Molecular Diagnostics in Clinical Microbiology
... In the last two decades, strategies based on nucleic acid amplification techniques (NAATs) have taken an irreversible position in the diagnostic field of infectious diseases. Pathogens can be detected in qualitative and quantitative NAAT strategies by selection of species-specific nucleic acid targe ...
... In the last two decades, strategies based on nucleic acid amplification techniques (NAATs) have taken an irreversible position in the diagnostic field of infectious diseases. Pathogens can be detected in qualitative and quantitative NAAT strategies by selection of species-specific nucleic acid targe ...
A Novel PCR Detection Method for Major Fish Pathogenic Bacteria
... API 20E system (Grisezet al., 1991), fluorescent antibody technique (Miyamoto and Eguchi, 1997), pulsed-field gel electrophoresis analysis (Skov et al., 1995), and DNA hybridization (Martinez-Picadoet al., 1996). However, these methods require considerable time and effort to identify V. anguillarum. ...
... API 20E system (Grisezet al., 1991), fluorescent antibody technique (Miyamoto and Eguchi, 1997), pulsed-field gel electrophoresis analysis (Skov et al., 1995), and DNA hybridization (Martinez-Picadoet al., 1996). However, these methods require considerable time and effort to identify V. anguillarum. ...
Structural and Functional Studies of Insertion Element IS200
... and are described in this paper. (i) Construction of strains containing hisG : : TnlO and nonpolar deletion derizdives of hisD984 : : IS200 Non-polar deletion derivatives of hisD984 : : IS200 have restored transcription of the adjacent downstream hid gene by removing the transcription stop signal at ...
... and are described in this paper. (i) Construction of strains containing hisG : : TnlO and nonpolar deletion derizdives of hisD984 : : IS200 Non-polar deletion derivatives of hisD984 : : IS200 have restored transcription of the adjacent downstream hid gene by removing the transcription stop signal at ...
Article Mitochondrial DNA turnover occurs during preimplantation
... by a mitochondrial specific DNA polymerase γ (Lestienne, 1987). The exact molecular mechanism controlling the amount of mtDNA per cell is still uncertain (Moraes, 2001; Bogenhagen and Clayton, 2003). Concentrations of mtDNA are very sensitive to environmental stress, and a sub-optimal environment ca ...
... by a mitochondrial specific DNA polymerase γ (Lestienne, 1987). The exact molecular mechanism controlling the amount of mtDNA per cell is still uncertain (Moraes, 2001; Bogenhagen and Clayton, 2003). Concentrations of mtDNA are very sensitive to environmental stress, and a sub-optimal environment ca ...
Maintenance of genomic integrity by p53: complementary
... Haupt et al., 1995; reviewed in Bates and Vousden, 1999). The interaction of p53 with CAK already described above could be one such mechanism, but other interactions, mediated by the N-terminal prolinerich domain of p53 which serves as a docking place for proteins containing SH3-domains, have also b ...
... Haupt et al., 1995; reviewed in Bates and Vousden, 1999). The interaction of p53 with CAK already described above could be one such mechanism, but other interactions, mediated by the N-terminal prolinerich domain of p53 which serves as a docking place for proteins containing SH3-domains, have also b ...
No Slide Title
... • Example in M: phosphorylation of nuclear lamins by cyclin B/cdk1 results in disassembly of the nuclear lamina, a fibrous layer that forms the wall of the nucleus • Example in G1: phosphorylation of Rb (retinoblastoma protein) by cyclin D/cdk4 causes it to release the transcriptional factor E2F. Th ...
... • Example in M: phosphorylation of nuclear lamins by cyclin B/cdk1 results in disassembly of the nuclear lamina, a fibrous layer that forms the wall of the nucleus • Example in G1: phosphorylation of Rb (retinoblastoma protein) by cyclin D/cdk4 causes it to release the transcriptional factor E2F. Th ...
mMESSAGE mMACHINE® Kit User Guide
... mMESSAGE mMACHINE® Kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5' end. mMESSAGE mMACHINE® Kit reactions include cap analog [m7G(5')ppp(5')G] in an ultra hi ...
... mMESSAGE mMACHINE® Kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5' end. mMESSAGE mMACHINE® Kit reactions include cap analog [m7G(5')ppp(5')G] in an ultra hi ...
Structure and function of the GINS complex, a key component of the
... of GINS in development and disease avoidance [15–19]. Finally, recent results suggest that the GINS function might be regulated in response to DNA damage [20]. ...
... of GINS in development and disease avoidance [15–19]. Finally, recent results suggest that the GINS function might be regulated in response to DNA damage [20]. ...
Analysis of DNA transcription termination sequences of gene coding
... the size of the intergenic region is the same (141 bp), and they are shorter in comparison with Pseudomonas sp. KBOS 04 (163 bp). More importantly, five of the analysed strains have a palindromic sequence, which is constituted of 24 bp with high GC content (Figure 2). The detailed analysis have indi ...
... the size of the intergenic region is the same (141 bp), and they are shorter in comparison with Pseudomonas sp. KBOS 04 (163 bp). More importantly, five of the analysed strains have a palindromic sequence, which is constituted of 24 bp with high GC content (Figure 2). The detailed analysis have indi ...
Analysis of Cross Sequence Similarities for Multiple - PolyU
... regions found within one chromosome sequence. Biocompress proposed by Grumbach and Tahi (1993) is the first algorithm designed specifically for compressing DNA sequences. Both Biocompress and its second version Biocompress-2 (Grumbach and Tahi, 1994) are based on a sliding window algorithm known as ...
... regions found within one chromosome sequence. Biocompress proposed by Grumbach and Tahi (1993) is the first algorithm designed specifically for compressing DNA sequences. Both Biocompress and its second version Biocompress-2 (Grumbach and Tahi, 1994) are based on a sliding window algorithm known as ...
7nQ Jj I f NO "7^07 - UNT Digital Library
... to understand. It offers a detailed look at all the components of genetics. It contains study questions as well as summaries for each chapter to provide an even better way to retain the knowledge from my lectures. The course that I have outlined is intended for a junior or senior college student. So ...
... to understand. It offers a detailed look at all the components of genetics. It contains study questions as well as summaries for each chapter to provide an even better way to retain the knowledge from my lectures. The course that I have outlined is intended for a junior or senior college student. So ...
Homologous recombination in budding yeast expressing the human
... expression was controlled by the ADH1 promoter and terminator sequences. Western blots of whole cell extracts from strains containing the adh1::HsRAD52-FLAG allele displayed a 49 kDa peptide consistent with that expected for HsRAD52-FLAG while extracts from strains containing the RAD52-FLAG allele y ...
... expression was controlled by the ADH1 promoter and terminator sequences. Western blots of whole cell extracts from strains containing the adh1::HsRAD52-FLAG allele displayed a 49 kDa peptide consistent with that expected for HsRAD52-FLAG while extracts from strains containing the RAD52-FLAG allele y ...
Replication timing as an epigenetic mark
... directly influenced by nor do they have a direct influence on transcription but rather define a level of higher-order organization of the genome,9,10,12 which is thought to affect transcriptional competence independent of transcription per se. Replication timing is therefore more in line with the co ...
... directly influenced by nor do they have a direct influence on transcription but rather define a level of higher-order organization of the genome,9,10,12 which is thought to affect transcriptional competence independent of transcription per se. Replication timing is therefore more in line with the co ...
Practice exam 3 key
... b) EcoRI cuts both strands of DNA. The position of the first cut is indicated by the arrow above. Draw an arrow to indicate the position of the second cut. (1 pt) See 1a. c) The position of the two cuts makes EcoRI a particularly useful tool for manipulating DNA. Explain. (3 pts) Leaves single-stran ...
... b) EcoRI cuts both strands of DNA. The position of the first cut is indicated by the arrow above. Draw an arrow to indicate the position of the second cut. (1 pt) See 1a. c) The position of the two cuts makes EcoRI a particularly useful tool for manipulating DNA. Explain. (3 pts) Leaves single-stran ...
Characterisation of marsupial PHLDA2 reveals eutherian specific acquisition of imprinting Open Access
... imprinted [11,17,26-33]. These are IGF2, IGF2R, PEG1/ MEST, PEG10, INS and H19, and are from 4 independent domains. We have previously reported that there is no evidence of genomic imprinting of CDKN1C (also known as p57KIP2) in a marsupial, the tammar wallaby (Macropus eugenii) [28,34]. CDKN1C is l ...
... imprinted [11,17,26-33]. These are IGF2, IGF2R, PEG1/ MEST, PEG10, INS and H19, and are from 4 independent domains. We have previously reported that there is no evidence of genomic imprinting of CDKN1C (also known as p57KIP2) in a marsupial, the tammar wallaby (Macropus eugenii) [28,34]. CDKN1C is l ...
Manual: FullVelocity(TM) SYBR® Green QPCR - Gene X
... results can be displayed as an amplification plot (Figure 1), which reflects the change in fluorescence during cycling. This information can be used to quantitate initial copy number based on threshold cycle (Ct).2 Ct is defined as the cycle at which fluorescence is statistically significant above b ...
... results can be displayed as an amplification plot (Figure 1), which reflects the change in fluorescence during cycling. This information can be used to quantitate initial copy number based on threshold cycle (Ct).2 Ct is defined as the cycle at which fluorescence is statistically significant above b ...
Gene Section USF1 (upstream transcription factor 1) Atlas of Genetics and Cytogenetics
... and A2 domains are important for E-box and initiator element (Inr)-dependent transactivation (Roy et al., 1997). Post-translational modifications that affect USF1 function are indicated. The protein product of the splice variant lacks the first 59 amino acids, dimerizes with full-length USF1 protein ...
... and A2 domains are important for E-box and initiator element (Inr)-dependent transactivation (Roy et al., 1997). Post-translational modifications that affect USF1 function are indicated. The protein product of the splice variant lacks the first 59 amino acids, dimerizes with full-length USF1 protein ...
Cloning and expression of the phosphotriesterase
... enzymic activity in library clones. In a previous study, an OP-degrading gene was successfully isolated from an Agrobacterium strain by mobilizing a genomic library into a related strain lacking activity (Horne et al., 2002a). A similar approach was taken here, using Pseudomonas aeruginosa BENNY, a ...
... enzymic activity in library clones. In a previous study, an OP-degrading gene was successfully isolated from an Agrobacterium strain by mobilizing a genomic library into a related strain lacking activity (Horne et al., 2002a). A similar approach was taken here, using Pseudomonas aeruginosa BENNY, a ...
Role of the p21 Protein in the Fanconi Anemia Pathway
... suppressor transcription factor p53 Figure 8.4 The Biology of Cancer (© Garland Science 2007) ...
... suppressor transcription factor p53 Figure 8.4 The Biology of Cancer (© Garland Science 2007) ...
The Use of Cytochrome B Sequence Variation in Estimation of
... Wilson (1990), Johnsonand Cicero (199 l), Richman and Price (1992) and Lanyon (1992) mitochondrial variation was characterized through restriction fragment analysis. In the latter papers, cytochrome b sequence variation was used to estimate genetic variation. Cytochrome b is essential for the proper ...
... Wilson (1990), Johnsonand Cicero (199 l), Richman and Price (1992) and Lanyon (1992) mitochondrial variation was characterized through restriction fragment analysis. In the latter papers, cytochrome b sequence variation was used to estimate genetic variation. Cytochrome b is essential for the proper ...
Capstone project 2015 in pdf format
... 1: Expression of valuable proteins in Escherichia coli 2: Expression of valuable proteins in Bacillus subtilis 3: Demystifying the mechanisms for intein-extein cleavages 1: Regulation of tumorigenesis by G protein signaling 2: Structure and function of Nm23 metastasis suppressors 1:The role of MST3 ...
... 1: Expression of valuable proteins in Escherichia coli 2: Expression of valuable proteins in Bacillus subtilis 3: Demystifying the mechanisms for intein-extein cleavages 1: Regulation of tumorigenesis by G protein signaling 2: Structure and function of Nm23 metastasis suppressors 1:The role of MST3 ...
Myriad--Ambry -- Final Version of Ambry Preliminary Injunction
... on the other strand in a very specific manner. In particular, adenine (A) can bind only to thymine (T) and guanine (G) can bind only to cytosine (C), and vice versa, and thus the nucleotides on each strand “complement” each other in this manner. A human gene is a section of DNA that contains a set o ...
... on the other strand in a very specific manner. In particular, adenine (A) can bind only to thymine (T) and guanine (G) can bind only to cytosine (C), and vice versa, and thus the nucleotides on each strand “complement” each other in this manner. A human gene is a section of DNA that contains a set o ...
NUCLEIC ACID ECONOMY IN BACTERIA INFECTED WITH
... survival of 10-~) in chilled centrifuge tubes. After about 30 minutes in an ice bath, the tubes were warmed 30 minutes at 37°C. and refrigerated overnight. The next morning I0 #g. desoxyribonuclease was added to each tube and the contents were diluted to 10 ml. with 0.1 per cent peptone water. After ...
... survival of 10-~) in chilled centrifuge tubes. After about 30 minutes in an ice bath, the tubes were warmed 30 minutes at 37°C. and refrigerated overnight. The next morning I0 #g. desoxyribonuclease was added to each tube and the contents were diluted to 10 ml. with 0.1 per cent peptone water. After ...
DNA
... • Along with eye color and sex, other genes, such as body color and wing size, had a higher probability of being coinherited by the offspring Æ genes are linked. • Morgan hypothesized that the closer the genes were located on the a chromosome, the more often the genes ...
... • Along with eye color and sex, other genes, such as body color and wing size, had a higher probability of being coinherited by the offspring Æ genes are linked. • Morgan hypothesized that the closer the genes were located on the a chromosome, the more often the genes ...
(base) sequence of the genome might reflect biological information
... the biological information of DNA (base sequence) should be transferred to protein via mRNA (base sequence). That is, the information of the base sequence of DNA was transformed to the amino acid sequence by tRNAs corresponding to the base sequences of the mRNA on the ribosome [22]. However, the cod ...
... the biological information of DNA (base sequence) should be transferred to protein via mRNA (base sequence). That is, the information of the base sequence of DNA was transformed to the amino acid sequence by tRNAs corresponding to the base sequences of the mRNA on the ribosome [22]. However, the cod ...