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Making Transgenic Plants and Animals
Making Transgenic Plants and Animals

... progeny will be brown (which is dominant), indicating fertilization with a germ-line cell (gamete) that ultimately came from a KO-ES cell. Only about 50% of the brown progeny mice, however, will have the KO allele, because the transgenic ES cell that underwent meiosis to produce the germ-line cell w ...
Chapter 2 Evolution, Genetics, and Experience
Chapter 2 Evolution, Genetics, and Experience

... genes (in terms of messenger RNA). There are no genetic effects without this expression. Unlike the operation of DNA, which is active in all cells, gene expression tends to be specific to particular body tissues and to particular phases of development. This class of mechanisms has been called epigen ...
Ch. 4. Modern Genetics
Ch. 4. Modern Genetics

... The insertion of working copies of a gene into the cells of a person with a genetic disorder in an attempt to correct the disorder. ...
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... 7. Repressible operons are involved in anabolic pathways that synthesize substances needed by cells. Eukaryotic Gene Expression - short verison When genes are expressed, the genetic information (base sequence) on DNA is first transcribed (copied) to a molecule of messenger RNA in a process similar t ...
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... • Concentration and quality of a sample of DNA or RNA are measured with a UV spectrophotometer. • Since nitrogenous bases absorb UV light, the more concentrated the DNA solution, the more UV light it will absorb. • A solution containing 50 µg per ml of double strand DNA has an absorbancy (optical de ...
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... E9. One could begin with the assumption that the inactivation of a tumor-suppressor gene would cause cancerous cell growth. If so, one could begin with a normal human line and introduce a transposon. The next step would be to identify cells that have become immortal. This may be possible by identify ...
Biology 3A Exam 3 Study Guide The exam will consist of multiple
Biology 3A Exam 3 Study Guide The exam will consist of multiple

... exons; 5’ cap, poly-A tail, spliceosomes, snRNA, snRNP. What’s the significance of introns and exons. Translation-where does it occur and what is involved: tRNA, anticodon, triplet, amino acid attachment site, amino acids, aminoacyl-tRNA synthetase. what’s inosine? the wobble hypothesis? Where does ...
E1. Due to semiconservative DNA replication, one of the sister
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... E9. One could begin with the assumption that the inactivation of a tumor-suppressor gene would cause cancerous cell growth. If so, one could begin with a normal human line and introduce a transposon. The next step would be to identify cells that have become immortal. This may be possible by identify ...
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AGO1-IP approach to small RNA target discovery in Arabidopsis
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... A drawback of the method is its intrinsic reliance on sufficient VSR expression levels in the tissues of interest. This may partly explain its poor performances in roots (Figure S5), where the 35S promoter is reputed to be only weakly active in several cell layers. Constitutive VSR expression might ...
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... 4. Recombination occurs by _independent assortment and by chromosome crossing over. 5. The DNA sequence at the 5' end of a gene what controls transcription initiation is called the ___promoter_____________. 6. A ___auxotrophic______ strain of Neurospora is one that requires a nutrient in the medium ...
Genetics Review Questions
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... 6. Dominant genes are represented by a capital letter, while a recessive gene is represented by a lowercase letter. 7. PP and pp represent a purebred organism. 8. A hybrid gene pair is also referred to as heterozygous. 9. Offspring inherit one gene from each parent. 10. Pp has genes that are differe ...
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... In conjugation, genetic information is transferred 1. from one cell into the culture medium, where it is taken up by another cell. 2. with the help of a viral go-between. 3. in a bidirectional fashion between two cells. 4. from one bacterium to another. ...
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... The plant cell shown above is in which phase of mitosis? A) anaphase B) interphase C) prophase D) metaphase 28 An animal combines DNA from two parent organisms through sexual reproduction. Organisms that do NOT exchange genetic material must rely on what for new traits? A) meiosis B) mutation C) hem ...
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GORBI: Web application for the prediction of a protein`s functional
GORBI: Web application for the prediction of a protein`s functional

... prokaryotic genomes. The analysis was done via the method of correlating gene occurrence patterns in selected organisms, termed phylogenetic profiling [1]. A machine learning algorithm based on decision trees for Hierarchical Multi-label Classification (HMC) [2] was used, and the annotations are rep ...
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... d. Sample 2 was cut at more restriction sites than was sample 4. e. Sample 4 was cut at more restriction sites than was sample 2. 6. Once a plasmid has incorporated specific genes, such as the gene coding for ampicillin resistance, the plasmid may be cloned by a. inserting it into a virus to generat ...
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Gene Technology Quest – Study Guide KEY What is a genome? A

... The goal of the Human Genome Project is to create maps showing where genes are located on human chromosomes. 17. What results from a vaccination? A vaccination will result in a patient building immunity for the pathogen that has been introduced to their body. The vaccine does not cause the disease, ...
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Therapeutic gene modulation

Therapeutic gene modulation refers to the practice of altering the expression of a gene at one of various stages, with a view to alleviate some form of ailment. It differs from gene therapy in that gene modulation seeks to alter the expression of an endogenous gene (perhaps through the introduction of a gene encoding a novel modulatory protein) whereas gene therapy concerns the introduction of a gene whose product aids the recipient directly.Modulation of gene expression can be mediated at the level of transcription by DNA-binding agents (which may be artificial transcription factors), small molecules, or synthetic oligonucleotides. It may also be mediated post-transcriptionally through RNA interference.
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