Ribosome profiling reveals post-transcriptional buffering of divergent
... efficiency. The mode of gene regulatory divergence differs for these processes, as trans-regulatory changes play a greater role in divergent mRNA abundance than in divergent translation efficiency. Strikingly, most genes with aberrant transcript abundance in F1 hybrids (either over- or underexpresse ...
... efficiency. The mode of gene regulatory divergence differs for these processes, as trans-regulatory changes play a greater role in divergent mRNA abundance than in divergent translation efficiency. Strikingly, most genes with aberrant transcript abundance in F1 hybrids (either over- or underexpresse ...
Slide 1
... What is Genetic Engineering? …the branch of biology that uses special procedures and techniques to change an organism’s DNA. One example is removing DNA from one organism and inserting it into another organism. In this image, the gene for insulin has been remove from human DNA and inserted into a ba ...
... What is Genetic Engineering? …the branch of biology that uses special procedures and techniques to change an organism’s DNA. One example is removing DNA from one organism and inserting it into another organism. In this image, the gene for insulin has been remove from human DNA and inserted into a ba ...
Hogart A, Leung KN, Wang NJ, Wu DJ, Driscoll J
... To determine the effect of increased 15q11–13 dosage on gene expression in brain, quantitative RT-PCR was used to measure the levels of 10 transcripts in the critical duplication region between BP2 and BP3 and two non-15q11–13 housekeeping gene controls, GAPDH and ACTB. In addition to age and gender ...
... To determine the effect of increased 15q11–13 dosage on gene expression in brain, quantitative RT-PCR was used to measure the levels of 10 transcripts in the critical duplication region between BP2 and BP3 and two non-15q11–13 housekeeping gene controls, GAPDH and ACTB. In addition to age and gender ...
Ph.D. THESIS Analysis of the chromatin structure of MDR1 gene in
... Effect of HDAC inhibition is different in the drug sensitive and drug resistant cells. TSA sensitivity of MDR1 expression proves the important role of histone acetylation in the transcriptional regulation of MDR1. In drug sensitive cells MDR1 expression increased upon TSA treatment in contrast to MC ...
... Effect of HDAC inhibition is different in the drug sensitive and drug resistant cells. TSA sensitivity of MDR1 expression proves the important role of histone acetylation in the transcriptional regulation of MDR1. In drug sensitive cells MDR1 expression increased upon TSA treatment in contrast to MC ...
Full text
... Despite increasing knowledge about mitosis and the cell cycle, many aspects of cytokinesis remain poorly understood. For example, it is not clear how actin and myosin are recruited to the contractile ring, nor how the contractile ring is attached to the cell membrane. The position of the contractile ...
... Despite increasing knowledge about mitosis and the cell cycle, many aspects of cytokinesis remain poorly understood. For example, it is not clear how actin and myosin are recruited to the contractile ring, nor how the contractile ring is attached to the cell membrane. The position of the contractile ...
Growth medium composition-determined regulatory mechanisms
... 1996). The physiological down-regulation of the Pu promoter of TOL plasmids is a complex process controlled by two or more independent mechanisms (Cases & de Lorenzo, 2000). For example, PtsNmediated carbon source inhibition (Cases et al., 1999) and the control of the activity of σ&% (Cases et al., ...
... 1996). The physiological down-regulation of the Pu promoter of TOL plasmids is a complex process controlled by two or more independent mechanisms (Cases & de Lorenzo, 2000). For example, PtsNmediated carbon source inhibition (Cases et al., 1999) and the control of the activity of σ&% (Cases et al., ...
A Recipe for Traits - Teach Genetics Website
... base is referred to by the first letter of its name: Adenine (A), Cytosine (C), Guanine (G) and Thymine (T). The sequence of these chemical bases encodes a detailed set of instructions for building an organism’s traits. (The human genome contains approximately 3 billion pairs or bases!) • Students ...
... base is referred to by the first letter of its name: Adenine (A), Cytosine (C), Guanine (G) and Thymine (T). The sequence of these chemical bases encodes a detailed set of instructions for building an organism’s traits. (The human genome contains approximately 3 billion pairs or bases!) • Students ...
- Repository of the Academy`s Library
... The final publication is available at link.springer.com/article/10.1007/s00299-012-1367-0 ...
... The final publication is available at link.springer.com/article/10.1007/s00299-012-1367-0 ...
Bioinformatics - Sequences and Computers
... familiar with bioinformatics tools for the analysis of sequences. Language and DNA use sequences to communicate information. The sequence elements in language are letters and punctuation, in DNA they are the nucleotides. As the letters in books contain information that is realized by readers, the se ...
... familiar with bioinformatics tools for the analysis of sequences. Language and DNA use sequences to communicate information. The sequence elements in language are letters and punctuation, in DNA they are the nucleotides. As the letters in books contain information that is realized by readers, the se ...
Organization and dynamics of plant interphase chromosomes
... Because composition and organization of repetitive sequences within plant genomes differ from those of mammals and birds by a nearly homogenous distribution of all types of dispersed repeats along all types of chromosomes, chromosome painting (the use of labeled chromosome-specific DNA sequences to ...
... Because composition and organization of repetitive sequences within plant genomes differ from those of mammals and birds by a nearly homogenous distribution of all types of dispersed repeats along all types of chromosomes, chromosome painting (the use of labeled chromosome-specific DNA sequences to ...
Milestone7
... Human Immunodeficiency Virus (HIV) is a virus with a single-stranded RNA genome that is 9749 nucleotides long. Because RNA replication is highly error prone when compared to DNA replication, the HIV virus is constantly mutating. Many of these nucleotide changes result in non-functional viruses, but ...
... Human Immunodeficiency Virus (HIV) is a virus with a single-stranded RNA genome that is 9749 nucleotides long. Because RNA replication is highly error prone when compared to DNA replication, the HIV virus is constantly mutating. Many of these nucleotide changes result in non-functional viruses, but ...
Cell-Free Protein Synthesis Course
... into proteins for “use” for conducting most processes in a cell and multicellular organisms. Hence a wide range of proteins are essential components of every organism and have to perform many distinct functions, for examples as enzymes in metabolic processes or as regulatory switches in cell signali ...
... into proteins for “use” for conducting most processes in a cell and multicellular organisms. Hence a wide range of proteins are essential components of every organism and have to perform many distinct functions, for examples as enzymes in metabolic processes or as regulatory switches in cell signali ...
Proof corrections should be returned in one communication to Justin
... ends. For DNA-strand transferases with higher ATPase activities such as RecA (30 min1) and UvsX (200 min1), this appears to be sufficient for autonomous turnover from heteroduplex DNA. However, eukaryotic Rad51 proteins have a relatively low ATPase activity (<1 ATP hydrolyzed per minute), and th ...
... ends. For DNA-strand transferases with higher ATPase activities such as RecA (30 min1) and UvsX (200 min1), this appears to be sufficient for autonomous turnover from heteroduplex DNA. However, eukaryotic Rad51 proteins have a relatively low ATPase activity (<1 ATP hydrolyzed per minute), and th ...
The Drosophila Cdc6/18 protein has functions in both early and late
... been reported; however, depletion of the protein in Xenopus extracts causes a block before S phase (Coleman et al., 1996). Dominant negative mutants for mammalian cells (Walker A and B box mutants) cause a stop during the S phase of the cell cycle (Herbig et al., 1999), a result that is consistent w ...
... been reported; however, depletion of the protein in Xenopus extracts causes a block before S phase (Coleman et al., 1996). Dominant negative mutants for mammalian cells (Walker A and B box mutants) cause a stop during the S phase of the cell cycle (Herbig et al., 1999), a result that is consistent w ...
Inheritance of Organelle DNA Sequences in a Citrus–Poncirus
... The apparent segregation of the intergeneric F1 progeny with respect to the P. trifoliata mtDNA configurations suggested that these configurations resulted from influence of the nuclear genome. Nuclear alleles might alter mtDNA organization in the progeny, producing P. trifoliata configurations. Alt ...
... The apparent segregation of the intergeneric F1 progeny with respect to the P. trifoliata mtDNA configurations suggested that these configurations resulted from influence of the nuclear genome. Nuclear alleles might alter mtDNA organization in the progeny, producing P. trifoliata configurations. Alt ...
BIOD19H3 Epigenetics in Health and Disease Professor: Winter 2015
... 19. Vassoler FM, White SL, Schmidt HD, Sadri-Vakili G, Pierce RC Epigenetic inheritance of a cocainresistance phenotype. Nat Neurosci. 2013 Jan;16(1):42-7. doi: 10.1038/nn.3280. Epub 2012 Dec 16. [PMID: 23242310] In a case of sex-linked epigenetic inheritance, paternal cocaine use results in a herit ...
... 19. Vassoler FM, White SL, Schmidt HD, Sadri-Vakili G, Pierce RC Epigenetic inheritance of a cocainresistance phenotype. Nat Neurosci. 2013 Jan;16(1):42-7. doi: 10.1038/nn.3280. Epub 2012 Dec 16. [PMID: 23242310] In a case of sex-linked epigenetic inheritance, paternal cocaine use results in a herit ...
Operons
... ___________________________________________________________________________ ___________________________________________________________________________ The co-repressor is the amino acid tryptophan. Thus, when tryptophan is abundant, the repressor is active and will bind to the Operator, preventing ...
... ___________________________________________________________________________ ___________________________________________________________________________ The co-repressor is the amino acid tryptophan. Thus, when tryptophan is abundant, the repressor is active and will bind to the Operator, preventing ...
Chromosomal theory of inheritance
... that come together in the offspring separate again when the offspring produce gametes 2. The law of dominance: hybrids between two alternate forms of a trait resemble one of the ...
... that come together in the offspring separate again when the offspring produce gametes 2. The law of dominance: hybrids between two alternate forms of a trait resemble one of the ...
New genes with old modus operandi
... Weitao, K. Nordström and S. Dasgupta, in preparation). Plasmid pBR322 was used as a probe for the level of supercoiling and it was confirmed that mukB and seqA alter the general supercoiling potential of the cells. Thus, their effect on nucleoid structure is not necessarily due to direct interaction ...
... Weitao, K. Nordström and S. Dasgupta, in preparation). Plasmid pBR322 was used as a probe for the level of supercoiling and it was confirmed that mukB and seqA alter the general supercoiling potential of the cells. Thus, their effect on nucleoid structure is not necessarily due to direct interaction ...
Name that Gene Project The National Center for Biotechnology
... corresponds to the gene named in that line of text. A) For each of the top three matches, click on the Accession link to the right. This will take you to a page with more information about the sequence. Report in the table on the data sheet the entry that appears next to the heading SOURCE ORGANISM. ...
... corresponds to the gene named in that line of text. A) For each of the top three matches, click on the Accession link to the right. This will take you to a page with more information about the sequence. Report in the table on the data sheet the entry that appears next to the heading SOURCE ORGANISM. ...
Mitochondriontoplastid DNA transfer: it happens
... plastid to the mitochondrion predates the mtDNA-to-ptDNA rpl2 migration. In fact, the close proximity of the mitochondrial rpl2 to a ptDNA-derived sequence might have facilitated its eventual integration into the plastid genome via repair of a double-stranded break by homologous recombination betwee ...
... plastid to the mitochondrion predates the mtDNA-to-ptDNA rpl2 migration. In fact, the close proximity of the mitochondrial rpl2 to a ptDNA-derived sequence might have facilitated its eventual integration into the plastid genome via repair of a double-stranded break by homologous recombination betwee ...
Voiumon Numberi7i983 NucleicAcids Research
... alogy of Tn1721 [2,20] and the near identity of the tet determinants of RP1 and Tn1721. In subsequent discussions results pertaining to the tet genes of RP1 and Tn1721 will be taken to be equally applicable to either determinant. Open reading frames Bennett and Shales [21] used insertional inactivat ...
... alogy of Tn1721 [2,20] and the near identity of the tet determinants of RP1 and Tn1721. In subsequent discussions results pertaining to the tet genes of RP1 and Tn1721 will be taken to be equally applicable to either determinant. Open reading frames Bennett and Shales [21] used insertional inactivat ...
Complete Control Retroviral Inducible Mammalian Expression System
... ligand binding, at which time high-level transcriptional activation occurs (i.e., the heterodimer is converted from a tight repressor to a transactivator). In transient assays and stable cell lines harboring receptor expression plasmids in combination with a plasmid bearing an inducible luciferase e ...
... ligand binding, at which time high-level transcriptional activation occurs (i.e., the heterodimer is converted from a tight repressor to a transactivator). In transient assays and stable cell lines harboring receptor expression plasmids in combination with a plasmid bearing an inducible luciferase e ...
Efficient TALEN-mediated gene targeting of chicken
... locus of the non-targeted allele in the targeted male PGC lines and did not detect any instances of indels in the second chromosomal locus (Table S1). It is possible that we did not detect any NHEJ in the second allele because bi-allelic ablation of DDX4 is lethal to male PGCs. This is unlikely as ...
... locus of the non-targeted allele in the targeted male PGC lines and did not detect any instances of indels in the second chromosomal locus (Table S1). It is possible that we did not detect any NHEJ in the second allele because bi-allelic ablation of DDX4 is lethal to male PGCs. This is unlikely as ...
Genetic Imprinting in Maize Bhavani P1*, Harinikumar K. M1
... (HAT), which acetylates H3K16 to impact chromatin architecture (Neal et al., 2000) while SIRT1 is a deacetylase (HDAC) that removes acetyl groups from H1, H3 and H4 (Yi and Luo, 2010). Phosphorylation of histones at serine and threonine residues and ubiquitylation of lysine residues are associated w ...
... (HAT), which acetylates H3K16 to impact chromatin architecture (Neal et al., 2000) while SIRT1 is a deacetylase (HDAC) that removes acetyl groups from H1, H3 and H4 (Yi and Luo, 2010). Phosphorylation of histones at serine and threonine residues and ubiquitylation of lysine residues are associated w ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.