Sex-linked dosage-sensitive modifiers as imprinting
... of dominance modification (Fisher, 1928). As such, the phenomena are most easily treated within the confines of models that invoke the activity of modifier genes (imprinting genes) on particular loci (imprinted or modified genes). While these models, as initially proposed (Sapienza, 1989), have been ...
... of dominance modification (Fisher, 1928). As such, the phenomena are most easily treated within the confines of models that invoke the activity of modifier genes (imprinting genes) on particular loci (imprinted or modified genes). While these models, as initially proposed (Sapienza, 1989), have been ...
Using an Alu Insertion Polymorphism to Study Human
... Use and Lab Safety: The materials supplied are for use with the method described in this kit only. Use of this kit presumes and requires prior knowledge of basic methods of gel electrophoresis and staining of DNA. Individuals should use this kit only in accordance with prudent laboratory safety prec ...
... Use and Lab Safety: The materials supplied are for use with the method described in this kit only. Use of this kit presumes and requires prior knowledge of basic methods of gel electrophoresis and staining of DNA. Individuals should use this kit only in accordance with prudent laboratory safety prec ...
Overexpression of the catalytic subunit of DNA polymerase results in
... of the Tfam gene in mouse produces a severe depletion of mtDNA, thereby abolishing oxidative phosphorylation, whereas a reduction in gene dosage reduces mtDNA copy number and produces mitochondrial defects in the heart (Larsson et al. 1998). In Drosophila, the catalytic subunit of mitochondrial DNA ...
... of the Tfam gene in mouse produces a severe depletion of mtDNA, thereby abolishing oxidative phosphorylation, whereas a reduction in gene dosage reduces mtDNA copy number and produces mitochondrial defects in the heart (Larsson et al. 1998). In Drosophila, the catalytic subunit of mitochondrial DNA ...
Deconstructing the Genome: DNA at High Resolution
... that cut DNA, ligases that join two molecules together, and polymerases that synthesize new DNA strands. The most important uncatalyzed biochemical reaction is hybridization: the binding together of two DNA strands with complementary nucleotide sequences. Hybridization results from the natural prope ...
... that cut DNA, ligases that join two molecules together, and polymerases that synthesize new DNA strands. The most important uncatalyzed biochemical reaction is hybridization: the binding together of two DNA strands with complementary nucleotide sequences. Hybridization results from the natural prope ...
Evidence for allelism of the recessive insertional
... the gene product is essential in other processes or there might be other gene(s) within the deletion that are recessive mutated, not involved in limb formation, but essential for normal development. At present we are attempting to detect transcript alterations in the Xt and/or add mice using DNA pro ...
... the gene product is essential in other processes or there might be other gene(s) within the deletion that are recessive mutated, not involved in limb formation, but essential for normal development. At present we are attempting to detect transcript alterations in the Xt and/or add mice using DNA pro ...
Identifying a Novel Isoform of the AZIN1 Gene by Combining High
... reading frame that would change the terminus of the subsequent protein from Ser-Asp-Glu-Asp-stop to PheArg-stop. Follow-up studies could validate this finding on the protein level and then measure gene expression of this new isoform in various tissues, subjects, and time-points. Moreover, the method ...
... reading frame that would change the terminus of the subsequent protein from Ser-Asp-Glu-Asp-stop to PheArg-stop. Follow-up studies could validate this finding on the protein level and then measure gene expression of this new isoform in various tissues, subjects, and time-points. Moreover, the method ...
The Development of a Genetic Marker for Resistance to Fescue
... polymerase chain reaction (PCR) followed by Restriction Fragment Length Polymorphism (RFLP) analysis. Phenotypic information on steers included serum prolactin concentrations and body weight measured monthly over April, May, and June of 2006 and 2007. Hair coat scores were recorded for each steer in ...
... polymerase chain reaction (PCR) followed by Restriction Fragment Length Polymorphism (RFLP) analysis. Phenotypic information on steers included serum prolactin concentrations and body weight measured monthly over April, May, and June of 2006 and 2007. Hair coat scores were recorded for each steer in ...
CHARACTERlZATION OF THE ~ 0 CHONDRIA . L DNA MOLECULE
... mitochondrid replication and transcription are not known, comparative analyses of the controI region from various mamindian species have identified regions of sequence consensus and possible functionai importance (Ciayton, 199la; 199 1b). These include the conserved sequence blocks [CSB) (Walberg an ...
... mitochondrid replication and transcription are not known, comparative analyses of the controI region from various mamindian species have identified regions of sequence consensus and possible functionai importance (Ciayton, 199la; 199 1b). These include the conserved sequence blocks [CSB) (Walberg an ...
Phylogenetic Affinity of Mitochondria of Euglena
... citrate) 0.1% SDS at 55°C for 15 min each. Cloning of COI-encoding genomic fragment. Mitochondrial DNA was digested with Sau3AI or Taq I and cloned into pBluescript KS− (Stratagene). Several hundred clones were screened with the same PCR-amplified L. tarentolae COI gene fragment as used in the South ...
... citrate) 0.1% SDS at 55°C for 15 min each. Cloning of COI-encoding genomic fragment. Mitochondrial DNA was digested with Sau3AI or Taq I and cloned into pBluescript KS− (Stratagene). Several hundred clones were screened with the same PCR-amplified L. tarentolae COI gene fragment as used in the South ...
fragile x dna testing: a guide for physicians and families
... Among the millions of DNA fragments that result are some containing the section of the FMR1 gene with the CGG repeat. When there is no mutation, these FMR1 fragments are of a single known size. If a mutation is present, the fragments are longer. Furthermore, fragments from FMR1 genes that are methyl ...
... Among the millions of DNA fragments that result are some containing the section of the FMR1 gene with the CGG repeat. When there is no mutation, these FMR1 fragments are of a single known size. If a mutation is present, the fragments are longer. Furthermore, fragments from FMR1 genes that are methyl ...
Nucleotide Sequence Preservation of Human
... sequence comparisons of the D-loop region of unrelated normal humans (5, 21). Three additional between-individual differences outside the Dloop region of mtDNA were identified in this study. Substitution of cytidine for thymidine at L-strand positions 9698 and 9725 was found in all clones containing ...
... sequence comparisons of the D-loop region of unrelated normal humans (5, 21). Three additional between-individual differences outside the Dloop region of mtDNA were identified in this study. Substitution of cytidine for thymidine at L-strand positions 9698 and 9725 was found in all clones containing ...
Damage Control: The Pleiotropy of DNA Repair Genes
... tified in many organisms. For example, the human XPA protein is believed to mediate recognition of intrastrand crosslinks (reviewed in Wood 1996), and Escherichia coli MutS (and the eukaryotic Msh proteins) binds specifically to base pair mismatches and small insertion/deletion mutations (reviewed i ...
... tified in many organisms. For example, the human XPA protein is believed to mediate recognition of intrastrand crosslinks (reviewed in Wood 1996), and Escherichia coli MutS (and the eukaryotic Msh proteins) binds specifically to base pair mismatches and small insertion/deletion mutations (reviewed i ...
Functional analysis of plastid DNA replication origins in tobacco by
... pea only two have been proposed due to the lack of an Inverted Repeat. oriA was localised within the rRNA operon, oriB downstream of the trnN gene. As the rRNA operon shows a high degree of conservation between different species, it appears possible that oriA is also located at this site in other sp ...
... pea only two have been proposed due to the lack of an Inverted Repeat. oriA was localised within the rRNA operon, oriB downstream of the trnN gene. As the rRNA operon shows a high degree of conservation between different species, it appears possible that oriA is also located at this site in other sp ...
187-192. Control of transcription by Pontin and Reptin
... Pontin and Reptin are two closely related members of the AAA+ family of DNA helicases. They play roles in diverse cellular processes, including the response to DNA double-strand breaks and the control of gene expression. The two proteins share residence in different multi-protein complexes, such as ...
... Pontin and Reptin are two closely related members of the AAA+ family of DNA helicases. They play roles in diverse cellular processes, including the response to DNA double-strand breaks and the control of gene expression. The two proteins share residence in different multi-protein complexes, such as ...
Chapter 2 Assignment: Genetics
... letter to the editor: A genetically modified (GM) rice known as “golden rice” has been produced. The rice is somewhat yellow in colour due to the same pigment that gives carrots and sweet potatoes their colour. This pigment is beta-carotene, which can be converted by the body to vitamin A. Vitamin A ...
... letter to the editor: A genetically modified (GM) rice known as “golden rice” has been produced. The rice is somewhat yellow in colour due to the same pigment that gives carrots and sweet potatoes their colour. This pigment is beta-carotene, which can be converted by the body to vitamin A. Vitamin A ...
FX 8 - ACMG
... Description of Methods: Direct DNA analysis of the CGG repeat in the fragile X mental retardation 1 gene (FMR1) was performed by Triplet Repeat Primed PCR followed by capillary electrophoresis. Southern blot analysis, when performed, uses the StB12.3 probe and genomic DNA digested with EagI/EcoRI. ...
... Description of Methods: Direct DNA analysis of the CGG repeat in the fragile X mental retardation 1 gene (FMR1) was performed by Triplet Repeat Primed PCR followed by capillary electrophoresis. Southern blot analysis, when performed, uses the StB12.3 probe and genomic DNA digested with EagI/EcoRI. ...
Foundations in Microbiology
... complementary tails for gene splicing. This palindrome is cut by Aci I. (2) The sticky ends can be used to join DNA from different organisms by cutting it with the same restriction enzyme, ensuring that both fragments have two complementary ends. This palindrome is cut by Taq I. Copyright © McGraw-H ...
... complementary tails for gene splicing. This palindrome is cut by Aci I. (2) The sticky ends can be used to join DNA from different organisms by cutting it with the same restriction enzyme, ensuring that both fragments have two complementary ends. This palindrome is cut by Taq I. Copyright © McGraw-H ...
AP Biology Chapter 18 Review Answer Section
... cAMP. When the cAMP increases in concentration, it binds to CAP. Which of the following would you expect would then be a measurable effect? a. decreased concentration of the lac enzymes b. increased concentration of the trp enzymes c. decreased binding of the RNA polymerase to sugar metabolism-relat ...
... cAMP. When the cAMP increases in concentration, it binds to CAP. Which of the following would you expect would then be a measurable effect? a. decreased concentration of the lac enzymes b. increased concentration of the trp enzymes c. decreased binding of the RNA polymerase to sugar metabolism-relat ...
Bridging the transgenerational gap with epigenetic memory
... revealed that exposure of parents to environmental stimuli could affect the phenotype of several generations of descendants, suggesting that changes in epigenetic modifications induced by environmental stimuli might be passed on to descendants via the gametes. Importantly, the field has recently mad ...
... revealed that exposure of parents to environmental stimuli could affect the phenotype of several generations of descendants, suggesting that changes in epigenetic modifications induced by environmental stimuli might be passed on to descendants via the gametes. Importantly, the field has recently mad ...
A Rapid Chromosome Mapping Method for Cloned Fragments of Yeast DNA.
... of homology. We have used all of these methods successfully. No matter which method is used, however, the primary problem is the detection of the integrants among autonomous transformants and the verification that the integration has occurred at the locus one wants to map. As will be shown, the simp ...
... of homology. We have used all of these methods successfully. No matter which method is used, however, the primary problem is the detection of the integrants among autonomous transformants and the verification that the integration has occurred at the locus one wants to map. As will be shown, the simp ...
PDF
... of a focused light beam [12, 13]. Soon thereafter, magnetic tweezers were invented to allow the manipulation of paramagnetic beads using a gradient of magnetic field [14]. The two techniques have been developing head to head to achieve nowadays a remarkable level of sophistication. They have their o ...
... of a focused light beam [12, 13]. Soon thereafter, magnetic tweezers were invented to allow the manipulation of paramagnetic beads using a gradient of magnetic field [14]. The two techniques have been developing head to head to achieve nowadays a remarkable level of sophistication. They have their o ...
DNA Duplication Associated with Charcot-Marie-Tooth Disease Type 1A. Lupski, et al., 1991 Cell, Vol. 66, 219-232, July 26, 1991,
... Vance et al., 1991); the CMTlB locus maps to human chromosome 1 (region q23-q25) (Bird et al., 1982); and a third type is unlinked to both the CMTlA and CMTIB loci (Chance et al., 1990). These studies provide the basis for isolating the disease gene(s) by virtue of map position. Positional cloning e ...
... Vance et al., 1991); the CMTlB locus maps to human chromosome 1 (region q23-q25) (Bird et al., 1982); and a third type is unlinked to both the CMTlA and CMTIB loci (Chance et al., 1990). These studies provide the basis for isolating the disease gene(s) by virtue of map position. Positional cloning e ...
X inactivation Xplained
... see how to discriminate between different models if not by analysis of molecular components. XCI starts with the accumulation of Xist along the future inactive X chromosome. Recent work indicates that Xist up-regulation is the consequence of sex-specific induction of Xist transcription on the future ...
... see how to discriminate between different models if not by analysis of molecular components. XCI starts with the accumulation of Xist along the future inactive X chromosome. Recent work indicates that Xist up-regulation is the consequence of sex-specific induction of Xist transcription on the future ...