Analysis of a genomic segment of white spot syndrome virus of
... situ hybridizations were performed on sections of infected and uninfected shrimp tissue (data not shown), using the plasmids with BamHI fragments as probes, to confirm the virus origin of the inserts. The enlarged nuclei of cells of infected tissue were heavily stained, whereas sections of uninfecte ...
... situ hybridizations were performed on sections of infected and uninfected shrimp tissue (data not shown), using the plasmids with BamHI fragments as probes, to confirm the virus origin of the inserts. The enlarged nuclei of cells of infected tissue were heavily stained, whereas sections of uninfecte ...
Automated Constraint-Based Nucleotide Sequence Selection for
... To simplify the design of our computational systems, we have developed a tool for constraint-based selection of nucleotide sequences. This tool incorporates domain knowledge that has proven to be important in our experimental process. However, we have also formulated a framework for systematically s ...
... To simplify the design of our computational systems, we have developed a tool for constraint-based selection of nucleotide sequences. This tool incorporates domain knowledge that has proven to be important in our experimental process. However, we have also formulated a framework for systematically s ...
Cloning of PCR products into TOPO TA vectors
... why plasmids are considered clinically important. Because plasmids are much smaller in size compared to bacterial and yeast chromosomes, they can be isolated separately from chromosomes. Molecular biologists have thus used plasmids to great advantage by adapting them to serve as vectors to carry "fo ...
... why plasmids are considered clinically important. Because plasmids are much smaller in size compared to bacterial and yeast chromosomes, they can be isolated separately from chromosomes. Molecular biologists have thus used plasmids to great advantage by adapting them to serve as vectors to carry "fo ...
mutations
... (2) Therefore, a base substitution in DNA might not result in any amino acid change in a protein c) Missense mutation resulting in the replacement of an amino acid with a similar amino acid (1) A substitution of one hydrophobic amino acid for another hydrophobic amino acid might not disrupt the stru ...
... (2) Therefore, a base substitution in DNA might not result in any amino acid change in a protein c) Missense mutation resulting in the replacement of an amino acid with a similar amino acid (1) A substitution of one hydrophobic amino acid for another hydrophobic amino acid might not disrupt the stru ...
Taster Lab Student Doc PDF
... individuals look different. However, if you only look at the DNA of individuals, you might say that different people actually look the same! The human genome contains approximately 3 billion nucleotides (A, T, C, and G) linked together in a specific order on long DNA molecules called chromosomes. Th ...
... individuals look different. However, if you only look at the DNA of individuals, you might say that different people actually look the same! The human genome contains approximately 3 billion nucleotides (A, T, C, and G) linked together in a specific order on long DNA molecules called chromosomes. Th ...
ACEMBL System:
... protein assemblies play an important role in all these biological phenomena. In addition, other processes, e.g. entry of viruses into human cells, also critically hinge on multiple proteins or protein complexes5. Moreover, various prokaryotic microorganisms, with E.coli being the prototypical workho ...
... protein assemblies play an important role in all these biological phenomena. In addition, other processes, e.g. entry of viruses into human cells, also critically hinge on multiple proteins or protein complexes5. Moreover, various prokaryotic microorganisms, with E.coli being the prototypical workho ...
Personal Genetics: PCR Determination of PTC Tasters
... individuals look different. However, if you only look at the DNA of individuals, you might say that different people actually look the same! The human genome contains approximately 3 billion nucleotides (A, T, C, and G) linked together in a specific order on long DNA molecules called chromosomes. Th ...
... individuals look different. However, if you only look at the DNA of individuals, you might say that different people actually look the same! The human genome contains approximately 3 billion nucleotides (A, T, C, and G) linked together in a specific order on long DNA molecules called chromosomes. Th ...
Against the Central Dogma
... The Disease-Related Human LncProfiler kit contains 83 primer sets for studying human LncRNAs, along with 12 primer sets for internal controls and housekeeping genes. Each primer set has been designed according to several individual peer-reviewed literature references that demonstrate the importance ...
... The Disease-Related Human LncProfiler kit contains 83 primer sets for studying human LncRNAs, along with 12 primer sets for internal controls and housekeeping genes. Each primer set has been designed according to several individual peer-reviewed literature references that demonstrate the importance ...
Unit A: Global Agriculture
... 3. DNA is the instruction for the building blocks of what? (insulin is an example of this) 4. What genetic disease did Patrice suffer from? 5. What “project” was completed to map a human’s DNA? 6. Write in complete sentences something you have learned from this unit and/or video ...
... 3. DNA is the instruction for the building blocks of what? (insulin is an example of this) 4. What genetic disease did Patrice suffer from? 5. What “project” was completed to map a human’s DNA? 6. Write in complete sentences something you have learned from this unit and/or video ...
Gene Section FANCF (Fanconi anemia, complementation group F)
... FANCA and FANCG form a complex in the cytoplasm, through a N-term FANCA (involving the nuclear localization signal) - FANCG interaction; FANCC join the complex; phosphorylation of FANCA would induce its translocation into the nucleus.This FA complex translocates into the nucleus, where FANCE and FAN ...
... FANCA and FANCG form a complex in the cytoplasm, through a N-term FANCA (involving the nuclear localization signal) - FANCG interaction; FANCC join the complex; phosphorylation of FANCA would induce its translocation into the nucleus.This FA complex translocates into the nucleus, where FANCE and FAN ...
An Approximate Approach to DNA Denaturation
... modern biophysics because it is at the basis of life. However, it also is a very difficult problem due to the complex role played by RNA polymerases in the process. It is now well established (Freifelder 1987) t h a t local denaturation of DNA is involved so t h a t it is interesting to investigate ...
... modern biophysics because it is at the basis of life. However, it also is a very difficult problem due to the complex role played by RNA polymerases in the process. It is now well established (Freifelder 1987) t h a t local denaturation of DNA is involved so t h a t it is interesting to investigate ...
Advanced Plant Technology Program Vocabulary
... developed further by adapting to a specific geographic location. The word “race” in landrace refers to taxonomic race. For example, if a group of domestic horses escape and adapt to life in the wild, over time their population could develop into a landrace. ...
... developed further by adapting to a specific geographic location. The word “race” in landrace refers to taxonomic race. For example, if a group of domestic horses escape and adapt to life in the wild, over time their population could develop into a landrace. ...
RNA-based regulation of genes of tryptophan synthesis
... capability was dispensed with when the trp aporepressor, while charged and uncharged tRNATrp determine whether transcription organisms evolved that were capable of will or will not be terminated in the operon’s leader region. A poorly expressed internal provides transcripts producing low levels of t ...
... capability was dispensed with when the trp aporepressor, while charged and uncharged tRNATrp determine whether transcription organisms evolved that were capable of will or will not be terminated in the operon’s leader region. A poorly expressed internal provides transcripts producing low levels of t ...
Biological-Anthropology-2nd-Edition-Stanford-Test-Bank
... Show one of several films on molecular biology. Discuss the importance of the interactions of the various cell organelles. A discussion of the origin of the mitochondrion in the animal cell would be an interesting example of evolutionary process here. Discuss the statement made by Roger Williams tha ...
... Show one of several films on molecular biology. Discuss the importance of the interactions of the various cell organelles. A discussion of the origin of the mitochondrion in the animal cell would be an interesting example of evolutionary process here. Discuss the statement made by Roger Williams tha ...
Transcript Isoform Differences Across Human Tissues Are
... interdependent molecular processes that enable genomes to synthesize multiple transcript isoforms per gene 1 . In mammalian genomes, it has been estimated that at least 70% of genes have multiple polyadenylation sites, more than 50% of genes have alternative transcription start sites and nearly all ...
... interdependent molecular processes that enable genomes to synthesize multiple transcript isoforms per gene 1 . In mammalian genomes, it has been estimated that at least 70% of genes have multiple polyadenylation sites, more than 50% of genes have alternative transcription start sites and nearly all ...
We present here a collection of DNA sequence
... with the same slope as the main line. Deletions and insertions can be detected by a jagged line, and inverted repeats are shown (using a window of one nucleotide) by diagonal lines with negative slopes. The user can also use the PC function keys to select an area from the plot to either replot at hi ...
... with the same slope as the main line. Deletions and insertions can be detected by a jagged line, and inverted repeats are shown (using a window of one nucleotide) by diagonal lines with negative slopes. The user can also use the PC function keys to select an area from the plot to either replot at hi ...
The murine homologue of HIRA, a DiGeorge
... various laboratories. These attempts led to the isolation of DGCR3 and DGCR5. DGCR3 is an open reading frame (ORF) of 260 amino acids (aa) that is interrupted by the translocation breakpoint (21). However, no cDNAs were isolated and expression studies were only informative under low stringency condi ...
... various laboratories. These attempts led to the isolation of DGCR3 and DGCR5. DGCR3 is an open reading frame (ORF) of 260 amino acids (aa) that is interrupted by the translocation breakpoint (21). However, no cDNAs were isolated and expression studies were only informative under low stringency condi ...
P.L. 2015, c.127 Revises Standards Related to Forensic DNA Testing
... are favorable to the defendant, a motion for a new trial based upon newly discovered evidence would be granted; (c) explain whether DNA testing was done at any prior time, whether the defendant objected to providing a biological sample for DNA testing, and whether the defendant objected to the admis ...
... are favorable to the defendant, a motion for a new trial based upon newly discovered evidence would be granted; (c) explain whether DNA testing was done at any prior time, whether the defendant objected to providing a biological sample for DNA testing, and whether the defendant objected to the admis ...
Chelatococcus sambhunathii sp. nov., a moderately thermophilic
... synthetic medium defined by Egli et al. (1988). As strain HT4T was isolated from a hot spring, aerobic growth on reduced sulfur compounds was tested in mineral salts medium supplemented with different inorganic sulfur sources (thiosulfate, tetrathionate or elemental sulfur) at a concentration of 0.1 ...
... synthetic medium defined by Egli et al. (1988). As strain HT4T was isolated from a hot spring, aerobic growth on reduced sulfur compounds was tested in mineral salts medium supplemented with different inorganic sulfur sources (thiosulfate, tetrathionate or elemental sulfur) at a concentration of 0.1 ...
Full Text - Genes | Genomes | Genetics
... 2002), and DNA methylation (Yun et al. 2014), though its exact mechanistic role or binding partners in these diverse functions have not been fully resolved. In Drosophila, upSETe00365/e00365 flies were described to be viable, but with a female fertility defect due to derepression of transposable elem ...
... 2002), and DNA methylation (Yun et al. 2014), though its exact mechanistic role or binding partners in these diverse functions have not been fully resolved. In Drosophila, upSETe00365/e00365 flies were described to be viable, but with a female fertility defect due to derepression of transposable elem ...
Expression of the Mitochondrial ATPase6 Gene and Tfam in Down
... Furthermore, we have shown that decreased Tfam expression may cause nondisjunction because the required energy for spindle assembly has not been supplied. Therefore, our research suggests that mitochondrial dysfunction due to various extrinsic or intrinsic influences can induce aneuploidies such as ...
... Furthermore, we have shown that decreased Tfam expression may cause nondisjunction because the required energy for spindle assembly has not been supplied. Therefore, our research suggests that mitochondrial dysfunction due to various extrinsic or intrinsic influences can induce aneuploidies such as ...
CRISPR: The Last Piece of the Genetic Puzzle
... are being developed to overcome these challenges. Synthetic CRISPR RNA (scrRNA) uses chemically modified nucleotides to work around the delivery problem while also managing to increase cleavage activity and decrease off-site mutations.15 Despite the achievements of new CRISPR/Cas9 systems, their app ...
... are being developed to overcome these challenges. Synthetic CRISPR RNA (scrRNA) uses chemically modified nucleotides to work around the delivery problem while also managing to increase cleavage activity and decrease off-site mutations.15 Despite the achievements of new CRISPR/Cas9 systems, their app ...
Solid Tumour Section Soft Tissue Tumors: Low grade fibromyxoid sarcoma
... detected in brain and heart. A splice variant, lacking exon 2, was found in placenta, spleen and fetal liver. Since northern blot analysis was not performed, the possibility of additional splice variants and the actual size of the normal CREB3L2 transcript is not determined yet. The cDNA clones with ...
... detected in brain and heart. A splice variant, lacking exon 2, was found in placenta, spleen and fetal liver. Since northern blot analysis was not performed, the possibility of additional splice variants and the actual size of the normal CREB3L2 transcript is not determined yet. The cDNA clones with ...
Applications of Genomics
... or a personal, professional, or business interest of a member of the writing panel. Specifically, all members of the writing group are required to complete and submit a Disclosure Questionnaire showing all such relationships that might be perceived as real or potential conflicts of interest. This st ...
... or a personal, professional, or business interest of a member of the writing panel. Specifically, all members of the writing group are required to complete and submit a Disclosure Questionnaire showing all such relationships that might be perceived as real or potential conflicts of interest. This st ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.