellular forms notwithstanding. • It appears most likely that LECA possessed relatively complex RNAi machinery. At a minimum, this primordial RNAi machinery consisted of an Argonaute-like protein, a Piwi-like protein, a ...
Chapter 25: Molecular Basis of Inheritance
Chapter 25: Molecular Basis of Inheritance

... Now RNA polymerase binds to the operator and brings about transcription of the genes that code for enzymes necessary to lactose metabolism. Structural genes code for enzymes of a metabolic pathway that are transcribed as a unit. A regulator gene codes for a repressor that can bind to the operator a ...
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RNA silencing

RNA silencing (associated with the concept of post-transcriptional gene silencing or RNA interference) refers to a family of gene silencing effects by which the expression of one or more genes is downregulated or entirely suppressed by non-coding RNAs, particularly small RNAs. It may also refer to the introduction of a synthetic antisense RNA molecule used in scientific experiments on gene expression. RNA silencing may also be defined as sequence-specific regulation of gene expression triggered by double-stranded RNA (dsRNA). RNA silencing mechanisms are highly conserved in most eukaryotes. The most common and well-studied example is RNA interference (RNAi), in which endogenously expressed microRNA (miRNA) or exogenously derived small interfering RNA (siRNA) induces the degradation of complementary messenger RNA. Other classes of small RNA have been identified, including piwi-interacting RNA (piRNA) and its subspecies repeat associated small interfering RNA (rasiRNA).
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