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Advanced Enzymology - Makerere University Courses
Advanced Enzymology - Makerere University Courses

Introduction to Lab Ex. 17: Fermentation of Carbohydrates F
Introduction to Lab Ex. 17: Fermentation of Carbohydrates F

... Introduction to Lab Ex. 17 – Fermentation Of Carbohydrates: F-tubes Carbohydrates are good sources of energy for organisms. These compounds, mostly in the form of sugars, are used by bacteria in a variety of processes. The most efficient metabolic process to harvest the energy from sugars is aerobi ...
Metabolic Acidosis
Metabolic Acidosis

... stay has been complicated by aspiration pneumonia and sepsis, requiring prolonged courses of antibiotics. For the past few days, she has been having high temperatures again, and her stool output has increased dramatically . Her most recent stool samples have tested positive for Clostridium difficile ...
Physical Properties - Winthrop University
Physical Properties - Winthrop University

... •Steric hindrances may prevent Sn2 reactions •The concentration of both reactants affects the rate ...
Chapter 3 – Cellular Energy Metabolism
Chapter 3 – Cellular Energy Metabolism

... The major consumers of ATP in the cell (SMR) Remember that the vast majority of work done by a cell depends upon proteins. If we consider the case of muscle (particularly skeletal or cardiac), the shortening of individual cells occurs via interactions between myosin and actin that consume ATP. Also, ...
Major Metabolic Pathway
Major Metabolic Pathway

... With the advent of genetic engineering, it is possible to remove or add genes to an organism to alter its metabolic functions in a predetermined manner (metabolic engineering) It is important to understand the metabolic pathways in natural organisms either to use them directly or to metabolically en ...
Chapter 2: Major Metabolic Pathway
Chapter 2: Major Metabolic Pathway

... With the advent of genetic engineering, it is possible to remove or add genes to an organism to alter its metabolic functions in a predetermined manner (metabolic engineering) It is important to understand the metabolic pathways in natural organisms either to use them directly or to metabolically en ...
LECT23 Enz1
LECT23 Enz1

... 2. Substrate: The target of the enzyme’s action. The molecule that will undergo chemical change as a result of the enzyme 3. Enzyme activity: A measure of the enzymes catalytic effectiveness as manifested by the rate of the reaction catalyzed. 4. Cofactor: A component that works with the enzyme in e ...
metabolic regulation
metabolic regulation

... It is potentially a finer form of regulation because it should: 1 be fast; 2 produce reversible changes (if the effect of whatever interacts with the enzyme is reversible); 3 allow fine tuning of metabolic flow rates, rather than just switching flow on or off (if changes in enzyme activity can be ma ...
Lipid Metabolism
Lipid Metabolism

... • Formation of α,β alkane • Next round: add another malonyl-CoA ...
Photosynthetic Reactions
Photosynthetic Reactions

... of rubisco to react with several substrates decreases the efficiency of the protein. This potential means that if an O2 is randomly passing through the various membranes in the chloroplast it might encounter a rubisco enzyme and react with it. Each of these examples shows the way that the two cycles ...
COPYRIGHTED MATERIAL
COPYRIGHTED MATERIAL

... rest. However, this does not normally lead to a fall in blood glucose because it is balanced by an equivalent rate of glucose production by the liver, so the net flux is zero. This concept of flux can be applied at the cellular, tissue/organ or whole body level, and can also relate to the conversion ...
Lecture 5 & 6 Metabolism S11 Chpt. 6 for HO
Lecture 5 & 6 Metabolism S11 Chpt. 6 for HO

... Non-competitive inhibition - Inhibitor/substrate act at different sites •Regulation (allosteric) •Enzyme poisons (example: mercury) Competitive inhibition - Inhibitor/substrate act at same site ...
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Media:Reports_on_Circuits - Genomics and Bioinformatics

... • Positive Phenotypic Output Enzyme+ sucrose degradation= survival – More certain cell lives because pathway works ...
Fact Sheet - Advanced Equine Solutions
Fact Sheet - Advanced Equine Solutions

... reactions used by all aerobic organisms to generate energy through the oxidation of acetate derived from carbohydrates, fats and proteins into carbon dioxide and chemical energy in the form of adenosine triphosphate(ATP). In addition, the cycle provides precursors of certain amino acids as well as t ...
PDF - Genome Medicine
PDF - Genome Medicine

... his popular HMMER software package for hidden Markov model-based search and analysis of similar protein sequences. His two main points were that HMMER3 is now nearly as fast as BLAST, and that it can use ‘forward’ scores for sequence similarity and give accurate E-values for them. The speed increase ...
BME435 BIOINFORMATICS
BME435 BIOINFORMATICS

...  Structural and functional analysis od genes and genomes and their corresponding products.  COMPUTATIONAL BIOLOGY encompasses all biological areas that involve computation.  E.g. Mathematical modelling of ecosystems  Population dynamics,  Application of Game theory in behavioral studies. ...
Enzymes
Enzymes

... – Allosteric enzymes don’t follow MichaelisMenton kinetics; rather, most follow a sigmoidal model – Does not bind to active site on E • Changes shape of E which either  of  ability to bind with S ...
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ELEM_CouvC_V1n3 copy

... hypothesis by demonstrating the formation of nitrogencontaining amide bonds, which are critical to life’s biochemistry. What made Keller et al.’s reaction especially interesting is that they relied on a reactive intermediate molecule called a thioacid; but one cannot start with a thioacid because su ...
ABG
ABG

... In chemistry class we learned that pH describes the concentration of hydrogen ions, and that a pH of 7.0 is perfectly neutral The acceptable pH range of our blood is 7.35 – 7.45, which is slightly alkaline The body needs to maintain the pH of its chemical broth within fairly narrow limits for proper ...
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Matabolic Stoichiometry and Energetics in

... with oxygen in a cascade of reactions known as the respiratory chain. The energy released in this oxidation is sufficient to form three molecule of ATP from ADP. ...
Chapter 9. Cellular Respiration Other Metabolites
Chapter 9. Cellular Respiration Other Metabolites

...  Fat generates 2x ATP vs. carbohydrate more C in gram of fat  more O in gram of carbohydrate ...
Lecture 13 Networks and Ontology
Lecture 13 Networks and Ontology

... graph. The lower the level, the more detailed knowledge. Each gene is annotated to the terms, reflecting people’s knowledge about it. ...
Control and Integration of Metabolism
Control and Integration of Metabolism

... ƒ Cells continuously adjust rate of metabolic pathways to ensure adequate energy and building blocks are available to carry out normal cellular functions. ƒ Several mechanisms exist to control metabolism through control of certain key enzymes, which play an essential role in control of metabolism as ...
metabolism and function of carbohydrates
metabolism and function of carbohydrates

... sulphate, dermatan sulphate, chondroitin sulphate), monomers, bonds, properties and significance. Glycosaminoglycans as component of proteoglycans, role of proteoglycans. 5. Oligosaccarides of glycoprotein complexes. Role of glycoproteins (give examples of representatives and their role). Structure ...
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Metabolic network modelling



Metabolic network reconstruction and simulation allows for an in-depth insight into the molecular mechanisms of a particular organism. In particular, these models correlate the genome with molecular physiology. A reconstruction breaks down metabolic pathways (such as glycolysis and the Citric acid cycle) into their respective reactions and enzymes, and analyzes them within the perspective of the entire network. In simplified terms, a reconstruction collects all of the relevant metabolic information of an organism and compiles it in a mathematical model. Validation and analysis of reconstructions can allow identification of key features of metabolism such as growth yield, resource distribution, network robustness, and gene essentiality. This knowledge can then be applied to create novel biotechnology.In general, the process to build a reconstruction is as follows: Draft a reconstruction Refine the model Convert model into a mathematical/computational representation Evaluate and debug model through experimentation↑
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