Structural Studies on the Dosage Compensation Complex from
... In Drosophila, dosage compensation (Figure: 1.1) is achieved by a twofold up-regulation of gene expression on the single X chromosome mediated by a dosage compensation complex (DCC). It has been shown that this complex is spread all over the Drosophila male X chromosome. As a consequence the express ...
... In Drosophila, dosage compensation (Figure: 1.1) is achieved by a twofold up-regulation of gene expression on the single X chromosome mediated by a dosage compensation complex (DCC). It has been shown that this complex is spread all over the Drosophila male X chromosome. As a consequence the express ...
DNA Polyacrylamide Gel Electrophoresis
... 9. Use a Pasteur pipette or a syringe to flush out the wells once more with 1x TBE. Mix the DNA samples with the appropriate amount of gelloading buffer. Load the mixture into the wells using a micropipette equipped with a drawn-out plastic tip. Do not attempt to expel all of the sample from the loa ...
... 9. Use a Pasteur pipette or a syringe to flush out the wells once more with 1x TBE. Mix the DNA samples with the appropriate amount of gelloading buffer. Load the mixture into the wells using a micropipette equipped with a drawn-out plastic tip. Do not attempt to expel all of the sample from the loa ...
Archives of Microbiology 167:
... Abstract A Tn5-1063-derived mutant of Nostoc punctiforme strain ATCC 29133 was unable to fix N2 in air although it reduced acetylene in the absence of O2. Mutant strain UCD 307 formed cells morphologically similar to heterocysts, but it failed to synthesize the characteristic heterocyst glycolipids. ...
... Abstract A Tn5-1063-derived mutant of Nostoc punctiforme strain ATCC 29133 was unable to fix N2 in air although it reduced acetylene in the absence of O2. Mutant strain UCD 307 formed cells morphologically similar to heterocysts, but it failed to synthesize the characteristic heterocyst glycolipids. ...
Highly precise and developmentally programmed genome
... expression but is destroyed at each sexual cycle, while the germline micronucleus (MIC) undergoes meiosis and transmits its genome to the zygotic nucleus. New MICs and MACs of sexual progeny differentiate from copies of the zygotic nucleus and extensive genome rearrangements take place in the new MA ...
... expression but is destroyed at each sexual cycle, while the germline micronucleus (MIC) undergoes meiosis and transmits its genome to the zygotic nucleus. New MICs and MACs of sexual progeny differentiate from copies of the zygotic nucleus and extensive genome rearrangements take place in the new MA ...
pdf
... propagated by infection of other individuals. This may be a natural means for evolving new strains of viruses. One of the most striking examples is the acquisition and modification of a protooncogene, such as cellular c-src, by a retrovirus to generate a modified, transforming form of the gene, call ...
... propagated by infection of other individuals. This may be a natural means for evolving new strains of viruses. One of the most striking examples is the acquisition and modification of a protooncogene, such as cellular c-src, by a retrovirus to generate a modified, transforming form of the gene, call ...
Canadian Journal of Microbiology
... identification of isolates, and direct 16S rDNA PCR amplification followed by whole-community fingerprinting (TRFLP) and by construction of a bacterial clone library was used. The results clearly indicated that a wide range of bacteria from diverse phylogenetic affiliation, mainly γ-Proteobacteria a ...
... identification of isolates, and direct 16S rDNA PCR amplification followed by whole-community fingerprinting (TRFLP) and by construction of a bacterial clone library was used. The results clearly indicated that a wide range of bacteria from diverse phylogenetic affiliation, mainly γ-Proteobacteria a ...
Clinical and Molecular Aspects of Diseases of Mitochondrial DNA
... Despite its small size, mtDNA encodes 22 transfer RNAs, 2 ribosomal RNAs and 13 polypeptides which are required for OXPHOS. Although the number 1000 is widely quoted as the mtDNA complement of a typical cell, there is in fact considerable variation in the mtDNA copy number among different cell types ...
... Despite its small size, mtDNA encodes 22 transfer RNAs, 2 ribosomal RNAs and 13 polypeptides which are required for OXPHOS. Although the number 1000 is widely quoted as the mtDNA complement of a typical cell, there is in fact considerable variation in the mtDNA copy number among different cell types ...
Direct Deletion Analysis in Two Duchenne Muscular Dystrophy
... We determined the segregation of the STR (CA)n alleles that were located within the deletion-prone regions 5’DYS-II, 07A, 5’-7n4, STR 44, STR 45, STR 49, and STR 50. The PCR products of the alleles were analyzed by superimposing the traces of the proband to that of her relatives. The haplotypes of t ...
... We determined the segregation of the STR (CA)n alleles that were located within the deletion-prone regions 5’DYS-II, 07A, 5’-7n4, STR 44, STR 45, STR 49, and STR 50. The PCR products of the alleles were analyzed by superimposing the traces of the proband to that of her relatives. The haplotypes of t ...
Analysis and nucleotide sequence of an origin of DNA replication in
... pWH1266 and transformants were scored on LB plates containing 100/~gAp/ml. Competency is observed from the early log phase to the stationary phase. A maximum of competency is found in the late log phase of growth with about 6 × l0 s transformants per 500 ng of plasmid DNA. This procedure is slightly ...
... pWH1266 and transformants were scored on LB plates containing 100/~gAp/ml. Competency is observed from the early log phase to the stationary phase. A maximum of competency is found in the late log phase of growth with about 6 × l0 s transformants per 500 ng of plasmid DNA. This procedure is slightly ...
Lampbrush Chromosomes of the Chicken
... and somewhat more difficult to handle. In 1-3-mm chicken oocytes, the GVs usually range from 200 to 400 l~m in diameter. The quality of chicken LBC preparations tends to be more variable from animal to animal and even among similar sized oocytes from the same animal relative to the newt. The spread ...
... and somewhat more difficult to handle. In 1-3-mm chicken oocytes, the GVs usually range from 200 to 400 l~m in diameter. The quality of chicken LBC preparations tends to be more variable from animal to animal and even among similar sized oocytes from the same animal relative to the newt. The spread ...
BioACTS Quarter THREE
... a nucleic acid is called a nucleotide. Nucleotides are made up of THREE parts—a phosphate group, a sugar (deoxyribose) and one of four nitrogen bases. Diagram I Draw and label ONE nucleotide using the highlighters below your entry task. ...
... a nucleic acid is called a nucleotide. Nucleotides are made up of THREE parts—a phosphate group, a sugar (deoxyribose) and one of four nitrogen bases. Diagram I Draw and label ONE nucleotide using the highlighters below your entry task. ...
BLOTTING.142
... blot, WB, northern blot, NB) that employ similar principles, but using protein or RNA, have later been named in reference to Edwin Southern's name. Dr. Azhar Chishti ...
... blot, WB, northern blot, NB) that employ similar principles, but using protein or RNA, have later been named in reference to Edwin Southern's name. Dr. Azhar Chishti ...
Screening of a Specific Point Mutation in Tumor Suppressor p53
... The point mutation at a specific site (the third base of codon 249 of exon 7) in the p53 gene was not found in the 8 hepatocellular carcinoma samples from Korean patients. This result is quite different from the report on Chinese and South African patients that showed the point mutations at the same ...
... The point mutation at a specific site (the third base of codon 249 of exon 7) in the p53 gene was not found in the 8 hepatocellular carcinoma samples from Korean patients. This result is quite different from the report on Chinese and South African patients that showed the point mutations at the same ...
here - Genomes Unzipped
... identical to those reported by Li et al., though we found a few differences between the sites reported by Li et al. [1] and our analysis. For example, Li et al.[1] report an RDD site at chromosome 4, position 3,9141,595; as far as we can tell, all of the mismatching bases at that site have a low seq ...
... identical to those reported by Li et al., though we found a few differences between the sites reported by Li et al. [1] and our analysis. For example, Li et al.[1] report an RDD site at chromosome 4, position 3,9141,595; as far as we can tell, all of the mismatching bases at that site have a low seq ...
![Crystal structures of -[Ru(phen)2dppz]2+ 1 with oligonucleotides](http://s1.studyres.com/store/data/023790436_1-6c83654c03a0a35fc50303cc056eabf6-300x300.png)
Crystal structures of -[Ru(phen)2dppz]2+ 1 with oligonucleotides
... Description of the structures Complex between 1 and d(CCGGTACCGG) This self-complementary sequence crystallizes to give a symmetrical duplex with a stoichiometry of three cations of 1 per duplex, or 1.5 cations of 1 per decamer strand. All the nucleic acid strands are equivalent in the crystal latt ...
... Description of the structures Complex between 1 and d(CCGGTACCGG) This self-complementary sequence crystallizes to give a symmetrical duplex with a stoichiometry of three cations of 1 per duplex, or 1.5 cations of 1 per decamer strand. All the nucleic acid strands are equivalent in the crystal latt ...
Amplification of 16S rRNA Genes from Frankia Strains in Root
... contaminating bacterial genomes was quite low. Phenolic compounds present in actinorhizal nodule homogenates normally turn bright red or orange because of spontaneous oxidation in air and can complicate the purification of proteins or DNA. Adding ascorbate to the TE buffer and washing the hyphal clu ...
... contaminating bacterial genomes was quite low. Phenolic compounds present in actinorhizal nodule homogenates normally turn bright red or orange because of spontaneous oxidation in air and can complicate the purification of proteins or DNA. Adding ascorbate to the TE buffer and washing the hyphal clu ...
Distortion of quantitative genomic and expression
... regarding reproducibility of these techniques have been raised by cross-validation studies in different laboratories (1–5). Strategies to mitigate variability in the results obtained from replicate studies have focused on standardizing technical factors, such as array production, RNA synthesis, labe ...
... regarding reproducibility of these techniques have been raised by cross-validation studies in different laboratories (1–5). Strategies to mitigate variability in the results obtained from replicate studies have focused on standardizing technical factors, such as array production, RNA synthesis, labe ...
Intelligent Icons: Integrating Lite-Weight Data Mining
... The three files in the example are ASCII text files, each of which contains approximately 16,000 base pairs of mitochondrial DNA. Here we used string edit distance as suggested in [15] to measure the distance between file names, and Euclidean distance to measure the distance between the file icons ( ...
... The three files in the example are ASCII text files, each of which contains approximately 16,000 base pairs of mitochondrial DNA. Here we used string edit distance as suggested in [15] to measure the distance between file names, and Euclidean distance to measure the distance between the file icons ( ...
Painting the target around the matching profile
... (i.e. alleles not associated with the sample) are sometimes detected, a phenomenon known as allelic ‘drop-in’ A further complication is that evidentiary samples are often mixtures of DNA from more than one person. It can be difficult to tell how many contributors there were to a mixed sample (Paolet ...
... (i.e. alleles not associated with the sample) are sometimes detected, a phenomenon known as allelic ‘drop-in’ A further complication is that evidentiary samples are often mixtures of DNA from more than one person. It can be difficult to tell how many contributors there were to a mixed sample (Paolet ...
Gene quantification using real-time quantitative PCR
... DNA copy number measurements are important in determining the extent of genomic imbalance that underlies most malignancies. There are numerous techniques available for measuring DNA copy number in tumors; each method has specific advantages and disadvantages. Chromosomal CGH can detect imbalances ac ...
... DNA copy number measurements are important in determining the extent of genomic imbalance that underlies most malignancies. There are numerous techniques available for measuring DNA copy number in tumors; each method has specific advantages and disadvantages. Chromosomal CGH can detect imbalances ac ...
the association of chloroplast dna with photosynthetic membrane
... in the electron microscope they appear as in Figs. 6-8. They are bound by a membrane to which adhere varying numbers of grana lamellae. The number of grana lamellae seen in a single section will vary according to the section plane. The grana are partially disrupted due to the isolation in the 3-5 mM ...
... in the electron microscope they appear as in Figs. 6-8. They are bound by a membrane to which adhere varying numbers of grana lamellae. The number of grana lamellae seen in a single section will vary according to the section plane. The grana are partially disrupted due to the isolation in the 3-5 mM ...
Manipulating Yeast Genome Using Plasmid Vectors. In: Gene Expression Technology.
... we discuss below require that one be able to find the rare cells that have undergone a recombination or loss event to remove the plasmid DNA sequences. Unfortunately, the L YS2 gene is quite large, and contains sites within the coding sequence for many of the most common enzymes used in plasmid cons ...
... we discuss below require that one be able to find the rare cells that have undergone a recombination or loss event to remove the plasmid DNA sequences. Unfortunately, the L YS2 gene is quite large, and contains sites within the coding sequence for many of the most common enzymes used in plasmid cons ...
SNP
... Bifurcated duplex with a free 5’ end There is at least one base overlapping the strand that has a free 5’ end and the strand that is annealed to the template The enzyme cleaves the strand with a free 5’ end when one or more overlapping bases are detected ...
... Bifurcated duplex with a free 5’ end There is at least one base overlapping the strand that has a free 5’ end and the strand that is annealed to the template The enzyme cleaves the strand with a free 5’ end when one or more overlapping bases are detected ...
Journal of Molecular Biology
... tested. phoR strain CE1220 was transformed with pJY50 and. in caont.rast to thcb parental phoR strain, all 25 CmR transformants t)ested did not produce alkaline phosphatase constit,utively and were resistant to phage T(‘45. Therefore ~~JP50 also carries the phoR gene. In order to determine a restric ...
... tested. phoR strain CE1220 was transformed with pJY50 and. in caont.rast to thcb parental phoR strain, all 25 CmR transformants t)ested did not produce alkaline phosphatase constit,utively and were resistant to phage T(‘45. Therefore ~~JP50 also carries the phoR gene. In order to determine a restric ...
Introduction to RNA sequencing
... pronounced effect on gene expression • e.g. Drug treated vs. untreated cell line • e.g. Wild type versus knock out mice ...
... pronounced effect on gene expression • e.g. Drug treated vs. untreated cell line • e.g. Wild type versus knock out mice ...