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ppt - Chair of Computational Biology
ppt - Chair of Computational Biology

... recognize different patterns and set different imprints in sperm and egg – how these imprint markers might find their targets: • tandem repeats – sequence not (well) conserved – like many DMRs – – are enriched in the CpG islands of imprinted genes – special DNA structure • sequence patterns (germ li ...
GENE 760 -‐ Problem Set #3
GENE 760 -‐ Problem Set #3

Transcription - Dr. Salah A. Martin
Transcription - Dr. Salah A. Martin

... humans may turn out to have only some 23 thousand genes, we probably make at least 10 times that number of different proteins. It is now estimated that 92-94% of our genes produce pre-mRNAs that are alternatively-spliced. There is evidence that the pattern of alternative splicing differs consistentl ...
Eukaryotic Transcription
Eukaryotic Transcription

... A scientist splices a eukaryotic promoter in front of a bacterial gene and inserts the gene in a bacterial chromosome. Would you expect the bacteria to transcribe the gene? The mouse genome includes one gene and two pseudogenes for cytoplasmic thymidine kinase. Pseudogenes are genes that have lost t ...
Document
Document

... MECP2 either in all tissues or selectively in brain was generated (Bird et al., 2001, Guy et al. 2001). • Cellular defects associated with MeCP2 deficiency in mouse CNS? • Behavioral defects in mice? ...
Gene Maps
Gene Maps

... Gene Linkage • Linked genes: genes located on the same chromosome that tend to be inherited together ...
Application of Biological Network
Application of Biological Network

... within the same disorder (red arrow) and the distribution of the expected number of interactions for the random control (blue). • Distribution of the tissue-homogeneity of a disorder (red). Random control (blue) with the same number of genes chosen randomly is shown for comparison. ...
Chapter 6
Chapter 6

... provided by hydrolysis of ATP. • The SWI/SNF, RSC, and NURF complexes all are very large; – there are some common subunits. ...
Course Competencies Template
Course Competencies Template

... Explaining how chemical modification of chromatin (DNA methylation and histone acetylation) affects DNA packing into chromatin and the availability of DNA for gene expression. ...
Determining mRNA with derived allele
Determining mRNA with derived allele

... alternatively spliced NMD transcript for ERAP2 (data not shown). We also find very limited expression of the full-length ERAP2 protein in HeLa protein extracts (Figure S4). These conflicting observations may be due to clonal differences among HeLa cell lines, which could have different ERAP2 genotyp ...
Promoters - Pennsylvania State University
Promoters - Pennsylvania State University

... at the start site for transcription. – beta-galactosidase: measure the hydrolysis of an analog of lactose that generates a colored, fluorescent or chemiluminescent product ...
Smooth Response Surface - University of British Columbia
Smooth Response Surface - University of British Columbia

...  Significantly expressed in at least 1 time point (5 time points in total) ->36 genes with a function of interest Interact with 14 genes of interest (AP2, CCL2, CCL5, LEP, etc…) -> 504 gene pairs ...
Supplementary figure legends
Supplementary figure legends

... YFP mRNA. The number of cells from whom the RNA was extracted was used for the normalization. Protein decay was estimated by monitoring CFP and YFP fluorescence by flow cytometry after different delays of treatment. Both mRNA and proteins decay following the respective treatments was fitted to an ex ...
Fig. 17.1 Levels at which gene expression can be controlled in
Fig. 17.1 Levels at which gene expression can be controlled in

... • What is alternative splicing? How is this used in the sexdetermination genes in Drosophila? • What are the Homeotic genes? – How are the Homeotic genes arranged on the chromosome and how ...
Leukaemia Section t(3;18)(q26;q11) Atlas of Genetics and Cytogenetics in Oncology and Haematology
Leukaemia Section t(3;18)(q26;q11) Atlas of Genetics and Cytogenetics in Oncology and Haematology

... likely to be cell-type dependant; antiapoptotic factor; involved in neuronal development organogenesis; role in hematopoietic differentiation ...
Increased Platform Concordance by Analyzing Gene Sets
Increased Platform Concordance by Analyzing Gene Sets

Tutorial_7 (2016) - Gene Expression
Tutorial_7 (2016) - Gene Expression

... The Gene Ontology project provides an ontology of defined terms representing gene product properties. ...
Gene Section YBX1 (Y box binding protein 1)
Gene Section YBX1 (Y box binding protein 1)

... This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 2.0 France Licence. © 2011 Atlas of Genetics and Cytogenetics in Oncology and Haematology ...
Tips for mining and integrating the Allen Mouse Brain Atlas data
Tips for mining and integrating the Allen Mouse Brain Atlas data

Ciliated dendrite mRNA may control olfactory sensory neuron
Ciliated dendrite mRNA may control olfactory sensory neuron

iCLIP HeLa cells were UV crosslinked before lysing in lysis buffer
iCLIP HeLa cells were UV crosslinked before lysing in lysis buffer

... overlapping genes, the shorter gene always has the priority). Introns, 5’ UTR, ORF and 3’ UTR were considered as separate regions. (ii) iCLIP reads antisense to the transcriptional direction of the associated gene, and reads that mapped to non-annotated genomic regions, were removed before ...
Chapter 14: Gene Expression: From Gene to Protein
Chapter 14: Gene Expression: From Gene to Protein

... Use Figure 14.9 in your text to label the following elements of the figure below: TATA box, RNA polymerase II, transcription factors, template DNA strand, start point, 5' and 3', and mRNA transcript. To the right of the figure, explain the three stages of initiation that are shown. ...
Neonatal diabetes: What can genetics teach us about the endocrine
Neonatal diabetes: What can genetics teach us about the endocrine

The ABC`s of DNA - High Point University
The ABC`s of DNA - High Point University

... but a few of sequences are still active and may do some good. The chromosomes themselves have a rich archaeology. Large blocks of genes seem to have been extensively copied from one human chromosome to another, beckoning genetic archaeologists to figure out the order in which the copying occurred an ...
All Alus are approximately 300 bp in length and derive
All Alus are approximately 300 bp in length and derive

... hundreds of thousands of Alu copies have accumulated in primates since their separation from other vertebrate groups about 65 million years ago. • Once an Alu inserts at a chromosome locus, it can copy itself for transposition, but there is no evidence that it is ever excised or lost from a chromoso ...
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Long non-coding RNA

Long non-coding RNAs (long ncRNAs, lncRNA) are non-protein coding transcripts longer than 200 nucleotides. This somewhat arbitrary limit distinguishes long ncRNAs from small regulatory RNAs such as microRNAs (miRNAs), short interfering RNAs (siRNAs), Piwi-interacting RNAs (piRNAs), small nucleolar RNAs (snoRNAs), and other short RNAs.
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