Inhibition by D-Glutamate of Growth and Glutamate
... brought to 5 ml. L-Glutamic acid (L-glu) and/or ~ - g l uwere added in suitable amounts to the medium before sterilization ; sucrose and L-glutamine were sterilized separately (the last by filtration) and added aseptically to the sterile medium. Strains. Most of the work was done with strain no. I f ...
... brought to 5 ml. L-Glutamic acid (L-glu) and/or ~ - g l uwere added in suitable amounts to the medium before sterilization ; sucrose and L-glutamine were sterilized separately (the last by filtration) and added aseptically to the sterile medium. Strains. Most of the work was done with strain no. I f ...
C483 Exam I 2014 Answer Key
... 2) 6pts What are the major differences between a 310 helix and an alpha helix? Why is glycine likely found so often in a 310 helix? Many differences. Most relevant: 310 helix: 3 residues per turn, 10 atoms per H-bond loop. Alpha helix: 3.6 residues per turn, 13 atoms per H-bond loop. Glycine has no ...
... 2) 6pts What are the major differences between a 310 helix and an alpha helix? Why is glycine likely found so often in a 310 helix? Many differences. Most relevant: 310 helix: 3 residues per turn, 10 atoms per H-bond loop. Alpha helix: 3.6 residues per turn, 13 atoms per H-bond loop. Glycine has no ...
BIOCHEMISTRY AND MOLECULAR BIOLOGY Problem Unit Two
... ordinary chemical reactions. What makes these reactions unique is that they proceed very rapidly at relatively low temperatures (physiological temperature, 37°C or 98.6°F, is low when compared to the chemical reactions used in industrial processes). These low temperature reactions have accelerated r ...
... ordinary chemical reactions. What makes these reactions unique is that they proceed very rapidly at relatively low temperatures (physiological temperature, 37°C or 98.6°F, is low when compared to the chemical reactions used in industrial processes). These low temperature reactions have accelerated r ...
Water and Minerals: The Ocean Within
... which ____________________ results in abnormal iron deposits in the liver and other tissues. ...
... which ____________________ results in abnormal iron deposits in the liver and other tissues. ...
... Choice B: A protein has a native molecular weight of 180 kDa. SDS-PAGE obtained in the presence of β-mercatoethanol (BME) shows three bands of equal intensity, with molecular weight of 10 (αchain), 30(β-chain), and 50(γ-chain) kDa. i) What technique was used to determine the native molecular weight? ...
exam2_2011_key
... Choice B: A protein has a native molecular weight of 180 kDa. SDS-PAGE obtained in the presence of -mercatoethanol (BME) shows three bands of equal intensity, with molecular weight of 10 (chain), 30(-chain), and 50(γ-chain) kDa. i) What technique was used to determine the native molecular weight? ...
... Choice B: A protein has a native molecular weight of 180 kDa. SDS-PAGE obtained in the presence of -mercatoethanol (BME) shows three bands of equal intensity, with molecular weight of 10 (chain), 30(-chain), and 50(γ-chain) kDa. i) What technique was used to determine the native molecular weight? ...
Document
... Proteins – General: • > 50 AA • Linus Pauling – 1954 Nobel Prize α-helix and β-pleated sheet • Fredrick Sanger – 1958 Primary structure of beef insulin ...
... Proteins – General: • > 50 AA • Linus Pauling – 1954 Nobel Prize α-helix and β-pleated sheet • Fredrick Sanger – 1958 Primary structure of beef insulin ...
UNIVERSITY OF CALICUT
... Unit V. Aspects of Organic Chemisty (6h.) Classification of isomerism, hydrogen bonds and weak interactions, oxidation reduction reactions, substitution, addition, elimination, condensation and decarboxylation with one example each. Unit VI. Chemical Equilibrium and Catalysis (10h.) Introduction to ...
... Unit V. Aspects of Organic Chemisty (6h.) Classification of isomerism, hydrogen bonds and weak interactions, oxidation reduction reactions, substitution, addition, elimination, condensation and decarboxylation with one example each. Unit VI. Chemical Equilibrium and Catalysis (10h.) Introduction to ...
Genetic Investigation Technologies
... What are microarrays? • A microarray is a DNA “chip” which holds 1000s of different DNA sequences • Each DNA sequence might represent a different gene • Microarrays are useful for measuring differences in gene expression between two cell types • They can also be used to study chromosomal aberration ...
... What are microarrays? • A microarray is a DNA “chip” which holds 1000s of different DNA sequences • Each DNA sequence might represent a different gene • Microarrays are useful for measuring differences in gene expression between two cell types • They can also be used to study chromosomal aberration ...
The Origin of Life - The University of Texas at Dallas
... At the very core of metabolism—the starting point for the synthetic pathways of all biomolecules—is a relatively simple set of reactions known as the citric acid cycle (also called the tricarboxylic acid cycle or the Krebs cycle). The cycle involves eight molecules, each a carboxylic acid (a molecul ...
... At the very core of metabolism—the starting point for the synthetic pathways of all biomolecules—is a relatively simple set of reactions known as the citric acid cycle (also called the tricarboxylic acid cycle or the Krebs cycle). The cycle involves eight molecules, each a carboxylic acid (a molecul ...
One amino acid makes the difference: the formation of ent
... wide array of important functions. With over 60,000 different structures described at present, terpenoids represent the largest and structurally most diverse group of natural products [1]. This biodiversity arises from only a few prenyl diphosphate precursors. Terpene synthases (TPSs), the key enzym ...
... wide array of important functions. With over 60,000 different structures described at present, terpenoids represent the largest and structurally most diverse group of natural products [1]. This biodiversity arises from only a few prenyl diphosphate precursors. Terpene synthases (TPSs), the key enzym ...
The Origin of Life - Frederick H. Willeboordse
... Alexander I. Oparin The theory dates back to 1924 and hence did not take our modern knowledge of genes into account. It is a theory that starts with ...
... Alexander I. Oparin The theory dates back to 1924 and hence did not take our modern knowledge of genes into account. It is a theory that starts with ...
Metabolism of amino acid
... Deficiency in an enzyme, branched-chain α-keto acid dehydrogenase leads to an accumulation of three branchedchain amino acids and their corresponding branched-chain α-keto acids which are excreted in the urine. There is only one dehydrogenase enzyme for all three amino acids. Mental retardation in t ...
... Deficiency in an enzyme, branched-chain α-keto acid dehydrogenase leads to an accumulation of three branchedchain amino acids and their corresponding branched-chain α-keto acids which are excreted in the urine. There is only one dehydrogenase enzyme for all three amino acids. Mental retardation in t ...
VeriScript™ Reverse Transcriptase
... Tested for contaminating endonucleases, exonucleases, and ribonucleases. Unit definition: One unit is the amount of enzyme activity that incorporates 1 nmole of dTTP into acid insoluble material in 10 minutes at 37°C using poly(rA)-oligo(dT)18 as template-primer. Functional test: Functionally tested ...
... Tested for contaminating endonucleases, exonucleases, and ribonucleases. Unit definition: One unit is the amount of enzyme activity that incorporates 1 nmole of dTTP into acid insoluble material in 10 minutes at 37°C using poly(rA)-oligo(dT)18 as template-primer. Functional test: Functionally tested ...
Cellular Respiration - Cathkin High School
... If glucose is not available then the cell can use other substrates including: Other sugar molecules, e.g. fructose, can be converted to glucose or glycolysis intermediates for use as respiratory substrates. Starch and glycogen are broken down to glucose for use as a respiratory ...
... If glucose is not available then the cell can use other substrates including: Other sugar molecules, e.g. fructose, can be converted to glucose or glycolysis intermediates for use as respiratory substrates. Starch and glycogen are broken down to glucose for use as a respiratory ...
200 µmol /L is far too low a concentration of ammonium to affect
... reaction is readily reversible, and the direction of reaction (towards deamination of glutamate or glutamate formation) depends on the relative concentrations of the various substrates. As the concentration of ammonium rises, so the reaction proceeds in the direction of formation of glutamate from k ...
... reaction is readily reversible, and the direction of reaction (towards deamination of glutamate or glutamate formation) depends on the relative concentrations of the various substrates. As the concentration of ammonium rises, so the reaction proceeds in the direction of formation of glutamate from k ...
macromolecules
... 5.4 Proteins have many structures, resulting in a wide range of functions • Proteins account for more than 50% of the dry weight of most cells. • Humans have tens of thousands of different proteins, each with a specific structure and function. • The most important type of protein may be enzymes. • E ...
... 5.4 Proteins have many structures, resulting in a wide range of functions • Proteins account for more than 50% of the dry weight of most cells. • Humans have tens of thousands of different proteins, each with a specific structure and function. • The most important type of protein may be enzymes. • E ...
Analysis of the glycoside hydrolase family 8 catalytic core in
... produced by species of Bacillus. Chitosanolytic enzymes can be useful in producing low molecular weight chitooligosaccharides which have several applications. In addition, a bifunctional enzyme would be more beneficial than the use of two individual enzymes in the production of chitooligosaccharides ...
... produced by species of Bacillus. Chitosanolytic enzymes can be useful in producing low molecular weight chitooligosaccharides which have several applications. In addition, a bifunctional enzyme would be more beneficial than the use of two individual enzymes in the production of chitooligosaccharides ...
Enzyme
Enzymes /ˈɛnzaɪmz/ are macromolecular biological catalysts. Enzymes accelerate, or catalyze, chemical reactions. The molecules at the beginning of the process are called substrates and the enzyme converts these into different molecules, called products. Almost all metabolic processes in the cell need enzymes in order to occur at rates fast enough to sustain life. The set of enzymes made in a cell determines which metabolic pathways occur in that cell. The study of enzymes is called enzymology.Enzymes are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Enzymes' specificity comes from their unique three-dimensional structures.Like all catalysts, enzymes increase the rate of a reaction by lowering its activation energy. Some enzymes can make their conversion of substrate to product occur many millions of times faster. An extreme example is orotidine 5'-phosphate decarboxylase, which allows a reaction that would otherwise take millions of years to occur in milliseconds. Chemically, enzymes are like any catalyst and are not consumed in chemical reactions, nor do they alter the equilibrium of a reaction. Enzymes differ from most other catalysts by being much more specific. Enzyme activity can be affected by other molecules: inhibitors are molecules that decrease enzyme activity, and activators are molecules that increase activity. Many drugs and poisons are enzyme inhibitors. An enzyme's activity decreases markedly outside its optimal temperature and pH.Some enzymes are used commercially, for example, in the synthesis of antibiotics. Some household products use enzymes to speed up chemical reactions: enzymes in biological washing powders break down protein, starch or fat stains on clothes, and enzymes in meat tenderizer break down proteins into smaller molecules, making the meat easier to chew.