IJEB 52(1) 73-79
... A common method for analysis of 17 amino acids from various insect species and plant parts was standardized using HPLC-PDA. Prior to hydrolysis, lyophilization of test samples was found indispensible to remove excess moisture, which interferes in hydrolysis and separation of amino acids. After the h ...
... A common method for analysis of 17 amino acids from various insect species and plant parts was standardized using HPLC-PDA. Prior to hydrolysis, lyophilization of test samples was found indispensible to remove excess moisture, which interferes in hydrolysis and separation of amino acids. After the h ...
amino acid mixture
... as this which investigate the inhibitory effect of a single peptide. One reason for the lack of inhibitory effect of glyclylglycine could be that this dipeptide is a relatively weak inhibitor of peptide transport. Indeed, Das & Radhakrishnan (1975) have suggested that glycylglycine does have an unus ...
... as this which investigate the inhibitory effect of a single peptide. One reason for the lack of inhibitory effect of glyclylglycine could be that this dipeptide is a relatively weak inhibitor of peptide transport. Indeed, Das & Radhakrishnan (1975) have suggested that glycylglycine does have an unus ...
Molecular Basis for Chiral Selection in RNA Aminoacylation
... A distinguishing characteristic of the biological system is that the building blocks are composed exclusively of homochiral molecules. Since the discovery of the double helical structure of DNA by Watson and Crick [1], the fundamental mechanisms of molecular biology have been elucidated. Crick named ...
... A distinguishing characteristic of the biological system is that the building blocks are composed exclusively of homochiral molecules. Since the discovery of the double helical structure of DNA by Watson and Crick [1], the fundamental mechanisms of molecular biology have been elucidated. Crick named ...
PDF
... and an ESI ion source device was constructed (LCMS/MS). The MS system and data were operated and analyzed using targetlynx software (waters version 4.1). The instrumental parameters were optimized during the direct infusion of the compounds with 50 % solvent (0.1 % acetic acid in water/acetonitrile ...
... and an ESI ion source device was constructed (LCMS/MS). The MS system and data were operated and analyzed using targetlynx software (waters version 4.1). The instrumental parameters were optimized during the direct infusion of the compounds with 50 % solvent (0.1 % acetic acid in water/acetonitrile ...
+ E A.
... According to D.www.pharmacy123.blogfa.com L. Nelson, M. M. Cox :LEHNINGER. PRINCIPLES OF BIOCHEMISTRY Fifth edition ...
... According to D.www.pharmacy123.blogfa.com L. Nelson, M. M. Cox :LEHNINGER. PRINCIPLES OF BIOCHEMISTRY Fifth edition ...
Chapter 1 Non-Canonical Amino Acids in Protein Engineering
... enhanced editing of leucine from its cognate tRNA.38 The authors suggested that the role of T252 is to prevent leucine from entering the editing site, and that substitution of threonine by the smaller alanine residue accounts for the increased editing observed. Replacement of T252 with a larger amin ...
... enhanced editing of leucine from its cognate tRNA.38 The authors suggested that the role of T252 is to prevent leucine from entering the editing site, and that substitution of threonine by the smaller alanine residue accounts for the increased editing observed. Replacement of T252 with a larger amin ...
Amino Acid Requirements for Formation of the
... that the m-mixtures could be used without risk of specific inhibitory action on growth or enzyme formation. Media. The basal medium contained (g.); KH,PO,, 0 . 5 ; Na,HPO,, 12H,O, 1; NaC1, 1.25; (NH,),SO,, 2; glutamic acid, 5; tap water to 1 1.; adjusted to pH 7 with NaOH and autoclaved for 15 min. ...
... that the m-mixtures could be used without risk of specific inhibitory action on growth or enzyme formation. Media. The basal medium contained (g.); KH,PO,, 0 . 5 ; Na,HPO,, 12H,O, 1; NaC1, 1.25; (NH,),SO,, 2; glutamic acid, 5; tap water to 1 1.; adjusted to pH 7 with NaOH and autoclaved for 15 min. ...
Determination of free amino acids in cheeses from the Czech market
... Diluent, Waters AccQ.Tag Amino Acid Analysing Column (Nova-Pak C 18, 4 µ1, 150 × 3.9 mm), and Waters Amino Acid Hydrolysate Standard (each ampoule contains a 2.5mM mixture of the 17 hydrolysate amino acids with the exception of cystine – 1.25mM – aspartic acid (Asp), serine (Ser), glutamic acid (Glu ...
... Diluent, Waters AccQ.Tag Amino Acid Analysing Column (Nova-Pak C 18, 4 µ1, 150 × 3.9 mm), and Waters Amino Acid Hydrolysate Standard (each ampoule contains a 2.5mM mixture of the 17 hydrolysate amino acids with the exception of cystine – 1.25mM – aspartic acid (Asp), serine (Ser), glutamic acid (Glu ...
Pdf - Text of NPTEL IIT Video Lectures
... of these amino acids are Hydroxyproline, γ-Carboxyglutamate, o-Phosphoserine o-Phosphotyrosine which are not common amino acids. Here the Hydroxiproline is formed when the Pralines is attached with a specific –OH group which is one other amino acid not among the common amino acids. It can be observe ...
... of these amino acids are Hydroxyproline, γ-Carboxyglutamate, o-Phosphoserine o-Phosphotyrosine which are not common amino acids. Here the Hydroxiproline is formed when the Pralines is attached with a specific –OH group which is one other amino acid not among the common amino acids. It can be observe ...
Essential amino acid
... intermediate in which both of the nitrogens that will be part of the urea end product are bonded to the same carbon atom. Then arginine is formed and split by hydrolysis to yield urea, which will be excreted. • The net result of the urea cycle is reaction of ammonium ion with aspartate to give urea ...
... intermediate in which both of the nitrogens that will be part of the urea end product are bonded to the same carbon atom. Then arginine is formed and split by hydrolysis to yield urea, which will be excreted. • The net result of the urea cycle is reaction of ammonium ion with aspartate to give urea ...
circular paper chromatography 95 - Journal of the Indian Institute of
... Some of the amino Qc;ds ~re difficult to separate even by multiple ucve]opmenl technique. For cxample, valine-methionine, glulamic :lcidthreonine arc very difficllll to separate from each olher. In such cases other melilOds of identification mllst be adopted. Methionine, if present, can he identifie ...
... Some of the amino Qc;ds ~re difficult to separate even by multiple ucve]opmenl technique. For cxample, valine-methionine, glulamic :lcidthreonine arc very difficllll to separate from each olher. In such cases other melilOds of identification mllst be adopted. Methionine, if present, can he identifie ...
Abstract-- Lactic acid bacteria are characterized
... Abstract-- Lactic acid bacteria are characterized by numerous nutritional requirements. The influence of amino acids and peptides on the growth of Pediococcus pentosaceus c1 isolated from argentinean wines was determined. Cells were growth in synthetic media and in the same media added with the foll ...
... Abstract-- Lactic acid bacteria are characterized by numerous nutritional requirements. The influence of amino acids and peptides on the growth of Pediococcus pentosaceus c1 isolated from argentinean wines was determined. Cells were growth in synthetic media and in the same media added with the foll ...
Amino acids
... carboxylase, some glutamic acids will be dicarboxylic acids, referred to as γ carboxyglutamic acid, that form tight binding sites for calcium ion. ...
... carboxylase, some glutamic acids will be dicarboxylic acids, referred to as γ carboxyglutamic acid, that form tight binding sites for calcium ion. ...
Amino acid
Amino acids (/əˈmiːnoʊ, ˈæmənoʊ, əˈmaɪnoʊ/) are biologically important organic compounds containing amine (-NH2) and carboxylic acid (-COOH) functional groups, usually along with a side-chain specific to each amino acid. The key elements of an amino acid are carbon, hydrogen, oxygen, and nitrogen, though other elements are found in the side-chains of certain amino acids. About 500 amino acids are known and can be classified in many ways. They can be classified according to the core structural functional groups' locations as alpha- (α-), beta- (β-), gamma- (γ-) or delta- (δ-) amino acids; other categories relate to polarity, pH level, and side-chain group type (aliphatic, acyclic, aromatic, containing hydroxyl or sulfur, etc.). In the form of proteins, amino acids comprise the second-largest component (water is the largest) of human muscles, cells and other tissues. Outside proteins, amino acids perform critical roles in processes such as neurotransmitter transport and biosynthesis.In biochemistry, amino acids having both the amine and the carboxylic acid groups attached to the first (alpha-) carbon atom have particular importance. They are known as 2-, alpha-, or α-amino acids (generic formula H2NCHRCOOH in most cases, where R is an organic substituent known as a ""side-chain""); often the term ""amino acid"" is used to refer specifically to these. They include the 22 proteinogenic (""protein-building"") amino acids, which combine into peptide chains (""polypeptides"") to form the building-blocks of a vast array of proteins. These are all L-stereoisomers (""left-handed"" isomers), although a few D-amino acids (""right-handed"") occur in bacterial envelopes and some antibiotics. Twenty of the proteinogenic amino acids are encoded directly by triplet codons in the genetic code and are known as ""standard"" amino acids. The other three (""non-standard"" or ""non-canonical"") are selenocysteine (present in many noneukaryotes as well as most eukaryotes, but not coded directly by DNA), pyrrolysine (found only in some archea and one bacterium) and N-formylmethionine (which is often the initial amino acid of proteins in bacteria, mitochondria, and chloroplasts). Pyrrolysine and selenocysteine are encoded via variant codons; for example, selenocysteine is encoded by stop codon and SECIS element. Codon–tRNA combinations not found in nature can also be used to ""expand"" the genetic code and create novel proteins known as alloproteins incorporating non-proteinogenic amino acids.Many important proteinogenic and non-proteinogenic amino acids also play critical non-protein roles within the body. For example, in the human brain, glutamate (standard glutamic acid) and gamma-amino-butyric acid (""GABA"", non-standard gamma-amino acid) are, respectively, the main excitatory and inhibitory neurotransmitters; hydroxyproline (a major component of the connective tissue collagen) is synthesised from proline; the standard amino acid glycine is used to synthesise porphyrins used in red blood cells; and the non-standard carnitine is used in lipid transport.Nine proteinogenic amino acids are called ""essential"" for humans because they cannot be created from other compounds by the human body and, so, must be taken in as food. Others may be conditionally essential for certain ages or medical conditions. Essential amino acids may also differ between species.Because of their biological significance, amino acids are important in nutrition and are commonly used in nutritional supplements, fertilizers, and food technology. Industrial uses include the production of drugs, biodegradable plastics, and chiral catalysts.