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Matrix assisted laser desorption/ionization
Matrix assisted laser desorption/ionization

... (turnip sawfly) was monitored using MALDI-MSI. MS images of longitudinal cryo-sections of these larvae revealed the rapid sequestration and concentration of sinalbin in the haemolymph, rather than gut, as a strategy to detoxify ingested leaf ...
Photoacoustic imaging velocimetry for flow
Photoacoustic imaging velocimetry for flow

... multimodal photoacoustic and photothermal high-contrast molecular agents,” Nat. Nanotechnol. 4(10), 688–694 ...
as a PDF - Department of Engineering Science
as a PDF - Department of Engineering Science

... We also note that rays from the object space are mapped onto conjugate rays with the same angle in image space, in accordance with Eq. (2). As such it is possible to see that the limiting angular aperture in the system cmax will essentially limit the overall resolution of the instrument. The effecti ...
Live Data Mode
Live Data Mode

... plane, and reduced phototoxicity are the properties of multiphoton microscopy that enable visualization of deep structures. Brain slices, for example, are highly scattering and are usually sliced several hundred micrometers thick. Therefore it is very difficult to image brain slices using a standard ...
Ref_Note_final092911
Ref_Note_final092911

... What about space charge limitation on PEEM experiments with FEL sources ? Since there have been no PEEM experiments at FELs so far, there is no work to reference here. However, there has been work using ultrafast UV laser pumped PEEM, which is relevant to potential FEL experiments. We have added the ...
doc - University of Rochester
doc - University of Rochester

... wavelength of the idler photon; however, because the magnification depends on the two wavelengths, the resolution of a pair of point scatters on the object depends on the wavelength both photons. In cases in which the position of the object can be controlled, the minimum resolvable distance can be o ...
Optical Coherence Tomography as a diagnostic tool
Optical Coherence Tomography as a diagnostic tool

... coherence length of the source. Hence, the axial resolution in OCT is proportional to the temporal coherence length of the source. OCT is an imaging technique that fills the gap between confocal microscopy and ultra-sound imaging. The typical high resolutions offered by OCT are 5−15 µm although the ...
TO THE POSSIBILITY OF CALCULATION
TO THE POSSIBILITY OF CALCULATION

... ray theory) fail to explain this fact and further research has yet to be made. This work ascribes this capability to a combination of spatial diversity of the information and cooperative detection, and aims to explore its usage in imaging systems. We have developed a method that uses spatial diversi ...
A tutorial for designing fundamental imaging systems
A tutorial for designing fundamental imaging systems

... (2) Image sensor ( Photo detector ) Image sensor transfers light energy to electrical energy. There are various kinds of sensors such as CMOS and CCD sensors, and it provides with each merit.[3][8] CCD or CMOS sensor is used for imaging devices such as digital camera or digital photo copier. PMT is ...
Advances in diamond-turned surfaces enable unique cost
Advances in diamond-turned surfaces enable unique cost

... Imaging Spectroscopy or Hyperspectral Imaging has become a powerful technique for many fields such as remote sensing. Early spectrometers such as the Multispectral Scanner flown on the Landsat series of satellites had limited spectral and spatial resolution; 6 wavelength and 4 spatial bands1. More r ...
Resolution and Sampling Considerations
Resolution and Sampling Considerations

... frequency of 2 samples per cycle is a theoretical minimum sampling rate to resolve an object of 1 cycle in size. In reality, many more samples are usually necessary to actually “resolve” the object. Most microscopists use a sampling rate of between 3-10 samples per object as a rule of thumb (7). Thi ...
pupil function - UCT Digital Image Processing
pupil function - UCT Digital Image Processing

... CCDs can be scanned at television rates (25 frames per second) or much more slowly. Since they can integrate for periods of seconds to hours to create low-light images, they are used in astronomy and florescence microscopy, for example. The long integration times require that the sensor be cooled to ...
Adiabatic far-field sub-diffraction imaging ARTICLE Hu Cang *, Alessandro Salandrino
Adiabatic far-field sub-diffraction imaging ARTICLE Hu Cang *, Alessandro Salandrino

... lthough a tsunami is devastating near land, it is barely noticeable in the open ocean. This is because the sea floor near the land slows down the tsunami, compressing its wavelength from hundreds of kilometres1 to metres and rapidly increasing its amplitude to be destructive. Recently, a similar opti ...
Telescopes for CCD Imaging
Telescopes for CCD Imaging

... Mirror shift can contribute to this problem. SCT’s aren’t very easy to collimate. “Bob’s knobs” can help make collimation easier. The combination of features described above make the SCT one of the more difficult telescope designs to use in CCD imaging. Quality control in SCT manufacture has been a ...
Opto-acoustic Imaging
Opto-acoustic Imaging

... conventional techniques can be overcome. Such limitations include contact, frequency range and size constraints of the transducer. Second, the combination of laser and ultrasound necessitates new imaging technologies that may provide information related to both the optical and the acoustical propert ...
Chapter 1 notes- Intro to anatomy
Chapter 1 notes- Intro to anatomy

... magnetic field when magnet is activated. Then the hydrogen atoms in the body's water align with the magnet and a radio frequency is emitted to misalign them. As they realign with the magnet a radio wave is emitted from them. Sensors detect the waves and the computer takes these signals and produces ...
X-ray phase contrast microscopy at 300 nm
X-ray phase contrast microscopy at 300 nm

... contributing to the achievable contrast and the resolution in propagation-based phase contrast imaging are the degree of coherence of the incoming beam (related to the source size and the distance source-sample) and the detector resolution [7]. Therefore phase contrast x-ray imaging in propagation i ...
Nineteen Ways to do 3-Dimensional Imaging
Nineteen Ways to do 3-Dimensional Imaging

... the scene. Without stepping into this discussion, I’m going to take the broader view for this list that the techniques can provide 3D information about either a volume (volumetric imaging) or a surface (topographical imaging). There are blends of two or more 3D imaging techniques. For example, two c ...
[pdf]
[pdf]

... those of conventional diffraction tomography.7 The near-field diffraction tomography method has attracted the attention of a few researchers in the photon-migration field.8 In this Letter present a rigorous account of the theory and provide the f irst experimental images of absorbing and scattering ...
P2SF: Physically-based Point Spread Function for
P2SF: Physically-based Point Spread Function for

... example, depth estimation [1] and image restoration [2]. On the other hand, this approach does not provide an accurate representation of aberrated optical system due to the lack of physical foundation and the limited parametric description of the PSF, which is based on simple functions , such as pil ...
in phase - RIT Center for Imaging Science
in phase - RIT Center for Imaging Science

... • Physical optics, or wave optics, is the study of how light interacts with objects similar in size to its wavelength. – Light energy travels as a wave (not a ray). – Wave optics concerns the characteristics of light such as wavelength, intensity, phase, and orientation. ...
Nano-optical Imaging using Scattering Scanning Near-Field Optical Microscopy
Nano-optical Imaging using Scattering Scanning Near-Field Optical Microscopy

... We investigate a nano-imaging technique, known as scattering scanning near-field optical microscopy (s-SNOM) and image several different materials using said technique. We report our data provide potential paths for future work. I. INTRODUCTION Scientists have long studied optical spectroscopy due t ...
Multiple wavelength diffractive imaging - X
Multiple wavelength diffractive imaging - X

... $ 1300 array of 20 "m pixels. The distance between the CCD and the sample is 6.5 cm. To further reduce the scattered light an aperture with diameter of 5 mm is placed directly downstream from the sample. A monochromator was not used in this experiment and so the illumination contained several harmon ...
Optical Coherence Tomography Brochure
Optical Coherence Tomography Brochure

... This book gives a broad treatment of the subject which will include 1)the optics, science, and physics needed to understand the technology 2)a description of applications with a critical look at how the technology will successfully address actual clinical need, and 3) a discussion of delivery of OCT ...
Photoacoustic microscopy with 2
Photoacoustic microscopy with 2

... Existing photoacoustic methods for studying individual cells are mainly limited to a sensing technique called photoacoustic flow cytometry.12 It monitors photoacoustic signals from individual pigmented cells at a fixed location in blood or lymph vessels and promises to detect early-stage cancer meta ...
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Preclinical imaging

Preclinical imaging is the visualization of living animals for research purposes, such as drug development. Imaging modalities have long been crucial to the researcher in observing changes, either at the organ, tissue, cell, or molecular level, in animals responding to physiological or environmental changes. Imaging modalities that are non-invasive and in vivo have become especially important to study animal models longitudinally. Broadly speaking, these imaging systems can be categorized into primarily morphological/anatomical and primarily molecular imaging techniques. Techniques such as high-frequency micro-ultrasound, magnetic resonance imaging (MRI) and computed tomography (CT) are usually used for anatomical imaging, while optical imaging (fluorescence and bioluminescence), positron emission tomography (PET), and single photon emission computed tomography (SPECT) are usually used for molecular visualizations.These days, many manufacturers provide multi-modal systems combining the advantages of anatomical modalities such as CT and MR with the functional imaging of PET and SPECT. As in the clinical market, common combinations are SPECT/CT, PET/CT and PET/MR.
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