Scanning transmission soft x-ray microscopy at beamline X
... redox-reactions on iron oxides with high spatial resolution at X-1A. ...
... redox-reactions on iron oxides with high spatial resolution at X-1A. ...
Extending the Effective Ranging Depth of Spectral Domain Optical
... MEMS-Vertical Cavity Surface Emitting Laser (VCSEL) source-based [3] SSOCT system was developed for in vivo high-speed imaging of the eye with an unprecedented imaging depth of 50 mm in air and little sensitivity loss. The SDOCT system based on the orthogonal dispersion spectrometer [4] or the frequ ...
... MEMS-Vertical Cavity Surface Emitting Laser (VCSEL) source-based [3] SSOCT system was developed for in vivo high-speed imaging of the eye with an unprecedented imaging depth of 50 mm in air and little sensitivity loss. The SDOCT system based on the orthogonal dispersion spectrometer [4] or the frequ ...
Using Transmission Electron Microscopy (TEM) for Chemical
... macromolecules. An effective resolution of approximately 2nm is achievable as limited by the specimen preparation and radiation damage. Although the STEM cannot provide primary or secondary structure of proteins (a goal still within reach of high-resolution CTEM) it provides valuable information abo ...
... macromolecules. An effective resolution of approximately 2nm is achievable as limited by the specimen preparation and radiation damage. Although the STEM cannot provide primary or secondary structure of proteins (a goal still within reach of high-resolution CTEM) it provides valuable information abo ...
PDF
... muscle layers at ultrahigh resolution. The cheek pouch was index matched by using a microscope cover glass and saline solution. While this approach achieves high resolutions in the important 1.3 µm wavelength range, there are several disadvantages. The femtosecond Ti:Sapphire laser used to generate ...
... muscle layers at ultrahigh resolution. The cheek pouch was index matched by using a microscope cover glass and saline solution. While this approach achieves high resolutions in the important 1.3 µm wavelength range, there are several disadvantages. The femtosecond Ti:Sapphire laser used to generate ...
PDF
... measurements. This approach is referred to as Fourier transform light scattering, as it is the spatial analogue to Fourier transform spectroscopy [12]. The range of QPI applications in biology includes red blood cell imaging [2–4], cell growth [3,13], cell refractive index [14,15], and optical prope ...
... measurements. This approach is referred to as Fourier transform light scattering, as it is the spatial analogue to Fourier transform spectroscopy [12]. The range of QPI applications in biology includes red blood cell imaging [2–4], cell growth [3,13], cell refractive index [14,15], and optical prope ...
Biomedical imaging in the undergraduate physics curriculum
... prominent imaging modalities. The course covers optical microscopy—a ubiquitous tool in many branches of science— and medical imaging techniques such as ultrasonography, computed (axial) tomography (CT), and magnetic resonance imaging (MRI). This combination of topics broadens the appeal of the cour ...
... prominent imaging modalities. The course covers optical microscopy—a ubiquitous tool in many branches of science— and medical imaging techniques such as ultrasonography, computed (axial) tomography (CT), and magnetic resonance imaging (MRI). This combination of topics broadens the appeal of the cour ...
TEM - UiO
... If a sample is crystalline, many of the electrons will undergo elastic scattering from the various (hkl) planes. This scattering produces many diffracted beams. If any of these diffracted beams is allowed to pass through the objective aperture a dark field image is obtained. In order to reduce spher ...
... If a sample is crystalline, many of the electrons will undergo elastic scattering from the various (hkl) planes. This scattering produces many diffracted beams. If any of these diffracted beams is allowed to pass through the objective aperture a dark field image is obtained. In order to reduce spher ...
Spectral Domain Optical Coherence Tomography
... ultrasound imaging, except that it uses light instead of sound.1 The interface between different ocular tissues can be determined by changes in reflective properties between the tissues. Current experimental ophthalmic OCT instruments provide more structural information than any other ophthalmic dia ...
... ultrasound imaging, except that it uses light instead of sound.1 The interface between different ocular tissues can be determined by changes in reflective properties between the tissues. Current experimental ophthalmic OCT instruments provide more structural information than any other ophthalmic dia ...
This space should be left blank, except for the name of the first author.
... GHz) are split into 8 equally spaced frequency bands (0.7 GHz bandwidth), which corresponds to 8 different radial locations in the plasma. The radially resolved IF signals are integrated (video bandwidth ~ 400 kHz) and finally sampled by the digitizers (up to 2 MHz). The total number of channels is ...
... GHz) are split into 8 equally spaced frequency bands (0.7 GHz bandwidth), which corresponds to 8 different radial locations in the plasma. The radially resolved IF signals are integrated (video bandwidth ~ 400 kHz) and finally sampled by the digitizers (up to 2 MHz). The total number of channels is ...
MEMS-based handheld confocal microscope for in
... Since this initial investigation, the development of confocal laser scanning microscopes (CLSM) has allowed for increased optical power and specific wavelength selection. Because the primary application for confocal skin imaging is as a potential alternative to biopsy, many experiments have compared ...
... Since this initial investigation, the development of confocal laser scanning microscopes (CLSM) has allowed for increased optical power and specific wavelength selection. Because the primary application for confocal skin imaging is as a potential alternative to biopsy, many experiments have compared ...
12. confocal microscopy.
... The principle of confocal microscopy was described prior to the invention of lasers [Ref Minsky Patent 1957]. However, today most confocal systems use lasers for illumination. The confocal imaging system can operate either in transmission or reflection, as shown in Fig. 1. In both cases, the out of ...
... The principle of confocal microscopy was described prior to the invention of lasers [Ref Minsky Patent 1957]. However, today most confocal systems use lasers for illumination. The confocal imaging system can operate either in transmission or reflection, as shown in Fig. 1. In both cases, the out of ...
phase retrieval by using transport-of
... Reconstructing the phase of a complex field given its intensity is a fundamental problem in bioimaging. Most cells and soft tissues are highly transparent. Thus, without staining or tagging, they generate very low contrast intensity images. This implies that they are barely visible under a standard ...
... Reconstructing the phase of a complex field given its intensity is a fundamental problem in bioimaging. Most cells and soft tissues are highly transparent. Thus, without staining or tagging, they generate very low contrast intensity images. This implies that they are barely visible under a standard ...
Get PDF - OSA Publishing
... [17], which compare the physical principles of various nanoscopy methods. The fundamental criteria for achieving superresolution in continuously labeled samples are lucidly discussed by Heintzmann and Gustafsson [18], and this is particularly relevant to SIM and to the conceptually related STED tech ...
... [17], which compare the physical principles of various nanoscopy methods. The fundamental criteria for achieving superresolution in continuously labeled samples are lucidly discussed by Heintzmann and Gustafsson [18], and this is particularly relevant to SIM and to the conceptually related STED tech ...
Tutor 4
... given equal line/space pitch will result in a potentially dramatic increase in the DOF of that feature! Phase shifting masks, in some applications, improve depth-of-focus by the same mechanism as off-axis illumination. For example, the use of alternating shifters on an equal line/space pattern (call ...
... given equal line/space pitch will result in a potentially dramatic increase in the DOF of that feature! Phase shifting masks, in some applications, improve depth-of-focus by the same mechanism as off-axis illumination. For example, the use of alternating shifters on an equal line/space pattern (call ...
High-speed optical frequency-domain imaging
... detectable reflectivity). As the A-line rate increases, the detection bandwidth should be increased proportionally, and therefore the sensitivity drops [4]. The sensitivity of state-ofthe-art time-domain OCT systems operating at 2-kHz ranges between −105 and −110 dB. Most biomedical applications req ...
... detectable reflectivity). As the A-line rate increases, the detection bandwidth should be increased proportionally, and therefore the sensitivity drops [4]. The sensitivity of state-ofthe-art time-domain OCT systems operating at 2-kHz ranges between −105 and −110 dB. Most biomedical applications req ...
Upholding the diffraction limit in the focusing of light and sound
... The concept of the diffraction limit put forth by Ernst Abbe and others has been an important guiding principle limiting our ability to tightly focus classical waves, such as light and sound, in the far field. In the past decade, numerous reports have described focusing or imaging with light and sou ...
... The concept of the diffraction limit put forth by Ernst Abbe and others has been an important guiding principle limiting our ability to tightly focus classical waves, such as light and sound, in the far field. In the past decade, numerous reports have described focusing or imaging with light and sou ...
A high resolution ion microscope for cold atoms
... previous lens. The position of the image planes is determined by simulating different ion trajectories coming from a common starting point and determining the intersection of the trajectories behind the lens. There are of course several voltage settings for the different lenses to achieve a sharp im ...
... previous lens. The position of the image planes is determined by simulating different ion trajectories coming from a common starting point and determining the intersection of the trajectories behind the lens. There are of course several voltage settings for the different lenses to achieve a sharp im ...
Supplementary Methods and References
... length (OPL)) for light passing through the entire sample is measured. Figures S3C-E show the corresponding drOPD maps at three optical depths (zopl = 1, 2, and 5 µm). At a superficial optical depth of 1 µm (Fig. S3C), the drOPD map shows both cells and polymer network. At an optical depth of 2 µm ( ...
... length (OPL)) for light passing through the entire sample is measured. Figures S3C-E show the corresponding drOPD maps at three optical depths (zopl = 1, 2, and 5 µm). At a superficial optical depth of 1 µm (Fig. S3C), the drOPD map shows both cells and polymer network. At an optical depth of 2 µm ( ...
The Optical Design of Miniaturized Microscope Objective for CARS
... been highlighted for visualizing the morphological details of living tissue which cannot be resolved by ultrasound or magnetic resonance imaging. NLO microscopy utilizes the signal from two-photon excitation fluorescence (TPEF) [1-5], second harmonic generation (SHG) [6-7], sum frequency generation ...
... been highlighted for visualizing the morphological details of living tissue which cannot be resolved by ultrasound or magnetic resonance imaging. NLO microscopy utilizes the signal from two-photon excitation fluorescence (TPEF) [1-5], second harmonic generation (SHG) [6-7], sum frequency generation ...
Fourier Transforms and Images
... scattered into diffracted beams. b) The phase of the electrons is changed as they go through the sample. They have a different kinetic energy in the sample, this changes the wavelength, which in turn changes the phase. ...
... scattered into diffracted beams. b) The phase of the electrons is changed as they go through the sample. They have a different kinetic energy in the sample, this changes the wavelength, which in turn changes the phase. ...
PDF - Grueber Lab
... respect to the objectives for volumetric imaging8,9. Only one other light-sheet technique has been implemented through a single objective, although volumetric imaging still required the use of piezoelectric objective scanning with a limited field of view10. In all cases, piezoelectric objective scan ...
... respect to the objectives for volumetric imaging8,9. Only one other light-sheet technique has been implemented through a single objective, although volumetric imaging still required the use of piezoelectric objective scanning with a limited field of view10. In all cases, piezoelectric objective scan ...
On the diffraction limit for lensless imaging
... adopt an intermediate best value of u. This seemed inadvisable as it might be misinterpreted as an improvement or refinement of the results obtained by Petzval and Rayleigh. The above analysis has shown that both are valid and that there is a fairly wide range of M'S which give acceptably sharp imag ...
... adopt an intermediate best value of u. This seemed inadvisable as it might be misinterpreted as an improvement or refinement of the results obtained by Petzval and Rayleigh. The above analysis has shown that both are valid and that there is a fairly wide range of M'S which give acceptably sharp imag ...
Non-invasive ophthalmic imaging of adult zebrafish eye using
... Keywords: Eyes, non-invasive ophthalmic imaging, optical coherence tomography, zebrafish. USE of optical techniques for biomedical imaging is a topic of considerable current interest. This is motivated by the potential of optical techniques to provide sub-millimetre resolution imaging without the ne ...
... Keywords: Eyes, non-invasive ophthalmic imaging, optical coherence tomography, zebrafish. USE of optical techniques for biomedical imaging is a topic of considerable current interest. This is motivated by the potential of optical techniques to provide sub-millimetre resolution imaging without the ne ...