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Chapter 4: The Chemical Basis of Life
Chapter 4: The Chemical Basis of Life

...  A complete protein contains one or more polypeptide chains and may contain a few other chemical groups  Proteins have numerous roles o Carry out _________________________________________ o Pump molecules into and out of a cell o Aid in ____________________________________ Enzymes  Chemical react ...
doc Midterm with answers
doc Midterm with answers

... It allows hemoglobin binding to oxygen to be controlled by noncompetitive inhibition It ensures that hemoglobin can bind oxygen only weakly It allows hemoglobin to shift between low and high affinities for oxygen ...
Three-Point Binding Model
Three-Point Binding Model

... align three binding sites to give high specificity • One problem with model: – Model is a static representation → “lock & key” ...
The Module Manual of Biochemistry
The Module Manual of Biochemistry

... understand the aspects of enzyme kinetics and inhibition, including Michaelis-Menten model, the different kinds of enzyme inhibition, as well as their kinetics characteristics. To have a basic understanding of the ways about the control of enzyme activities and allosteric enzymes. About DNA Structur ...
syllabus - Wofford
syllabus - Wofford

... Section I – Course Introduction – Amino Acids, Peptides, Proteins – Enzymes In this section, you will need to learn the structures of the amino acids. It isn’t so difficult, because you really only need to remember the R groups. Amino acid structures are important for your understanding protein stru ...
Chapter 14 - Richsingiser.com
Chapter 14 - Richsingiser.com

Glucose (sugar) and Oxygen
Glucose (sugar) and Oxygen

... and sunlight to make glucose (sugar) and oxygen. What does Cellular Respiration use in order to make carbon dioxide, water, and ATP? ...
Master Entrance Exam
Master Entrance Exam

... (A) All enzymes of the cycle are located in the cytoplasm, except succinate dehydrogenase, which is bound to the inner mitochondrial membrane. (B) In the presence of malonate, one would expect succinate to accumulate. (C) Oxaloacetate is used as a substrate but is not consumed in the cycle. (D) Succ ...
Chapter-4 part-2 Energy Metabolism
Chapter-4 part-2 Energy Metabolism

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Amino Acids, Proteins, and Enzymes
Amino Acids, Proteins, and Enzymes

... different from the mostly neutral environment of the cell in order to react. Thus, the active site can be more acidic or basic, or provide opportunities for different types of bonding to occur, depending on what type of side chains are present on the amino acids of the active ...
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... D. Enzymes are specialized proteins that function as catalysts for chemical reactions. E. Examples of those important to humans: 1. Digestive enzymes, collagen, etc. Too many to list them all – they make up 15% of your total body mass! ...
Method of Analysis for Feed Enzymes: Methodological Problems?
Method of Analysis for Feed Enzymes: Methodological Problems?

... To measure these activities several analytical methods are used. The more widely used are the colourimetricand the viscometric methods, which are based on the measurement of the quantity of product released during the enzyme reaction on a defined ...
A1990DV41100002
A1990DV41100002

... The 10- to 50-fold increases in ornithine decarboxylase activity less than an hour after stimulation might have involved activationof preexisting enzyme protein rather than new protein synthesis. To explore this question, Diane pretreated animals with actinomycin D to inhibit RNA synthesis, and with ...
Overview on Reactions with Multi
Overview on Reactions with Multi

... Many multi-enzyme reactions run simultaneously side by side in a microbial cell, which makes them highly complex and efficient molecular machines. Enzymes responsible for catalyzing these reactions enable biological processes in all living cells29 with great efficiency and specificity. By investigat ...
Digestive enzymes of the West African giant land snail, Archachatina
Digestive enzymes of the West African giant land snail, Archachatina

... Maltose, sucrose and cellobiose are natural substrates for the snail and activities against them show expected low Km values, particularly maltose, even though it has a strong, and unexplained, substrate inhibition effect. Lactose, on the other hand, is unlikely to be used by the snail and high Km v ...
Spectrophotometer 2 R
Spectrophotometer 2 R

... Because enzymes are not consumed by the reactions they catalyse, enzyme assays usually follow changes in the concentration of either substrates or products to measure the rate of reaction. There are many methods of measurement. Spectrophotometric assays observe change in the absorbance of light betw ...
INDUCTION OF ß-GALACTOSIDASE IN E.COLI
INDUCTION OF ß-GALACTOSIDASE IN E.COLI

... The structure genes of the lac operon encode the information of three proteins (ß-galactosidase, transacetylase and lactose permease) necessary to be synthesized by the cells utilizing lactose as energy source. ß-galactosidase catalyzes the hydrolysis of lactose into glucose and galactose. Besides t ...
Essential Biochemistry. 3rd Edition Brochure
Essential Biochemistry. 3rd Edition Brochure

... Essential Biochemistry, 3rd Edition is comprised of biology, pre–med and allied health topics and presents a broad, but not overwhelming, base of biochemical coverage that focuses on the chemistry behind the biology. Furthermore, it relates the chemical concepts that scaffold the biology of biochemi ...
The Biochemistry of Movement
The Biochemistry of Movement

... This is a very simplified view of a process that has about 140 separate steps each catalysed by an enzyme and, in some cases, requiring the action of coenzymes. The total process of oxidising one glucose molecule is capable of producing 38 ATP molecules. In addition to carbohydrates, other food type ...
An Introduction to Metabolism
An Introduction to Metabolism

... less energy! ...
Metabolism—chapter 4
Metabolism—chapter 4

... -hydrolysis (the adding in of water) occurs when bonds between molecules break apart -did you notice the two way arrows in the above chemical figure? That means the reaction is able to go both ways. Pretty neat, huh? ...
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... 12. Compounds with a single chemical formula but different forms are called _________________________________. 13. Enzyme action is called the ______________________ _____________ model. 14. The formation of polymers from monomers occurs as a result of _________________________________ reactions, an ...
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... strong affinities for sulfhydral (-SH) groups. • Since many enzymes contain -SH as part of their active sites, any chemical which can react with them acts as an irreversible inhibitor. ...
Zoology – The Chemical Basis of Animal Life
Zoology – The Chemical Basis of Animal Life

Ch 3 Lipids
Ch 3 Lipids

... Chapter 3 Enzyme Activity ...
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Enzyme



Enzymes /ˈɛnzaɪmz/ are macromolecular biological catalysts. Enzymes accelerate, or catalyze, chemical reactions. The molecules at the beginning of the process are called substrates and the enzyme converts these into different molecules, called products. Almost all metabolic processes in the cell need enzymes in order to occur at rates fast enough to sustain life. The set of enzymes made in a cell determines which metabolic pathways occur in that cell. The study of enzymes is called enzymology.Enzymes are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Enzymes' specificity comes from their unique three-dimensional structures.Like all catalysts, enzymes increase the rate of a reaction by lowering its activation energy. Some enzymes can make their conversion of substrate to product occur many millions of times faster. An extreme example is orotidine 5'-phosphate decarboxylase, which allows a reaction that would otherwise take millions of years to occur in milliseconds. Chemically, enzymes are like any catalyst and are not consumed in chemical reactions, nor do they alter the equilibrium of a reaction. Enzymes differ from most other catalysts by being much more specific. Enzyme activity can be affected by other molecules: inhibitors are molecules that decrease enzyme activity, and activators are molecules that increase activity. Many drugs and poisons are enzyme inhibitors. An enzyme's activity decreases markedly outside its optimal temperature and pH.Some enzymes are used commercially, for example, in the synthesis of antibiotics. Some household products use enzymes to speed up chemical reactions: enzymes in biological washing powders break down protein, starch or fat stains on clothes, and enzymes in meat tenderizer break down proteins into smaller molecules, making the meat easier to chew.
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