Parallel Genetic and Phenotypic Evolution of DNA Superhelicity in
... of these genetic changes were demonstrated, by constructing and competing otherwise isogenic strains, to be beneficial in the environment of the evolution experiment. Complete genome sequences were recently obtained for evolved clones isolated at several generations from one population. A total of 4 ...
... of these genetic changes were demonstrated, by constructing and competing otherwise isogenic strains, to be beneficial in the environment of the evolution experiment. Complete genome sequences were recently obtained for evolved clones isolated at several generations from one population. A total of 4 ...
Repair of Site-Specific DNA Double-Strand Breaks in
... double-hemizygous hybrids (DU.GUS/WT–I-SceI/WT, IU.GUS/ WT–I-SceI/WT, and GU.US/WT–I-SceI/WT) has been confirmed via quantitative RT-PCR (Supplemental Figure 1). Leaves of adult plants were exposed to GUS staining and revealed in GU.US/WT–I-SceI/WT hybrid plants two regions with the expected blue col ...
... double-hemizygous hybrids (DU.GUS/WT–I-SceI/WT, IU.GUS/ WT–I-SceI/WT, and GU.US/WT–I-SceI/WT) has been confirmed via quantitative RT-PCR (Supplemental Figure 1). Leaves of adult plants were exposed to GUS staining and revealed in GU.US/WT–I-SceI/WT hybrid plants two regions with the expected blue col ...
Modeling Fragile X Syndrome Using Human Pluripotent Stem Cells
... Despite the tight correlation between transcriptional inactivation and chromatin compaction, the precise mechanism governing the silencing of FMR1 is not fully understood. In addition, it remains unclear when FMR1 gene inactivation takes place during embryo development. Previous reports related to t ...
... Despite the tight correlation between transcriptional inactivation and chromatin compaction, the precise mechanism governing the silencing of FMR1 is not fully understood. In addition, it remains unclear when FMR1 gene inactivation takes place during embryo development. Previous reports related to t ...
Nanopore Unzipping of Individual DNA Hairpin Molecules
... under constant force or constant loading rate. Using a dynamic voltage control method, the entry rate of polynucleotides into the pore and the voltage pattern applied to induce hairpin unzipping are independently set. Thus, hundreds of unzipping events can be tested in a short period of time (few mi ...
... under constant force or constant loading rate. Using a dynamic voltage control method, the entry rate of polynucleotides into the pore and the voltage pattern applied to induce hairpin unzipping are independently set. Thus, hundreds of unzipping events can be tested in a short period of time (few mi ...
Genetics of Down Syndrome
... The time of the first conference for nomenclature in 1959 is called the pre-banding area. Individual chromosomes could not yet be ascertained beyond reasonable doubt. Thus it happened that the second smallest chromosome, chromosome 21, which had been analysed three times in the patient’s karyotype, ...
... The time of the first conference for nomenclature in 1959 is called the pre-banding area. Individual chromosomes could not yet be ascertained beyond reasonable doubt. Thus it happened that the second smallest chromosome, chromosome 21, which had been analysed three times in the patient’s karyotype, ...
In the near future, EU thresholds for adventitious
... the seed pool tests. This did not occur to a problematic level in this study because only lower numbers than expected of positive seed pools were observed. However, we also suspect that under-mixing of the 0.9% GM seed analytical sample had occurred. If more vigorous mixing had been used it is possi ...
... the seed pool tests. This did not occur to a problematic level in this study because only lower numbers than expected of positive seed pools were observed. However, we also suspect that under-mixing of the 0.9% GM seed analytical sample had occurred. If more vigorous mixing had been used it is possi ...
transposon
... x M female crosses, but not by M male x P female crosses. A P strain carries 30-50 copies of the P element, about a third of them full length. The elements are absent from M strains. In a P strain, the elements are carried as inert components of the genome. But they become activated to transpose w ...
... x M female crosses, but not by M male x P female crosses. A P strain carries 30-50 copies of the P element, about a third of them full length. The elements are absent from M strains. In a P strain, the elements are carried as inert components of the genome. But they become activated to transpose w ...
Classification of colorectal cancer based on correlation of clinical
... absent or very rarely detected in sporadic MSI-H CRC.26,31,32 The non-involvement of APC and CTNNB1 in sporadic MSI-H CRC is fully supported by the immunoexpression pattern for b-catenin, in which the normal distribution along lateral cell membranes is maintained while aberrant translocation to the ...
... absent or very rarely detected in sporadic MSI-H CRC.26,31,32 The non-involvement of APC and CTNNB1 in sporadic MSI-H CRC is fully supported by the immunoexpression pattern for b-catenin, in which the normal distribution along lateral cell membranes is maintained while aberrant translocation to the ...
GENtle, a free multi-purpose molecular biology tool
... information. This trend notwithstanding, general purpose software for these tasks often suffers from severe drawbacks. Free software exists, but is often hard to set up and operate for users on today’s point-and-click interfaces, and usually leads to the application of a patch-work of multiple, only ...
... information. This trend notwithstanding, general purpose software for these tasks often suffers from severe drawbacks. Free software exists, but is often hard to set up and operate for users on today’s point-and-click interfaces, and usually leads to the application of a patch-work of multiple, only ...
Figures and figure supplements
... Developmental Biology and Stem Cells Genomics and Evolutionary Biology ...
... Developmental Biology and Stem Cells Genomics and Evolutionary Biology ...
Identification and characterisation of Bacillus subtilis as cellulase
... same strain based on the 16S rDNA gene sequences. Amplification using two primer sets encoding CelL15 and CelL73 genes showed that only C12 contained both the cellulase genes while C4 and EB6 contained only one cellulase gene. Assays on their individual enzyme activities (total cellulase activity) w ...
... same strain based on the 16S rDNA gene sequences. Amplification using two primer sets encoding CelL15 and CelL73 genes showed that only C12 contained both the cellulase genes while C4 and EB6 contained only one cellulase gene. Assays on their individual enzyme activities (total cellulase activity) w ...
DNA METHODS FOR HLA TYPING A WORKBOOK FOR - ASHI-U
... in the DNA will be important for other methods of DNA typing. Also, next generation sequencing ligates DNA fragments together in library creation and this is a topic covered in this section. Restriction endonucleases (RE) are enzymes that cut double-stranded DNA at a specific sequence of base pairs. ...
... in the DNA will be important for other methods of DNA typing. Also, next generation sequencing ligates DNA fragments together in library creation and this is a topic covered in this section. Restriction endonucleases (RE) are enzymes that cut double-stranded DNA at a specific sequence of base pairs. ...
Unit 3 Resource
... Corn is a good organism for studying genetics because many phenotypes can be seen in an ear of corn. Also, corn plants are easy to work with, and crosses can easily be made. Because each kernel is a separate seed, a single ear of corn contains many offspring. The more offspring you can count from a ...
... Corn is a good organism for studying genetics because many phenotypes can be seen in an ear of corn. Also, corn plants are easy to work with, and crosses can easily be made. Because each kernel is a separate seed, a single ear of corn contains many offspring. The more offspring you can count from a ...
Engineered Cpf1 Enzymes with Altered PAM Specificities
... reducing the practical utility of Cpf1. To address this limitation, we aimed to engineer variants of Cpf1 that can recognize alternative PAM sequences in order to increase its targeting range. Previous work has shown that the PAM preference of Cas9 can be altered by mutations to residues in close pr ...
... reducing the practical utility of Cpf1. To address this limitation, we aimed to engineer variants of Cpf1 that can recognize alternative PAM sequences in order to increase its targeting range. Previous work has shown that the PAM preference of Cas9 can be altered by mutations to residues in close pr ...
Environmental Microbiology
... Colony phase variation is a regulatory mechanism at the DNA level which usually results in high frequency, reversible switches between colonies with a different phenotype. A number of molecular mechanisms underlying phase variation are known: slipped-strand mispairing, genomic rearrangements, sponta ...
... Colony phase variation is a regulatory mechanism at the DNA level which usually results in high frequency, reversible switches between colonies with a different phenotype. A number of molecular mechanisms underlying phase variation are known: slipped-strand mispairing, genomic rearrangements, sponta ...
CBSE XII BIO QS with key(2009-2015)
... they have food reserves for the seedling or embryo the seed coat provides protection to the embryo generate genetic variation remain viable for a considerable period of time.(any two) =1x2 State what is apomixis? Comment on its significance. How can it be commercially used? (2015) Form of asexual re ...
... they have food reserves for the seedling or embryo the seed coat provides protection to the embryo generate genetic variation remain viable for a considerable period of time.(any two) =1x2 State what is apomixis? Comment on its significance. How can it be commercially used? (2015) Form of asexual re ...
Sequence requirements for function of the
... gene loci (Asano and Wharton, 1999; Calvi et al., 1998; Royzman et al., 1999). The data demonstrate that Drosophila chorion gene amplification uses evolutionarily conserved machinery for initiation; however, some mechanism must exist to uniquely mark the chorion gene loci origins for activation duri ...
... gene loci (Asano and Wharton, 1999; Calvi et al., 1998; Royzman et al., 1999). The data demonstrate that Drosophila chorion gene amplification uses evolutionarily conserved machinery for initiation; however, some mechanism must exist to uniquely mark the chorion gene loci origins for activation duri ...
HL7 V2.5.1 Genetic Test Result Message
... genomic and healthcare IT data standards may use this guide to extend these standards for support of clinical sequencing. Users of this guide must be familiar with the details of HL7 message construction and processing. This guide is not intended to be a tutorial on that subject. ...
... genomic and healthcare IT data standards may use this guide to extend these standards for support of clinical sequencing. Users of this guide must be familiar with the details of HL7 message construction and processing. This guide is not intended to be a tutorial on that subject. ...
An assessment of factors affecting the likelihood
... Suggested transfers of genes from plants (or other eukaryotes) to bacteria are controversial, and they are estimated to have occurred several million years ago. Recent whole-bacterial genome sequencing has suggested that up to 16% of protein-encoding DNA in bacteria has been horizontally acquired, b ...
... Suggested transfers of genes from plants (or other eukaryotes) to bacteria are controversial, and they are estimated to have occurred several million years ago. Recent whole-bacterial genome sequencing has suggested that up to 16% of protein-encoding DNA in bacteria has been horizontally acquired, b ...
Prediction and investigation of novel proteins in DNA double
... broken ends to close proximity and Dnl4/Lif1/Nej1 ligates the DNA ends. Protein-protein interaction (PPI) has been utilized in functional genomics studies to identify novel proteins involved in different pathways based on their PPI profiles. In this study we aim to screen for novel proteins involved ...
... broken ends to close proximity and Dnl4/Lif1/Nej1 ligates the DNA ends. Protein-protein interaction (PPI) has been utilized in functional genomics studies to identify novel proteins involved in different pathways based on their PPI profiles. In this study we aim to screen for novel proteins involved ...
Physical Mapping of a 670-kb Region of Chromosomes XVI and XVII
... To test whether the YAC clones harboring genes h49 and jl8 are mitotically stable in long term cultures, they were grown for 75 generations and examined by Southern blot analysis. No rearrangements were observed suggesting the YAC clones are propagated faithfully during mitosis. This observation was ...
... To test whether the YAC clones harboring genes h49 and jl8 are mitotically stable in long term cultures, they were grown for 75 generations and examined by Southern blot analysis. No rearrangements were observed suggesting the YAC clones are propagated faithfully during mitosis. This observation was ...
A fost luat în studiu caracterul multifoliolar deoarece acest caracter
... trait was not observed in any of the descendants. Further on it was emphasized for 14% of the descendants: the number of leaflets per each leave varied between 4 and 6. From the F2 individuals, which were divided into two categories depending on the presence or absence of multileaflet trait, the B1 ...
... trait was not observed in any of the descendants. Further on it was emphasized for 14% of the descendants: the number of leaflets per each leave varied between 4 and 6. From the F2 individuals, which were divided into two categories depending on the presence or absence of multileaflet trait, the B1 ...
The full-length HIV-1 molecular clone pLAI 61 was used to produce
... capped G residue. Supplementary Table 1 lists all oligonucleotides used in this study. The tat exon 1 was amplified by PCR on pLAI with primers NotI-WdV005 and WdV002; tat exon 2 with primers WdV007 and NotI-WdV004; rev exon 1 with primers NotI-WdV001 and WdV002; rev exon 2 with primers WdV003 and N ...
... capped G residue. Supplementary Table 1 lists all oligonucleotides used in this study. The tat exon 1 was amplified by PCR on pLAI with primers NotI-WdV005 and WdV002; tat exon 2 with primers WdV007 and NotI-WdV004; rev exon 1 with primers NotI-WdV001 and WdV002; rev exon 2 with primers WdV003 and N ...
Epigenetics & Chromatin Xist through transcriptional control of Dnmt3a
... establishment of X inactivation in early embryogenesis the inactive state is stably maintained through all subsequent cell generations (reviewed in [1]). X inactivation is triggered by the expression of the X inactive specific transcript (Xist), an unusual non-coding RNA that has the unique property ...
... establishment of X inactivation in early embryogenesis the inactive state is stably maintained through all subsequent cell generations (reviewed in [1]). X inactivation is triggered by the expression of the X inactive specific transcript (Xist), an unusual non-coding RNA that has the unique property ...
Am I a Warrior? - Erasmus University Thesis Repository
... Cortisol is a steroid hormone which is produced in the zona fasciculate of the adrenal cortex as a response to any form of stress. Both physical stress and psychological stress causes the release of this hormone. This is why Cortisol is also called the stress hormone. Physical and psychological stre ...
... Cortisol is a steroid hormone which is produced in the zona fasciculate of the adrenal cortex as a response to any form of stress. Both physical stress and psychological stress causes the release of this hormone. This is why Cortisol is also called the stress hormone. Physical and psychological stre ...