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Light Microscopy [10 credits]
Light Microscopy [10 credits]

...  Explain the first set of important components of a confocal laser scanning microscope: o Lasers as excitation light sources o Beam splitters as filtering devices that separate excitation from emitted light o Objective lenses as the central optical components for image magnification Lecture 3 Title ...
Biology 177: Principles of Modern Microscopy
Biology 177: Principles of Modern Microscopy

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PDF of this page - Kettering University Catalog
PDF of this page - Kettering University Catalog

... Terms Offered: Summer, Fall The phenomena of vibration and waves provide a fundamental background necessary to approach a wide variety of applications in physics and engineering. The first part of this course will introduce students to the basics of vibration, including the effects of real damping, ...
Soft X-ray tomography and cryogenic light microscopy - X
Soft X-ray tomography and cryogenic light microscopy - X

... Our understanding of cell structure and behaviour has been particularly dependent on imaging. As a consequence, the emergence of new imaging modalities potentially leads to new insights and discoveries in cell biology. In this review, we examine soft X-ray tomography (SXT), an emerging technique for ...
Laser Medicine and Medical Imaging – J. G. Fujimoto
Laser Medicine and Medical Imaging – J. G. Fujimoto

... advantage that it is fiber optically based and can readily be integrated with a wide range of existing clinical imaging instruments including microscopes, laparoscopes, endoscopes, and catheters. Several imaging devices have been designed and developed and evaluated in both animal and preliminary cl ...
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[pdf]

... must be done carefully because the integrals involving the gradient of the Green's function are not continuous when the interior point approaches a point on the surface. The other integrals involving the Green's function however are continuous [21]. From an experimental point of view, if the photon ...
Aberration-free three-dimensional multiphoton imaging of neuronal
Aberration-free three-dimensional multiphoton imaging of neuronal

... neurons in a cortical slab (Fig. 6A). Neurons, about 200–300 μm below the cortical surface, were filled with the calcium-sensitive fluorescent dye Fluo-4 and the fluorescent structural dye Alexa 594 to visualize the fine structure of neuronal processes. Dendritic activity could then be monitored by ...
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Iterative reconstruction algorithm for optoacoustic imaging

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Analysis of the detective quantum efficiency of
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... quantain one pixel or minimum resolving element is determinedfor each stageas the product of gains and efficiencies of all precedingstages.This information can be displayedin a system nomogram,showing the number of quantaon the vertical ‘axis for each stage.2”The stage with the fewest quanta (N,,,,) ...
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... recovery. Reduced sublingual blood flow has been qualitatively observed in sepsis patients using orthogonal polarization microscopy (OPM) which utilizes a polarized light to illuminate the area of interest and to view the reflected light from tissue surfaces. In order to reduce surface reflection an ...
Phase contrast microscopy (PCM) represents a major breakthrough
Phase contrast microscopy (PCM) represents a major breakthrough

... Equation 15 states that imaging a phase object produces an intensity image that is constant across the plane, i.e. the image has zero contrast. For this reason, imaging transparent specimens such as live cells is very challenging. Developing clever methods for generating contrast of phase objects h ...
COLLEGE OF SCIENCE
COLLEGE OF SCIENCE

... Students who might elect to take the course: Graduate Students in the College of Science or College of Engineering ...
Imaging properties of supercritical angle
Imaging properties of supercritical angle

... Abstract: In recent years, new optical systems have been developed with the ability to collect light at very high angles of emission, exceeding the critical angle of total internal reflection. Prominent examples are solid-immersion lenses and paraboloid collectors. These systems achieve high efficie ...
Compact Adaptive Optics Line Scanning Ophthalmoscope
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... optical access that AO provides to as many research and clinical applications as possible. The instrument is designed to fill the niche between SLO instruments that provide wide field but limited resolution for routine clinical use and the complex, high resolution, highperformance AO instruments tha ...
Ultrahigh-resolution optical coherence tomography
Ultrahigh-resolution optical coherence tomography

... imaging [3], but they currently have limited output power of a few mW, which is insufficient for many imaging applications. In order to increase the axial resolution of OCT imaging, broadband mode-locked lasers have been used. Both broadband light directly from a laser [47] and laser light externall ...
Diffuse optical imaging
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... provide clinically useful information by examination of the absorption of tissue, provided that the confounding effects of scatter were minimized, by examining either small body parts (e.g. oximetry of the finger or earlobe) or exploiting conditions indicated by low scatter (e.g. hydrocephalus and hy ...
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... Remember the radiometric unit L = radiance ( or photometric “brightness”) in units of [W/(sr m2)]? We just found that as size of an object goes up, it’s angular extent decreases by the same amount. Brightness is conserved. ...
Enhancement of image quality and imaging depth with Airy light
Enhancement of image quality and imaging depth with Airy light

... the phase profile is described by P(u, v) = exp (2πiα[u 3 + v 3 ]), where u and v are normalised pupil coordinates corresponding to the z− and y−axes respectively and α allows the propagationinvariance of the Airy beam to be tuned [9]. The SLM was imaged onto an acousto-optic deflector (AOD; Neos AO ...
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IMAGING WITH THZ PULSES TX 7725
IMAGING WITH THZ PULSES TX 7725

... Until the 198Os, the use of electromagnetic waves in the far-infrared, or terahertz (THz), region of the spectrum was limited due to the low intensity of thermal sources and the poor sensitivity of most detectors. Many of these difficulties were overcome by the introduction of THz time-domain spectr ...
Partially coherent image formation with x
Partially coherent image formation with x

... 11a2. Polychromatic synchrotron radiation is focused onto the sample by a condenser zone plate, and an objective zone plate forms an enlarged high-resolution image of the object. This image is detected with an x-ray CCD camera. Because zone plates are diffraction optics, they have a strong chromatic ...
PDF file. - UCL Medical Physics and Biomedical
PDF file. - UCL Medical Physics and Biomedical

... optical energy at a specific point is linearly dependent on the absorption spectra of the chromophores at that point [1-3]. The wavelength dependent absorption and scattering in the space surrounding a specific point will affect the detected absorbed energy spectrum, which compromises the use of sim ...
C. Huang, X. Wu, H. Liu, B. Aldalali, J.A. Rogers and H. Jiang
C. Huang, X. Wu, H. Liu, B. Aldalali, J.A. Rogers and H. Jiang

... micro-square-tubes fabricated in a planar layout on the SOI substrate, picture of the hemispherical profile of our device packaged with a 3-D printed plastic casing of matching radii, and a scanning electron microscope (SEM) image highlighting the hemispherical surface profile of the artificial RSCE ...
6,
6,

... is presented. This system can be used to work in white light. The holographic optical elements (holographic lenses) are made as thick phase holograms on silver halide sensitized gelatin (SHSG) and they present a maximum diffraction efficiency of 75 %. Geometrical conditions at reconstruction with co ...
< 1 ... 6 7 8 9 10 11 12 13 14 16 >

Preclinical imaging

Preclinical imaging is the visualization of living animals for research purposes, such as drug development. Imaging modalities have long been crucial to the researcher in observing changes, either at the organ, tissue, cell, or molecular level, in animals responding to physiological or environmental changes. Imaging modalities that are non-invasive and in vivo have become especially important to study animal models longitudinally. Broadly speaking, these imaging systems can be categorized into primarily morphological/anatomical and primarily molecular imaging techniques. Techniques such as high-frequency micro-ultrasound, magnetic resonance imaging (MRI) and computed tomography (CT) are usually used for anatomical imaging, while optical imaging (fluorescence and bioluminescence), positron emission tomography (PET), and single photon emission computed tomography (SPECT) are usually used for molecular visualizations.These days, many manufacturers provide multi-modal systems combining the advantages of anatomical modalities such as CT and MR with the functional imaging of PET and SPECT. As in the clinical market, common combinations are SPECT/CT, PET/CT and PET/MR.
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