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Noise-equivalent sensitivity of photoacoustics
Noise-equivalent sensitivity of photoacoustics

... absorption and the low scattering of ultrasound. Clinically, photoacoustic tomography is being used to add optical contrast to ultrasound imaging of breast cancer, due to its ability to image with acoustic resolution at depths beyond the optical diffusion limit, down to 2 cm demonstrated in vivo2 an ...
Spectroscopic Imaging using Terahertz Time-Domain Signals
Spectroscopic Imaging using Terahertz Time-Domain Signals

... a freely propagating THz wave via ultrafast gating of a photoconductive switch [1-4]. The resulting electromagnetic pulse is broadband, spanning from below 100 GHz up to several THz. Photoconductive or electrooptic sampling techniques eliminate the need for cumbersome cryogenics for detection of the ...
Preview of “ZEISS Microscopy Online ...Aperture and Resolution”
Preview of “ZEISS Microscopy Online ...Aperture and Resolution”

... is  the  limiting  factor  in  achieving  numerical  apertures  greater  than  1.0.  Therefore,  in  order  to obtain  higher  working  numerical  apertures,  the  refractive  index  of  the  medium  between  the  front lens  of  the  objective  and  the  specimen  cover  slip  must  be  increased. ...
An Introduction to Propagation, Time Reversal and Imaging in
An Introduction to Propagation, Time Reversal and Imaging in

... or the Neumann condition, but not both in general. In a sense, Kirchhoff’s formula is not self-consistent but it is a good approximation when the aperture is sufficiently large and the field point is sufficiently away from the aperture/screen as compared with the wavelength and when the diffraction ...
Download PDF
Download PDF

... more inhomogeneous the tissue, the stronger the scattering. For homogeneous tissue, i.e., zero variance, ls becomes infinite, which indicates the absence of scattering. On the other hand, the g–ϕ formula contains the gradient of the phase. Generally, a phase gradient relates to a tilt in the directi ...
Comparison of Control Algorithms for a MEMS
Comparison of Control Algorithms for a MEMS

... increase, we can expect increases in the diversity of users and in the number of patients with more challenging optics (i.e. post-surgery, dry eyes, etc.). Improvements in system performance and robustness can significantly increase the clinical and scientific throughput (better quality images from ...
Skin Perfusion Photography
Skin Perfusion Photography

... skin perfusion maps and can be integrated with these methods with, e.g., a high-speed camera or projector. Skin perfusion analysis via LSCI was suggested by Briers [4]. A similar system was suggested later [29]. Analysis of wound healing in pigs was performed recently [30]. Commercial devices [24] e ...
Microscopy Tutorial - Fred Hutch Shared Resources
Microscopy Tutorial - Fred Hutch Shared Resources

... series of sinusoidal ripples. Similarly, a beam of light passing through a small pinhole is not projected as a discrete point, but rather as a larger disc surrounded by concentric rings of decreasing intensity (Airy pattern). These rings are generated by interference of the different diffracted rays ...
Optical Detection of Early Damage in Retinal Ganglion Cells in a
Optical Detection of Early Damage in Retinal Ganglion Cells in a

... ultrastructural properties of tissue and thus has been applied to detect various diseases associated with ultrastructural alterations in their early stages. This study aims to test whether ELBS can detect early damage in retinal ganglion cells (RGCs). METHODS. We used a mouse model of partial optic ...
SPDM: light microscopy with single
SPDM: light microscopy with single

... means that most of the molecules within this nanostructure have an axial distance from each other not exceeding 30 nm [11]. Consequently, the smallest resolvable axial distance (or z-resolution) would also be around 30 nm; if a significant number of molecules would have a larger z-distance from each ...
Wave Optics Theory and 3-D Deconvolution for the Light Field
Wave Optics Theory and 3-D Deconvolution for the Light Field

... sub-pixel movements. During each acquisition, high frequency information from the scene is aliased and recorded as low frequency image features in a way that is uniquely determined by the camera position. If the camera’s trajectory can be accurately estimated, then the different, aliased copies of t ...
Computational photography with plenoptic camera and light field
Computational photography with plenoptic camera and light field

... we have witnessed great technological progress in optics and recording mediums, with digital sensors replacing photographic films in most instances. The latest revolution is computational photography, which seeks to make image reconstruction computation an integral part of the image formation proces ...
Dynamic lighting system for specular surface inspection
Dynamic lighting system for specular surface inspection

... inspection of highly reflective surfaces industrial parts. The basic principle and the application have been exposed. The revealing of aspect defects is effective. This system provides very contrasted images where defect segmentation is elementary. The real time inspection is then possible. A method ...
Wave optics theory and 3-D deconvolution
Wave optics theory and 3-D deconvolution

... optical signal below the Shannon-Nyquist limit, causing high frequency features to be aliased in the light field. In conventional imaging, such aliasing is undesirable because it irreversibly corrupts the recorded image. However, in light field imaging, aliasing is actually beneficial. In particula ...
DENTAL RADIOGRAPHIC TECHNIQUES FOR THE HORSE
DENTAL RADIOGRAPHIC TECHNIQUES FOR THE HORSE

... The mouth is opened fully with a speculum or bite block. The plate/sensor is placed on the side of the horse’s head, with the center of the plate over the rostral end of the facial crest. The clinical crowns of the mandibular cheek teeth are imaged by directing the central beam 10-30 degrees to the ...
Syllabus
Syllabus

... student in all academic work. The guiding principle of academic integrity is that a student’s submitted work must be the student’s own.” Unless otherwise noted by the instructor, work for all assignments in this course must be conducted independently by each student. CO-AUTHORED WORK OF ANY KIND IS ...
View
View

... • Production of a magnified image of the lamp filament that is focused at the level of the aperture plane of the condensor. • Opening or closing this diaphragm controls the angle of the light cone that reaches the specimen, which is determining the image resolution along with the numerical aperture ...


... PMMA and the photoresist have only negligible effects on the imaging quality. Although the illumination is normal to the plane of the object, the subwavelength object scatters the incident radiation in different directions. Two types of polarization for the scattered waves in reference to the plane ...
Three-dimensional imaging of a phase object from a single sample... Chien-Chun Chen, Huaidong Jiang, Lu Rong,
Three-dimensional imaging of a phase object from a single sample... Chien-Chun Chen, Huaidong Jiang, Lu Rong,

... was first measured and then directly phased to obtain an image. The well-known phase problem was solved by oversampling the diffraction intensity2,3 in combination of iterative algorithms.4–7 Using synchrotron radiation, high harmonic generation, soft x-ray laser sources, and free electron lasers, c ...
Fast-Fourier-domain delay line for in vivo optical coherence
Fast-Fourier-domain delay line for in vivo optical coherence

... Optical coherence tomography 共OCT兲 is an imaging modality capable of resolving micrometer-scale features within living biological tissue.1–5 One of the current technological challenges in OCT is to achieve real-time imaging rates with a minimal sacrifice in signal-to-noise ratio 共SNR兲, resolution, a ...
Technology for a better society
Technology for a better society

... the specimen structure. Since the CTF depends very sensitively on defocus, and in general shows an oscillatory behavior as a function of k, the contribution of the different scattered beams to the amplitude modulation varies. However, for particular underfocus settings the instrument approaches a pe ...
rtf
rtf

... Imaging Theory In an electron microscope the specimen scatters the electron wave and the lenses produce the image. The two are very different processes and have to be dealt with quite separately. The reason why imaging is such an important area is that images can lie. As human beings we are conditio ...
``Interaction-free`` imaging - Vienna Center for Quantum Science and
``Interaction-free`` imaging - Vienna Center for Quantum Science and

... of 90%! because both beams undergo the same spatial inversion at their respective mirrors. However, as for system 2, it is not possible to image exactly at the waist. The Michelson systems were investigated chiefly because of the perceived advantages of their relative ease of alignment. However, reg ...
IOSR Journal of Applied Physics (IOSR-JAP)
IOSR Journal of Applied Physics (IOSR-JAP)

... emitted by object and its surrounding. Due to its reliance on variation of self-emitted radiation of scene contents rather than reflected radiation. It is superior to conventional cameras in many respects. It can work at any level of visibility, i.e. operate both in day and night conditions and abso ...
Get PDF - OSA Publishing
Get PDF - OSA Publishing

... modelling of low NA imaging systems is beyond the scope of this article. This article is concerned with rigorous vectorial imaging theory. Vectorial theory for calculating fields in the focal region is now well established [3, 4, 1]. Approximate imaging models have been proposed for restricted scatt ...
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Preclinical imaging

Preclinical imaging is the visualization of living animals for research purposes, such as drug development. Imaging modalities have long been crucial to the researcher in observing changes, either at the organ, tissue, cell, or molecular level, in animals responding to physiological or environmental changes. Imaging modalities that are non-invasive and in vivo have become especially important to study animal models longitudinally. Broadly speaking, these imaging systems can be categorized into primarily morphological/anatomical and primarily molecular imaging techniques. Techniques such as high-frequency micro-ultrasound, magnetic resonance imaging (MRI) and computed tomography (CT) are usually used for anatomical imaging, while optical imaging (fluorescence and bioluminescence), positron emission tomography (PET), and single photon emission computed tomography (SPECT) are usually used for molecular visualizations.These days, many manufacturers provide multi-modal systems combining the advantages of anatomical modalities such as CT and MR with the functional imaging of PET and SPECT. As in the clinical market, common combinations are SPECT/CT, PET/CT and PET/MR.
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