-Independent Manner Nucleus in a Cytokine
... differentiation as well as promote the survival of various hemopoietic cells (28). GM-CSFR and IL-3R consist of two subunits, ␣ and , both of which are members of the cytokine receptor superfamily (29). The ␣ subunit is specific to each cytokine, and the  subunit (c) is shared by GM-CSF, IL-3, an ...
... differentiation as well as promote the survival of various hemopoietic cells (28). GM-CSFR and IL-3R consist of two subunits, ␣ and , both of which are members of the cytokine receptor superfamily (29). The ␣ subunit is specific to each cytokine, and the  subunit (c) is shared by GM-CSF, IL-3, an ...
Plant serine/arginine-rich proteins and their role in pre
... speckles [51,63–65]. Differences in the distribution of some SR proteins within the nuclei of different cell types were also observed. The number of speckles varied depending on cell type, cell cycle stage and developmental stage [51,63]. All SR proteins were excluded from the nucleolus and Cajal bo ...
... speckles [51,63–65]. Differences in the distribution of some SR proteins within the nuclei of different cell types were also observed. The number of speckles varied depending on cell type, cell cycle stage and developmental stage [51,63]. All SR proteins were excluded from the nucleolus and Cajal bo ...
Emerging biological roles of Cl− intracellular channel proteins
... Singh et al., 2007), endosomal trafficking (Argenzio et al., 2014; Chou et al., 2016; Dozynkiewicz et al., 2012), vacuole formation and fusion (Berry et al., 2003), as well as intravesicular pH regulation (Berry et al., 2003; Jiang et al., 2012; Ulmasov et al., 2009), among others. Moreover, they sh ...
... Singh et al., 2007), endosomal trafficking (Argenzio et al., 2014; Chou et al., 2016; Dozynkiewicz et al., 2012), vacuole formation and fusion (Berry et al., 2003), as well as intravesicular pH regulation (Berry et al., 2003; Jiang et al., 2012; Ulmasov et al., 2009), among others. Moreover, they sh ...
Biogenesis of the Protein Storage Vacuole Crystalloid
... Two Arabidopsis expressed sequence tag (EST) sequences (these data are available from GenBank/EMBL/DDBJ under accession No. Z35151 and Z34589) were identified from BLAST searches (http://www.ncbi. nlm.nih.gov/) using the NH2-terminal 400–amino acid sequence of VSRAt-2 (Paris et al., 1997) as a query ...
... Two Arabidopsis expressed sequence tag (EST) sequences (these data are available from GenBank/EMBL/DDBJ under accession No. Z35151 and Z34589) were identified from BLAST searches (http://www.ncbi. nlm.nih.gov/) using the NH2-terminal 400–amino acid sequence of VSRAt-2 (Paris et al., 1997) as a query ...
Characterization of the pscC
... wound infection, and pneumonic septicaemia [2] [3]. Due to recurrent causes of nosocomial ...
... wound infection, and pneumonic septicaemia [2] [3]. Due to recurrent causes of nosocomial ...
Biogenesis of the Protein Storage Vacuole Crystalloid
... Two Arabidopsis expressed sequence tag (EST) sequences (these data are available from GenBank/EMBL/DDBJ under accession No. Z35151 and Z34589) were identified from BLAST searches (http://www.ncbi. nlm.nih.gov/) using the NH2-terminal 400–amino acid sequence of VSRAt-2 (Paris et al., 1997) as a query ...
... Two Arabidopsis expressed sequence tag (EST) sequences (these data are available from GenBank/EMBL/DDBJ under accession No. Z35151 and Z34589) were identified from BLAST searches (http://www.ncbi. nlm.nih.gov/) using the NH2-terminal 400–amino acid sequence of VSRAt-2 (Paris et al., 1997) as a query ...
G PROTEIN βγ SUBUNITS
... The earliest functions established for individual subunits of G proteins were the activation of retinal cGMP phosphodiesterase by Gαt and of adenylyl cyclase by GαS [reviewed by Gilman (1)]. Generalizing on these findings, Gα was postulated to be the effector regulatory subunit. Gβγ was thought to t ...
... The earliest functions established for individual subunits of G proteins were the activation of retinal cGMP phosphodiesterase by Gαt and of adenylyl cyclase by GαS [reviewed by Gilman (1)]. Generalizing on these findings, Gα was postulated to be the effector regulatory subunit. Gβγ was thought to t ...
3 - Dr. Jerry Cronin
... • Energy from hydrolysis of ATP causes shape change in transport protein that "pumps" solutes (ions) across membrane • E.g., calcium, hydrogen, Na+-K+ pumps ...
... • Energy from hydrolysis of ATP causes shape change in transport protein that "pumps" solutes (ions) across membrane • E.g., calcium, hydrogen, Na+-K+ pumps ...
Mast cell activation is differentially affected by heat shock
... arachidonic acid metabolites. In a previous study, we have demonstrated that heat shock activates heat shock transcription factor-1 (HSF-1), induces heat shock protein 70 (HSP70), and suppresses cytokine production in bone marrow–derived mast cells (BMMC). In this study, we further investigated the ...
... arachidonic acid metabolites. In a previous study, we have demonstrated that heat shock activates heat shock transcription factor-1 (HSF-1), induces heat shock protein 70 (HSP70), and suppresses cytokine production in bone marrow–derived mast cells (BMMC). In this study, we further investigated the ...
PCM-1, A 228-kD Centrosome Autoantigen with a Distinct Cell Cycle
... outlined by Young and Davis (1983). Production of fusion proteins was induced by overlying the plates with filters soaked in 10 mM isopropyl-/~-l>thiogaiactoside (IPTG). The plates then were incubated for 2 h at 37°C, and then the nitrocellulose filters were removed and transferred to a solution of ...
... outlined by Young and Davis (1983). Production of fusion proteins was induced by overlying the plates with filters soaked in 10 mM isopropyl-/~-l>thiogaiactoside (IPTG). The plates then were incubated for 2 h at 37°C, and then the nitrocellulose filters were removed and transferred to a solution of ...
Amino acid
... • are extremely large natural polymers. • have molecular weights of ~6000 – several million u. • are too large to pass through cell membranes. • are contained inside the normal cells where they were formed. • can leak out if cell is damaged by disease or trauma. • Protein in urine can indicate damag ...
... • are extremely large natural polymers. • have molecular weights of ~6000 – several million u. • are too large to pass through cell membranes. • are contained inside the normal cells where they were formed. • can leak out if cell is damaged by disease or trauma. • Protein in urine can indicate damag ...
Immunodetection of the expression of microsomal proteins encoded
... lung (Figure 3, lanes 1, 6 and 7 respectively) did not contain the major immunoreactive band found in liver and kidney microsomes. It should be noted that microsomes from HEK-293, COS7, and CHO-K1 cells also did not contain the hepatic immunoreactive band (see Figures 2A, 2B and 2C). Skeletal muscle ...
... lung (Figure 3, lanes 1, 6 and 7 respectively) did not contain the major immunoreactive band found in liver and kidney microsomes. It should be noted that microsomes from HEK-293, COS7, and CHO-K1 cells also did not contain the hepatic immunoreactive band (see Figures 2A, 2B and 2C). Skeletal muscle ...
Role of N-linked oligosaccharide chains in the processing and
... natural consensus site, we performed oligonucleotide-directed mutagenesis using a single oligonucleofide designed to produce the required change. The combinations of mutation sites were generated by performing successive site-directed mutagenesis procedures on previously mutagenized templates. Confi ...
... natural consensus site, we performed oligonucleotide-directed mutagenesis using a single oligonucleofide designed to produce the required change. The combinations of mutation sites were generated by performing successive site-directed mutagenesis procedures on previously mutagenized templates. Confi ...
The fission yeast Schizosaccharomyces pombe has two distinct
... Besides tRNase Z, a few members of the MBL superfamily with either demonstrated or predicted nuclease activity have been found to participate in nucleic-acid metabolism. Most of these proteins belong to the β-CASP (MBL associated CISF Artemis SNM1/PSO2) family. The best studied member of this family ...
... Besides tRNase Z, a few members of the MBL superfamily with either demonstrated or predicted nuclease activity have been found to participate in nucleic-acid metabolism. Most of these proteins belong to the β-CASP (MBL associated CISF Artemis SNM1/PSO2) family. The best studied member of this family ...
Cell biology # 2 - Nutley Public Schools
... • Energy from hydrolysis of ATP causes shape change in transport protein that "pumps" solutes (ions) across membrane • E.g., calcium, hydrogen, Na+-K+ pumps ...
... • Energy from hydrolysis of ATP causes shape change in transport protein that "pumps" solutes (ions) across membrane • E.g., calcium, hydrogen, Na+-K+ pumps ...
MAPK cascade signalling networks in plant defence
... MPK4. The WRKY53 transcription factor may be partially responsible for the mekk1-specific gene set, as MEKK1 directly interacts with WRKY53 and alters the activity of this transcription factor [39]—a unique short cut of MAPK signalling. The interaction of MKK1-MPK4 and MKK2-MPK4 was lately confirme ...
... MPK4. The WRKY53 transcription factor may be partially responsible for the mekk1-specific gene set, as MEKK1 directly interacts with WRKY53 and alters the activity of this transcription factor [39]—a unique short cut of MAPK signalling. The interaction of MKK1-MPK4 and MKK2-MPK4 was lately confirme ...
Amino acid substitutions that specifically impair the transcriptional
... specific to the E2 TAD or one shared with other transactivation domains, we performed affinity chromatography with the acidic activation domains of herpes simplex (HSV) VP16 and of the tumor suppressor protein p53. These proteins were purified from bacteria as fusions with GST (Fig. 3B), and immobil ...
... specific to the E2 TAD or one shared with other transactivation domains, we performed affinity chromatography with the acidic activation domains of herpes simplex (HSV) VP16 and of the tumor suppressor protein p53. These proteins were purified from bacteria as fusions with GST (Fig. 3B), and immobil ...
Bilayers as Protein Solvents: Role of Bilayer Structure and Elastic
... In plasma membranes, the strong interactions between cholesterol and the saturated hydrocarbon chains of SM cause the formation of lipid rafts. As noted above, raft bilayers composed of SM:cholesterol have different elastic properties than the surrounding matrix (nonraft) bilayer enriched in unsatur ...
... In plasma membranes, the strong interactions between cholesterol and the saturated hydrocarbon chains of SM cause the formation of lipid rafts. As noted above, raft bilayers composed of SM:cholesterol have different elastic properties than the surrounding matrix (nonraft) bilayer enriched in unsatur ...
P1 and P2 protein heterodimer binding to the P0 protein of
... The stalk is a functional domain of the large ribosomal subunit that is directly involved in the interaction and GTPase activity of several soluble factors during translation (1). In eukaryotes, the stalk is formed by a central 32-kDa protein, P0, which interacts through its N-terminal domain (NTD) ...
... The stalk is a functional domain of the large ribosomal subunit that is directly involved in the interaction and GTPase activity of several soluble factors during translation (1). In eukaryotes, the stalk is formed by a central 32-kDa protein, P0, which interacts through its N-terminal domain (NTD) ...
Presence and Absence of COX8 in Reptile Transcriptomes
... as ultimate product (Figure 1). The major proteins in the system are NADH dehydrogenase, succinate dehydrogenase, cytochrome b, and cytochrome oxidase c, all of which assemble into multisubunit complexes. Unsurprisingly, mitochondria appear to be involved in several process related to aerobic perfor ...
... as ultimate product (Figure 1). The major proteins in the system are NADH dehydrogenase, succinate dehydrogenase, cytochrome b, and cytochrome oxidase c, all of which assemble into multisubunit complexes. Unsurprisingly, mitochondria appear to be involved in several process related to aerobic perfor ...
6 systems biology of cell organization
... The genome of every organism contains the information necessary to produce a system of RNA and protein molecules that provides the foundation for cell structure, function, and organization. An important paradigm in biology is that “structure determines function.” The information in most genes is use ...
... The genome of every organism contains the information necessary to produce a system of RNA and protein molecules that provides the foundation for cell structure, function, and organization. An important paradigm in biology is that “structure determines function.” The information in most genes is use ...
Chapter 28 Discovery and Classification of Glycan
... function for lectins in many organisms. In eukaryotic cells, including yeast as well as higher eukaryotes, several groups of lectins are important in glycoprotein biosynthesis and intracellular movement (Chapter 39). In the endoplasmic reticulum (ER), two lectins, calnexin and calreticulin bind m ...
... function for lectins in many organisms. In eukaryotic cells, including yeast as well as higher eukaryotes, several groups of lectins are important in glycoprotein biosynthesis and intracellular movement (Chapter 39). In the endoplasmic reticulum (ER), two lectins, calnexin and calreticulin bind m ...
Role for Adenosine Triphosphate in Regulating the Assembly and
... Labeling of Ceils. Cells were maintained, infected with VSV, and labeled with [35S]methionine as previously described in detail (Balch et al., 1986). Briefly, confluent monolayers of 15B cells were infected with either wildtype or ts045 VSV. After 45 min at 32~ the cells were placed in a 37"C (wild ...
... Labeling of Ceils. Cells were maintained, infected with VSV, and labeled with [35S]methionine as previously described in detail (Balch et al., 1986). Briefly, confluent monolayers of 15B cells were infected with either wildtype or ts045 VSV. After 45 min at 32~ the cells were placed in a 37"C (wild ...
- Wiley Online Library
... classic BEVS is a time-, labor-, and cost-intensive (Jarvis, 2014) process as sufficient recombinant baculoviral material of adequate quality for each construct is required for analysis of the expression efficiency. Hence, screening target constructs in a plasmid based and automated miniaturized expre ...
... classic BEVS is a time-, labor-, and cost-intensive (Jarvis, 2014) process as sufficient recombinant baculoviral material of adequate quality for each construct is required for analysis of the expression efficiency. Hence, screening target constructs in a plasmid based and automated miniaturized expre ...
Protein phosphorylation
Protein phosphorylation is a post-translational modification of proteins in which an amino acid residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or modifying its function. The reverse reaction of phosphorylation is called dephosphorylation, and is catalyzed by protein phosphatases. Protein kinases and phosphatases work independently and in a balance to regulate the function of proteins. The amino acids most commonly phosphorylated are serine, threonine, and tyrosine in eukaryotes, and histidine in prokaryotes, which play important and well-characterized roles in signaling pathways and metabolism. However, many other amino acids can also be phosphorylated, including arginine, lysine, and cysteine. Protein phosphorylation was first reported in 1906 by Phoebus Levene at the Rockefeller Institute for Medical Research with the discovery of phosphorylated vitellin. However, it was nearly 50 years until the enzymatic phosphorylation of proteins by protein kinases was discovered.