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Lab #7 Exoenzymes, Differential and Selective Media
Lab #7 Exoenzymes, Differential and Selective Media

... 25oC, the gelatin will be liquid (remember what happens to Jell-O at room temperature). If you cool the liquefied gelatin, it will resolidify. Gelatin hydrolysis has been correlated with pathogenicity of some microorganisms. It is thought that pathogenic bacteria may break down tissue and spread to ...
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... However, oxygen availability only determines the fate of the end product and is not required for the actual process of glycolysis itself. In fact, oxygen availability has been shown to have little to do with which of the two end products, lactate or pyruvate is produced. Hence the terms aerobic mean ...
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... 39. How is the structure of cellulose different from that of amylose? b a. Cellulose has α(14) glysidic bond, but amylose has (14) glysidic bond. b. Cellulose has (14) glysidic bond, but amylose has α(14) glysidic bond. c. Cellulose has no branches, but amylose has brances. d. Cellulose has br ...
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... II. SYNTHESIS AND DEGRADATION A. Dehydration synthesis 1. Monomers are joined to form polymers by the removal or a water molecule (dehydration) a) This results in covalent attachment of the subunits (1) The bond forms when a hydrogen from one monomer is linked to a hydroxyl group from another monome ...
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Citric acid cycle



The citric acid cycle – also known as the tricarboxylic acid (TCA) cycle or the Krebs cycle – is a series of chemical reactions used by all aerobic organisms to generate energy through the oxidation of acetate derived from carbohydrates, fats and proteins into carbon dioxide and chemical energy in the form of adenosine triphosphate (ATP). In addition, the cycle provides precursors of certain amino acids as well as the reducing agent NADH that is used in numerous other biochemical reactions. Its central importance to many biochemical pathways suggests that it was one of the earliest established components of cellular metabolism and may have originated abiogenically.The name of this metabolic pathway is derived from citric acid (a type of tricarboxylic acid) that is consumed and then regenerated by this sequence of reactions to complete the cycle. In addition, the cycle consumes acetate (in the form of acetyl-CoA) and water, reduces NAD+ to NADH, and produces carbon dioxide as a waste byproduct. The NADH generated by the TCA cycle is fed into the oxidative phosphorylation (electron transport) pathway. The net result of these two closely linked pathways is the oxidation of nutrients to produce usable chemical energy in the form of ATP.In eukaryotic cells, the citric acid cycle occurs in the matrix of the mitochondrion. In prokaryotic cells, such as bacteria which lack mitochondria, the TCA reaction sequence is performed in the cytosol with the proton gradient for ATP production being across the cell's surface (plasma membrane) rather than the inner membrane of the mitochondrion.
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