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High-resolution melting analysis of the single nucleotide
High-resolution melting analysis of the single nucleotide

... the present study, 348 C. difficile strains with known PCR-ribotypes were investigated for respective single nucleotide polymorphisms (SNPs) within the proposed rpoB hot-spot region by using high-resolution melting (HRM) analysis. This method allows the detection of SNPs by comparing the altered mel ...
Phytopathology
Phytopathology

... various pathogens of potato, built on a solid support such as a nylon membrane or microscope slide, could be probed readily with labeled PCR products amplified from a potato sample. By using conserved primers to amplify common bacterial genome fragments from extracts of potato tubers that might cont ...
htp Pseudomonas aeruginosa AMANDA SWANEPOEL
htp Pseudomonas aeruginosa AMANDA SWANEPOEL

... In silico evidence has suggested that the pilin subunit gene flp is not part of the P. aeruginosa htp gene cluster thought to encode proteins involved in the synthesis, assembly and export of these pili. To determine the transcriptional organization of this gene cluster, total RNA from P. aeruginosa ...
Systematic and Applied Microbiology - digital
Systematic and Applied Microbiology - digital

... analyzed, produced tyramine. However, in both cases the production of biogenic amines was only evaluated in a decarboxylase agar medium where, sometimes, false-positive reactions have been described [10]. The results of our study demonstrate that the E. faecium strains analyzed actually possess a ge ...
Mechanisms of Transcription-Replication
Mechanisms of Transcription-Replication

... domain, restraining both processes prior to the direct encounter (3, 11, 14). The fraction of DNA knots appeared to be greater in those plasmids where replication collided with transcription head-on, rather than codirectionally, supporting the formation of such positively supercoiled domains (39). T ...
biology 160 laboratory objectives practical one
biology 160 laboratory objectives practical one

... BIOLOGY 160 LABORATORY OBJECTIVES PRACTICAL TWO ...
Introduction - Stellenbosch University
Introduction - Stellenbosch University

... 8. thickEnd - The ending position at which the feature is drawn thickly (for example the stop ...
LEADING ARTICLE Concurrent disruption of p16INK4a and
LEADING ARTICLE Concurrent disruption of p16INK4a and

... inhibiting phosphorylation of the retinoblastoma protein (Rb), while ARF prevents MDM2-mediated degradation of p53. By using a panel of PCR-based methods, we have examined the status of the p16INK4a, ARF and p53 genes in 123 cases of nonHodgkin’s lymphoma (NHL) at diagnosis. Alterations of one or mo ...
Lab 22 DNA-based Screening for Smallpox as a Bio
Lab 22 DNA-based Screening for Smallpox as a Bio

... naturally occurring case in Somalia in 1977. On May 8, 1980, the WHO declared smallpox as officially eradicated from the earth. Despite eradication of natural smallpox, many experts fear that this disease could be reintroduced into the population intentionally by terrorists. As immunity is thought t ...
Discovering the Distribution of Palindromic Sequences in the
Discovering the Distribution of Palindromic Sequences in the

... and quaternary structures of RNA are important to its function and expression; if an RNA molecule was mutated by a deletion or other mutation (particularly large mutations), RNA expression will be compromised as it may be too susceptible to enzymes or it may be unable to unwind during expression. Sm ...
Phylogenetic analysis of members of the genus Rickettsia using the
Phylogenetic analysis of members of the genus Rickettsia using the

... al., 1997). When the degree of infection was optimal, as estimated by Gimenez staining, rickettsiae were harvested and cultures were centrifuged at 12 000 g for 10 min, resuspended in medium and stored at k70 mC until nucleic acid purification was performed. Nucleic acid purification and PCR amplifi ...
Replication timing and transcriptional control: beyond
Replication timing and transcriptional control: beyond

... assays for replication [29]. There are only two mammalian loci for which both replication-timing switches and origin localization have been studied. At the mouse immunoglobulin IgH locus, new origins are activated in pre-B cells when the promoter region of this locus becomes transcriptionally active ...
Molecular Identification of Nematodes Manual
Molecular Identification of Nematodes Manual

... 10) With the P-200 set at 40.0ul and sticking the pipet tip into the master mix only a little bit, aliquot the master mix to each of the nema-smash PCR tubes, and pump the solution 5-6 times. Set the PCR tubes back at +4oC till you are all ready to place them all in the thermocycler. (No mineral oil ...
hag expression in Bacillus subtilis is both negatively
hag expression in Bacillus subtilis is both negatively

... In Bacillus subtilis, motility and chemotaxis require the expression of hag, which encodes flagellin. This gene is transcribed by the sD form of RNA polymerase and is regulated by a group of proteins called transition state regulators (TSRs). Our studies show that hag transcription is negatively reg ...
- Wiley Online Library
- Wiley Online Library

... assemble into a complex have shed some insight into these questions. Cohesin’s Smc1 and Smc3 subunits belong to the SMC (Structural Maintenance of Chromosomes) protein family, which has members present in all kingdoms of life. All SMC proteins appear to regulate chromosome morphology in some way or ...
arXiv:0708.2724v1 [cond-mat.other] 20 Aug 2007
arXiv:0708.2724v1 [cond-mat.other] 20 Aug 2007

... tailored to their specific condition(s). This latter goal is known as personalized medicine and would be a tremendous advancement in the medical field. Furthermore, the genomic information gathered could be used in the study of biology by, e.g., comparing genomes between species to find common prope ...
Epigenetics for behavioral ecologists
Epigenetics for behavioral ecologists

... genetic variants such that the behavior becomes constitutively expressed when it is adaptive (e.g., a more aggressive phenotype in a competitive, static environment), or more sensitive to environmental change when it is conditionally adaptive (a process referred to as “genetic accommodation”; sensu ...
Rh phenotype prediction by DNA typing and its
Rh phenotype prediction by DNA typing and its

... the most prevalent RHD-negative allele cannot yet be detected specifically. For the time being, we prefer therefore the term DNA typing rather than genotyping, when an RhD phenotype prediction by molecular techniques is attempted. We have summarized the molecular genetics of the RH genes according t ...
Supplementary Materials and methods (doc 154K)
Supplementary Materials and methods (doc 154K)

... plasmids was determined by counting the number of replicates growing on antibiotic- ...
MA112 Expresso® Rhamnose SUMO Cloning and
MA112 Expresso® Rhamnose SUMO Cloning and

... is designed for expression in E. coli, fusion clones constructed using this system can also be transferred into eukaryotic expression vectors for expression of SUMO-tagged proteins in mammalian or insect cells. The rhaP BAD promoter is a versatile tool for protein expression. In the absence of rhamn ...
The Rad50 Signature Motif: Essential to ATP Binding and
The Rad50 Signature Motif: Essential to ATP Binding and

... manganese ions for catalysis and form a DNAbinding groove that can accommodate a doublestranded DNA end.10 In vitro, Mre11 homologs exhibit 30 to 50 exonuclease activity on DNA substrates, as well as endonuclease activity on constrained structures such as hairpin ends.11 – 16 In every organism studi ...
Kein Folientitel
Kein Folientitel

... zygote ...
Stage-specific expressions of four different ribonuclease H genes in
Stage-specific expressions of four different ribonuclease H genes in

... 1998) was a generous gift from Dr. R. J. Crouch, NIH, USA.. These cells have a temperature sensitive mutation, because of which they can form colonies at 30oC but cannot support the growth at 42oC in the absence of functionally active RNase H. The ORFs of the different RNase H were amplified with hi ...
Classification of Centers for Disease Control Group Eugonic
Classification of Centers for Disease Control Group Eugonic

... organisms found in the oral cavity of dogs and cats, although systemic infections in humans and animals have also been reported (Ganière et al., 1995). Two biotypes can be distinguished based on the presence (EF-4a) or absence (EF-4b) of arginine dihydrolase activity (Holmes & Ahmed, 1981). In addi ...
biochemistry biochemistry laboratory experiments che 4350
biochemistry biochemistry laboratory experiments che 4350

... 2. Compute the average mass of the water delivered 10 times and the standard deviation. Show your work. Was the pipet precise? 3. Which of the pipettors that you used was the most accurate? 4. Using a computer, use a spreadsheet to make a plot of “volume dispensed” vs. the “Pipettor Setting” on a li ...
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Epigenomics

Epigenomics is the study of the complete set of epigenetic modifications on the genetic material of a cell, known as the epigenome. The field is analogous to genomics and proteomics, which are the study of the genome and proteome of a cell (Russell 2010 p. 217 & 230). Epigenetic modifications are reversible modifications on a cell’s DNA or histones that affect gene expression without altering the DNA sequence (Russell 2010 p. 475). Two of the most characterized epigenetic modifications are DNA methylation and histone modification. Epigenetic modifications play an important role in gene expression and regulation, and are involved in numerous cellular processes such as in differentiation/development and tumorigenesis (Russell 2010 p. 597). The study of epigenetics on a global level has been made possible only recently through the adaptation of genomic high-throughput assays (Laird 2010) and.
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