Bacterial community composition in the rhizosphere of a transgenic
... can vary dramatically depending on the chromosomal insertion site [17] and other genes, e.g. those in proximity to the insertion site, may also be a¡ected. Should such unintended e¡ects have any impact on soil microbial activity, it would likely be seen ¢rst in the rhizosphere. The composition of mi ...
... can vary dramatically depending on the chromosomal insertion site [17] and other genes, e.g. those in proximity to the insertion site, may also be a¡ected. Should such unintended e¡ects have any impact on soil microbial activity, it would likely be seen ¢rst in the rhizosphere. The composition of mi ...
Development and selection of γδ T cells
... Numerous studies have shown that the accessibility of immunoglobulin (Ig) and TCR genes is a critical determinant of rearrangement patterns during development. Accessibility refers to whether chromatin is in a sufficiently open configuration for recombinase to access the recombination signal sequenc ...
... Numerous studies have shown that the accessibility of immunoglobulin (Ig) and TCR genes is a critical determinant of rearrangement patterns during development. Accessibility refers to whether chromatin is in a sufficiently open configuration for recombinase to access the recombination signal sequenc ...
Relationship between expression and methylation of obesity
... The nCounter Analysis System (NanoString Technologies, Seattle, WA, USA) was used to simultaneously assess the absolute expression of 60 genes of interest per subject (n = 80). Candidate genes of interest were selected based on previous findings in our own research (37,39) or because of their puta ...
... The nCounter Analysis System (NanoString Technologies, Seattle, WA, USA) was used to simultaneously assess the absolute expression of 60 genes of interest per subject (n = 80). Candidate genes of interest were selected based on previous findings in our own research (37,39) or because of their puta ...
The MAOA Gene Predicts Credit Card Debt ∗ London School of Economics
... in the propensity to make impulsive, present-oriented decisions is associated with specific cognitive functions. For example, individual differences in valuing immediate and delayed monetary rewards can be traced to separate neural systems (McClure, Laibson, Loewenstein & Cohen 2004) and processes i ...
... in the propensity to make impulsive, present-oriented decisions is associated with specific cognitive functions. For example, individual differences in valuing immediate and delayed monetary rewards can be traced to separate neural systems (McClure, Laibson, Loewenstein & Cohen 2004) and processes i ...
Molecular Mechanisms of Developmental Review
... skip the L1 stage and assume fates associated with L2, followed by normal development to the adult. In contrast, gain-of-function lin-14 alleles lead to a reiteration of L1 cell fates at later developmental stages (Ambros and Horvitz, 1984). These phenotypes, combined with studies of a temperature-s ...
... skip the L1 stage and assume fates associated with L2, followed by normal development to the adult. In contrast, gain-of-function lin-14 alleles lead to a reiteration of L1 cell fates at later developmental stages (Ambros and Horvitz, 1984). These phenotypes, combined with studies of a temperature-s ...
Clinical and genetic patterns ofneurofibromatosis 1 and 2
... The diseases traditionally known as neurofibromatosis have now been formally separated into two types: neurofibromatosis type 1 or NFl (the type described by von Recklinghausen) and neurofibromatosis type 2 or NF2 (a much rarer form).' It is now recognised that although they have overlapping feature ...
... The diseases traditionally known as neurofibromatosis have now been formally separated into two types: neurofibromatosis type 1 or NFl (the type described by von Recklinghausen) and neurofibromatosis type 2 or NF2 (a much rarer form).' It is now recognised that although they have overlapping feature ...
Tissue- and Development-specific Expression of Multiple
... (Clontech, Palo Alto, CA). RNase protection assay was carried out using the RPAII kit (Ambion Inc.). In brief, 100,000 cpm of labeled probe was hybridized against 100 mg total RNA or tRNA overnight at 458C. Samples were digested with RNase A and T1, precipitated, and run on a 5.5% denaturing polyacr ...
... (Clontech, Palo Alto, CA). RNase protection assay was carried out using the RPAII kit (Ambion Inc.). In brief, 100,000 cpm of labeled probe was hybridized against 100 mg total RNA or tRNA overnight at 458C. Samples were digested with RNase A and T1, precipitated, and run on a 5.5% denaturing polyacr ...
gene cloning and identification of the Circumsporozoite protein of
... incubated in 3D11-coated wells for 2 h at room temperature. The wells were washed with PBS-BSA, incubated with 50,000 cpm of 1251I-labeled 3D11 (2 x 107 cpm/,ug) for 2 h at room temperature, washed, and counted in a gamma counter. An inhibition assay (used in Figs. 2B and 3) was performed by mixing ...
... incubated in 3D11-coated wells for 2 h at room temperature. The wells were washed with PBS-BSA, incubated with 50,000 cpm of 1251I-labeled 3D11 (2 x 107 cpm/,ug) for 2 h at room temperature, washed, and counted in a gamma counter. An inhibition assay (used in Figs. 2B and 3) was performed by mixing ...
Unusual mutations in high functioning fragile X males
... are called full fragile X mutations and usually coincide with hypermethylation of the expanded repeat5 6 and of upstream promoter elements.' This results in downregulation of transcription8 and absence of FMR1 protein (FMRP) in cells normally expressing the FMR1 gene."-1' Most patients with full mut ...
... are called full fragile X mutations and usually coincide with hypermethylation of the expanded repeat5 6 and of upstream promoter elements.' This results in downregulation of transcription8 and absence of FMR1 protein (FMRP) in cells normally expressing the FMR1 gene."-1' Most patients with full mut ...
Analytical approaches to RNA profiling data for
... limit the utility of ISH data for detection of cell specific changes in gene expression in complex tissues, we sought to develop an independent method for the quantitative measurement of the specificity of expression of any gene in a given cell type or condition relative to a large number of other c ...
... limit the utility of ISH data for detection of cell specific changes in gene expression in complex tissues, we sought to develop an independent method for the quantitative measurement of the specificity of expression of any gene in a given cell type or condition relative to a large number of other c ...
cis-Regulatory Elements and trans-Acting Factors
... negative regulatory element could bind a specific nuclear protein, present in the submandibular gland, resulting in the inhibition of Reni expression in this tissue.4 Second, the high expression of submandibular Ren2 could be due to the nonfunctionality of the negative regulatory element.4 Recently, ...
... negative regulatory element could bind a specific nuclear protein, present in the submandibular gland, resulting in the inhibition of Reni expression in this tissue.4 Second, the high expression of submandibular Ren2 could be due to the nonfunctionality of the negative regulatory element.4 Recently, ...
The novel genome organization of the insect picorna
... particles indicate they may belong to the picornavirus family, the available molecular data are equivocal. The sequence from the 1600 nucleotides at the 3« end of the CrPV genome has been published (King et al., 1987). It was initially speculated that this region encoded the RNA-dependent RNA polyme ...
... particles indicate they may belong to the picornavirus family, the available molecular data are equivocal. The sequence from the 1600 nucleotides at the 3« end of the CrPV genome has been published (King et al., 1987). It was initially speculated that this region encoded the RNA-dependent RNA polyme ...
X chromosome gene expression in human tissues
... Two genes (TBL1X and SH3BGRL), which should have escaped from inactivation according to the hybrid system, had equal gender expression in our 11 tissues analyzed and none or little expression (V 15%) from the inactive X in fibroblasts. Three genes (GPR143, PIR, and IKBKG), which escaped from inactiv ...
... Two genes (TBL1X and SH3BGRL), which should have escaped from inactivation according to the hybrid system, had equal gender expression in our 11 tissues analyzed and none or little expression (V 15%) from the inactive X in fibroblasts. Three genes (GPR143, PIR, and IKBKG), which escaped from inactiv ...
BPA leaflet testing and inheritance
... porphyria is known, an attack can be treated quickly. Delays in treatment can lead to paralysis and sometimes death. Familial PCT (the type caused by a porphyria gene) is also dominant with the gene affecting about 1 in 5 of those who have it. However, many experts think genetic testing is not usefu ...
... porphyria is known, an attack can be treated quickly. Delays in treatment can lead to paralysis and sometimes death. Familial PCT (the type caused by a porphyria gene) is also dominant with the gene affecting about 1 in 5 of those who have it. However, many experts think genetic testing is not usefu ...
Constant Growth Rate Can Be Supported by Decreasing Energy
... ATP flux, whereas the cells maintain the same growth rate. To test this hypothesis, we used the O2 and CO2 fluxes (Figure 2C) to estimate the rate of ATP production over the range of reported efficiency of oxidative phosphorylation (Hinkle, 2005); see Supplemental Information. The result suggests de ...
... ATP flux, whereas the cells maintain the same growth rate. To test this hypothesis, we used the O2 and CO2 fluxes (Figure 2C) to estimate the rate of ATP production over the range of reported efficiency of oxidative phosphorylation (Hinkle, 2005); see Supplemental Information. The result suggests de ...
Overexpression of miR165 Affects Apical
... meristem. These results demonstrate that overexpression of miR165 causes a severe effect on SAM formation. Another prominent phenotype of the miR165 overpressors was the appearance of red pigments in the narrow cotyledons (Fig. 1D), which was most probably due to an accumulation of anthocyanin. The ...
... meristem. These results demonstrate that overexpression of miR165 causes a severe effect on SAM formation. Another prominent phenotype of the miR165 overpressors was the appearance of red pigments in the narrow cotyledons (Fig. 1D), which was most probably due to an accumulation of anthocyanin. The ...
Ds - e-Acharya
... (LINEs), which are usually repeated many times in the mammalian genome. Both SINEs and LINEs lack LTRs and are thought to transpose through special retrotransposition mechanism that involves transcription of one strand of the retroposon in to RNA. This RNA undergoes conformation change (looping) and ...
... (LINEs), which are usually repeated many times in the mammalian genome. Both SINEs and LINEs lack LTRs and are thought to transpose through special retrotransposition mechanism that involves transcription of one strand of the retroposon in to RNA. This RNA undergoes conformation change (looping) and ...
Introduction to Molecular Diagnostics
... Many of our bodily processes, both normal and abnormal, as well as health or disease states, are driven by the interaction of our genes and the proteins they produce that carry out specific functions within the body. Therefore, the ability to quickly and accurately assess an individual’s health at t ...
... Many of our bodily processes, both normal and abnormal, as well as health or disease states, are driven by the interaction of our genes and the proteins they produce that carry out specific functions within the body. Therefore, the ability to quickly and accurately assess an individual’s health at t ...
Comparative analysis of peanut NBS‐LRR gene clusters suggests
... was also found to have three RGAs (Mt7g088460.1, Mt7g088470.1, Mt7g099490.1) that shared high homology to the peanut RGAs. These two regions (BAC AC169666 and AC149204) are separated by c. 250 kb on Medicago chromosome 7. This finding indicates that disease resistance genes were present in this regi ...
... was also found to have three RGAs (Mt7g088460.1, Mt7g088470.1, Mt7g099490.1) that shared high homology to the peanut RGAs. These two regions (BAC AC169666 and AC149204) are separated by c. 250 kb on Medicago chromosome 7. This finding indicates that disease resistance genes were present in this regi ...
Analysis of genetic structure in Slovak Pinzgau cattle using five
... ARORA et al., 2010). Analysis of milk proteins polymorphism provides useful information to both the breeders and processors of milk. Many research reports have indicated that certain milk protein variants may be associated with milk production, milk composition (ROBITAILLE et al., 2002) and cheese p ...
... ARORA et al., 2010). Analysis of milk proteins polymorphism provides useful information to both the breeders and processors of milk. Many research reports have indicated that certain milk protein variants may be associated with milk production, milk composition (ROBITAILLE et al., 2002) and cheese p ...
Methylation of the Factor IX Gene is the Main Source of Mutations
... A total of 750 mutations in the human coagulation factor IX gene in 806 patients with hemophilia В were analyzed. It was found that 40% of all point mutations occur in 11 "hot spots," which are CG methylation sites where *CG TG or *CG CA substitutions take place. A mechanism is proposed which expl ...
... A total of 750 mutations in the human coagulation factor IX gene in 806 patients with hemophilia В were analyzed. It was found that 40% of all point mutations occur in 11 "hot spots," which are CG methylation sites where *CG TG or *CG CA substitutions take place. A mechanism is proposed which expl ...
Bacterial disease resistance of transgenic hybrid poplar expressing
... Injuries are also caused by bacterial pathogens such as Xanthomonas spp. (Nesme et al. 1994) and Agrobacterium spp. (Ebinuma et al. 1997). Recent progress in both recombinant DNA technologies and plant genetic transformation should facilitate production of transgenic plants with enhanced resistance ...
... Injuries are also caused by bacterial pathogens such as Xanthomonas spp. (Nesme et al. 1994) and Agrobacterium spp. (Ebinuma et al. 1997). Recent progress in both recombinant DNA technologies and plant genetic transformation should facilitate production of transgenic plants with enhanced resistance ...
Anaerobically functioning mitochondria
... The mitochondrion represents a compelling biological model of complex organelle development driven by evolutionary modification of permanently enslaved primordial purple non-sulphur bacteria. As an evolutionary modification, the dynamic nature of the mitochondrion has been observed to exhibit bioche ...
... The mitochondrion represents a compelling biological model of complex organelle development driven by evolutionary modification of permanently enslaved primordial purple non-sulphur bacteria. As an evolutionary modification, the dynamic nature of the mitochondrion has been observed to exhibit bioche ...
Direct Deletion Analysis in Two Duchenne Muscular Dystrophy
... locus STR 7A for the proband (II2), who should have been heterozygous (allels b and c) (Fig. 2a STR 7A). The hemizygosity was suspected because a similar pattern to that obtained for the father was observed in the proband. Also, a reduction in the signal was seen where the maternal allele (2bp light ...
... locus STR 7A for the proband (II2), who should have been heterozygous (allels b and c) (Fig. 2a STR 7A). The hemizygosity was suspected because a similar pattern to that obtained for the father was observed in the proband. Also, a reduction in the signal was seen where the maternal allele (2bp light ...