Original Article Genetic and epigenetic association studies suggest
... Abstract: Given strong evidence implicating an important role of altered microRNA expression in cancer initiation and progression, the genes responsible for microRNA biogenesis may also play a role in tumorigenesis. Exportin-5 (XPO5) is responsible for exporting pre-miRNAs through the nuclear membra ...
... Abstract: Given strong evidence implicating an important role of altered microRNA expression in cancer initiation and progression, the genes responsible for microRNA biogenesis may also play a role in tumorigenesis. Exportin-5 (XPO5) is responsible for exporting pre-miRNAs through the nuclear membra ...
Mutations: The Effect On Phenotype
... something, a receptor on the tongue bindsi a specific chemical in food. When the chemical binds to the receptor, a signal is sent to the brain where it is interpreted as a particular taste—sour, bitter, sweet, salty or umami. Recently, the gene that codes for the receptor that binds PTC was identifi ...
... something, a receptor on the tongue bindsi a specific chemical in food. When the chemical binds to the receptor, a signal is sent to the brain where it is interpreted as a particular taste—sour, bitter, sweet, salty or umami. Recently, the gene that codes for the receptor that binds PTC was identifi ...
DNA Sequence Capture and Enrichment by Microarray Followed by
... DNA was nebulized to yield fragments from 250 bp to approximately 1 kb in size, and a 22mer linker was added to both ends. The DNA fragments were then hybridized to the NF1 sequence-capture array for 3 days. After hybridization and stringent washing, the DNA fragments remaining on the array (mostly ...
... DNA was nebulized to yield fragments from 250 bp to approximately 1 kb in size, and a 22mer linker was added to both ends. The DNA fragments were then hybridized to the NF1 sequence-capture array for 3 days. After hybridization and stringent washing, the DNA fragments remaining on the array (mostly ...
Genetic mapping of Theobroma cacao (Malvaceae - Funpec-RP
... alleles, probably deriving from specific individual mutations that are characterized by nonpigmentation of the leaves (Bartley, 2005). A similar effect was observed in F2 progenies, resulting from self-fertilization of F1 plants from Pa 121 x SIC 802 and Pa 121 x Pa 169 (Yamada et al., 1982). One fo ...
... alleles, probably deriving from specific individual mutations that are characterized by nonpigmentation of the leaves (Bartley, 2005). A similar effect was observed in F2 progenies, resulting from self-fertilization of F1 plants from Pa 121 x SIC 802 and Pa 121 x Pa 169 (Yamada et al., 1982). One fo ...
lecture notes
... occur during the movement of the nuclei to the periphery. An hour after fertilization the embryo is composed of about 800 nuclei forming a monolayer around the periphery of the egg. During the next 30 min the nuclei undergo another 3 rounds of division to form nuclear cleavage cycle 14 embryos conta ...
... occur during the movement of the nuclei to the periphery. An hour after fertilization the embryo is composed of about 800 nuclei forming a monolayer around the periphery of the egg. During the next 30 min the nuclei undergo another 3 rounds of division to form nuclear cleavage cycle 14 embryos conta ...
The Non-LTR Retrotransposon Rex3 from the Fish Xiphophorus is
... Introduction Fishes make up more than half of the 48,000 species of living vertebrates. They should therefore possess genetic tools for speciation-associated genome evolution. Transposons may be one of the factors fulfilling this function due to their ability to move within genomes, to generate muta ...
... Introduction Fishes make up more than half of the 48,000 species of living vertebrates. They should therefore possess genetic tools for speciation-associated genome evolution. Transposons may be one of the factors fulfilling this function due to their ability to move within genomes, to generate muta ...
Nat. Struct. Biol. 8, 192-194.
... viewed as entry points for transcription factors which, once bound, can interact with the transcription complex by looping out intervening sequences9. More recently, enhancer-bound transcription factors have been shown tethered to proteins at the promoter region that are involved in histone modifica ...
... viewed as entry points for transcription factors which, once bound, can interact with the transcription complex by looping out intervening sequences9. More recently, enhancer-bound transcription factors have been shown tethered to proteins at the promoter region that are involved in histone modifica ...
Analysis of the histone H3 gene family in Arabidopsis and
... Histones are major components of chromatin, the protein–DNA complex involved in DNA packaging and transcriptional regulation. Histone genes have been extensively investigated at the genome level in animal systems and have been classified as replication dependent, replication independent or tissue sp ...
... Histones are major components of chromatin, the protein–DNA complex involved in DNA packaging and transcriptional regulation. Histone genes have been extensively investigated at the genome level in animal systems and have been classified as replication dependent, replication independent or tissue sp ...
Smchd1 regulates a subset of autosomal genes subject to
... equivalence between XX females and XY males [1]. Other examples of monoallelic expression include: (a) genomic imprinting where the parental origin of the alleles of specific genes or gene clusters are epigenetically marked during gametogenesis to be expressed exclusively from either the maternally ...
... equivalence between XX females and XY males [1]. Other examples of monoallelic expression include: (a) genomic imprinting where the parental origin of the alleles of specific genes or gene clusters are epigenetically marked during gametogenesis to be expressed exclusively from either the maternally ...
Molecular analysis of the structure and expression of the RH... individuals with D--, Dc-, and DCw- gene complexes
... exons 4-6 and exons 9-10, respectively. The absence of the 23-kb fragment after hybridization with exon 7 or exon 8 suggested either a deletion of the relevant regions of the RHCE gene in the DC- gene complex or the presence of an unusual band comigrating with the 19 kb D-specific fragment. The exon ...
... exons 4-6 and exons 9-10, respectively. The absence of the 23-kb fragment after hybridization with exon 7 or exon 8 suggested either a deletion of the relevant regions of the RHCE gene in the DC- gene complex or the presence of an unusual band comigrating with the 19 kb D-specific fragment. The exon ...
DNA Base Sequence Homology in Rhizoctonia solani Kuihn: Inter
... AG-10 (12). Some of these anastomosis groups are further subdivided based on pathogenicity (AG-1, AG-2), colony morphology (AG-1), thiamine requirement (AG-2, AG-9), and/or DNA homology (AG-4, AG-6) into intraspecific groups (ISG) (11). The ISG is derived by combining anastomosis group affiliation w ...
... AG-10 (12). Some of these anastomosis groups are further subdivided based on pathogenicity (AG-1, AG-2), colony morphology (AG-1), thiamine requirement (AG-2, AG-9), and/or DNA homology (AG-4, AG-6) into intraspecific groups (ISG) (11). The ISG is derived by combining anastomosis group affiliation w ...
Missense mutations in the PAX6 gene in aniridia.
... do possess distinct DNA binding. However, when both subdomains are linked as in the PAX6 protein, they negatively regulate the other's function, that is, some mutations in the Nterminal subdomain not only loose a function controlled by the N-terminal subdomain but also gain a function controlled by ...
... do possess distinct DNA binding. However, when both subdomains are linked as in the PAX6 protein, they negatively regulate the other's function, that is, some mutations in the Nterminal subdomain not only loose a function controlled by the N-terminal subdomain but also gain a function controlled by ...
Missense mutations in the PAX6 gene in aniridia.
... do possess distinct DNA binding. However, when both subdomains are linked as in the PAX6 protein, they negatively regulate the other's function, that is, some mutations in the Nterminal subdomain not only loose a function controlled by the N-terminal subdomain but also gain a function controlled by ...
... do possess distinct DNA binding. However, when both subdomains are linked as in the PAX6 protein, they negatively regulate the other's function, that is, some mutations in the Nterminal subdomain not only loose a function controlled by the N-terminal subdomain but also gain a function controlled by ...
Identification of the Minus-Dominance Gene Ortholog in
... DNA gel blot analysis of GpMID: Southern blot analysis was done following the protocols of Nishii et al. (2003) modified to use the nonradioisotope detection system outlined below. Restriction enzyme digests (6 mg) of genomic DNA were separated by 1.0% agarose gel electrophoresis and transferred ont ...
... DNA gel blot analysis of GpMID: Southern blot analysis was done following the protocols of Nishii et al. (2003) modified to use the nonradioisotope detection system outlined below. Restriction enzyme digests (6 mg) of genomic DNA were separated by 1.0% agarose gel electrophoresis and transferred ont ...
Embryo and Endosperm Inherit Distinct Chromatin
... quiescence follows fertilization. In this study, we show that the zygote remains relatively quiescent and that the embryo can undergo several divisions in the absence of de novo transcription, thus relying on deposited maternal products. By contrast, the endosperm strictly requires de novo transcrip ...
... quiescence follows fertilization. In this study, we show that the zygote remains relatively quiescent and that the embryo can undergo several divisions in the absence of de novo transcription, thus relying on deposited maternal products. By contrast, the endosperm strictly requires de novo transcrip ...
Clamp loader structure predicts the architecture of DNA polymerase
... in the absence of ATP [13]. ATP binding to γ may separate δ′ from δ, allowing δ to bind β and open the ring. How do the above observations fit with the γ complex structure? The β interactive element site on the δ subunit is contained within the amino-terminal domain (see Figure 3a). The β interactiv ...
... in the absence of ATP [13]. ATP binding to γ may separate δ′ from δ, allowing δ to bind β and open the ring. How do the above observations fit with the γ complex structure? The β interactive element site on the δ subunit is contained within the amino-terminal domain (see Figure 3a). The β interactiv ...
letter Widespread aneuploidy revealed by DNA microarray expression profiling
... expression ratio of each gene as chromosome # chromosome # a function of its chromosomal location, we noted an expres- Fig. 3 Selection for aneuploidy in rnr1∆ and rps24a∆/rps24a∆ mutants. a, Slow-growing rnr1∆ (sector 2) or sion bias in a 56-ORF region on rps24a∆/rps24a∆ (sector 4) cells or their i ...
... expression ratio of each gene as chromosome # chromosome # a function of its chromosomal location, we noted an expres- Fig. 3 Selection for aneuploidy in rnr1∆ and rps24a∆/rps24a∆ mutants. a, Slow-growing rnr1∆ (sector 2) or sion bias in a 56-ORF region on rps24a∆/rps24a∆ (sector 4) cells or their i ...
Lecture 2
... – Fragment DNA (with cross-linked proteins still attached). – ChIP: enrichment of TF-associated fragments. – Reverse cross-linking and purify DNA. Identification of enriched DNA regions: – Clone, sequence and map back to the genome – For anticipated binding sites, PCR with specific primers – Amplify ...
... – Fragment DNA (with cross-linked proteins still attached). – ChIP: enrichment of TF-associated fragments. – Reverse cross-linking and purify DNA. Identification of enriched DNA regions: – Clone, sequence and map back to the genome – For anticipated binding sites, PCR with specific primers – Amplify ...
Microbial Diversity in Prince Edward County`s Soil Microbiome
... thousands of types of microbial bacteria that inhabit many different habitats and environments. New types of microbial bacteria are often discovered, so there is a lot about it that scientists are unsure of. Microbial bacteria play an important role in the ecosystems they inhabit, as well as indicat ...
... thousands of types of microbial bacteria that inhabit many different habitats and environments. New types of microbial bacteria are often discovered, so there is a lot about it that scientists are unsure of. Microbial bacteria play an important role in the ecosystems they inhabit, as well as indicat ...
Construction of plant BAC libraries This document
... 12. On a clean Qtray cover on a workbench, reconstruct the gel by placing the unstained evennumbered pieces between their flanking odd-numbered stained pieces (FIGURE 2d). Extend the incisions at 125 kb and 300 kb on each stained gel piece into adjacent unstained (even-numbered) gel piece. Place th ...
... 12. On a clean Qtray cover on a workbench, reconstruct the gel by placing the unstained evennumbered pieces between their flanking odd-numbered stained pieces (FIGURE 2d). Extend the incisions at 125 kb and 300 kb on each stained gel piece into adjacent unstained (even-numbered) gel piece. Place th ...
How to determine whether a strain will undergo senescence. Background
... to grow indefinitely. Vegetatively propogated cultures are virtually immortal, with a potential for unlimited growth. Deleterious mutant genes and gene combinations are expected eventually to accumulate and slow or terminate growth even in normal growing, nonsenescent cultures. Occasional outcrossin ...
... to grow indefinitely. Vegetatively propogated cultures are virtually immortal, with a potential for unlimited growth. Deleterious mutant genes and gene combinations are expected eventually to accumulate and slow or terminate growth even in normal growing, nonsenescent cultures. Occasional outcrossin ...
Explaining the Likelihood Ratio in DNA Mixture Interpretation
... reporting on DNA evidence. There are several reasons for its relative unpopularity. First, outside of the DNA area, most forensic science disciplines do not yet have a LR available. Moreover, within DNA identification, forensic analysts sometimes find the LR hard to explain. However, all DNA match s ...
... reporting on DNA evidence. There are several reasons for its relative unpopularity. First, outside of the DNA area, most forensic science disciplines do not yet have a LR available. Moreover, within DNA identification, forensic analysts sometimes find the LR hard to explain. However, all DNA match s ...
Explaining the Likelihood Ratio in DNA Mixture
... reporting on DNA evidence. There are several reasons for its relative unpopularity. First, outside of the DNA area, most forensic science disciplines do not yet have a LR available. Moreover, within DNA identification, forensic analysts sometimes find the LR hard to explain. However, all DNA match s ...
... reporting on DNA evidence. There are several reasons for its relative unpopularity. First, outside of the DNA area, most forensic science disciplines do not yet have a LR available. Moreover, within DNA identification, forensic analysts sometimes find the LR hard to explain. However, all DNA match s ...
BWS - Beckwith-Wiedemann Syndrome support
... chromosome 11. U represents an unmethylated switch (the red traffic light shows that the gene is off). M represents a methylated switch (the green traffic light shows that the gene is on). How does the genetic cause of BWS correlate with the symptoms of BWS? Both reduced CDKN1C activity and increas ...
... chromosome 11. U represents an unmethylated switch (the red traffic light shows that the gene is off). M represents a methylated switch (the green traffic light shows that the gene is on). How does the genetic cause of BWS correlate with the symptoms of BWS? Both reduced CDKN1C activity and increas ...
Current Second Tier and Future Applications of Gene Sequencing in
... • Labeled primers, laser detects specific fluorescent tags so we can now combine nucleotides in one tube A, C, G, T • Can automate set-up • Uses PCR to make DNA ...
... • Labeled primers, laser detects specific fluorescent tags so we can now combine nucleotides in one tube A, C, G, T • Can automate set-up • Uses PCR to make DNA ...