Molecular biology Tools
... Ex. Just getting down into a squat *Degenerative meniscal tears can lead to osteoarthritis* ...
... Ex. Just getting down into a squat *Degenerative meniscal tears can lead to osteoarthritis* ...
SCI Digestion and Bowel Function
... 1. A properly functioning digestive system enables the body to absorb the nutrients it needs to feed cells and provide the body with energy to carry out all its daily tasks and build a healthy, strong body. If this system is not working well and nutrients are not being absorbed properly, it can nega ...
... 1. A properly functioning digestive system enables the body to absorb the nutrients it needs to feed cells and provide the body with energy to carry out all its daily tasks and build a healthy, strong body. If this system is not working well and nutrients are not being absorbed properly, it can nega ...
Organic Molecules
... • The polarity of amino acids play a crucial role in determining the overall 3 dimensional structure of proteins which in turn determines its biological function ...
... • The polarity of amino acids play a crucial role in determining the overall 3 dimensional structure of proteins which in turn determines its biological function ...
Comparison of p53 Structure: Wild type vs. mutant
... molecule (1tup) as ribbon diagram Figure from ConSurf ...
... molecule (1tup) as ribbon diagram Figure from ConSurf ...
1 Biology - Organic Chemistry Notes Name Date Organic Chemistry
... place in order to maintain life. Large organic food molecules such as proteins and starches must initially be broken down (digested to amino acids and simple sugars respectively), in order to enter cells. Once nutrients enter a cell, the cell will use them as building blocks in the synthesis of comp ...
... place in order to maintain life. Large organic food molecules such as proteins and starches must initially be broken down (digested to amino acids and simple sugars respectively), in order to enter cells. Once nutrients enter a cell, the cell will use them as building blocks in the synthesis of comp ...
ion Channels: an examPle of hoW ComPlexity Could evolve
... functioning ion channels are made by combining two copies of channel-A and one copy each of channel-B and channel-C. So while the best-functioning ion channels are more complex than the original ion channel, they are not yet irreducibly complex. At this point it might happen that a mutation in chann ...
... functioning ion channels are made by combining two copies of channel-A and one copy each of channel-B and channel-C. So while the best-functioning ion channels are more complex than the original ion channel, they are not yet irreducibly complex. At this point it might happen that a mutation in chann ...
Organic Molecules
... • The polarity of amino acids play a crucial role in determining the overall 3 dimensional structure of proteins which in turn determines its biological function ...
... • The polarity of amino acids play a crucial role in determining the overall 3 dimensional structure of proteins which in turn determines its biological function ...
Leukaemia Section inv(11)(q13q23) Atlas of Genetics and Cytogenetics
... MLL, including those domains known to be essential for leukemic transformation: the AT-hooks and the DNA methyltransferase domain (DNMT). The Cterminal sequences are derived from the BTBD18 protein, a new fusion partner. The fusion occurred with in the BTB/POZdomain of BTBD18 (Alonso et al., ...
... MLL, including those domains known to be essential for leukemic transformation: the AT-hooks and the DNA methyltransferase domain (DNMT). The Cterminal sequences are derived from the BTBD18 protein, a new fusion partner. The fusion occurred with in the BTB/POZdomain of BTBD18 (Alonso et al., ...
Classroom Cell Communication
... There are 3 phases of signal reception, Reception, Transduction, and Response. A signal ligand (hormone or environmental stimulus) will bind to a receptor protein on the cell membrane. The binding to the receptor protein will initiate a transduction of the outside signal through the cytoplasm via se ...
... There are 3 phases of signal reception, Reception, Transduction, and Response. A signal ligand (hormone or environmental stimulus) will bind to a receptor protein on the cell membrane. The binding to the receptor protein will initiate a transduction of the outside signal through the cytoplasm via se ...
protein synthesis
... determine the amino acid sequence that results from the transcription and translation of the following nucleotide sequence: 5’ TACTCGGCATTGTGA 3’ ...
... determine the amino acid sequence that results from the transcription and translation of the following nucleotide sequence: 5’ TACTCGGCATTGTGA 3’ ...
Supplementary Methods and References
... PCR amplified using the primers Ecorev (5’-ggggtgttgGAATTCgggcgaaaaaccgtctatcaggg-3’) and Clafor (5’-gggaagaggATCGAT gggagaggcggtttgcgtattg-3’), and following EcoRI and ClaI digestion was ligated to a 5.6 Kb fragment of pPCV002. The resulting vector pGAP-Km (7890bp) carries a polylinker with multipl ...
... PCR amplified using the primers Ecorev (5’-ggggtgttgGAATTCgggcgaaaaaccgtctatcaggg-3’) and Clafor (5’-gggaagaggATCGAT gggagaggcggtttgcgtattg-3’), and following EcoRI and ClaI digestion was ligated to a 5.6 Kb fragment of pPCV002. The resulting vector pGAP-Km (7890bp) carries a polylinker with multipl ...
A review of quantitative methods for proteomic studies
... The addition of synthetic internal standards has been used for nearly 40 years for quantitation with mass spectrometry, and is compatible with both LC-ES-MS and MALDI-MS for proteomic analyses. In the most common application to proteomics, peptides are selected, based on uniqueness, stability, sensi ...
... The addition of synthetic internal standards has been used for nearly 40 years for quantitation with mass spectrometry, and is compatible with both LC-ES-MS and MALDI-MS for proteomic analyses. In the most common application to proteomics, peptides are selected, based on uniqueness, stability, sensi ...
Organelles at Work
... distilled water to cylinder 2 and 10 drops of distilled water to cylinder 3. ...
... distilled water to cylinder 2 and 10 drops of distilled water to cylinder 3. ...
Heat shock Proteins (HSPs)
... response to various biological stresses, including heat, high pressures, and toxic compounds. It is also one of the most abundant cellular proteins found under nonstress conditions ...
... response to various biological stresses, including heat, high pressures, and toxic compounds. It is also one of the most abundant cellular proteins found under nonstress conditions ...
Issues in predicting protein function from sequence
... contain protein kinase and src homology 3 (SH3) domains, but in different linear orders. Moreover, the bacterial SH3 domain (SH3b) is predicted to be extracellular, whereas MST is predicted to be cytoplasmic. (b) Yeast (Saccharomyces cerevisiae) Bem1p and human p47 phox contain SH3 and PX domains bu ...
... contain protein kinase and src homology 3 (SH3) domains, but in different linear orders. Moreover, the bacterial SH3 domain (SH3b) is predicted to be extracellular, whereas MST is predicted to be cytoplasmic. (b) Yeast (Saccharomyces cerevisiae) Bem1p and human p47 phox contain SH3 and PX domains bu ...
Problem Set 4-key
... In order to figure out the number of possible different mRNAs, you simply multiply these numbers (2x1x2x2x6x6x4), and you get 1152 possible RNA sequences that would code for “AMHERST”... now, just when you are feeling pretty good about your odds of having found a correct RNA sequence to encode AM ...
... In order to figure out the number of possible different mRNAs, you simply multiply these numbers (2x1x2x2x6x6x4), and you get 1152 possible RNA sequences that would code for “AMHERST”... now, just when you are feeling pretty good about your odds of having found a correct RNA sequence to encode AM ...
Poster - Protein Information Resource
... have accurate, consistent, and rich annotation of protein sequence and function. Relevant to this goal are the literature-based curation and development and adoption of ontologies and controlled vocabularies. • Literature-Based Curation – Extract Reliable Information from Literature • Protein proper ...
... have accurate, consistent, and rich annotation of protein sequence and function. Relevant to this goal are the literature-based curation and development and adoption of ontologies and controlled vocabularies. • Literature-Based Curation – Extract Reliable Information from Literature • Protein proper ...
lecture 5
... evolutionary origin: the structural similarities could arise just from the physics and chemistry of proteins favoring certain packing arrangements and chain topologies. Class: secondary structure content and organization Murzin et al. J. Mol. Biol. 247, 536-540, 1995 ...
... evolutionary origin: the structural similarities could arise just from the physics and chemistry of proteins favoring certain packing arrangements and chain topologies. Class: secondary structure content and organization Murzin et al. J. Mol. Biol. 247, 536-540, 1995 ...
Chapter 3
... Protein structure (continued) -tertiary structure – folded shape of the polypeptide chain -quaternary structure – interactions between multiple polypeptide subunits Protein folding is aided by chaperone proteins. ...
... Protein structure (continued) -tertiary structure – folded shape of the polypeptide chain -quaternary structure – interactions between multiple polypeptide subunits Protein folding is aided by chaperone proteins. ...
Unit 1 exam Review
... 7. RESPONDS TO STIMULI: Organisms must respond to stimuli appropriately in order to stay alive Stimulus = anything in an organism’s external or internal environment that causes a reaction or response Response = a reaction to a stimulus Ex) if a shark smells blood in the water, it will respond by mo ...
... 7. RESPONDS TO STIMULI: Organisms must respond to stimuli appropriately in order to stay alive Stimulus = anything in an organism’s external or internal environment that causes a reaction or response Response = a reaction to a stimulus Ex) if a shark smells blood in the water, it will respond by mo ...
fulltext
... to an extra-cellular part of G-protein coupled receptors and, then, the pheromone-lipocalin complexes can pass the information from chemosensory neurons to CNS via long axons. Major Urinary Proteins (Mups) are the most studied group of lipocalins associated with chemical communication. In Mus muscul ...
... to an extra-cellular part of G-protein coupled receptors and, then, the pheromone-lipocalin complexes can pass the information from chemosensory neurons to CNS via long axons. Major Urinary Proteins (Mups) are the most studied group of lipocalins associated with chemical communication. In Mus muscul ...
Function
... smaller molecules so that they can be used by the cell. They also break down organelles that have outlived their usefulness (remove junk that accumulates in the cell). Many serious diseases can be traced to lysosomes that fail to function properly. ...
... smaller molecules so that they can be used by the cell. They also break down organelles that have outlived their usefulness (remove junk that accumulates in the cell). Many serious diseases can be traced to lysosomes that fail to function properly. ...
Document
... amino acid similarity to the PAX family of transcription factors; however, there is no obvious similarity to any one specific class of PAX protein, say like PAX6; hence, you have discovered a new subfamily that you have named KAX for kidney specific Pax. Your next experiment is to isolate homologous ...
... amino acid similarity to the PAX family of transcription factors; however, there is no obvious similarity to any one specific class of PAX protein, say like PAX6; hence, you have discovered a new subfamily that you have named KAX for kidney specific Pax. Your next experiment is to isolate homologous ...
Biology 2.3 2.4 - Sleeping Dog Studios
... Lipids are nonpolar molecules that are not soluble or mostly insoluble in water. They will not dissolve into water! ...
... Lipids are nonpolar molecules that are not soluble or mostly insoluble in water. They will not dissolve into water! ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.