Plant mitochondria contain the protein translocase subunits TatB
... Journal of Cell Science • Advance article ...
... Journal of Cell Science • Advance article ...
mic.sgmjournals.org
... for the adaptation of Y. pestis to its in vivo life stages. Thirteen proteins that appear to be part of an intact type VI secretion system (T6SS) were identified in membrane fractions of stationaryphase cells grown at 26 6C, but not at 37 6C. The corresponding genes are clustered in the Y. pestis KI ...
... for the adaptation of Y. pestis to its in vivo life stages. Thirteen proteins that appear to be part of an intact type VI secretion system (T6SS) were identified in membrane fractions of stationaryphase cells grown at 26 6C, but not at 37 6C. The corresponding genes are clustered in the Y. pestis KI ...
A Dictyostelium Mutant with Reduced Lysozyme Levels
... dilution into multiwell plates. To identify clones that carry a disrupted lysozyme gene, we performed PCR on preparations of genomic DNA (29) using primers (5⬘-GTGGTGCTCTCTATTTCACAGC-3⬘ and 5⬘-CTTGTTCAACCCACATGGCTGG-3⬘) that flank 85 bp of the coding sequence containing two introns of different leng ...
... dilution into multiwell plates. To identify clones that carry a disrupted lysozyme gene, we performed PCR on preparations of genomic DNA (29) using primers (5⬘-GTGGTGCTCTCTATTTCACAGC-3⬘ and 5⬘-CTTGTTCAACCCACATGGCTGG-3⬘) that flank 85 bp of the coding sequence containing two introns of different leng ...
Protein kinase signaling networks in plant innate immunity
... future research will reveal more Ca2+-regulated PKs involved in plant immunity. These PKs may be activated in different cellular locales by localized Ca2+ signals to regulate specific responses or converge to common targets for a fine-tuned regulation of downstream responses. Only few substrates of ...
... future research will reveal more Ca2+-regulated PKs involved in plant immunity. These PKs may be activated in different cellular locales by localized Ca2+ signals to regulate specific responses or converge to common targets for a fine-tuned regulation of downstream responses. Only few substrates of ...
Plant mitochondria contain the protein translocase subunits TatB
... Journal of Cell Science • Advance article ...
... Journal of Cell Science • Advance article ...
An in silico analysis of the mitochondrial protein import apparatus of
... imported into these organelles. Notably, the proteomes of both mitochondria and chloroplasts are derived from a variety of sources and are not simply a subset of the proteins derived from the ancestral endosymbiont [9]. In the most extreme cases, it is thought that all genes that were present in the ...
... imported into these organelles. Notably, the proteomes of both mitochondria and chloroplasts are derived from a variety of sources and are not simply a subset of the proteins derived from the ancestral endosymbiont [9]. In the most extreme cases, it is thought that all genes that were present in the ...
Infectious Salmon Anaemia Virus (ISAV) RNA Binding Protein
... salmon and in cell lines derived from Atlantic salmon [7–9]. The type I IFNs are produced in response to viral nucleic acids detected by pattern recognition receptors (PRRs) and lead to transcription of interferon stimulated genes (ISGs) [10]. ISG15 is an ubiquitin-like protein modifier that is stro ...
... salmon and in cell lines derived from Atlantic salmon [7–9]. The type I IFNs are produced in response to viral nucleic acids detected by pattern recognition receptors (PRRs) and lead to transcription of interferon stimulated genes (ISGs) [10]. ISG15 is an ubiquitin-like protein modifier that is stro ...
Anti-PYRUVATE KINASE (Rabbit Muscle) (GOAT) Antibody
... This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American o ...
... This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American o ...
Disparate proteins use similar architectures to damage membranes
... are hydrophobic, the transmembrane region can feature alternate runs of as few as four hydrophobic amino acids. Such a structure can be easily formed from small regions of the soluble protein, and the transmembrane regions are often insignificant in the structure of the soluble monomer. Helical prot ...
... are hydrophobic, the transmembrane region can feature alternate runs of as few as four hydrophobic amino acids. Such a structure can be easily formed from small regions of the soluble protein, and the transmembrane regions are often insignificant in the structure of the soluble monomer. Helical prot ...
Sample pages 1 PDF
... mechanisms, as the vesicles and endosomes move on actin filaments or microtubules. Selection and movement of the protein cargo to be trafficked to and from the plasma membrane depends to a great extent on signature organellar targeting motifs within the proteins themselves as well as interactions wi ...
... mechanisms, as the vesicles and endosomes move on actin filaments or microtubules. Selection and movement of the protein cargo to be trafficked to and from the plasma membrane depends to a great extent on signature organellar targeting motifs within the proteins themselves as well as interactions wi ...
Systematic Analysis of Arabidopsis Organelles
... is a time-consuming and costly endeavor. Computational methods could help to make this process more rapid by providing some clues about a protein’s potential location (Claros and Vincens, 1996; Cedano et al., 1997; Nakai and Horton, 1999; Emanuelsson et al., 2000; Chou, 2001; Hua and Sun, 2001; Chou ...
... is a time-consuming and costly endeavor. Computational methods could help to make this process more rapid by providing some clues about a protein’s potential location (Claros and Vincens, 1996; Cedano et al., 1997; Nakai and Horton, 1999; Emanuelsson et al., 2000; Chou, 2001; Hua and Sun, 2001; Chou ...
Carbohydrates - Overview
... in soybean-based feed because they lack the enzyme α-galactosidase. To prevent dietary problems associated with the inability to digest these oligosaccharides, soybean feed is pre-treated with a commercially-produced αgalactosidase enzyme. This same α-galactosidase enzyme preparation (isolated from ...
... in soybean-based feed because they lack the enzyme α-galactosidase. To prevent dietary problems associated with the inability to digest these oligosaccharides, soybean feed is pre-treated with a commercially-produced αgalactosidase enzyme. This same α-galactosidase enzyme preparation (isolated from ...
Quantitative Host Cell Protein Analysis Using Two
... content provided by ELISA analysis is currently acceptable for lot-release, it does not provide any information on the profile or properties of HCPs present which may be used for process design improvement. Hence methods that facilitate identification of individual HCPs have gained prominence in recen ...
... content provided by ELISA analysis is currently acceptable for lot-release, it does not provide any information on the profile or properties of HCPs present which may be used for process design improvement. Hence methods that facilitate identification of individual HCPs have gained prominence in recen ...
Avirulence proteins from haustoria
... that the first Avr genes from haustoria-forming pathogens were cloned and characterized. Among the first of these was AvrL567, from the flax rust fungus Melampsora lini (Dodds et al., 2004). This gene was identified through the use of cDNA subtraction to generate a library enriched for rust genes expres ...
... that the first Avr genes from haustoria-forming pathogens were cloned and characterized. Among the first of these was AvrL567, from the flax rust fungus Melampsora lini (Dodds et al., 2004). This gene was identified through the use of cDNA subtraction to generate a library enriched for rust genes expres ...
ID helix-loop-helix proteins - Journal of Cell Science
... Finally, Kondo and Raff (Kondo and Raff, 2000) have recently implicated ID4, which has been less well studied than its relatives, in the ‘molecular clock’ mechanism controlling the timing of oligodendrocyte differentiation. The above studies clearly illustrate the complex role played by ID proteins ...
... Finally, Kondo and Raff (Kondo and Raff, 2000) have recently implicated ID4, which has been less well studied than its relatives, in the ‘molecular clock’ mechanism controlling the timing of oligodendrocyte differentiation. The above studies clearly illustrate the complex role played by ID proteins ...
Selenium incorporation using recombinant techniques
... decades later, this trick was exploited in the development of powerful phasing techniques to solve the phase problem in macromolecular crystallography (Hendrickson et al., 1990). Hendrickson first reported the possibility of using selenomethionine (SeMet) as a phasing tool in 1990 and the ease of in ...
... decades later, this trick was exploited in the development of powerful phasing techniques to solve the phase problem in macromolecular crystallography (Hendrickson et al., 1990). Hendrickson first reported the possibility of using selenomethionine (SeMet) as a phasing tool in 1990 and the ease of in ...
Chapter-Translation (Prokaryotes)
... The phenomenon of interpretation of the genetic information contained in the mRNA and converting it into a sequence of amino acids joined by peptide linkages to form a protein molecule, has always intrigued molecular biologists. This process of translation is highly conserved among all organisms and ...
... The phenomenon of interpretation of the genetic information contained in the mRNA and converting it into a sequence of amino acids joined by peptide linkages to form a protein molecule, has always intrigued molecular biologists. This process of translation is highly conserved among all organisms and ...
Based Method for Identification of Horizontal Gene Transfer in Bacteria
... Horizontal gene transfer (HGT) or lateral gene transfer is the passing of genetic material from one organism to another, other than by descent in which genetic information travels through the generations as the cell divides. In nature, gene transfer occurs between two same species or closely related ...
... Horizontal gene transfer (HGT) or lateral gene transfer is the passing of genetic material from one organism to another, other than by descent in which genetic information travels through the generations as the cell divides. In nature, gene transfer occurs between two same species or closely related ...
Lecture 7: Signal Transduction
... – Ri = the inactivated receptor (cannot be activated) – Rs = the susceptible receptor (can be activated) – Ra = the activated receptor (bound to the ligand) ...
... – Ri = the inactivated receptor (cannot be activated) – Rs = the susceptible receptor (can be activated) – Ra = the activated receptor (bound to the ligand) ...
Broder et al Curr biol 98
... Grb2 fused to either myristoylation (M) or myristoylated-defective (M*) sequence [8]. PLCγ2 amino acids 405–1252, p85 amino acids 2–84 and mGAP amino acids 262–345 were fused to the v-Src myristoylation signal. The Rac1 constructs used were: wild-type Rac1 (Rac1 Wt); constitutively active Rac1 Q61L ...
... Grb2 fused to either myristoylation (M) or myristoylated-defective (M*) sequence [8]. PLCγ2 amino acids 405–1252, p85 amino acids 2–84 and mGAP amino acids 262–345 were fused to the v-Src myristoylation signal. The Rac1 constructs used were: wild-type Rac1 (Rac1 Wt); constitutively active Rac1 Q61L ...
Alfy, a novel FYVE-domain-containing protein associated with
... (mainly) class III phosphoinositide 3-kinases (PI-3-kinases) (Vanhaesebroeck et al., 2001). This lipid is crucial for endocytic and autophagic membrane traffic (Simonsen et al., 2001) and we are beginning to learn some of the underlying molecular mechanisms. The identification of two conserved prote ...
... (mainly) class III phosphoinositide 3-kinases (PI-3-kinases) (Vanhaesebroeck et al., 2001). This lipid is crucial for endocytic and autophagic membrane traffic (Simonsen et al., 2001) and we are beginning to learn some of the underlying molecular mechanisms. The identification of two conserved prote ...
Extracting quantitative information from
... Protein separation and quantification molecular charge ...
... Protein separation and quantification molecular charge ...
Do Legume Storage Proteins Play a Role in
... The observation that legume vicilins bind to a chitin matrix led to the discovery that vicilins, from cowpea and other legumes, strongly bind to several chitin-containing structures. Among these are structures of the midgut of both C. maculatus and Z. subfasciatus (Firmino et al., 1996) and cell wal ...
... The observation that legume vicilins bind to a chitin matrix led to the discovery that vicilins, from cowpea and other legumes, strongly bind to several chitin-containing structures. Among these are structures of the midgut of both C. maculatus and Z. subfasciatus (Firmino et al., 1996) and cell wal ...
+TIPs and Microtubule Regulation. The Beginning of the Plus End in
... Potential Arabidopsis families were identified either from published reports or from database searches at NCBI. For sequences identified by database searches, designation as a putative 1TIP or interacting protein was based on the presence of conserved protein domain structures (as predicted from the ...
... Potential Arabidopsis families were identified either from published reports or from database searches at NCBI. For sequences identified by database searches, designation as a putative 1TIP or interacting protein was based on the presence of conserved protein domain structures (as predicted from the ...
Protein phosphorylation
Protein phosphorylation is a post-translational modification of proteins in which an amino acid residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or modifying its function. The reverse reaction of phosphorylation is called dephosphorylation, and is catalyzed by protein phosphatases. Protein kinases and phosphatases work independently and in a balance to regulate the function of proteins. The amino acids most commonly phosphorylated are serine, threonine, and tyrosine in eukaryotes, and histidine in prokaryotes, which play important and well-characterized roles in signaling pathways and metabolism. However, many other amino acids can also be phosphorylated, including arginine, lysine, and cysteine. Protein phosphorylation was first reported in 1906 by Phoebus Levene at the Rockefeller Institute for Medical Research with the discovery of phosphorylated vitellin. However, it was nearly 50 years until the enzymatic phosphorylation of proteins by protein kinases was discovered.