Chapter 21
... An insertion sequence is a transposon that codes for the enzyme(s) needed for transposition flanked by short inverted terminal repeats. The target site at which a transposon is inserted is duplicated during the insertion process to form two repeats in direct orientation at the ends of the transposon ...
... An insertion sequence is a transposon that codes for the enzyme(s) needed for transposition flanked by short inverted terminal repeats. The target site at which a transposon is inserted is duplicated during the insertion process to form two repeats in direct orientation at the ends of the transposon ...
Imprinting and Seed Development
... duce seeds asexually by apomixis, which exists in several different forms (Nogler, 1984; Koltunow, 1993; Bicknell and Koltunow, 2004). During gametophytic apomixis, the megagametophyte develops from an unreduced megaspore or from a somatic cell inside the ovule. The diploid embryo then develops from ...
... duce seeds asexually by apomixis, which exists in several different forms (Nogler, 1984; Koltunow, 1993; Bicknell and Koltunow, 2004). During gametophytic apomixis, the megagametophyte develops from an unreduced megaspore or from a somatic cell inside the ovule. The diploid embryo then develops from ...
Comparison of DNA Sequences with Protein Sequences
... to join some clearly related superfamilies (W.R.P, manuscript in preparation; the PIR39b database is available for downloading from ftp. virginia.edu:/pub/fasta). Two sequences from each of 46 families of proteins were used for these tests. The cDNA sequences, and their corresponding open reading fr ...
... to join some clearly related superfamilies (W.R.P, manuscript in preparation; the PIR39b database is available for downloading from ftp. virginia.edu:/pub/fasta). Two sequences from each of 46 families of proteins were used for these tests. The cDNA sequences, and their corresponding open reading fr ...
SALSA MLPA KIT ME003-A1 Tumor suppressor-3 - MRC
... islands upstream of genes for which an altered methylation status in one or more types of tumours has been reported in literature. In case interesting results are obtained by users, it is possible to develop methylation kits specific for a certain tumour in collaboration with MRC-Holland. Interpreta ...
... islands upstream of genes for which an altered methylation status in one or more types of tumours has been reported in literature. In case interesting results are obtained by users, it is possible to develop methylation kits specific for a certain tumour in collaboration with MRC-Holland. Interpreta ...
Using comparative genomic hybridization to
... study, we propose the use of array-based comparative genomic hybridization (aCGH) in order to identify orthologous genes with high sequence divergence. Here we discuss experimental design, statistical power, success rate, sources of variation and potential confounding factors. We used a spotted PCR ...
... study, we propose the use of array-based comparative genomic hybridization (aCGH) in order to identify orthologous genes with high sequence divergence. Here we discuss experimental design, statistical power, success rate, sources of variation and potential confounding factors. We used a spotted PCR ...
How dormant origins promote complete genome replication
... With these considerations in mind, we recently modelled the behaviour of origin activation within a single 250 kb origin cluster [41]. Origins were assigned a certain initiation probability per unit time and were then activated stochastically during S phase (Figure 4a). Model parameters (mean origin ...
... With these considerations in mind, we recently modelled the behaviour of origin activation within a single 250 kb origin cluster [41]. Origins were assigned a certain initiation probability per unit time and were then activated stochastically during S phase (Figure 4a). Model parameters (mean origin ...
Translation
... → polypeptide chain is released from tRNA at the P site and it is bound by peptide bond to the amino acid carried by the tRNA at the A site → at the same time tRNA carrying prolonged polypeptide chain is shifted from the A site to the P site and now free tRNA is shifted from the P site to E site → t ...
... → polypeptide chain is released from tRNA at the P site and it is bound by peptide bond to the amino acid carried by the tRNA at the A site → at the same time tRNA carrying prolonged polypeptide chain is shifted from the A site to the P site and now free tRNA is shifted from the P site to E site → t ...
unit-2 genetics of prokaryotes and eukaryotic
... the host's DNA (Figure 2.8). When the virus enters a cell, a virus-encoded enzyme called lambda integrase is synthesized. This enzyme catalyzes a recombination process that begins when several molecules of the integrase protein bind tightly to a specific DNA sequence on the circular bacteriophage ch ...
... the host's DNA (Figure 2.8). When the virus enters a cell, a virus-encoded enzyme called lambda integrase is synthesized. This enzyme catalyzes a recombination process that begins when several molecules of the integrase protein bind tightly to a specific DNA sequence on the circular bacteriophage ch ...
Translation
... A specific initiator tRNA carrying methionine is required for the initiation of translation. Initiation of translation: binding of the initiator tRNA together with other initiator factors to the small ribosomal subunit → complex binds to the 5´end of mRNA (it recognizes the cap) → it slides along mR ...
... A specific initiator tRNA carrying methionine is required for the initiation of translation. Initiation of translation: binding of the initiator tRNA together with other initiator factors to the small ribosomal subunit → complex binds to the 5´end of mRNA (it recognizes the cap) → it slides along mR ...
Translation
... → polypeptide chain is released from tRNA at the P site and it is bound by peptide bond to the amino acid carried by the tRNA at the A site → at the same time tRNA carrying prolonged polypeptide chain is shifted from the A site to the P site and now free tRNA is shifted from the P site to E site → t ...
... → polypeptide chain is released from tRNA at the P site and it is bound by peptide bond to the amino acid carried by the tRNA at the A site → at the same time tRNA carrying prolonged polypeptide chain is shifted from the A site to the P site and now free tRNA is shifted from the P site to E site → t ...
Altering gene expression by aminocoumarins: the role of DNA
... cause severe inhibition of recA transcription [20]. This effect is likely mediated by the known inhibition of the GyrB subunit. To verify this assumption, a strain expressing a non-susceptible GyrB enzyme was analysed. A mutated gyrB gene (Ile102Ser, Arg144Ile) [23,24] was transduced into strain HG0 ...
... cause severe inhibition of recA transcription [20]. This effect is likely mediated by the known inhibition of the GyrB subunit. To verify this assumption, a strain expressing a non-susceptible GyrB enzyme was analysed. A mutated gyrB gene (Ile102Ser, Arg144Ile) [23,24] was transduced into strain HG0 ...
SYBR Green with low ROX
... high-speed hot-start Taq, optimal reaction buffer, dNTP, stabilizing agents, ROX reference dye and SYBR Green I dye. RbTaq™ Fast qPCR 2X PreMIX (SYBR Green with low ROX) is a repebody (Rb) mediated hot start method of Taq DNA polymerase. In the hot start method, engineered Taq DNA polymerse is combi ...
... high-speed hot-start Taq, optimal reaction buffer, dNTP, stabilizing agents, ROX reference dye and SYBR Green I dye. RbTaq™ Fast qPCR 2X PreMIX (SYBR Green with low ROX) is a repebody (Rb) mediated hot start method of Taq DNA polymerase. In the hot start method, engineered Taq DNA polymerse is combi ...
Mechanistic Comparison of High-Fidelity and Error
... Discovered in the early 1970s, DNA polymerase β (Pol β) was the first mammalian DNA repair polymerase to be characterized. Its relatively small size (39 kD) and the fact that it lacks an exonuclease proofreading activity make it a tractable system for studying the mechanism by which fidelity is achi ...
... Discovered in the early 1970s, DNA polymerase β (Pol β) was the first mammalian DNA repair polymerase to be characterized. Its relatively small size (39 kD) and the fact that it lacks an exonuclease proofreading activity make it a tractable system for studying the mechanism by which fidelity is achi ...
Significant enhancement of fatty acid composition in seeds of the
... mutations in the FAD2 genes of Arabidopsis and Camelina plants was confirmed by DNA sequencing. Sequence analyses of DNA from leaf and seed samples (Data Set S2) confirmed multiple mutations over multiple generations at each of the three target sites in each of the three different FAD2 gene types pr ...
... mutations in the FAD2 genes of Arabidopsis and Camelina plants was confirmed by DNA sequencing. Sequence analyses of DNA from leaf and seed samples (Data Set S2) confirmed multiple mutations over multiple generations at each of the three target sites in each of the three different FAD2 gene types pr ...
Lab (3) Report: Population Genetics
... Discussion: Why did we get such results? Reiterating my genotypic results which are present in Figure 1 of the Results section, I am homozygous recessive as I have only a single band. There is no difference between my digested and undigested samples as the effect of HaeIII was the same. This is con ...
... Discussion: Why did we get such results? Reiterating my genotypic results which are present in Figure 1 of the Results section, I am homozygous recessive as I have only a single band. There is no difference between my digested and undigested samples as the effect of HaeIII was the same. This is con ...
Replication of plasmids with the p15A origin in Shewanella
... England BioLabs) to verify that the correct sequence had been amplified. DNA manipulations ...
... England BioLabs) to verify that the correct sequence had been amplified. DNA manipulations ...
Transcriptional Repression of Hox Genes by C. elegans HP1/HPL
... temperatures [15–17]. hpl-1, in contrast to hpl-2, does not have visible effects on C. elegans development at different temperatures, however, hpl-1 acts redundantly with hpl-2 to control larval development, somatic gonad development and vulval cell fate determination [17]. Our previous study reveal ...
... temperatures [15–17]. hpl-1, in contrast to hpl-2, does not have visible effects on C. elegans development at different temperatures, however, hpl-1 acts redundantly with hpl-2 to control larval development, somatic gonad development and vulval cell fate determination [17]. Our previous study reveal ...
lac
... Transcriptional Regulation of Operons -Regulatory sequences adjacent to an operon determine whether it is transcribed or not. -Regulatory sequences are primarily ‘operators’ (repressor binding sequences). Can also include activator binding sequences. -Regulatory proteins work with regulatory sequen ...
... Transcriptional Regulation of Operons -Regulatory sequences adjacent to an operon determine whether it is transcribed or not. -Regulatory sequences are primarily ‘operators’ (repressor binding sequences). Can also include activator binding sequences. -Regulatory proteins work with regulatory sequen ...
Recombinant DNA technology and molecular cloning
... can be isolated and amplified. One method of isolation and amplification of a gene of interest is to clone the gene by inserting it into another DNA molecule that serves as a vehicle or vector that can be replicated in living cells. When these two DNAs of different origin are combined, the result is ...
... can be isolated and amplified. One method of isolation and amplification of a gene of interest is to clone the gene by inserting it into another DNA molecule that serves as a vehicle or vector that can be replicated in living cells. When these two DNAs of different origin are combined, the result is ...
network - bioinf leipzig
... core, and bottom layers. TFs within a layer are highly interconnected and share similar properties. TFs of the different layers regulate distinct sets of targets genes. The three layers are also connected by a central skeleton, a feed-forward structure that utilizes the TFs of the top layer to regul ...
... core, and bottom layers. TFs within a layer are highly interconnected and share similar properties. TFs of the different layers regulate distinct sets of targets genes. The three layers are also connected by a central skeleton, a feed-forward structure that utilizes the TFs of the top layer to regul ...
A-10484A SNPs. Mutations and DNA Sequence
... between two heterozygous alleles is important for accurate detection. We measured this signal balance for each of the two alleles of the six possible heterozygote combinations and found an average signal ratio of 1:1.6. The allele calling accuracy for a 10-plex reaction is >99%. Comparisons were don ...
... between two heterozygous alleles is important for accurate detection. We measured this signal balance for each of the two alleles of the six possible heterozygote combinations and found an average signal ratio of 1:1.6. The allele calling accuracy for a 10-plex reaction is >99%. Comparisons were don ...
HbF inducers
... ● Histone deacetylase (HDAC) enzymes determine deacetylation of histone tails, causing chromatin condensation and repression of transcription ● Many experimental evidences have indicated that inhibition of the activity of HDAC causes an increased HbF synthesis ...
... ● Histone deacetylase (HDAC) enzymes determine deacetylation of histone tails, causing chromatin condensation and repression of transcription ● Many experimental evidences have indicated that inhibition of the activity of HDAC causes an increased HbF synthesis ...
Bcmb625-XistPaper-26apr07clp
... - Is fluorescence an accurate enough measure of transcriptional state? - What about the converse experiment express just the A-repeat region… - Experiments using truncations of the delta-A construct…. - Does the Xist domain co-localize with the nuclear structure components? - What is the nature of ...
... - Is fluorescence an accurate enough measure of transcriptional state? - What about the converse experiment express just the A-repeat region… - Experiments using truncations of the delta-A construct…. - Does the Xist domain co-localize with the nuclear structure components? - What is the nature of ...
Non-invasive prenatal assessment of trisomy 21 by multiplexed
... Peripheral venous blood samples (5–10 mL) were collected into tubes containing EDTA. A plasma sample would be accepted for analysis if it was collected before invasive obstetric procedures, was harvested within six hours of venepuncture, was at least 2 mL in volume, and was not haemolysed. The prosp ...
... Peripheral venous blood samples (5–10 mL) were collected into tubes containing EDTA. A plasma sample would be accepted for analysis if it was collected before invasive obstetric procedures, was harvested within six hours of venepuncture, was at least 2 mL in volume, and was not haemolysed. The prosp ...
Biosynthesis of the Antibiotic Nonribosomal Peptide Penicillin in
... into the Nrp product15, 23. NRPS modules incorporate both the standard 20 amino acids, as well as hundreds of non-proteinogenic amino acids, including D-enantiomers24. Thus by combining different modules together it should be possible make chimeric NRPS enzymes that produce thousands of novel Nrp mo ...
... into the Nrp product15, 23. NRPS modules incorporate both the standard 20 amino acids, as well as hundreds of non-proteinogenic amino acids, including D-enantiomers24. Thus by combining different modules together it should be possible make chimeric NRPS enzymes that produce thousands of novel Nrp mo ...