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Physical map of the aromatic amine and m-toluate
Physical map of the aromatic amine and m-toluate

... approaches ensures its integrity : (a) single and double digestion of the native plasmid; (6) cloning of fragments HB, HC, HD, HE, XC, XD, XE and EC, determination of their restriction profiles, and hybridization to variously digested pTDN 1 ; (c) restriction digestion of a variety of deletion mutan ...
Conspiracy of silence among repeated transgenes
Conspiracy of silence among repeated transgenes

... conjecture might be correct. When vertebrates are transformed with exogenous DNA, chromosomal insertions almost always consist of long tandem arrays of the transfected transgene-containing DNA.(5) Sometimes transgene arrays can comprise hundreds or even thousands of copies. In a cytologic study of o ...
DNA Evolution 3.1 Troubleshooting and Debugging Guide
DNA Evolution 3.1 Troubleshooting and Debugging Guide

... © 2013 StorageDNA, Inc. All rights reserved. The owner or authorized user of a valid copy of DNA Evolution may reproduce this publication for the purpose of learning to use such software. No part of this publication may be reproduced or transmitted for commercial purposes, such as ...
Mycobacterium kyorinense sp. nov., a novel, slow
Mycobacterium kyorinense sp. nov., a novel, slow

... the Mycobacterium avium complex, and is also seen in M. celatum and M. branderi, suggesting that the new isolates are closely related to these Mycobacterium species (Fig. 1) (Butler et al., 1993; Brander et al., 1992). The sequence of the 16S rRNA gene was identical in all strains, but was different ...
Structural organization of the transfer RNA gene clusters of cholera
Structural organization of the transfer RNA gene clusters of cholera

... most cases, host aminoacyl-tRNA synthetases and tRNAs. However, infections of Escherichia coli cells with T5 (Scherberg and Weiss 1970) and T-even phages (McClain et al 1972; Wilson et al 1972; Desai and Weiss 1977) induce the synthesis of a large number of tRNA species coded by the phage genome. A ...
Q 4 - Good Laboratory Practice when Performing Molecular
Q 4 - Good Laboratory Practice when Performing Molecular

... describes procedures that will help to minimise the carry-over of amplified DNA. Whilst the guidelines concern the majority of PCR applications, they are most relevant where ‘in-house’ assays are in use and may be less relevant when using commercial kits, and to other amplification procedures. These ...
Aus dem  Max-Planck-Institut für Psychiatrie
Aus dem Max-Planck-Institut für Psychiatrie

... 1.2 Genetic Mouse Models in Psychiatric Research The existence of a genetic component in the pathogenesis and maintenance of psychiatric disorders has long been recognized from twin and adoption studies. Nowadays, genetic linkage analysis even allows the precise identification of genetic loci involv ...
Transcription
Transcription

... The role of s factor in promoter binding • The RNA polymerase core enyzme, a2bb’w, has a general non-specific affinity for DNA, which is referred to as loose binding that is fairly stable. • The addition of s factor to the core enzyme markedly reduces the holoenzyme affinity for non-specific bindin ...
Multiplex in-vitro Detection using SERS
Multiplex in-vitro Detection using SERS

... experimental conditions for FAM- and TAMRA-labelled DNA.78 They found that dyespermine and dye-DNA interactions cause changes in the observed fluorescence intensity, which is also dependant on experimental conditions such as the pH and concentration. It was also observed that these fluctuations in i ...
Identification of Genetic and Epigenetic Risk Factors for Psoriasis
Identification of Genetic and Epigenetic Risk Factors for Psoriasis

... indicating that other genetic effects and environmental triggers are important. In order to identify novel susceptibility genes of PS and PsA, I performed the first large scale genome wide association scan for psoriasis susceptibility loci using 233 cases and 519 controls. It revealed that genes of ...
Chromosomal breakpoint positions suggest a direct role for radiation
Chromosomal breakpoint positions suggest a direct role for radiation

... (Santoro et al., 1993). However, malignant phenotype was obtained by cotransfection with H-RAS or K-RAS oncogenes. An alternative to the direct pathway is that radiation does not act directly to produce the RET/PTC3 fusion, but instead may cause persistent genomic instability, which eventually produ ...
lntraclonal mating in Trypanosoma brucei is
lntraclonal mating in Trypanosoma brucei is

... kinetoplast DNA (kDNA), which is the mitochondrial DNA of trypanosomes, has shown that one DNA component (maxicircles) is inherited uniparentally, while the other (minicircles) is inherited from both parents (Gibson & Garside, 1990). The generation of hybrid kDNA networks implies that both cellular ...
Gene Detection Systems Catalog
Gene Detection Systems Catalog

... your name from the dropdown USER name field. If you are a 'new' user, select NEW and enter your name, email address and telephone number. This information will be stored and your name will appear the next time you login. Order Changes and Cancellation All catalog product orders are routinely shipped ...
bacterial plasmids - Acta Medica Medianae
bacterial plasmids - Acta Medica Medianae

... transfer, or oriT. This spot is asymmetric and it is oriented in such a way so genetic region, which controls the transfer, transfers at the end. OriT sequences differ among plasmids and they are very specific. For mobilization of non-conjugative plasmids, an interaction between theirs oriT sequence ...
Investigating the DNA-Binding Interactions of Small
Investigating the DNA-Binding Interactions of Small

... and other disorders. In this dissertation, the DNA-binding interactions of small organic molecules were investigated. We developed a highly sensitive methodology to determine the DNA-binding mode of small molecules unambiguously unlike other conventional techniques. Furthermore, we established struc ...
Press release
Press release

... extent on the sample material itself (leaf and seed material, animal tissue, hair, etc.). sbeadex - high-quality DNA preparations The oKtopure utilises the sbeadexTM extraction chemistry developed by LGC, which is based on the use of magnetic particles. These particles can specifically bind nucleic ...
Characterizing a Lambda Red Recombinase Induced Presumptive
Characterizing a Lambda Red Recombinase Induced Presumptive

... type E.coli C29 were grown overnight in LB broth plus 10% glycerol and LB broth plus 10% glycerol and 1 mM isopropylthiogalactoside (IPTG). Cultures were incubated overnight at 37ºC with aeration. After incubation, 2 mL of each culture was removed from culture tube and the OD660 was measured. 200 µL ...
Erp, an extracellular protein family specific to
Erp, an extracellular protein family specific to

... plasmids were sequenced. The DNA sequences were assembled and the deduced amino acid sequences were shown to contain repeated sequences based on the PGLTS motif, as already described for the M. tuberculosis and M. leprae proteins. Fig. 1 shows a schematic representation of a multiple alignment of al ...
microbial genetics
microbial genetics

... transfer occurs through transformation and conjugation. Often it is useful to transfer a nontransmissible plasmid to a specific host cell. It is possible to transfer the purified DNA as long as a genetic selection is available for recipients that possess the plasmid. Uptake of purified DNA is called ...
Gene Section ID4 (inhibitor of DNA binding 4, dominant negative helix-loop-helix protein)
Gene Section ID4 (inhibitor of DNA binding 4, dominant negative helix-loop-helix protein)

... significant risk factor for nodal metastasis (Umetani et al., 2006). In another study, BRCA1, ER (estrogen receptor), and ID4 were found expressed in breast cancer specimens from patients with invasive carcinomas. Most of the patients who expressed BRCA1 also expressed ER, but were negative for ID4, ...
Imprinted gene expression in hybrids: perturbed
Imprinted gene expression in hybrids: perturbed

... The developmental establishment and maintenance of imprinting involve complex interactions between cis-regulatory elements and trans-acting factors, some of which are themselves subject to imprinting (Kelsey and Feil, 2013; Wolf, 2013). The transcription factor gene ZAC1 is imprinted in both mice an ...
light - Microbiology
light - Microbiology

... fragments, also induces new donor bacteria. Other agents such as X-rays and methyl methansulphonate (MMS) do not stimulate the production of new donors but may enhance the recombination frequency since cells killed by them may continue to act as chromosome donors. The effect is not shown either by u ...
Ultraviolet Induction of Chromosome Transfer by
Ultraviolet Induction of Chromosome Transfer by

... fragments, also induces new donor bacteria. Other agents such as X-rays and methyl methansulphonate (MMS) do not stimulate the production of new donors but may enhance the recombination frequency since cells killed by them may continue to act as chromosome donors. The effect is not shown either by u ...
Identifikasi Molekular Bakteri Pathogen yang Menginfeksi Hama
Identifikasi Molekular Bakteri Pathogen yang Menginfeksi Hama

... also exist within bacterial entomopathogen species and may have different pathogenic or virulence levels to the insect host. Therefore, rapid and accurate detection of bacteria is needed to differentiate them from others according to their role and to potentially use them as biological control. It h ...
Standard
Standard

... (Medicago) as it has a number of attractive advantages. Flowering in Medicago is regulated by vernalisation and long day length, it has diploid in-breeding genetics, a moderate-sized sequenced genome of about 550 Mb and large mutant populations (Clarkson and Russell, 1975; Benlloch et al., 2006; Ros ...
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Epigenomics

Epigenomics is the study of the complete set of epigenetic modifications on the genetic material of a cell, known as the epigenome. The field is analogous to genomics and proteomics, which are the study of the genome and proteome of a cell (Russell 2010 p. 217 & 230). Epigenetic modifications are reversible modifications on a cell’s DNA or histones that affect gene expression without altering the DNA sequence (Russell 2010 p. 475). Two of the most characterized epigenetic modifications are DNA methylation and histone modification. Epigenetic modifications play an important role in gene expression and regulation, and are involved in numerous cellular processes such as in differentiation/development and tumorigenesis (Russell 2010 p. 597). The study of epigenetics on a global level has been made possible only recently through the adaptation of genomic high-throughput assays (Laird 2010) and.
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