Figures 1
... of a 5–6 Mb region from the paternally contributed chromosome 15 (found in 65–75% of affected individuals); (ii) maternal uniparental disomy (UPD) 15 (found in 20–30%); and (iii) a defect in the genomic region that controls the imprinting process, a so-called imprinting defect (ID; 1–3%). IDs are us ...
... of a 5–6 Mb region from the paternally contributed chromosome 15 (found in 65–75% of affected individuals); (ii) maternal uniparental disomy (UPD) 15 (found in 20–30%); and (iii) a defect in the genomic region that controls the imprinting process, a so-called imprinting defect (ID; 1–3%). IDs are us ...
Biology II Final Exam Practice
... 87. A ________________ mutation causes all bases after the mutation to be out of order. 88. Sex-linked traits are all found on the ______________ chromosome. 89. The process by which an individual is exactly copied is called _____________ 90. The snake pelvis is an example of a ____________________ ...
... 87. A ________________ mutation causes all bases after the mutation to be out of order. 88. Sex-linked traits are all found on the ______________ chromosome. 89. The process by which an individual is exactly copied is called _____________ 90. The snake pelvis is an example of a ____________________ ...
DNA supercoiling factor contributes to dosage
... chromosome (Deuring et al., 2000; Corona et al., 2002; Badenhorst et al., 2002). The chromosome defect in the mutant strains is likely to be the result of an imbalance caused by acetylation by MOF leading to a more open chromatin state, without ISWI working toward a more condensed chromatin state (D ...
... chromosome (Deuring et al., 2000; Corona et al., 2002; Badenhorst et al., 2002). The chromosome defect in the mutant strains is likely to be the result of an imbalance caused by acetylation by MOF leading to a more open chromatin state, without ISWI working toward a more condensed chromatin state (D ...
1 - IPPC
... indicated in Figures 1 and 2 are the minimum requirements for the diagnosis, but further tests may be required by the national plant protection organization (NPPO), especially for the first report in a country. For example, serological tests may facilitate a presumptive diagnosis of symptomatic plan ...
... indicated in Figures 1 and 2 are the minimum requirements for the diagnosis, but further tests may be required by the national plant protection organization (NPPO), especially for the first report in a country. For example, serological tests may facilitate a presumptive diagnosis of symptomatic plan ...
Single-stranded heteroduplex intermediates in l Red homologous
... Two alternatives to the standard model were discussed by Paques and Haber based on observations made in S. cerevisiae [15]. They favoured the evidence of Morrow et al [17] for a double primed replication event that has similarities to break induced replication (BIR), leading to extensive chromosomal ...
... Two alternatives to the standard model were discussed by Paques and Haber based on observations made in S. cerevisiae [15]. They favoured the evidence of Morrow et al [17] for a double primed replication event that has similarities to break induced replication (BIR), leading to extensive chromosomal ...
A survey on computational methods for enhancer and
... conformation capture (Hi-C) (Lieberman-Aiden et al. 2009) and chromatin interaction analysis with paired-end tag sequencing (ChIA-PET) (Fullwood et al. 2009). Hi-C and ChIA-PET have facilitated whole-genome identification of DNA regions that are in close proximity in the three-dimensional genome str ...
... conformation capture (Hi-C) (Lieberman-Aiden et al. 2009) and chromatin interaction analysis with paired-end tag sequencing (ChIA-PET) (Fullwood et al. 2009). Hi-C and ChIA-PET have facilitated whole-genome identification of DNA regions that are in close proximity in the three-dimensional genome str ...
`Candidatus Phytoplasma mali`, `Candidatus Phytoplasma pyri` and
... group, 2004). According to these recommendations, ‘a phytoplasma can be described as a novel ‘Candidatus Phytoplasma’ species if its 16S rDNA sequence has <97?5 % similarity to that of any previously described ‘Candidatus Phytoplasma’ species’. In cases where phytoplasmas share ¢97?5 % 16S rDNA sequ ...
... group, 2004). According to these recommendations, ‘a phytoplasma can be described as a novel ‘Candidatus Phytoplasma’ species if its 16S rDNA sequence has <97?5 % similarity to that of any previously described ‘Candidatus Phytoplasma’ species’. In cases where phytoplasmas share ¢97?5 % 16S rDNA sequ ...
The Nicotiana tabacum genome encodes two cytoplasmic
... The Arabidopsis histone H4 clone, A748, was provided by C. Gigot (Chaboute et al. 1987). A748 contains a 196 bp fragment in the H4 coding region, which was excised and labelled using the same Multiprime technique. Southern blot and gene copy-number reconstruction analysis. DNA was extracted from dif ...
... The Arabidopsis histone H4 clone, A748, was provided by C. Gigot (Chaboute et al. 1987). A748 contains a 196 bp fragment in the H4 coding region, which was excised and labelled using the same Multiprime technique. Southern blot and gene copy-number reconstruction analysis. DNA was extracted from dif ...
to view
... is very small. Why such type of adjustment is there in higher organisms? Ans.Female gamete is large and non motile, is an adaptation for storing more food which will be required for the future development. The male gamete has to move to reach the counterpart, so it has the machinery for its reaching ...
... is very small. Why such type of adjustment is there in higher organisms? Ans.Female gamete is large and non motile, is an adaptation for storing more food which will be required for the future development. The male gamete has to move to reach the counterpart, so it has the machinery for its reaching ...
Regulatory Motifs in DNA Sequences
... • Nucleotides in motifs encode for a message in the “genetic” language. Symbols in “The Gold Bug” encode for a message in English • In order to solve the problem, we analyze the frequencies of patterns in DNA/Gold Bug ...
... • Nucleotides in motifs encode for a message in the “genetic” language. Symbols in “The Gold Bug” encode for a message in English • In order to solve the problem, we analyze the frequencies of patterns in DNA/Gold Bug ...
Notes for Chaper 4 of the Jones/Pevzer book
... • Nucleotides in motifs encode for a message in the “genetic” language. Symbols in “The Gold Bug” encode for a message in English • In order to solve the problem, we analyze the frequencies of patterns in DNA/Gold Bug message. • Knowledge of established regulatory motifs makes the Motif Finding prob ...
... • Nucleotides in motifs encode for a message in the “genetic” language. Symbols in “The Gold Bug” encode for a message in English • In order to solve the problem, we analyze the frequencies of patterns in DNA/Gold Bug message. • Knowledge of established regulatory motifs makes the Motif Finding prob ...
Warren, ST and Ashley, CT: Triplet repeat expansion mutations: The example of fragile X syndrome. Annual Review of Neuroscience 18:77-99 (1995).
... proximal and distal fragile X hybrid breakpoints and also detected length variation in fragile X patients on Southern blots. Kremer et al ( 199 1a) further localized this instability to a single 1.0-kb Pst I fragment, pfxa2, that was sequenced and found to contain an unusual CGG trinucleotide repeat ...
... proximal and distal fragile X hybrid breakpoints and also detected length variation in fragile X patients on Southern blots. Kremer et al ( 199 1a) further localized this instability to a single 1.0-kb Pst I fragment, pfxa2, that was sequenced and found to contain an unusual CGG trinucleotide repeat ...
OLSON LAB PROTOCOL: Agarose Gel Electrophoresis using GelRed
... • Gel electrophoresis is a method used to size fractionate, visualise and document DNA samples, such as those resulting from PCR • As DNA molecules are negatively charged, a current can be applied to a gel to draw the fragments toward the cathode (positive pole) • Larger fragments move more slowly t ...
... • Gel electrophoresis is a method used to size fractionate, visualise and document DNA samples, such as those resulting from PCR • As DNA molecules are negatively charged, a current can be applied to a gel to draw the fragments toward the cathode (positive pole) • Larger fragments move more slowly t ...
Specific PCR Primers for the Identification of Salmonella enterica
... Salmonella is one of the most common pathogens and a major cause of foodborne diseases in human worldwide (1,2,3). Contaminated poultry products have been identified as the principal sources of Salmonella leading to foodborne illness in human(4.5). The most common serotypes of Salmonella isolated fr ...
... Salmonella is one of the most common pathogens and a major cause of foodborne diseases in human worldwide (1,2,3). Contaminated poultry products have been identified as the principal sources of Salmonella leading to foodborne illness in human(4.5). The most common serotypes of Salmonella isolated fr ...
Simple identification of dominant p53 mutants by
... transactivate its target genes. While this abortive activation/ impaired degradation model provides a satisfactory explanation for the high level p53 expression seen in tumours (5,6), it does not rule out the possibility that a selective growth advantage may be conferred by mutated p53 proteins, eit ...
... transactivate its target genes. While this abortive activation/ impaired degradation model provides a satisfactory explanation for the high level p53 expression seen in tumours (5,6), it does not rule out the possibility that a selective growth advantage may be conferred by mutated p53 proteins, eit ...
PCR Clean-up Kit / 96-well PCR Clean
... cosmid DNA (per well) from up to 1.3 ml of bacterial culture. This method is based on a modified alkaline lysis of the bacteria in combination with the selective binding of the plasmid DNA to a special silica membrane. The purified plasmid or cosmid DNA is free from protein, genomic DNA and RNA and ...
... cosmid DNA (per well) from up to 1.3 ml of bacterial culture. This method is based on a modified alkaline lysis of the bacteria in combination with the selective binding of the plasmid DNA to a special silica membrane. The purified plasmid or cosmid DNA is free from protein, genomic DNA and RNA and ...
Identification and isolation of active N2O reducers in rice paddy soil
... phylogeny is generally in agreement with 16S rRNA gene phylogeny, horizontal gene transfer may have occurred among closely related microorganisms (Dandie et al., 2007; Jones et al., 2008) and we therefore cannot identify N2O reducers on the basis of nosZ sequence information alone. One approach to l ...
... phylogeny is generally in agreement with 16S rRNA gene phylogeny, horizontal gene transfer may have occurred among closely related microorganisms (Dandie et al., 2007; Jones et al., 2008) and we therefore cannot identify N2O reducers on the basis of nosZ sequence information alone. One approach to l ...
Properties of Mitotic and Meiotic Recombination in the
... Homologous recombination (HR) is an important mechanism for the repair of doublestranded DNA breaks (DSBs) in yeast (Symington et al., 2014) and in higher eukaryotes (Liang et al., 1998). Saccharomyces cerevisiae strains that lack HR are very sensitive to DNA damaging agents such as X-rays (Resnick ...
... Homologous recombination (HR) is an important mechanism for the repair of doublestranded DNA breaks (DSBs) in yeast (Symington et al., 2014) and in higher eukaryotes (Liang et al., 1998). Saccharomyces cerevisiae strains that lack HR are very sensitive to DNA damaging agents such as X-rays (Resnick ...
Array-based sequencing of filaggrin gene for
... with links to skin diseases, however it has a highly repetitive nucleotide sequence containing very limited stretches of unique nucleotides for precise mapping to reference genomes. Sequencing strategies using polymerase chain reaction (PCR) and conventional Sanger sequencing have been successful fo ...
... with links to skin diseases, however it has a highly repetitive nucleotide sequence containing very limited stretches of unique nucleotides for precise mapping to reference genomes. Sequencing strategies using polymerase chain reaction (PCR) and conventional Sanger sequencing have been successful fo ...
Optimal estimation of diffusion coefficients from single
... paramount for the precise understanding of these processes. Recent developments in fluorescent labels have made it possible to track diffusion of single molecules, e.g., proteins on biopolymers such as DNA [1–3] or microtubules [4–6], on surfaces [7], in lipid membranes [8–10], and inside cells [11– ...
... paramount for the precise understanding of these processes. Recent developments in fluorescent labels have made it possible to track diffusion of single molecules, e.g., proteins on biopolymers such as DNA [1–3] or microtubules [4–6], on surfaces [7], in lipid membranes [8–10], and inside cells [11– ...
Control of DNA excision efficiency in Paramecium
... within F1 progeny relative to F2 progeny demonstrates that Paramecium IES excision efficiency is sensitive either to a conjugation-specific trans-acting factor provided by the zygotic genome, or to homologous chromosome cross-talk. INTRODUCTION Programmed DNA rearrangements occur in a wide range of ...
... within F1 progeny relative to F2 progeny demonstrates that Paramecium IES excision efficiency is sensitive either to a conjugation-specific trans-acting factor provided by the zygotic genome, or to homologous chromosome cross-talk. INTRODUCTION Programmed DNA rearrangements occur in a wide range of ...
Chance and Necessity in Arthur Peacocke`s Scientific Work
... being used as a major approach for treatment of cancer. It was also known that radiation might be associated with the induction of growth malformations and cancer both from the studies of the Hiroshima and Nagasaki bomb survivors and also from on-going clinical studies. In hind sight, it seems reaso ...
... being used as a major approach for treatment of cancer. It was also known that radiation might be associated with the induction of growth malformations and cancer both from the studies of the Hiroshima and Nagasaki bomb survivors and also from on-going clinical studies. In hind sight, it seems reaso ...
Human RIF1 and protein phosphatase 1 stimulate DNA replication
... knockdown and exogenous overexpression of GFP-tagged RIF1 cDNA and mutant GFP-tagged RIF1 cDNA allows the authors to gain a transient look at RIF1 interactions in a human cell line. The authors show that the exogenously expressed GFP-RIF1 cDNA in their system does interact with all three isoforms of ...
... knockdown and exogenous overexpression of GFP-tagged RIF1 cDNA and mutant GFP-tagged RIF1 cDNA allows the authors to gain a transient look at RIF1 interactions in a human cell line. The authors show that the exogenously expressed GFP-RIF1 cDNA in their system does interact with all three isoforms of ...
Single-Nucleotide Polymorphism Mapping
... mutation of interest to be scored in a neutral phenotypic background. As a result, many markers can be assayed simultaneously, without worrying about genetic interactions. Second, SNPs between N2 and CB4856 are very dense. The average density is approximately one per kilobase of DNA (3), and to date ...
... mutation of interest to be scored in a neutral phenotypic background. As a result, many markers can be assayed simultaneously, without worrying about genetic interactions. Second, SNPs between N2 and CB4856 are very dense. The average density is approximately one per kilobase of DNA (3), and to date ...