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PPT
PPT

Scenario: Phage Wars Identification of a Bacteriophage 80α
Scenario: Phage Wars Identification of a Bacteriophage 80α

... state within infected cells that allows replication and transmission of the phage genome along with that of the bacterial chromosome. This so-called prophage state is maintained by a phageencoded immunity repressor that prevents expression of the phage lytic genes. Bacterial cells carrying prophages ...
Gene Prediction Techniques - Computational Biology of RNA
Gene Prediction Techniques - Computational Biology of RNA

Table SI. Primers used for creation of the PHAC1co and
Table SI. Primers used for creation of the PHAC1co and

... (AT), HM440973 (UGTA1), EU552419 (CYP52M1) and unpublished results) which was determined using an online tool (Stothard, 2000). An SKL (TCTAAGCTG) peroxisomal target sequence (PTS) found in at least one peroxisomal protein of Candida bombicola (Van Bogaert et al., 2009a) was added to the 3’ terminus ...
Prioritizing curation of mouse genes by paucity of annotations and
Prioritizing curation of mouse genes by paucity of annotations and

... has become clear that ciliopathies are an underlying cause of numerous human diseases including some types of retinitis pigmentosa and polycystic kidney disease. Once thought to be restricted to a few cell types, it is now clear that primary cilia are found on almost all vertebrate cells and are cri ...
Gene Section IGL@ (Immunoglobulin Lambda) Atlas of Genetics and Cytogenetics
Gene Section IGL@ (Immunoglobulin Lambda) Atlas of Genetics and Cytogenetics

... to 11 IGLJ and 7 to 11 IGLC genes depending on the haplotypes, each IGLC gene being preceded by one IGLJ segment. Fifty-six to 57 genes belong to 11 subgroups, whereas 14 pseudogenes which are too divergent to be assigned to subgroups have been assigned to 3 clans. The most 5' IGLV genes occupy the ...
Leukaemia Section t(11;19)(q23;p13.3) MLL/ACER1 Atlas of Genetics and Cytogenetics in Oncology and Haematology
Leukaemia Section t(11;19)(q23;p13.3) MLL/ACER1 Atlas of Genetics and Cytogenetics in Oncology and Haematology

... nucleus, called MLL-N and MLL-C. The FYRN and FRYC domains of native MLL associate MLL-N and MLL-C in a stable complex; they form a multiprotein complex with transcription factor TFIID. MLL is a transcriptional regulatory factor. MLL can be associated with more than 30 proteins, including the core c ...
CHANGES IN THE EXPRESSION OF THREE COLD
CHANGES IN THE EXPRESSION OF THREE COLD

... 2005; Masny & Żurawicz 2007; Shokaeva 2008; Lukoševičiūtė 2013). However, only a few investigations on the genes regulating the cold-tolerance of strawberry were conducted (Owens et al. 2002; Schwab et al. 2009). In recent years, three key enzymes (chalcone synthase, CHS; flavonoid 3’-hydroxylase, F ...
Leukaemia Section t(2 14)(p13-16 32)
Leukaemia Section t(2 14)(p13-16 32)

... domain between zinc fingers 1 and 2 and an acidic domain between 3 and 4. 835 amino acids; 91197 Da, alternative splicing: 6 isoforms, sharing a common Nterminus. Originally named EV19 human homolog BCL11A; high level of conservation across a wide range of species; highly homologous to another gene ...
Less mastitis through targeted selective breeding Why a reduction of
Less mastitis through targeted selective breeding Why a reduction of

... Linkage and linkage disequilibrium analyses as well as genome-wide association analysis were used to identify several quantitative trait loci (QTL) affecting the resistance to clinical and subclinical mastitis. The sizes of these genomic regions were typically in the range of several million DNA bas ...
Facilitation of chromatin dynamics by SARs Craig M Hart and Ulrich
Facilitation of chromatin dynamics by SARs Craig M Hart and Ulrich

PDF file
PDF file

... performed using primers directed outward from the P element into the genomic DNA (Sullivan et al., 2000). The PCR product is then sequenced, and the non-P element DNA sequence (which should be Drosophila genomic DNA) is BLASTed against the Drosophila genome sequence (on BDGP: http://www.fruitfly.org ...
Improving Intergenic miRNA Target Genes Prediction
Improving Intergenic miRNA Target Genes Prediction

Transcriptional Repression of Hox Genes by C. elegans HP1/HPL
Transcriptional Repression of Hox Genes by C. elegans HP1/HPL

... Here, we investigate the role of HP1 and H1 in development using the nematode C. elegans as a model system. We focus on the underlying molecular mechanisms of gene co-regulation by H1 and HP1. We show that the loss of both HP1 and H1 alters the expression of a small subset of genes. C. elegans HP1 a ...
Presentazione di PowerPoint
Presentazione di PowerPoint

... crucial as not yet understood. Discovering their sequences and (hence) genomic locations is hard because they (mostly) small and poorly conserved over evolutionary time. In silico prediction methods are of high importance and very promising, but so far of little use. Currently, ncRNA are mostly disc ...
High-throughput reverse genetics: RNAi screens in
High-throughput reverse genetics: RNAi screens in

... were found, whereas only 70 genes with both mutations and clones were known before the screen. On chromosome III, 281 genes with a phenotype were found (86 previously known). In total, 12.9-13.9% of tested genes yielded a phenotype in the two screens, suggesting that the methods were about equally e ...
Fooyin University
Fooyin University

Antisense derivatives of U7 small nuclear RNA as
Antisense derivatives of U7 small nuclear RNA as

... well as the therapeutic time window, it may be desirable to induce and repress the expression of a U7 cassette at will or to express it specifically in certain tissues or cell types. In this respect it is important to note that the transcription of U snRNA genes is fundamentally different from that ...
Gene Expression Microarray Analysis of Archival FFPE Samples
Gene Expression Microarray Analysis of Archival FFPE Samples

... cancer patients.2 These studies utilized high quality RNA extracted from fresh frozen tumor tissue. Many laboratories are trying to develop methods to allow a similar degree of gene expression profiling using FFPE samples.3 FFPE samples represent the largest source of archival biological material av ...
Body maps on the human genome | SpringerLink
Body maps on the human genome | SpringerLink

... A tissue’s genes are not in general entirely exclusive to that tissue; shared genes tend to decrease contrast between tissues, and to blur any bodymapping. For each tissue, its set of maximally-selective genes was first drawn from results of Liang et al. [11]. This study included one of the largest ...
comparing quantitative trait loci and gene expression data
comparing quantitative trait loci and gene expression data

... adopted the embedded conversion tool in Expressionview (Fischer et al, 2003) to estimate physical distances from cM. The “smoothing window” technique used in Expressionview essentially applies the idea of piecewise regression. However at the edge of chromosomes and some middle places where possibly ...
S2 File.
S2 File.

... Fig A: Results of optical density measurement (top) and the crystal violet assay (bottom) for replicates (n = 3) of the wells that were harvested for RNA sequencing. Wells without NaCl addition were harvested after 1 day of incubation, while wells with NaCl addition were harvested after 2 days, to ...
Identification and functional analysis of novel genes
Identification and functional analysis of novel genes

... The analysis of the role of pleiotropic genes in germline development is rather difficult, but the RNAi technique provides an ideal tool since it enables the generation of a series of phenotypes of different strength. Using this approach, we were able to detect genes essential for germline developme ...
Combination of Neuro-Fuzzy Network Models with Biological
Combination of Neuro-Fuzzy Network Models with Biological

... pathways and bioprocesses. As a system, a GRN comprises of biomolecular components (genes, mRNA and proteins) which interact with each other. These interactions determine gene expression levels, that is, determine the rate of gene transcription to mRNA. In general, each mRNA molecule can be translat ...
Brooker Chapter 23
Brooker Chapter 23

... •Bicoid activates Krüppel; •Hunchback activates Krüppel at low concentrations and represses Krüppel at high ...
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Long non-coding RNA

Long non-coding RNAs (long ncRNAs, lncRNA) are non-protein coding transcripts longer than 200 nucleotides. This somewhat arbitrary limit distinguishes long ncRNAs from small regulatory RNAs such as microRNAs (miRNAs), short interfering RNAs (siRNAs), Piwi-interacting RNAs (piRNAs), small nucleolar RNAs (snoRNAs), and other short RNAs.
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