Acetate kinase from CIostridiurn acetobutylicurn : a highly specific
... loaded onto a C 10/20 column containing 8 ml Procion Blue HE3B (Pharmacia) equilibrated with imidazole buffer. The column was washed with 40 ml of the same buffer, and the enzyme was eluted by an ATP gradient (0-1 mM ATP in imidazole buffer; 80 ml) at a maximal flow rate of 10 ml h-'. The fractions ...
... loaded onto a C 10/20 column containing 8 ml Procion Blue HE3B (Pharmacia) equilibrated with imidazole buffer. The column was washed with 40 ml of the same buffer, and the enzyme was eluted by an ATP gradient (0-1 mM ATP in imidazole buffer; 80 ml) at a maximal flow rate of 10 ml h-'. The fractions ...
novel nucleotide carrier proteins of Protochlamydia
... Substrate affinities and mode of transport of PamNTT2, PamNTT3 and PamNTT5 To interpret the function of a transport protein within the physiological context of a cell, it is important to know its apparent substrate affinities. Therefore, we analysed the change of nucleotide transport velocities of P ...
... Substrate affinities and mode of transport of PamNTT2, PamNTT3 and PamNTT5 To interpret the function of a transport protein within the physiological context of a cell, it is important to know its apparent substrate affinities. Therefore, we analysed the change of nucleotide transport velocities of P ...
Predicting the Secondary Structure of Globular Proteins Using
... for which no homologous structures are available. The known structures implicitly contain information about the biophysical properties of amino acids and their interactions. This approach is not meant to be an alternative to other methods that have been developed t,o study protein folding that take ...
... for which no homologous structures are available. The known structures implicitly contain information about the biophysical properties of amino acids and their interactions. This approach is not meant to be an alternative to other methods that have been developed t,o study protein folding that take ...
Posttranslational Protein Modiications in Plant
... Individual proteins often carry multiple PTMs, which pose specific experimental and bioinformatics challenges, as illustrated nicely in a community-wide study (Chalkley et al., 2014). Since many PTMs are reversible, specific residues can also alternate between PTMs (e.g. dependent on cellular conditio ...
... Individual proteins often carry multiple PTMs, which pose specific experimental and bioinformatics challenges, as illustrated nicely in a community-wide study (Chalkley et al., 2014). Since many PTMs are reversible, specific residues can also alternate between PTMs (e.g. dependent on cellular conditio ...
Posttranslational Protein Modiications in Plant
... Individual proteins often carry multiple PTMs, which pose specific experimental and bioinformatics challenges, as illustrated nicely in a community-wide study (Chalkley et al., 2014). Since many PTMs are reversible, specific residues can also alternate between PTMs (e.g. dependent on cellular conditio ...
... Individual proteins often carry multiple PTMs, which pose specific experimental and bioinformatics challenges, as illustrated nicely in a community-wide study (Chalkley et al., 2014). Since many PTMs are reversible, specific residues can also alternate between PTMs (e.g. dependent on cellular conditio ...
Molecular Weight and the Metal Amino Acid Chelate
... “chelate” suppliers stating that their products are formed from metal salts and hydrolyzed protein or amino acids from hydrolyzed protein. The ability to interchange words like hydrolyzed protein or amino acids from hydrolyzed protein has lead to a wide disparity as to what those suppliers call “met ...
... “chelate” suppliers stating that their products are formed from metal salts and hydrolyzed protein or amino acids from hydrolyzed protein. The ability to interchange words like hydrolyzed protein or amino acids from hydrolyzed protein has lead to a wide disparity as to what those suppliers call “met ...
Gene encoding the group B streptococcal protein R4, its
... classification of Rib as a pepsin resistant protein at pH4, it was important to first study the activity of pepsin in relation to pepsin sensitivity testing; second, and more importantly, to determine the gene sequence encoding the R4 protein; and third to determine if the gene sequence could be det ...
... classification of Rib as a pepsin resistant protein at pH4, it was important to first study the activity of pepsin in relation to pepsin sensitivity testing; second, and more importantly, to determine the gene sequence encoding the R4 protein; and third to determine if the gene sequence could be det ...
Full-Text PDF
... increase of the material–liquid ratio showed no significant differences in the hydroxyl scavenging rate. However, when the material–liquid ratio exceeded 1:10, the DPPH radical scavenging rate of hydrolysate decreased sharply. Moreover, peptide yield remained stable with increasing degrees of hydrol ...
... increase of the material–liquid ratio showed no significant differences in the hydroxyl scavenging rate. However, when the material–liquid ratio exceeded 1:10, the DPPH radical scavenging rate of hydrolysate decreased sharply. Moreover, peptide yield remained stable with increasing degrees of hydrol ...
proposal-aug25
... short conserved segments that fall within disordered regions, but do not match any known protein domains. To estimate the expected number of false positives identified in the cluster analysis we will repeat the clustering on the motifs identified in simulated disordered proteins. These computational ...
... short conserved segments that fall within disordered regions, but do not match any known protein domains. To estimate the expected number of false positives identified in the cluster analysis we will repeat the clustering on the motifs identified in simulated disordered proteins. These computational ...
Channel Protein From Rat Heart Using Subtype
... was washed extensively with buffer B and eluted with buffer containing 400 mmol/L KCI, 20 mmol/L potassium phosphate (pH 6.5), 0.5 mmol/L MgC12, 1 mmol/L EGTA, 0.1 mmol/L PMSF, 0.1 ,ug/mL pepstatin A, and 0.1% NP-40 (buffer C). The eluate from the DEAE Sephadex was applied to a column of wheat germ ...
... was washed extensively with buffer B and eluted with buffer containing 400 mmol/L KCI, 20 mmol/L potassium phosphate (pH 6.5), 0.5 mmol/L MgC12, 1 mmol/L EGTA, 0.1 mmol/L PMSF, 0.1 ,ug/mL pepstatin A, and 0.1% NP-40 (buffer C). The eluate from the DEAE Sephadex was applied to a column of wheat germ ...
Types and effects of protein variations. Vihinen
... interactions and other characteristics in several ways. List of the effects would be really long and include e.g. interactions, stability, electrostatic effects, protein packing, local and global structural changes and so on. Many deleterious variants are straightforward to explain, such as large de ...
... interactions and other characteristics in several ways. List of the effects would be really long and include e.g. interactions, stability, electrostatic effects, protein packing, local and global structural changes and so on. Many deleterious variants are straightforward to explain, such as large de ...
Introduction to Qualitative Analysis
... compound could be used to reveal that iron, potassium and oxalate ions are present. It could also be used to determine that the iron is in its +3 oxidation state and that the substance is a hydrate. On the other hand, quantitative analysis of the green crystals would reveal that it contains 11.4% Fe ...
... compound could be used to reveal that iron, potassium and oxalate ions are present. It could also be used to determine that the iron is in its +3 oxidation state and that the substance is a hydrate. On the other hand, quantitative analysis of the green crystals would reveal that it contains 11.4% Fe ...
Anchoring of Surface Proteins to the Cell Wall of Staphylococcus
... mutant derivatives) were transformed into E. coli XL-1 Blue, and transformants selected on LB agar containing ampicillin. Mutant plasmids were purified, and the presence or absence of nucleotide changes was determined by DNA sequencing (Table I). Purification of Recombinant Sortase: E. coli—XL-1 Blu ...
... mutant derivatives) were transformed into E. coli XL-1 Blue, and transformants selected on LB agar containing ampicillin. Mutant plasmids were purified, and the presence or absence of nucleotide changes was determined by DNA sequencing (Table I). Purification of Recombinant Sortase: E. coli—XL-1 Blu ...
COMPUTATIONAL PERSPECTIVE IN THE STRUCTURAL STABILITY OF ‘ALLALPHA’ PROTEINS: THE NH...Π INTERACTIONS
... The values of S obtained for all the amino acids were plotted in Fig. 3. The percentage ratio calculated shows that Arg make the maximum contribution to this N‐H...π interaction (52 interactions in a total of 160 interactions). It might be due to the fact that the side chain of arginin ...
... The values of S obtained for all the amino acids were plotted in Fig. 3. The percentage ratio calculated shows that Arg make the maximum contribution to this N‐H...π interaction (52 interactions in a total of 160 interactions). It might be due to the fact that the side chain of arginin ...
Characterisation of Milk Proteins in Confectionery Products
... to identify and compare the form and location of milk proteins in three different confectionery products. Secondly, to investigate how proteins from different milk sources and various proces ses have performed in the products. Following initial light microscopy studies, electron microscopy has been ...
... to identify and compare the form and location of milk proteins in three different confectionery products. Secondly, to investigate how proteins from different milk sources and various proces ses have performed in the products. Following initial light microscopy studies, electron microscopy has been ...
Adv. Protein Chem. Struct. Biol.
... times for each spatial rotation of the crystal and many times for the same time delay. The applicability of the method depends on whether the molecule retains its biological activity in the crystalline state, whether the molecule is inherently photosensitive or if it could be engineered as so, wheth ...
... times for each spatial rotation of the crystal and many times for the same time delay. The applicability of the method depends on whether the molecule retains its biological activity in the crystalline state, whether the molecule is inherently photosensitive or if it could be engineered as so, wheth ...
The role of IRES trans-acting factors in regulating translation initiation
... next [31]. However, a few interactions between cellular IRESs and their cognate ITAFs have been defined. In particular, the Apaf-1 (apoptotic peptidase-activating factor 1) and Bag-1 (Bcl-2-associated athanogene-1) IRESs have been extensively studied in this regard, and the data suggest that ITAFs r ...
... next [31]. However, a few interactions between cellular IRESs and their cognate ITAFs have been defined. In particular, the Apaf-1 (apoptotic peptidase-activating factor 1) and Bag-1 (Bcl-2-associated athanogene-1) IRESs have been extensively studied in this regard, and the data suggest that ITAFs r ...
and gender-specific hallmarks
... coverage and results in a poor representation of low-abundant or very hydrophobic proteins as well as those with extreme pI and molecular weight13,14. On the other hand, current high-throughput proteomic techniques enable reliable detection and quantitation of thousands of proteins in complex proteo ...
... coverage and results in a poor representation of low-abundant or very hydrophobic proteins as well as those with extreme pI and molecular weight13,14. On the other hand, current high-throughput proteomic techniques enable reliable detection and quantitation of thousands of proteins in complex proteo ...
Intrinsically Disordered Protein - Center for Data Analytics and
... extraordinary diverse and specific interactions with one another and with other substances, can be explained on the basis of a few relatively rigid general patterns, simply by varying the nature and sequence of the side chains attached to the fundamental repeating pattern?”.8 This idea took firm roo ...
... extraordinary diverse and specific interactions with one another and with other substances, can be explained on the basis of a few relatively rigid general patterns, simply by varying the nature and sequence of the side chains attached to the fundamental repeating pattern?”.8 This idea took firm roo ...
Identification of fungal oxaloacetate hydrolyase within the
... ability of fungi to produce oxalate with the presence of an oah gene in their genome. This task was made difficult by the fact that fungal genomes encode several OAH homologs having an unusually high level of shared sequence identity. Thus, identification of OAH activity in the gene product would re ...
... ability of fungi to produce oxalate with the presence of an oah gene in their genome. This task was made difficult by the fact that fungal genomes encode several OAH homologs having an unusually high level of shared sequence identity. Thus, identification of OAH activity in the gene product would re ...
Ligand Conformation Enforces Trigonal
... dinuclear cations [Cu(Npy2pz)]22+, both located on an inversion center. Figure 2 shows an ORTEP view of one of the independent molecules. The main angles and bond distances are presented in Table 2. The coordination geometry around the copper(II) atoms is trigonal bipyramidal, with the copper ions b ...
... dinuclear cations [Cu(Npy2pz)]22+, both located on an inversion center. Figure 2 shows an ORTEP view of one of the independent molecules. The main angles and bond distances are presented in Table 2. The coordination geometry around the copper(II) atoms is trigonal bipyramidal, with the copper ions b ...
characterisation of novel proteins
... gram positive sporulating soil bacteria. Few Streptomyces have been isolated from animal or human sources and pathogenicity is not a typical property of these organisms. S. viridochromogenes is itself not known to be a human pathogen and nor has it been associated with other properties (e.g. product ...
... gram positive sporulating soil bacteria. Few Streptomyces have been isolated from animal or human sources and pathogenicity is not a typical property of these organisms. S. viridochromogenes is itself not known to be a human pathogen and nor has it been associated with other properties (e.g. product ...