Protocol for inserting transgene using Tn7 (6/1/06) This system uses
... introduction of transgene DNA into bacteria by standard bacterial transformation. 1. Clone your favourite gene into multiple cloning site of pGRG25 or pGRG36. These plasmids are identical, except that pGRG25 does not have the SmaI site. Unique sites in pGRG36 are PacI, SmaI, NotI, XhoI, AvrII and As ...
... introduction of transgene DNA into bacteria by standard bacterial transformation. 1. Clone your favourite gene into multiple cloning site of pGRG25 or pGRG36. These plasmids are identical, except that pGRG25 does not have the SmaI site. Unique sites in pGRG36 are PacI, SmaI, NotI, XhoI, AvrII and As ...
Comparisons of Maize pericarp color1 Alleles
... P1-wr (Zhang et al., 2000); partial sequence analysis of these clones indicated that they were derived from p2 (Zhang et al., 2003). The second class hybridizes with both probes 15 and 8B and has the 80-bp sequence in the 59 UTR sequence; thus, it appears to contain the p1 gene. Two overlapping p1-c ...
... P1-wr (Zhang et al., 2000); partial sequence analysis of these clones indicated that they were derived from p2 (Zhang et al., 2003). The second class hybridizes with both probes 15 and 8B and has the 80-bp sequence in the 59 UTR sequence; thus, it appears to contain the p1 gene. Two overlapping p1-c ...
Biology Keystone Review Packet
... Catalysts increase the rate of a reaction without being changed by the reaction Substrates are the reactants on which enzymes (catalysts) work Rate of reaction in both directions is increased by the presence of specific enzymes. ____________ _________ refers to the part of an enzyme that interacts w ...
... Catalysts increase the rate of a reaction without being changed by the reaction Substrates are the reactants on which enzymes (catalysts) work Rate of reaction in both directions is increased by the presence of specific enzymes. ____________ _________ refers to the part of an enzyme that interacts w ...
Lecture#6 - Further regulation of the lac operon
... F factor is an E. coli episome - capable of being either either a free plasmid or integrated into bacterial chromosome ...
... F factor is an E. coli episome - capable of being either either a free plasmid or integrated into bacterial chromosome ...
Escherichia coli
... Figure 14.15. A tryptophan auxotrophic mutant. Two Petri-dish cultures are shown. Both contain minimal medium, which provides just the basic nutritional requirements for bacterial growth (nitrogen, carbon and energy sources, plus some salts). The medium on the left is supplemented with tryptophan b ...
... Figure 14.15. A tryptophan auxotrophic mutant. Two Petri-dish cultures are shown. Both contain minimal medium, which provides just the basic nutritional requirements for bacterial growth (nitrogen, carbon and energy sources, plus some salts). The medium on the left is supplemented with tryptophan b ...
Promoter identification and analysis of key glycosphingolipid
... weanling piglets. Glycosphingolipids are not the primary products of this gene cluster. Rather, this gene cluster encodes specific glycosyltransferases that combine the glycosphingolipid precursor, ceramide, with the appropriate oligosaccharide (Hakomori, 2000). Therefore, the expression of the glyc ...
... weanling piglets. Glycosphingolipids are not the primary products of this gene cluster. Rather, this gene cluster encodes specific glycosyltransferases that combine the glycosphingolipid precursor, ceramide, with the appropriate oligosaccharide (Hakomori, 2000). Therefore, the expression of the glyc ...
DNA Extraction - Utah Agriculture in the Classroom
... sources of DNA, such as grapes, also contain a lot of water. If the blended cell soup is too watery, there won't be enough DNA to see. To fix this, go back to the first step and add less water. The cell soup should be opaque, meaning that you can't see through it. ...
... sources of DNA, such as grapes, also contain a lot of water. If the blended cell soup is too watery, there won't be enough DNA to see. To fix this, go back to the first step and add less water. The cell soup should be opaque, meaning that you can't see through it. ...
The effect of isozymes on metabolic activity analysis
... The activity score represents the expected number of solutions in which a gene is active. For an active reaction associated with several isozymes, we think of each isozyme as having equal probability of being active and catalyzing the reaction. That is, we assume that isozymes are not concurrently a ...
... The activity score represents the expected number of solutions in which a gene is active. For an active reaction associated with several isozymes, we think of each isozyme as having equal probability of being active and catalyzing the reaction. That is, we assume that isozymes are not concurrently a ...
Electrophoretic stretching of DNA molecules using microscale T
... Controlled trapping and stretching of DNA molecules are critical for single molecule genomic and polymer physics studies. The authors present a microfabricated T junction which can trap and stretch single free DNA molecules using electrophoretic forces. The device does not require special end functi ...
... Controlled trapping and stretching of DNA molecules are critical for single molecule genomic and polymer physics studies. The authors present a microfabricated T junction which can trap and stretch single free DNA molecules using electrophoretic forces. The device does not require special end functi ...
File - PHHS Biology
... nucleus with transcription. The DNA sequence on one strand of the molecule is transcribed into a molecule called messenger RNA, or mRNA. The mRNA moves out of the nucleus into the cytoplasm of the cell where ribosomes associate with it. In the second phase of protein synthesis, the mRNA code is tran ...
... nucleus with transcription. The DNA sequence on one strand of the molecule is transcribed into a molecule called messenger RNA, or mRNA. The mRNA moves out of the nucleus into the cytoplasm of the cell where ribosomes associate with it. In the second phase of protein synthesis, the mRNA code is tran ...
Biology End-of-Course Review
... cell where ribosomes associate with it. In the second phase of protein synthesis, the mRNA code is translated into the appropriate amino acid sequence. Amino acids are brought to ribosomes by tRNA molecules. As tRNA molecules align themselves along the mRNA molecule, amino acids attached to the tRNA ...
... cell where ribosomes associate with it. In the second phase of protein synthesis, the mRNA code is translated into the appropriate amino acid sequence. Amino acids are brought to ribosomes by tRNA molecules. As tRNA molecules align themselves along the mRNA molecule, amino acids attached to the tRNA ...
Biohazardous Materials/rDNA Summary Form
... ( i.e. name of the vendor, name of the colleague, etc.): What is the room number where biohazardous material will be used and stored: ________________________________________________________________________________________________________ Statement of Understanding: I understand if the above mention ...
... ( i.e. name of the vendor, name of the colleague, etc.): What is the room number where biohazardous material will be used and stored: ________________________________________________________________________________________________________ Statement of Understanding: I understand if the above mention ...
Ch 18
... Some viruses have envelopes that are not derived from plasma membrane. The envelope of the herpesvirus is derived from the nuclear envelope of the host. These double-stranded DNA viruses reproduce within the cell nucleus using viral and cellular enzymes to replicate and transcribe their DNA. I ...
... Some viruses have envelopes that are not derived from plasma membrane. The envelope of the herpesvirus is derived from the nuclear envelope of the host. These double-stranded DNA viruses reproduce within the cell nucleus using viral and cellular enzymes to replicate and transcribe their DNA. I ...
Biology Keystone Review Packet
... Cellular Respiration breaks down carbohydrates like glucose for use in cellular respiration ...
... Cellular Respiration breaks down carbohydrates like glucose for use in cellular respiration ...
Lab 8 Biotech Bacterial Transformation
... However, biotechnology and human manipulation of DNA raises several ethical, social, and medical issues, such as the safety of genetically modified foods. This biotechnology depends on plasmids, small circles of DNA that were found first in bacteria. Plasmids allow molecular biologists to manipulate ...
... However, biotechnology and human manipulation of DNA raises several ethical, social, and medical issues, such as the safety of genetically modified foods. This biotechnology depends on plasmids, small circles of DNA that were found first in bacteria. Plasmids allow molecular biologists to manipulate ...
14. Eukaryotic diversity II
... • Describe the basic life cycle of unicellular protists, being certain to specify the names and ploidy levels of all cells and the names of all processes in that life cycle. • Describe Ursula Goodenough’s research, and explain its significance for understanding the origins of sex and the evolution ...
... • Describe the basic life cycle of unicellular protists, being certain to specify the names and ploidy levels of all cells and the names of all processes in that life cycle. • Describe Ursula Goodenough’s research, and explain its significance for understanding the origins of sex and the evolution ...
Chapter 17
... Myotonic dystrophy (MD), occurring in about 1 of 8000 individuals, is the most common form of muscular dystrophy in adults. The disease, which is characterized by progressive muscle degeneration, is caused by a dominant mutant gene that contains an expanded CAG repeat region. Wild-type alleles of t ...
... Myotonic dystrophy (MD), occurring in about 1 of 8000 individuals, is the most common form of muscular dystrophy in adults. The disease, which is characterized by progressive muscle degeneration, is caused by a dominant mutant gene that contains an expanded CAG repeat region. Wild-type alleles of t ...
Reviewing Biology: The Living Environment
... information that is expressed as the traits of the organism, a phenomenon called gene expression. A single gene (that is, one set of alleles) may control one or several traits. Alternatively, some traits are determined by more than one gene (that is, by more than one set of alleles). Although all th ...
... information that is expressed as the traits of the organism, a phenomenon called gene expression. A single gene (that is, one set of alleles) may control one or several traits. Alternatively, some traits are determined by more than one gene (that is, by more than one set of alleles). Although all th ...
current micro 40/5 - Bashan Foundation
... One of these enzymes, the so-called uptake hydrogenase, catalyzes only H2-oxidation in vitro. It has been described to be confined mainly [10] or exclusively [30] to heterocysts, where it utilizes the H2 formed in parallel with N2-reduction catalyzed by nitrogenase. This hydrogenase transfers the el ...
... One of these enzymes, the so-called uptake hydrogenase, catalyzes only H2-oxidation in vitro. It has been described to be confined mainly [10] or exclusively [30] to heterocysts, where it utilizes the H2 formed in parallel with N2-reduction catalyzed by nitrogenase. This hydrogenase transfers the el ...
final exam in kje-2004
... Question 2. Sequence alignment (6 points) a) (2p) Explain the terms sequence homology, sequence similarity and sequence identity. In relation to the three terms, does it make a difference if DNA or proteins sequences are compared? Sequence homology: a conclusion about the common ancestry of sequenc ...
... Question 2. Sequence alignment (6 points) a) (2p) Explain the terms sequence homology, sequence similarity and sequence identity. In relation to the three terms, does it make a difference if DNA or proteins sequences are compared? Sequence homology: a conclusion about the common ancestry of sequenc ...
Ch8 Cell Reproduction
... 1. Codes for a trait or protein 2. Thousands of different ones 3. Has many nucleotides ...
... 1. Codes for a trait or protein 2. Thousands of different ones 3. Has many nucleotides ...
Biology Chapter 4.1 textbook
... The mitochondria (singular: mitochondrion) are organelles that are specialized to provide energy for cells by changing sugar called glucose into usable energy. The chemical reactions that occur on the many folds of the inner membrane of a mitochondrion provide energy for the cell. Plant cells have s ...
... The mitochondria (singular: mitochondrion) are organelles that are specialized to provide energy for cells by changing sugar called glucose into usable energy. The chemical reactions that occur on the many folds of the inner membrane of a mitochondrion provide energy for the cell. Plant cells have s ...
synthesis Gene Cluster of Streptomyces clavuligerus
... An important question from the regulation point of view is if the two clusters organization occurs in all actinomycetes and how expression of these clusters is controlled by the ArgR repressor. This information is particularly important in S. clavuligerus, since this actinomycete uses arginine as pr ...
... An important question from the regulation point of view is if the two clusters organization occurs in all actinomycetes and how expression of these clusters is controlled by the ArgR repressor. This information is particularly important in S. clavuligerus, since this actinomycete uses arginine as pr ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.