Towards Programmable Molecular Machines
... two states autonomously until all the fuel strands get used up. Conceptually, this makes it quite similar to an engine, though of course such molecular machines can not yet be used to perform useful work. There are several preliminary experiments for another DNA nano-machine called a DNA walker. A D ...
... two states autonomously until all the fuel strands get used up. Conceptually, this makes it quite similar to an engine, though of course such molecular machines can not yet be used to perform useful work. There are several preliminary experiments for another DNA nano-machine called a DNA walker. A D ...
Journal Club Pack - Circulation Research
... although we have previously shown that phenotypic switching of cultured SMC is dependent on Krupplelike factor (KLF)4. Objective: The goals of the present studies were to ascertain if (1) injury-induced repression of SM22α gene after vascular injury is mediated through KLF4 binding to the G/C Repres ...
... although we have previously shown that phenotypic switching of cultured SMC is dependent on Krupplelike factor (KLF)4. Objective: The goals of the present studies were to ascertain if (1) injury-induced repression of SM22α gene after vascular injury is mediated through KLF4 binding to the G/C Repres ...
The Mobile Genetic Element Alu in the Human Genome
... enetic material has been traditionally envisioned as relatively static with the exception of occasional, often deleterious mutations. The sequence DNA-toRNA-to-protein represented for many years the central dogma relating gene structure and function. Recently, the field of molecular genetics has pro ...
... enetic material has been traditionally envisioned as relatively static with the exception of occasional, often deleterious mutations. The sequence DNA-toRNA-to-protein represented for many years the central dogma relating gene structure and function. Recently, the field of molecular genetics has pro ...
Transgenic approaches for optogenetics Tim Murphy UBC.
... Cre is often expressed from a randomly integrated transgene, but very few insertion sites are known. Random transgene integration can disrupt an endogenous mouse gene and cause unanticipated side effects. Since hemizygous mice have a wildtype chromosome present, using hemizygous instead of homozygou ...
... Cre is often expressed from a randomly integrated transgene, but very few insertion sites are known. Random transgene integration can disrupt an endogenous mouse gene and cause unanticipated side effects. Since hemizygous mice have a wildtype chromosome present, using hemizygous instead of homozygou ...
Molecular markers in Brassica Rapa
... designed to cover the genomic sequence of BrCRTISO1 (BRAD gene ID: Bra031539) from the putative promoter region to the 500 bp downstream region from the stop codon (Supplementary Table 1). PCR conditions were as follows: 94 °C for 5 min; followed by cycles of 94 °C for 1 min, 58 °C for 1 min, and 72 ...
... designed to cover the genomic sequence of BrCRTISO1 (BRAD gene ID: Bra031539) from the putative promoter region to the 500 bp downstream region from the stop codon (Supplementary Table 1). PCR conditions were as follows: 94 °C for 5 min; followed by cycles of 94 °C for 1 min, 58 °C for 1 min, and 72 ...
Systematic Mutational Analysis of the Yeast ACT1 Gene.
... mutations lie at sites now known to be within a single subdomain of the folded protein (actl-1 changesa proline at residue 32 to leucine [or by convention, P32LI and actl-2 changes alanine at residue 58 to threonine [A58T]); the third is far removed from the first two (glutamate 259 to valine [E259V ...
... mutations lie at sites now known to be within a single subdomain of the folded protein (actl-1 changesa proline at residue 32 to leucine [or by convention, P32LI and actl-2 changes alanine at residue 58 to threonine [A58T]); the third is far removed from the first two (glutamate 259 to valine [E259V ...
Spatially ordered transcription of regulatory DNA in
... several non-overlapping fragments within each domain gave the same pattern of hybridization as the longer probes (Fig. 1). This makes it unlikely that the transcripts are detected by virtue of spurious hybridization to repeated sequences (No extensive repetitive sequences have been reported in the i ...
... several non-overlapping fragments within each domain gave the same pattern of hybridization as the longer probes (Fig. 1). This makes it unlikely that the transcripts are detected by virtue of spurious hybridization to repeated sequences (No extensive repetitive sequences have been reported in the i ...
A natural chimeric yeast containing genetic material from three species
... not shown) and thus it is not likely that this yeast is a hybrid. This finding was supported by Southern hybridization of genomic IFO 1802 DNA digested with six different restriction enzymes and probed with IFO 1802 MET2. Invariably, only one distinct signal was found (data not shown). The MET2 DNA ...
... not shown) and thus it is not likely that this yeast is a hybrid. This finding was supported by Southern hybridization of genomic IFO 1802 DNA digested with six different restriction enzymes and probed with IFO 1802 MET2. Invariably, only one distinct signal was found (data not shown). The MET2 DNA ...
Pierre MADL Div. of Material Sciences Dep
... familial, population, or evolutionary level, genes are always central to their ...
... familial, population, or evolutionary level, genes are always central to their ...
Two enhancer regions in the mouse En-2 locus
... et al., 1991). In the adult, En-2 is strongly expressed in cells of the internal granular layer of the cerebellum. No expression is detected in the neighbouring Purkinje cells, whereas weak expression is detectable in cells of the molecular layer (Davis et al., 1988; Davis and Joyner, 1988; K. Mille ...
... et al., 1991). In the adult, En-2 is strongly expressed in cells of the internal granular layer of the cerebellum. No expression is detected in the neighbouring Purkinje cells, whereas weak expression is detectable in cells of the molecular layer (Davis et al., 1988; Davis and Joyner, 1988; K. Mille ...
Induction of XIST expression from the human active
... The XIST gene is the only gene known to be expressed exclusively from the inactive X chromosome (8) and is localised to the smallest interval of the X chromosome required in cis for inactivation to occur (9,10). The XIST RNA, which is not translated, remains in the nucleus where it is associated wit ...
... The XIST gene is the only gene known to be expressed exclusively from the inactive X chromosome (8) and is localised to the smallest interval of the X chromosome required in cis for inactivation to occur (9,10). The XIST RNA, which is not translated, remains in the nucleus where it is associated wit ...
Graph-based Methods for the Design of DNA - TUBdok
... DNA computing is a rapidly evolving field utilizing DNA molecules instead of silicon-based electronic units to perform calculations. The reliability of such computations strongly depends on the DNA sequences that represent units of information. Recently, the thermodynamic constraints, based on the f ...
... DNA computing is a rapidly evolving field utilizing DNA molecules instead of silicon-based electronic units to perform calculations. The reliability of such computations strongly depends on the DNA sequences that represent units of information. Recently, the thermodynamic constraints, based on the f ...
The genomic landscape of meiotic crossovers and gene
... result from unknown rearrangements or repeats. This is especially critical for Ler and Cvi, where genome information is more limited than for the reference accession Col. Figure 2A shows an example where three haplotypes appear to be present at a single locus when the short read data of Ler are alig ...
... result from unknown rearrangements or repeats. This is especially critical for Ler and Cvi, where genome information is more limited than for the reference accession Col. Figure 2A shows an example where three haplotypes appear to be present at a single locus when the short read data of Ler are alig ...
Modular Stitching To Image Single
... To put this work into context: while macroscopic aspects of electrophoresis (separation efficiency and its dependence on electric field and gel type) have been understood for a long time,4 the present new observations of microscopic chain dynamics at the single-molecule level encourage us to compare to ...
... To put this work into context: while macroscopic aspects of electrophoresis (separation efficiency and its dependence on electric field and gel type) have been understood for a long time,4 the present new observations of microscopic chain dynamics at the single-molecule level encourage us to compare to ...
DNA Evolution 3.0 Administrator Guide
... The DNA Evolution Controller is software that runs on a networked Linux appliance. This software can run on a physical server (X Series, N Series) or on a virtual server (M Series). This core software component is included in all DNA Evolution products and is the foundation of the solution. The appl ...
... The DNA Evolution Controller is software that runs on a networked Linux appliance. This software can run on a physical server (X Series, N Series) or on a virtual server (M Series). This core software component is included in all DNA Evolution products and is the foundation of the solution. The appl ...
The Large Loop Repair and Mismatch Repair Pathways
... Plasmids containing his4 alleles with varying length DNA sequence insertions were used to replace the wild-type HIS4 chromosomal sequence in AS13. Each plasmid was constructed by annealing two complementary oligonucleotides and inserting the oligos into the Sal I site in HIS4 on pDN9 (Nag et al. 198 ...
... Plasmids containing his4 alleles with varying length DNA sequence insertions were used to replace the wild-type HIS4 chromosomal sequence in AS13. Each plasmid was constructed by annealing two complementary oligonucleotides and inserting the oligos into the Sal I site in HIS4 on pDN9 (Nag et al. 198 ...
Structure-Function Analysis of the Conserved Histone Chaperone
... Chromatin structure is crucial to regulate access to the genome for processes such as transcription, recombination, DNA repair, and DNA replication. Spt6, a key factor involved in regulating chromatin struct ...
... Chromatin structure is crucial to regulate access to the genome for processes such as transcription, recombination, DNA repair, and DNA replication. Spt6, a key factor involved in regulating chromatin struct ...
Read the Nobel Lecture
... First we needed an assay – a way to detect if telomere elongation was happening. The first assay we tried explored whether a piece of DNA that included a telomere would incorporate DNA precursors more readily than a piece of DNA containing non-telomeric sequences. The idea was that if there was an ...
... First we needed an assay – a way to detect if telomere elongation was happening. The first assay we tried explored whether a piece of DNA that included a telomere would incorporate DNA precursors more readily than a piece of DNA containing non-telomeric sequences. The idea was that if there was an ...
i. Genetics
... of all cells in all living organisms The kind of cell which is formed, (muscle, blood, nerve etc.) is controlled by DNA The kind of organism which is produced (giraffe, herring, human, etc.) is controlled by DNA ...
... of all cells in all living organisms The kind of cell which is formed, (muscle, blood, nerve etc.) is controlled by DNA The kind of organism which is produced (giraffe, herring, human, etc.) is controlled by DNA ...
The Arabidopsis RAD51 paralogs RAD51B, RAD51D and XRCC2
... to DNA damage by bleomycin. RNA-Seq transcriptome analysis supported the idea that the triple mutant showed DNA damage similar to that caused by bleomycin. On bleomycin treatment, many genes were altered in the wild-type but not in the triple mutant, suggesting that the RAD51 paralogs have roles in ...
... to DNA damage by bleomycin. RNA-Seq transcriptome analysis supported the idea that the triple mutant showed DNA damage similar to that caused by bleomycin. On bleomycin treatment, many genes were altered in the wild-type but not in the triple mutant, suggesting that the RAD51 paralogs have roles in ...
NOCARDIA sp. INDONESIAN VOLCANIC SOIL DESAK GEDE SRI ANDAYANI , ELIN YULINAH SUKANDAR
... Microorganisms that grow in extreme environments such as volcanic area have way of self-defense in order to survive its life. Nocardia sp. is one genus of this group which can find in volcanic soil will appear to be the most promising source of the future antibiotics. Ways in which microorganisms am ...
... Microorganisms that grow in extreme environments such as volcanic area have way of self-defense in order to survive its life. Nocardia sp. is one genus of this group which can find in volcanic soil will appear to be the most promising source of the future antibiotics. Ways in which microorganisms am ...
E.coli
... • E. coli as host: Many eukaryotic genes and their control sequences have been isolated and analyzed using gene cloning techniques based on E. coli as host. • Eukaryotic Vectors: However, many applications of genetic engineering (see Section J) require vectors for the expression of foreign genes in ...
... • E. coli as host: Many eukaryotic genes and their control sequences have been isolated and analyzed using gene cloning techniques based on E. coli as host. • Eukaryotic Vectors: However, many applications of genetic engineering (see Section J) require vectors for the expression of foreign genes in ...
The nucleotide sequence of Saccharomyces cerevisiae
... availability of an ordered library of large-insert bacterial or viral genomic clones for the organism under study. The generation of these large insert libraries, and the location of each clone on a genome map, is a laborious and time-consuming process. In an effort to overcome these problems, sever ...
... availability of an ordered library of large-insert bacterial or viral genomic clones for the organism under study. The generation of these large insert libraries, and the location of each clone on a genome map, is a laborious and time-consuming process. In an effort to overcome these problems, sever ...
1 Defining the epigenetic mechanism of asymmetric cell division of
... strain Sj4 cells by quantifying zygote formation among four granddaughter progeny of a single cell. Luckily, unlike S. pombe, S. japonicus cell pairs of opposite mating type readily mate without requiring further growth and starvation on Pombe Minimal Adenine (PMA) medium. We exploited this property ...
... strain Sj4 cells by quantifying zygote formation among four granddaughter progeny of a single cell. Luckily, unlike S. pombe, S. japonicus cell pairs of opposite mating type readily mate without requiring further growth and starvation on Pombe Minimal Adenine (PMA) medium. We exploited this property ...