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TaqMan-Based Real-Time PCR for Genotyping Common
TaqMan-Based Real-Time PCR for Genotyping Common

... Red and blue lines represent calculations for means of 6 data points for HP5⬘ (CAL Fluor Red 610) and HP2 (CAL Fluor Orange 560), respectively. Eff, efficiency. ...
MOLECULAR BIOLOGY OF FRUIT MATURATION AND
MOLECULAR BIOLOGY OF FRUIT MATURATION AND

... Fruit are an integral part of this endeavor and can be narrowly defined as mature carpels. This definition accurately describes the fruits of tomato, melons, and stone fruits, to name just a few. A more accurate and inclusive definition encompasses extracarpellary tissues that are included at the ma ...
HLA-A, -B
HLA-A, -B

... Genotyping specifies HLA phenotype in individual: A*0101, *0301, Cw*0701,*0702, B*0702,*0801, DRB1*0301,*1501, DQA1*0501,*0102, DQB1*0201,*0602 composed from 2 haplotypes from the parents: A*0101 : Cw*0701 : B*0801 : DRB1*0301 : DQA1*0501 : DQB1*0201 (by serotyping A1-Cw7-B8-DR3-DQ2) A*0301 : Cw*070 ...
Lecture4 - Department of Computer Science
Lecture4 - Department of Computer Science

... • divide query sequence into its constituent overlapping words of length two for proteins or six for nucleic acids • each sequence in the database is also broken up in the same way • Two word lists are compared to find all identical words in both sequences • An initial score is computed based on how ...
Chromosomes Carrying Meiotic Avoidance Loci
Chromosomes Carrying Meiotic Avoidance Loci

... et al., 2004; Conner et al., 2008). By contrast, two independent dominant loci control apomixis in Hieracium praealtum. Genetic markers linked to these loci have been determined using a deletion mapping approach (Catanach et al., 2006). The roles of these two loci and their interactions with the sex ...
Mycoplasma genitalium: a brief review
Mycoplasma genitalium: a brief review

... In 2006, Glass et al. identified 382 of the 482 M. genitalium proteincoding genes as essential.23 A more recent study by Zhang and Lin (2009) showed that M. genitalium needed only 381 essential genes compared to the 642 required by H. influenza.24 This highlights how the very small M. genitalium is ...
Systematic Studies on the Family Cucurbitaceae
Systematic Studies on the Family Cucurbitaceae

... dotted (24). In addition, seeds may have lighter or darker margins (ring), or may be covered by an additional layer of fleshy pericarp in Egusi cultivars, as controlled by the eg gene (Fig. 1) (12, 13). The genes r, t, and w determine seed color. Black is given by triple dominant; mottled is homozyg ...
O A RIGINAL RTICLE
O A RIGINAL RTICLE

... Potato as one of the world’s main tuber crops of the solanaceae family plays an important role in the economics scene. Since one of the major reasons for yield decreasing in the world are abiotic stresses. The aim of this research was producing transgenic potatoes with more tolerance to salinity str ...
TIB_34 - Stored Product Insect and Engineering Research
TIB_34 - Stored Product Insect and Engineering Research

... The question of whether parents should be selected and tested in the same environment in which their progeny are to perform is a problem which has received considerable attention. The fact that the phenotype of an organism is the product of both its genotype and its environment is widely accepted by ...
Are sperm-binding proteins among two closely related frog species
Are sperm-binding proteins among two closely related frog species

... Interestingly, species-specificity in fish seems to be due to the release of peptide chemoattractants (analogous to smell) by the egg. In order for amphibian and mammalian sperm to penetrate through the extracellular coat, sperm exocytotically release the contents of a large, specialized secretory v ...
Document
Document

... alternative polyadenylation a second mRNA of ∼ 2.1 kb [34, 45]. This short mRNA is produced at significant level and may be differently regulated compared to the long mRNA [34]. No 2.1 kb mRNA has been detected in cattle. Whether the deer transcript shows alternative polyadenylation is not known. The ...
MSc Seminar: Donald Dunbar
MSc Seminar: Donald Dunbar

... Models experiments well, and allows replication • chips, samples, treatments, settings, comparisons ...
Control of DNA excision efficiency in Paramecium
Control of DNA excision efficiency in Paramecium

... micronuclei undergo meiosis, whereas the macronuclei degenerate. The fusion of two gametic nuclei produces a zygotic nucleus. This nucleus divides twice and the daughter nuclei then differentiate into a micronucleus or a macronucleus. In the second case, the whole genome is processed through chromos ...
Evidence that MEK1 positively promotes
Evidence that MEK1 positively promotes

... but this is inhibited during meiosis unless the activity of Rad51 is experimentally increased. Further information on how the bias of repair is created comes from the study of a complex containing the proteins Mek1 (also known as Mre4), Hop1 and Red1. This complex is required for a diverse range of ...
Insulin-like growth factor (IGF) signalling is required for early dorso
Insulin-like growth factor (IGF) signalling is required for early dorso

... that are critical for normal neural development and for notochord and somite formation. We initially examined expression of Pax6.2, Six3.1, Rx3 and Otx2 in 10 hpf embryos. At 10 hpf, Pax6.2 expression is normally detected in the forebrain (eye field and lens placodes) and along the midline region (N ...
Charles K. Wairuri , Jacquie E. van der Waals
Charles K. Wairuri , Jacquie E. van der Waals

... pili (Fig. 1B). As indicated earlier, these include a relatively short mature pilin (44 aa) and a ...
Vernalization Gene Architecture as a Predictor of Growth Habit in
Vernalization Gene Architecture as a Predictor of Growth Habit in

... facultative forms can be planted in the fall or spring. Growth habit is important because it determines the range of adaptation. Winter and fall-sown facultative varieties generally yield more than spring-planted forms, provided that they have sufficient winter hardiness. However, a lack of winter ...
Molecular and genetic characterization of genes involved in maize
Molecular and genetic characterization of genes involved in maize

... might be expected, are ubiquitous throughout the plant kingdom (Post-Beittenmiller, 1996). Thus, a great potential exists to introduce plant varieties with superior traits such as increased drought tolerance or pathogen resistance by modifying cuticular waxes. However, before this potential can be r ...
Finding New Clock Components: Past and Future
Finding New Clock Components: Past and Future

... mutations that alter period length by more than 15% (3-4 h) or lead to a complete loss of circadian rhythms. When multiple alleles are available, period-shortening and -lengthening mutations are observed, as well as loss-of-rhythm mutants. Ironically, per met all of these criteria from the beginning ...
SVPaper050711
SVPaper050711

... than previously recognized (Fig. 1a) and consists of a greater variety of molecular structures (Fig. 1b&1c). To explain why we found more, we start by describing how we went about finding SVs. We combined visual inspection of short-read sequencing data with molecular validation to improve automated ...
Modifying effects of phenotypic plasticity on interactions among
Modifying effects of phenotypic plasticity on interactions among

... once induced by an environmental cue becomes canalized, so that it is expressed even when the environmental cue is removed (reviewed by West-Eberhard, 2003; Crispo, 2007). Plasticity could be lost due to neutral processes (i.e. drift) if populations are located in homogeneous environments (i.e. plas ...
Could distal MSH2 upstream deletions cause HNPCC?
Could distal MSH2 upstream deletions cause HNPCC?

... transcriptional start and are unlikely to affect transcription factor binding sites. Farré et al., (2007) Genome Biology 8:R140. ...
The evolution of meiotic sex and its alternatives
The evolution of meiotic sex and its alternatives

... Figure 1. Processes during meiotic prophase I. Proteins in Saccharomyces cerevisiae (after [12 – 15]) involved in each phase are shown inside the figure. Most cases are resolved without recombination (exchange of flanking regions, see red versus blue arrows). (Online version in colour.) natural sele ...
Specific PCR Primers for the Identification of Salmonella enterica
Specific PCR Primers for the Identification of Salmonella enterica

... Salmonella is one of the most common pathogens and a major cause of foodborne diseases in human worldwide (1,2,3). Contaminated poultry products have been identified as the principal sources of Salmonella leading to foodborne illness in human(4.5). The most common serotypes of Salmonella isolated fr ...
lecture
lecture

... mouse genetics 1. Inbreeding as a tool for making genetically uniform strains of mice that are homozygous for every allele in the genome. 2. Brother-sister matings – makes 12.5% of all loci in the genome homozygous (Clarence Little). After 40 generations of brother-sister mating, >99.98% of genome i ...
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Therapeutic gene modulation

Therapeutic gene modulation refers to the practice of altering the expression of a gene at one of various stages, with a view to alleviate some form of ailment. It differs from gene therapy in that gene modulation seeks to alter the expression of an endogenous gene (perhaps through the introduction of a gene encoding a novel modulatory protein) whereas gene therapy concerns the introduction of a gene whose product aids the recipient directly.Modulation of gene expression can be mediated at the level of transcription by DNA-binding agents (which may be artificial transcription factors), small molecules, or synthetic oligonucleotides. It may also be mediated post-transcriptionally through RNA interference.
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