gene technology extra qs with mark scheme
... The table shows a sequence of bases from the mRNA coding for insulin. Complete the table to show the sequence of bases you would expect in the single-stranded DNA copy. ...
... The table shows a sequence of bases from the mRNA coding for insulin. Complete the table to show the sequence of bases you would expect in the single-stranded DNA copy. ...
[PDF]
... 310Luc and 310Luc/Sg2/2 mice (data not shown). Similarly increased expression (, twofold) of luciferase was present in the CNS and heart of 310Luc/Sp42/2 mice compared with wildtype 310Luc mice (P ,0.02) (Fig. 3B). The exception was in the eye of 310Luc/Sp42/2 animals, in which expression levels wer ...
... 310Luc and 310Luc/Sg2/2 mice (data not shown). Similarly increased expression (, twofold) of luciferase was present in the CNS and heart of 310Luc/Sp42/2 mice compared with wildtype 310Luc mice (P ,0.02) (Fig. 3B). The exception was in the eye of 310Luc/Sp42/2 animals, in which expression levels wer ...
The Origin of the Jingwei Gene and the Complex Modular Structure
... resulting from random insertion of a retrosequence into a region devoid of regulatory sequences. Further molecular population genetic analysis, however, revealed strong purifying selection, as shown by the near limitation of nucleotide polymorphism to silent sites (Long and Langley 1993). This gene ...
... resulting from random insertion of a retrosequence into a region devoid of regulatory sequences. Further molecular population genetic analysis, however, revealed strong purifying selection, as shown by the near limitation of nucleotide polymorphism to silent sites (Long and Langley 1993). This gene ...
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... • Examine the effect of “mutations” on motifs • Examine the effect of motif location within promoter • Examine the effect of motif combinations, distances within a combination • More? ...
... • Examine the effect of “mutations” on motifs • Examine the effect of motif location within promoter • Examine the effect of motif combinations, distances within a combination • More? ...
MHC Recognition MHC Recognition DuPont Essay Rough Draft
... What does MHC do to mice? What is MHC? It controls their lifestyle from birth to mating and even to raising pups of their own. Communal nesting is observed in mice as a means of efficiently raising their young. By communally nursing, parents are able to care for their young with less if not no effor ...
... What does MHC do to mice? What is MHC? It controls their lifestyle from birth to mating and even to raising pups of their own. Communal nesting is observed in mice as a means of efficiently raising their young. By communally nursing, parents are able to care for their young with less if not no effor ...
Dosage Compensation: Transcription-Level Regulation of X
... would have separate regulatory elements for each structural gene on the X chromosome. Each of these elements may have either a stimulating or inhibitory action while their overall effect would be one of repression of transcription. Needless to say, such compensation would be difficult to demonstrate ...
... would have separate regulatory elements for each structural gene on the X chromosome. Each of these elements may have either a stimulating or inhibitory action while their overall effect would be one of repression of transcription. Needless to say, such compensation would be difficult to demonstrate ...
Clustering_PartII_2012
... HS + EtOH analysis (added 2 replicates of a new conditions): Only 1618 genes were DE (at any of the models) at FDR of 5% ??? Why so few when 3157 met this cutoff when HS was analyzed alone? baySeq paper: harder to call DE with “more complex” models ...
... HS + EtOH analysis (added 2 replicates of a new conditions): Only 1618 genes were DE (at any of the models) at FDR of 5% ??? Why so few when 3157 met this cutoff when HS was analyzed alone? baySeq paper: harder to call DE with “more complex” models ...
Unearthing the Roles of Imprinted Genes in the Placenta
... precocious deaths in animals or humans [9]. Following fertilization, a single-cell zygote forms a multicellular organism comprised of more than 200 different cell types [14,15]. The development of lineage-specific cells begins with the differentiation of the trophoblast lineage and the inner cell mas ...
... precocious deaths in animals or humans [9]. Following fertilization, a single-cell zygote forms a multicellular organism comprised of more than 200 different cell types [14,15]. The development of lineage-specific cells begins with the differentiation of the trophoblast lineage and the inner cell mas ...
Paralogous gene conversion, allelic divergence of attacin genes
... CSR-2 which were resistant and susceptible to BmNPV infection respectively were used in this study. Expression profiles of B. mori genes were analyzed using microarray technique and results revealed that the immune response genes including attacin were selectively up regulated in virus invaded midgu ...
... CSR-2 which were resistant and susceptible to BmNPV infection respectively were used in this study. Expression profiles of B. mori genes were analyzed using microarray technique and results revealed that the immune response genes including attacin were selectively up regulated in virus invaded midgu ...
Stressed Memories - Journal of Neuroscience
... overlap in content was achieved by parallelizing studied pictures and movies based on content features, both depicting real-life, emotionally salient stimuli. To be more precise, the movies used in the stress condition contained, for example, male to male and male to female violence, mutilations, an ...
... overlap in content was achieved by parallelizing studied pictures and movies based on content features, both depicting real-life, emotionally salient stimuli. To be more precise, the movies used in the stress condition contained, for example, male to male and male to female violence, mutilations, an ...
... recurrent circuits. The entorhinal cortex in the parahippocampal region is crucially involved in the acquisition, consolidation and retrieval of long-term memory traces for which working memory operations are essential2. Here we show that individual neurons from layer V of the entorhinal cortex—whic ...
Identification of genes required for hydrogenase activity in
... for identifying important genes involved in specific cellular pathways and processes [12]. Mutants were generated by transforming the Arg7 gene into C. reinhardtii strain CC425, which is an arginine auxotroph. The Arg7 gene is randomly incorporated into the C. reinhardtii genome and disrupts small s ...
... for identifying important genes involved in specific cellular pathways and processes [12]. Mutants were generated by transforming the Arg7 gene into C. reinhardtii strain CC425, which is an arginine auxotroph. The Arg7 gene is randomly incorporated into the C. reinhardtii genome and disrupts small s ...
Disruption of mCry2 restores circadian rhythmicity in mPer2 mutant
... maximum at CT6 in Per2Brdm1/mCry2−/− mutants (Fig. 2a). Thus, circadian expression of mPer1 in Per2Brdm1 mutants is rescued by inactivation of mCry2. A similar rescue was observed for mCry1 expression: both amplitude and timing of mCry1 oscillation in Per2Brdm1/ mCry2−/− mutant animals was not signi ...
... maximum at CT6 in Per2Brdm1/mCry2−/− mutants (Fig. 2a). Thus, circadian expression of mPer1 in Per2Brdm1 mutants is rescued by inactivation of mCry2. A similar rescue was observed for mCry1 expression: both amplitude and timing of mCry1 oscillation in Per2Brdm1/ mCry2−/− mutant animals was not signi ...
DNA Prokaryote Transcription Steps (updated February 2013)
... formation of the replication bubble. Once the RNA strand exceeds 10 nucleotides (bases), TFIIB drops off. Additional phosphorylation of the CTD of the RNA pol II by TFIIH pushes the polymerase into the elongation phase. TFIIH drops off. TFIID stays behind to form a new pre-initiation complex. TFIIF ...
... formation of the replication bubble. Once the RNA strand exceeds 10 nucleotides (bases), TFIIB drops off. Additional phosphorylation of the CTD of the RNA pol II by TFIIH pushes the polymerase into the elongation phase. TFIIH drops off. TFIID stays behind to form a new pre-initiation complex. TFIIF ...
AGRA: analysis of gene ranking algorithms
... Next, BCS categories for the gene symbol are calculated. If the gene symbol is associated with only one protein, its BCS is identical to the protein’s one. When the symbol is associated with more than one protein, the average values of the frequencies in each category are calculated. In the final st ...
... Next, BCS categories for the gene symbol are calculated. If the gene symbol is associated with only one protein, its BCS is identical to the protein’s one. When the symbol is associated with more than one protein, the average values of the frequencies in each category are calculated. In the final st ...
Facilitation of chromatin dynamics by SARs Craig M Hart and Ulrich
... aberrant structures that have a mitotic-like extent of compaction accumulate. Using somatic nuclei, aberrant mitotic nuclei accumulate which contain highly condensed chromatin pushed against the periphery surrounding an ‘empty’ center (Figure 2b). MATH-20 does not inhibit early mitotic events such a ...
... aberrant structures that have a mitotic-like extent of compaction accumulate. Using somatic nuclei, aberrant mitotic nuclei accumulate which contain highly condensed chromatin pushed against the periphery surrounding an ‘empty’ center (Figure 2b). MATH-20 does not inhibit early mitotic events such a ...
beckwith-wiedemann syndrome
... (UPD) which occurs when both copies of chromosome 11 are derived from the same parent. Patients with BWS and paternal UPD have two paternally derived copies of 11p15.5 and no maternal contribution for this region. In these patients, there are methylation defects at both KvDMR1 and H19. ...
... (UPD) which occurs when both copies of chromosome 11 are derived from the same parent. Patients with BWS and paternal UPD have two paternally derived copies of 11p15.5 and no maternal contribution for this region. In these patients, there are methylation defects at both KvDMR1 and H19. ...
Supplementary Materials and Methods (doc 45K)
... Laboratories, Paris, France). All BrdU-labeled cells in the dentate gyrus were counted using a computer image analysis system (Mercator, Explora Nova, La Rochelle, France). Results are presented as the total number of BrdU-immunopositive cells per section. In order to determine the differentiation o ...
... Laboratories, Paris, France). All BrdU-labeled cells in the dentate gyrus were counted using a computer image analysis system (Mercator, Explora Nova, La Rochelle, France). Results are presented as the total number of BrdU-immunopositive cells per section. In order to determine the differentiation o ...
Integrating Functional Genomic Information into the Saccharomyces Genome Database.
... be displayed on SGD’s new locus page. A controlled vocabulary to describe mutant phenotypes is being developed to facilitate quick and accurate searches for genes with similar phenotypes. Three new descriptions will be added to the display on the locus page: function, process and cellular component. ...
... be displayed on SGD’s new locus page. A controlled vocabulary to describe mutant phenotypes is being developed to facilitate quick and accurate searches for genes with similar phenotypes. Three new descriptions will be added to the display on the locus page: function, process and cellular component. ...
Functional analysis of the regulatory region of a zein gene in
... The transcription of zein genes in maize is tissue-specific and developmentally regulated. The 5' regulatory region of many zein genes contains two promoters, P1 and P2, lying approximately 1000 bases apart. The promoter/enhancer activity of various fragments of the two promoter regions of the zein ...
... The transcription of zein genes in maize is tissue-specific and developmentally regulated. The 5' regulatory region of many zein genes contains two promoters, P1 and P2, lying approximately 1000 bases apart. The promoter/enhancer activity of various fragments of the two promoter regions of the zein ...
RNA Processing
... Mutations causing deficiencies in any pathway component can contribute to the development of cancer. ...
... Mutations causing deficiencies in any pathway component can contribute to the development of cancer. ...
Hippocampus duality: memory and novelty detection are subserved
... ABSTRACT: The hippocampus plays a pivotal role both in novelty detection and in long-term memory. The physiological mechanisms underlying these behaviors have yet to be understood in humans. We recorded intracerebral evoked potentials within the hippocampus of epileptic patients (n 5 10) during both ...
... ABSTRACT: The hippocampus plays a pivotal role both in novelty detection and in long-term memory. The physiological mechanisms underlying these behaviors have yet to be understood in humans. We recorded intracerebral evoked potentials within the hippocampus of epileptic patients (n 5 10) during both ...
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... therefore should be endowed with a broad host-range promoter element. The well characterized lac promoter (Plac) of Escherichia coli has been shown to drive expression of cloned genes in a wide variety of Proteobacteria. Such an expression can be constitutive or regulated depending on the absence or ...
... therefore should be endowed with a broad host-range promoter element. The well characterized lac promoter (Plac) of Escherichia coli has been shown to drive expression of cloned genes in a wide variety of Proteobacteria. Such an expression can be constitutive or regulated depending on the absence or ...
Natural selection and the function of genome imprinting:
... factors that enhance or suppress those differences, just as natural selection can act on mutations and also on genetic factors that increase or decrease the rate of mutation. It is at the level of selection of the genetic factors that modify the establishment of epigenetic differences between ...
... factors that enhance or suppress those differences, just as natural selection can act on mutations and also on genetic factors that increase or decrease the rate of mutation. It is at the level of selection of the genetic factors that modify the establishment of epigenetic differences between ...